LOCUS       sequence-17078-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Bastians H, Topper LM, Gorbsky GL, Ruderman JV
  TITLE     Cell cycle-regulated proteolysis of mitotic target proteins.
  JOURNAL   Mol Biol Cell 1999 Nov;10(11):3927-41.
  PUBMED    10564281
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell"; date: "1999-11"; volume: "10(11)"; pages: "3927-41"
COMMENT     Sequence Label: pET-UbcH10-DN
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             589..606
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(659..677)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      673..720
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aattccctct gaatattttg tttactttaa gaaggagata tacatatggc ttcccaaaac
       61 cgcgacccag ccgccactag cgtcgccgcc gcccgtaaag gagctgagcc gagcgggggc
      121 gccgcccggg gtccggtggg caaaaggcta cagcaggagc tgatgaccct catgatgtct
      181 ggcgataaag ggatttctgc cttccctgaa tcagacaacc ttttcaaatg ggtagggacc
      241 atccatggag cagctggaac agtatatgaa gacctgaggt ataagctctc gctagagttc
      301 cccagtggct acccttacaa tgcgcccaca gtgaagttcc tcacgccctg ctatcacccc
      361 aacgtggaca cccagggtaa cataagcctg gacatcctga aggaaaagtg gtctgccctg
      421 tatgatgtca ggaccattct gctctccatc cagagccttc taggagaacc caacattgat
      481 agtcccttga acacacatgc tgccgagctc tggaaaaacc ccacagcttt taagaagtac
      541 ctgcaagaaa cctactcaaa gcaggtcacc agccaggagc ccctcgagca ccaccaccac
      601 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      661 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
      721 ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa
      781 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      841 ccgctccttt cgcttttctt cccttccttt ctcgccacgt tcgccggctt tccccgtca
//
