LOCUS       sequence-170784-        2802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Halo-His6 tagged Nrf2 for E.coli expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Parvez S, Fu Y, Li J, Long MJ, Lin H, Lee DK, Hu GS, Aye Y
  TITLE     Substoichiometric Hydroxynonenylation of a Single Protein 
            Recapitulates Whole-Cell-Stimulated Antioxidant Response.
  JOURNAL   J Am Chem Soc. 2014 Dec 31.
  PUBMED    25544059
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2014 Dec 31."
COMMENT     Sequence Label: pet28a-His6-Halo-TEV-Nrf2
FEATURES             Location/Qualifiers
     source          1..2802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             61..951
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             958..978
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgcctcg tggtagccat
       61 atggcagaaa tcggtactgg ctttccattc gacccccatt atgtggaagt cctgggcgag
      121 cgcatgcact acgtcgatgt tggtccgcgc gatggcaccc ctgtgctgtt cctgcacggt
      181 aacccgacct cctcctacgt gtggcgcaac atcatcccgc atgttgcacc gacccatcgc
      241 tgcattgctc cagacctgat cggtatgggc aaatccgaca aaccagacct gggttatttc
      301 ttcgacgacc acgtccgctt catggatgcc ttcatcgaag ccctgggtct ggaagaggtc
      361 gtcctggtca ttcacgactg gggctccgct ctgggtttcc actgggccaa gcgcaatcca
      421 gagcgcgtca aaggtattgc atttatggag ttcatccgcc ctatcccgac ctgggacgaa
      481 tggccagaat ttgcccgcga gaccttccag gccttccgca ccaccgacgt cggccgcaag
      541 ctgatcatcg atcagaacgt ttttatcgag ggtacgctgc cgatgggtgt cgtccgcccg
      601 ctgactgaag tcgagatgga ccattaccgc gagccgttcc tgaaccctgt tgaccgcgag
      661 ccactgtggc gcttcccaaa cgagctgcca atcgccggtg agccagcgaa catcgtcgcg
      721 ctggtcgaag aatacatgga ctggctgcac cagtcccctg tcccgaagct gctgttctgg
      781 ggcaccccag gcgttctgat cccaccggcc gaagccgctc gcctggccaa aagcctgcct
      841 aactgcaagg ctgtggacat cggcccgggt ctgaatctgc tgcaagaaga caacccggac
      901 ctgatcggca gcgagatcgc gcgctggctg tcgacgctcg agatttccgg ctccggagaa
      961 aacttgtatt tccagggctc agggatgatg gacttggagc tgccgccgcc gggactcccg
     1021 tcccagcagg acatggattt gattgacata ctttggaggc aagatataga tcttggagta
     1081 agtcgagaag tatttgactt cagtcagcga cggaaagagt atgagctgga aaaacagaaa
     1141 aaacttgaaa aggaaagaca agaacaactc caaaaggagc aagagaaagc ctttttcgct
     1201 cagttacaac tagatgaaga gacaggtgaa tttctcccaa ttcagccagc ccagcacatc
     1261 cagtcagaaa ccagtggatc tgccaactac tcccaggttg cccacattcc caaatcagat
     1321 gctttgtact ttgatgactg catgcagctt ttggcgcaga cattcccgtt tgtagatgac
     1381 aatgaggttt cttcggctac gtttcagtca cttgttcctg atattcccgg tcacatcgag
     1441 agcccagtct tcattgctac taatcaggct cagtcacctg aaacttctgt tgctcaggta
     1501 gcccctgttg atttagacgg tatgcaacag gacattgagc aagtttggga ggagctatta
     1561 tccattcctg agttacagtg tcttaatatt gaaaatgaca agctggttga gactaccatg
     1621 gttccaagtc cagaagccaa actgacagaa gttgacaatt atcattttta ctcatctata
     1681 ccctcaatgg aaaaagaagt aggtaactgt agtccacatt ttcttaatgc ttttgaggat
     1741 tccttcagca gcatcctctc cacagaagac cccaaccagt tgacagtgaa ctcattaaat
     1801 tcagatgcca cagtcaacac agattttggt gatgaatttt attctgcttt catagctgag
     1861 cccagtatca gcaacagcat gccctcacct gctactttaa gccattcact ctctgaactt
     1921 ctaaatgggc ccattgatgt ttctgatcta tcactttgca aagctttcaa ccaaaaccac
     1981 cctgaaagca cagcagaatt caatgattct gactccggca tttcactaaa cacaagtccc
     2041 agtgtggcat caccagaaca ctcagtggaa tcttccagct atggagacac actacttggc
     2101 ctcagtgatt ctgaagtgga agagctagat agtgcccctg gaagtgtcaa acagaatggt
     2161 cctaaaacac cagtacattc ttctggggat atggtacaac ccttgtcacc atctcagggg
     2221 cagagcactc acgtgcatga tgcccaatgt gagaacacac cagagaaaga attgcctgta
     2281 agtcctggtc atcggaaaac cccattcaca aaagacaaac attcaagccg cttggaggct
     2341 catctcacaa gagatgaact tagggcaaaa gctctccata tcccattccc tgtagaaaaa
     2401 atcattaacc tccctgttgt tgacttcaac gaaatgatgt ccaaagagca gttcaatgaa
     2461 gctcaacttg cattaattcg ggatatacgt aggaggggta agaataaagt ggctgctcag
     2521 aattgcagaa aaagaaaact ggaaaatata gtagaactag agcaagattt agatcatttg
     2581 aaagatgaaa aagaaaaatt gctcaaagaa aaaggagaaa atgacaaaag ccttcaccta
     2641 ctgaaaaaac aactcagcac cttatatctc gaagttttca gcatgctacg tgatgaagat
     2701 ggaaaacctt attctcctag tgaatactcc ctgcagcaaa caagagatgg caatgttttc
     2761 cttgttccca aaagtaagaa gccagatgtt aagaaaaact ag
//
