LOCUS       sequence-170808-        8478 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes gRNA for 3' target of human HLF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8478)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 8478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: PX458_HLF_1
FEATURES             Location/Qualifiers
     source          1..8478
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..67
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             74..94
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             119..4219
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             119..4219
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4220..4267
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4283..4336
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4337..4339,4340..5050)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4337..4339,4340..5050)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4640..4659)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4987..5008
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5078..5095)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5084..5291
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5300..5440
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5300..5429
                     /label=AAV2 ITR
     rep_origin      5515..5970
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5602..5621)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5812..5833
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5987..6006)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6106..6128
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6166..6184)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6252..6356
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6357..6425,6426..7217)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6575..6594)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7388..7976
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7877..7896
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8038..8286
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8038..8058
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8209..8228
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8307..8382
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggactataag gaccacgacg gagactacaa ggatcatgat attgattaca aagacgatga
       61 cgataagatg gccccaaaga agaagcggaa ggtcggtatc cacggagtcc cagcagccga
      121 caagaagtac agcatcggcc tggacatcgg caccaactct gtgggctggg ccgtgatcac
      181 cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag
      241 catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac
      301 ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct
      361 gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga
      421 agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat
      481 cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact
      541 ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat
      601 caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga
      661 caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa
      721 cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct
      781 ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gaaacctgat
      841 tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc
      901 caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat
      961 cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct
     1021 gagcgacatc ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat
     1081 caagagatac gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca
     1141 gctgcctgag aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta
     1201 cattgacggc ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa
     1261 gatggacggc accgaggaac tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca
     1321 gcggaccttc gacaacggca gcatccccca ccagatccac ctgggagagc tgcacgccat
     1381 tctgcggcgg caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa
     1441 gatcctgacc ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt
     1501 cgcctggatg accagaaaga gcgaggaaac catcaccccc tggaacttcg aggaagtggt
     1561 ggacaagggc gcttccgccc agagcttcat cgagcggatg accaacttcg ataagaacct
     1621 gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtataa
     1681 cgagctgacc aaagtgaaat acgtgaccga gggaatgaga aagcccgcct tcctgagcgg
     1741 cgagcagaaa aaggccatcg tggacctgct gttcaagacc aaccggaaag tgaccgtgaa
     1801 gcagctgaaa gaggactact tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg
     1861 cgtggaagat cggttcaacg cctccctggg cacataccac gatctgctga aaattatcaa
     1921 ggacaaggac ttcctggaca atgaggaaaa cgaggacatt ctggaagata tcgtgctgac
     1981 cctgacactg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct atgcccacct
     2041 gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct ggggcaggct
     2101 gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa tcctggattt
     2161 cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag ctgatccacg acgacagcct
     2221 gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata gcctgcacga
     2281 gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc agacagtgaa
     2341 ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca tcgtgatcga
     2401 aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaatgaa
     2461 gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac accccgtgga
     2521 aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg ggcgggatat
     2581 gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg accatatcgt
     2641 gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca gaagcgacaa
     2701 gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta
     2761 ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca atctgaccaa
     2821 ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga gacagctggt
     2881 ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga tgaacactaa
     2941 gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga agtccaagct
     3001 ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca acaactacca
     3061 ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc
     3121 taagctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat
     3181 cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct acagcaacat
     3241 catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct
     3301 gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg attttgccac
     3361 cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac
     3421 aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc tgatcgccag
     3481 aaagaaggac tgggacccta agaagtacgg cggcttcgac agccccaccg tggcctattc
     3541 tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga
     3601 gctgctgggg atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct
     3661 ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc
     3721 cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccggcg aactgcagaa
     3781 gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta
     3841 tgagaagctg aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca
     3901 caagcactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct
     3961 ggccgacgct aatctggaca aagtgctgtc cgcctacaac aagcaccggg ataagcccat
     4021 cagagagcag gccgagaata tcatccacct gtttaccctg accaatctgg gagcccctgc
     4081 cgccttcaag tactttgaca ccaccatcga ccggaagagg tacaccagca ccaaagaggt
     4141 gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct
     4201 gtctcagctg ggaggcgaca aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa
     4261 gaaaaaggaa ttcggcagtg gagagggcag aggaagtctg ctaacatgcg gtgacgtcga
     4321 ggagaatcct ggcccagtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     4381 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     4441 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     4501 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     4561 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     4621 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     4681 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     4741 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     4801 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     4861 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     4921 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     4981 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     5041 gctgtacaag gaattctaac tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
     5101 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     5161 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     5221 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga gaatagcagg
     5281 catgctgggg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     5341 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     5401 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
     5461 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
     5521 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5581 ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5641 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5701 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     5761 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     5821 tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga
     5881 ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     5941 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     6001 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     6061 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     6121 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     6181 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     6241 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6301 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6361 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6421 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6481 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6541 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6601 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6661 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6721 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6781 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6841 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6901 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6961 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     7021 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt ggaagccgcg
     7081 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     7141 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     7201 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     7261 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7321 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7381 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7441 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7501 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     7561 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7621 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7681 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7741 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7801 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7861 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7921 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7981 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtgag
     8041 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
     8101 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
     8161 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
     8221 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     8281 aacaccgcaa aaatgccatc ctacaggttt tagagctaga aatagcaagt taaaataagg
     8341 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttgt tttagagcta
     8401 gaaatagcaa gttaaaataa ggctagtccg tttttagcgc gtgcgccaat tctgcagaca
     8461 aatggctcta gaggtacc
//
