LOCUS       sequence-170811-        9342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Y2H Gal4 DNA binding domain for Clf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9342)
  AUTHORS   Carrocci TJ, Zoerner DM, Paulson JC, Hoskins AA
  TITLE     SF3b1 mutations associated with myelodysplastic syndromes alter the 
            fidelity of branchsite selection in yeast.
  JOURNAL   Nucleic Acids Res. 2017 Jan 6. pii: gkw1349. doi: 
            10.1093/nar/gkw1349.
  PUBMED    28062854
REFERENCE   2  (bases 1 to 9342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2017 Jan 6. pii: gkw1349. doi: 10.1093/nar/gkw1349."
COMMENT     Sequence Label: pGBKT7-CLF1
FEATURES             Location/Qualifiers
     source          1..9342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..734
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             762..1202
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     1213..1232
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1213..1231
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1251..1280
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      3377..3424
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      3451..3638
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3506..3530)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3662..3678)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3662..3678)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3675..3697)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3686..3702
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3710..3716,3717..3734,3735..3740))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3755..3776
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3893..3910)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4064..4652)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4144..4163)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4981..5028
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5053..5072
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5260..6054)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5260..6054)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5371..5390)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5981..6000
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6055..6159)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6186..7528
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(7149..7196)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     7569..7587
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7625..7647)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7747..7766
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7787..8068
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             8069..8743
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(8846..9301)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8983..9004)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9195..9214
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg
       61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
      121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
      181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
      241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
      301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
      361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat gatggaagac
      421 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
      481 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc
      541 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaaagtt
      601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
      661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
      721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta
      781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga
      841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt
      901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc
      961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac
     1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg
     1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa
     1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat
     1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc
     1261 tgatctcaga ggaggacctg catatggaca ctttagagcc aacagccgtt gatacgcatg
     1321 tttctgcgga gcagattctg cgggatgtgt acaagaaggg gcaaaaagca agaggatcga
     1381 ctaatattga tatattagac ttggaggaac ttcgagagta tcaacgaagg aagagaacag
     1441 aatatgaggg ttatttgaag agaaaccgac tggatatggg acaatggatt cgttatgcgc
     1501 aattcgaaat cgaacaacac gacatgaggc gcgccagatc tatctttgaa agagcattat
     1561 tagtagatag ttcattcatc cccctttgga tacgatacat tgatgctgag ctgaaagtca
     1621 aatgtattaa ccatgctagg aatcttatga acagagctat tagtacgttg cccagagtag
     1681 acaagttatg gtacaagtat ctcattgtcg aagagtcgtt aaacaacgtt gaaattgtac
     1741 gatcactgta caccaaatgg tgctccttgg agccaggggt taatgcatgg aattctttcg
     1801 tggattttga aataagacag aaaaactgga acggtgtaag agagatttac tcaaaatacg
     1861 tcatggctca cccgcaaatg cagacatggc taaagtgggt gaggtttgaa aatagacatg
     1921 gtaatactga atttacgaga agtgtgtatt ctttagcgat agacacagta gctaacctcc
     1981 aaaatttaca gatttggtct gatatggagg ttgcaaaatt agtaaattca tttgctcatt
     2041 gggaagctgc gcagcaagaa tacgaaagat ctagtgctct ttatcaaatt gctatagaaa
     2101 aatggccctc caatcaattg ctcaaggcag gactgttgga ttttgaaaag cagtttggtg
     2161 acataaactc aatagaagaa actattagct ataaaaggaa aatggagtat gaaacaatat
     2221 tgagtaataa cgcctatgat tatgacacct ggtggctgta cttggatctc atttcagaaa
     2281 gtttccccaa acaaataatg caaactttcg agaaagctat tgtggacagc cgaccaaaag
     2341 aactatcaaa aaatgttcag tggaaaaggt atatttatct gtggatgaga tatatatgct
     2401 atgttgagtt agaattggaa aattcgcttt tagaagaaga actttttcag aggttaattg
     2461 acgacatcat ccctcataaa catttcactt tttccaaaat atggctaatg tatgctaaat
     2521 ttctgatacg acatgatgat gtaccgaagg cgagaaaaat tttgggtaaa gcaatcggtt
     2581 tatgtcctaa ggctaaaact ttcaaaggat atatcgagtt ggaagtgaag ttgaaggaat
     2641 ttgatcgtgt gagaaaaata tatgagaaat ttattgaatt tcagccctct gatctacaaa
     2701 tttggtcaca atatggagaa ctagaagaaa atttaggaga ttgggataga gtaagaggta
     2761 tatatacaat tgcactagat gaaaattctg atttcttaac aaaggaggct aaaattgtat
     2821 tacttcaaaa atacataaca tttgagacag aatcacaaga atttgaaaag gcaaggaagc
     2881 tttatagaag atatttggag ctcaaccaat attcccccca aagctggatc gaattcgcca
     2941 tgtatcaaac ttcaaccccg acggagcaac aactattaga tttagcaaaa ctgcaaagcg
     3001 aaaatgtgga tgaagacata gagtttgaaa ttaccgatga gaataaattg gaggcaagaa
     3061 aagtttttga agaggcgata gtttttttca aggagaagga tgataaacaa gggagactgt
     3121 ctattttgga agcgctaaaa gactatgaag aaacttacgg tactgaactc gatcaagaaa
     3181 ccgtcaagaa aagattccct aaagtaatca aaaaagtgag gttacagaac ggtgtggaag
     3241 aagaattcgt ggattacatt tttcctgatg atatcgatga tgacaagcca aaaccatcaa
     3301 agttcttgga actagccaaa aaatggaaac aggaacaggc actttgagga tccgtcgacc
     3361 tgcagcggcc gcataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     3421 tttgcgcgct tgcagccaag ctaattccgg gcgaatttct tatgatttat gatttttatt
     3481 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
     3541 aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
     3601 tagcatgagg tcgctcttat tgaccacacc tctaccggca tgcaagcttg gcgtaatcat
     3661 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     3721 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     3781 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     3841 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     3901 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3961 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4021 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4081 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4141 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4201 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4261 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4321 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4381 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4441 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4501 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4561 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4621 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     4681 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4741 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4801 atgagtaacc tgaggctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc
     4861 cttcacccga acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc
     4921 aatggggtct cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat
     4981 gaacaaacga cccaacaccg tgcgttttat tctgtctttt tattgccgtc atagcgcggg
     5041 ttccttccgg tattgtctcc ttccgtgttt cagttagcct ccccctaggg tgggcgaaga
     5101 actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc
     5161 cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg
     5221 gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag
     5281 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     5341 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     5401 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     5461 caccatgata ttcggcaagc aggcatcgcc atgagtcacg acgagatcct cgccgtcggg
     5521 catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     5581 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     5641 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     5701 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     5761 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     5821 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc
     5881 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     5941 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     6001 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatactctt
     6061 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6121 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6181 acctgaacga agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa
     6241 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc
     6301 tattttacca acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc
     6361 gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag
     6421 agcgctattt taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg
     6481 agagcgctat ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta
     6541 taatgcagtc tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac
     6601 tttggtgtct attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat
     6661 tactagcgaa gctgcgggtg cattttttca agataaaggc atccccgatt atattctata
     6721 ccgatgtgga ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg
     6781 gtcagaaaat tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt
     6841 ttacattttc gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt
     6901 ctaaagagta atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt
     6961 caaggagcga aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa
     7021 agagatactt ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta
     7081 cagtccggtg cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa
     7141 agcgctctga agttcctata ctttctagag aataggaact tcggaatagg aacttcaaag
     7201 cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg
     7261 ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca
     7321 tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt
     7381 agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag
     7441 cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca
     7501 atgctatcat ttcctttgat attggatcat actaagaaac cattattatc atgacattaa
     7561 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     7621 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     7681 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     7741 actatgcggc atcagagcag attgtactga gagtgcacca tagatcaacg acattactat
     7801 atatataata taggaagcat ttaatagaac agcatcgtaa tatatgtgta ctttgcagtt
     7861 atgacgccag atggcagtag tggaagatat tctttattga aaaatagctt gtcaccttac
     7921 gtacaatctt gatccggagc ttttcttttt ttgccgatta agaattaatt cggtcgaaaa
     7981 aagaaaagga gagggccaag agggagggca ttggtgacta ttgagcacgt gagtatacgt
     8041 gattaagcac acaaaggcag cttggagtat gtctgttatt aatttcacag gtagttctgg
     8101 tccattggtg aaagtttgcg gcttgcagag cacagaggcc gcagaatgtg ctctagattc
     8161 cgatgctgac ttgctgggta ttatatgtgt gcccaataga aagagaacaa ttgacccggt
     8221 tattgcaagg aaaatttcaa gtcttgtaaa agcatataaa aatagttcag gcactccgaa
     8281 atacttggtt ggcgtgtttc gtaatcaacc taaggaggat gttttggctc tggtcaatga
     8341 ttacggcatt gatatcgtcc aactgcatgg agatgagtcg tggcaagaat accaagagtt
     8401 cctcggtttg ccagttatta aaagactcgt atttccaaaa gactgcaaca tactactcag
     8461 tgcagcttca cagaaacctc attcgtttat tcccttgttt gattcagaag caggtgggac
     8521 aggtgaactt ttggattgga actcgatttc tgactgggtt ggaaggcaag agagccccga
     8581 aagcttacat tttatgttag ctggtggact gacgccagaa aatgttggtg atgcgcttag
     8641 attaaatggc gttattggtg ttgatgtaag cggaggtgtg gagacaaatg gtgtaaaaga
     8701 ctctaacaaa atagcaaatt tcgtcaaaaa tgctaagaaa taggttatta ctgagtagta
     8761 tttatttaag tattgtttgt gcacttgccg atctatgcgg tgtgaaatac cgcacagatg
     8821 cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg
     8881 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     8941 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     9001 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     9061 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     9121 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     9181 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     9241 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     9301 tccattcgcc attcaggctg cgcaactgtt gggaagggcg at
//
