LOCUS       sequence-170828-        9909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Y2H Gal4 DNA binding domain for Prp2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9909)
  AUTHORS   Carrocci TJ, Zoerner DM, Paulson JC, Hoskins AA
  TITLE     SF3b1 mutations associated with myelodysplastic syndromes alter the 
            fidelity of branchsite selection in yeast.
  JOURNAL   Nucleic Acids Res. 2017 Jan 6. pii: gkw1349. doi: 
            10.1093/nar/gkw1349.
  PUBMED    28062854
REFERENCE   2  (bases 1 to 9909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2017 Jan 6. pii: gkw1349. doi: 10.1093/nar/gkw1349."
COMMENT     Sequence Label: pGBKT7-PRP2
FEATURES             Location/Qualifiers
     source          1..9909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..734
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             762..1202
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     1213..1232
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1213..1231
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1251..1280
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      3944..3991
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4018..4205
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4073..4097)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(4229..4245)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4229..4245)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4242..4264)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4253..4269
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4277..4283,4284..4301,4302..4307))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4322..4343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4460..4477)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4631..5219)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4711..4730)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5548..5595
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5620..5639
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5827..6621)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5827..6621)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5938..5957)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6548..6567
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6622..6726)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6753..8095
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(7716..7763)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     8136..8154
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8192..8214)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8314..8333
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8354..8635
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             8636..9310
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(9413..9868)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9550..9571)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9762..9781
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg
       61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
      121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
      181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
      241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
      301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
      361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat gatggaagac
      421 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
      481 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc
      541 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaaagtt
      601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
      661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
      721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta
      781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga
      841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt
      901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc
      961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac
     1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg
     1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa
     1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat
     1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc
     1261 tgatctcaga ggaggacctg catatgtcaa gtattacatc tgaaaccggt aagcgaagag
     1321 tgaaacgaac gtatgaagtt acaagacaga atgacaatgc agtaagaata gagccttctt
     1381 ccttgggtga ggaagaagac aaagaggcca aggataagaa ctctgccttg cagctaaaac
     1441 gatcacgcta tgaccccaat aaagtttttt ctaatacgaa ccaaggtcca gaaaagaaca
     1501 atttaaaagg agaacaactt ggatcacaaa aaaagtcatc aaagtatgat gaaaaaataa
     1561 cgtcaaataa tgaacttact acaaagaagg gtctactagg tgactccgag aatgaaacta
     1621 agtacgcctc ttctaattct aaatttaacg tagaagttac tcataaaata aagaatgcaa
     1681 aagaaattga caaaataaac agacaacgca tgtgggaaga acaacaattg cgaaatgcta
     1741 tggcggggca aagcgatcac cctgatgata taacattgga aggctctgac aagtacgatt
     1801 atgtgttcga cacagatgca atgattgatt atactaatga ggaggacgat ttactacctg
     1861 aagagaaact tcaatatgag gcacgtctag cacaagcttt agaaactgag gaaaagcgta
     1921 tactgactat acaagaggca agaaaacttt tgcctgtaca tcaatacaag gatgagctac
     1981 tacaagagat caaaaaaaat caagttttga ttataatggg tgaaaccggt tctggtaaaa
     2041 ccacacagtt accccaatat ttggtagaag atggattcac tgatcaggga aagttacaga
     2101 ttgccataac acaaccacgt cgtgtggcgg ccacttctgt tgctgcaaga gtagcagatg
     2161 aaatgaatgt tgtgcttggg aaggaagtgg gttatcagat ccgttttgaa gacaaaacaa
     2221 ccccaaataa aacggttcta aagtatatga cggatggtat gttactaaga gaatttctta
     2281 cagattccaa attaagcaaa tattcttgta ttatgattga tgaagctcat gaacgtactc
     2341 tagccacaga tatcctgata ggtttgctga aagatatttt gccgcagagg cccactttaa
     2401 aattgcttat atcatcagca acaatgaacg caaaaaaatt ttcagagttt tttgataatt
     2461 gtcccatttt caacgttcct gggagaagat atcctgtaga tatccactac acattacagc
     2521 cggaggcaaa ctatatacat gccgctataa caactatttt tcaaattcat actacgcaaa
     2581 gtttaccggg ggatatattg gtgtttttaa cggggcaaga agaaattgaa agaacaaaaa
     2641 ccaaactgga ggagatcatg tctaaattag gttctagaac aaaacaaatg attataactc
     2701 ctatatacgc aaatttaccc caagagcagc aattgaaaat tttccagccg actccggaaa
     2761 attgtaggaa agttgttttg gcaactaata ttgcggaaac ttcattgacc attgatggta
     2821 ttagatatgt cattgatcct ggatttgtta aggaaaattc gtacgtaccc tccactggaa
     2881 tgacccaact tttaacagtt ccatgctcaa gagcttcagt tgatcagcgt gccggtaggg
     2941 ctggtcgtgt tgggccggga aaatgtttta gaatttttac caaatggtca tatttgcacg
     3001 agttggaact tatgcctaag cctgagatca caagaacaaa tctttctaat acggtgttat
     3061 tattattatc gcttggcgtt actgacctaa taaagtttcc cctgatggat aagcccagta
     3121 tccccacatt acggaagtct ttggaaaatt tatacatttt aggtgcctta aatagcaaag
     3181 ggacaatcac tcggttaggt aaaatgatgt gcgagtttcc ttgtgaacct gaatttgcaa
     3241 aagtgcttta tacagctgct acgcacgaac aatgtcaagg tgtactagaa gagtgtttga
     3301 ccatagtttc gatgctacat gaaacacctt cattatttat tggtcaaaaa agagatgctg
     3361 cggcaagtgt gttaagcgaa gtggaaagtg atcacatatt gtatttggaa atcttcaacc
     3421 aatggagaaa ctctaaattt tccagaagtt ggtgtcaaga ccacaaaatt caattcaaaa
     3481 cgatgttgag ggtaagaaat attagaaacc aattattcag gtgcagtgaa aaggttggat
     3541 tagttgaaaa gaacgaccaa gccaggatga agataggcaa tatagcgggc tacataaatg
     3601 ctagaataac tcgatgcttc atcagcgggt ttccgatgaa tattgtacaa ttaggcccca
     3661 ccggctatca aaccatgggt aggtcatcag gtgggctgaa cgttagtgtt catcctacca
     3721 gtatactttt cgttaatcat aaggaaaagg cgcagagacc aagcaaatat gtgctttatc
     3781 aacaattgat gctaacgtcg aaggaattta tcagggactg tctagttatc cctaaggaag
     3841 aatggttgat agacatggtt ccacaaatct ttaaagattt aattgacgat aaaacaaata
     3901 gggggaggcg gtgaggatcc gtcgacctgc agcggccgca taactagcat aaccccttgg
     3961 ggcctctaaa cgggtcttga ggggtttttt gcgcgcttgc agccaagcta attccgggcg
     4021 aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac
     4081 aaattttaaa gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc
     4141 ctgtaggtca ggttgctttc tcaggtatag catgaggtcg ctcttattga ccacacctct
     4201 accggcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     4261 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     4321 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     4381 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     4441 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     4501 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     4561 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     4621 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     4681 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     4741 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     4801 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     4861 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4921 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     4981 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     5041 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     5101 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     5161 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     5221 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     5281 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     5341 gaagttttaa atcaatctaa agtatatatg agtaacctga ggctatggca gggcctgccg
     5401 ccccgacgtt ggctgcgagc cctgggcctt cacccgaact tggggggtgg ggtggggaaa
     5461 aggaagaaac gcgggcgtat tggccccaat ggggtctcgg tggggtatcg acagagtgcc
     5521 agccctggga ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc gttttattct
     5581 gtctttttat tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag
     5641 ttagcctccc cctagggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca
     5701 tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg
     5761 aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag
     5821 tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc
     5881 ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat
     5941 atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc
     6001 gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg
     6061 agtcacgacg agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc
     6121 tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat
     6181 ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg
     6241 atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc
     6301 aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc
     6361 cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga
     6421 tagccgcgct gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa
     6481 aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt
     6541 ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg
     6601 caatccatct tgttcaatca tactcttcct ttttcaatat tattgaagca tttatcaggg
     6661 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     6721 tccgcgcaca tttccccgaa aagtgccacc tgaacgaagc atctgtgctt cattttgtag
     6781 aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta
     6841 cagaacagaa atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt
     6901 tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat
     6961 ttttacagaa cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc
     7021 ttttttgttc tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat
     7081 tacttttttt ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta
     7141 ggtccgttaa ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc
     7201 ctgactccac ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga
     7261 taaaggcatc cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa
     7321 agtgatagcg ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg
     7381 tctctatata ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat
     7441 gaatagttct tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa
     7501 tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata
     7561 gggatatagc acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg
     7621 tattcgcaat attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc
     7681 gtcttcagag cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagagaat
     7741 aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa
     7801 cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt
     7861 atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg cgtacttata
     7921 tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt atcccattcc
     7981 atgcggggta tcgtatgctt ccttcagcac taccctttag ctgttctata tgctgccact
     8041 cctcaattgg attagtctca tccttcaatg ctatcatttc ctttgatatt ggatcatact
     8101 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     8161 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     8221 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     8281 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     8341 tgcaccatag atcaacgaca ttactatata tataatatag gaagcattta atagaacagc
     8401 atcgtaatat atgtgtactt tgcagttatg acgccagatg gcagtagtgg aagatattct
     8461 ttattgaaaa atagcttgtc accttacgta caatcttgat ccggagcttt tctttttttg
     8521 ccgattaaga attaattcgg tcgaaaaaag aaaaggagag ggccaagagg gagggcattg
     8581 gtgactattg agcacgtgag tatacgtgat taagcacaca aaggcagctt ggagtatgtc
     8641 tgttattaat ttcacaggta gttctggtcc attggtgaaa gtttgcggct tgcagagcac
     8701 agaggccgca gaatgtgctc tagattccga tgctgacttg ctgggtatta tatgtgtgcc
     8761 caatagaaag agaacaattg acccggttat tgcaaggaaa atttcaagtc ttgtaaaagc
     8821 atataaaaat agttcaggca ctccgaaata cttggttggc gtgtttcgta atcaacctaa
     8881 ggaggatgtt ttggctctgg tcaatgatta cggcattgat atcgtccaac tgcatggaga
     8941 tgagtcgtgg caagaatacc aagagttcct cggtttgcca gttattaaaa gactcgtatt
     9001 tccaaaagac tgcaacatac tactcagtgc agcttcacag aaacctcatt cgtttattcc
     9061 cttgtttgat tcagaagcag gtgggacagg tgaacttttg gattggaact cgatttctga
     9121 ctgggttgga aggcaagaga gccccgaaag cttacatttt atgttagctg gtggactgac
     9181 gccagaaaat gttggtgatg cgcttagatt aaatggcgtt attggtgttg atgtaagcgg
     9241 aggtgtggag acaaatggtg taaaagactc taacaaaata gcaaatttcg tcaaaaatgc
     9301 taagaaatag gttattactg agtagtattt atttaagtat tgtttgtgca cttgccgatc
     9361 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta
     9421 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     9481 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     9541 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     9601 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     9661 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     9721 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     9781 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     9841 gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc aactgttggg
     9901 aagggcgat
//
