LOCUS       sequence-170829-        9828 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Y2H Gal4 DNA binding domain for Prp5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9828)
  AUTHORS   Carrocci TJ, Zoerner DM, Paulson JC, Hoskins AA
  TITLE     SF3b1 mutations associated with myelodysplastic syndromes alter the 
            fidelity of branchsite selection in yeast.
  JOURNAL   Nucleic Acids Res. 2017 Jan 6. pii: gkw1349. doi: 
            10.1093/nar/gkw1349.
  PUBMED    28062854
REFERENCE   2  (bases 1 to 9828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2017 Jan 6. pii: gkw1349. doi: 10.1093/nar/gkw1349."
COMMENT     Sequence Label: pGBKT7-PRP5
FEATURES             Location/Qualifiers
     source          1..9828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..734
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             762..1202
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     1213..1232
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1213..1231
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1251..1280
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      3863..3910
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      3937..4124
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3992..4016)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(4148..4164)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4148..4164)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4161..4183)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4172..4188
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4196..4202,4203..4220,4221..4226))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4241..4262
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4379..4396)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4550..5138)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4630..4649)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5467..5514
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5539..5558
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5746..6540)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5746..6540)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5857..5876)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6467..6486
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6541..6645)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6672..8014
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(7635..7682)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     8055..8073
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8111..8133)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8233..8252
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8273..8554
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             8555..9229
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(9332..9787)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9469..9490)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9681..9700
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg
       61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
      121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
      181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
      241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
      301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
      361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat gatggaagac
      421 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
      481 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc
      541 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaaagtt
      601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
      661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
      721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta
      781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga
      841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt
      901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc
      961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac
     1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg
     1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa
     1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat
     1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc
     1261 tgatctcaga ggaggacctg catatggaaa ctattgattc gaagcaaaat attaataggg
     1321 agtctttatt ggaggaaagg aggaaaaaac tagcaaaatg gaaacaaaaa aaagcacaat
     1381 ttgatgctca gaaagagcat caaacttcac gcaatgacat tgttactaac agtttagaag
     1441 gtaagcagac tactgaaaag tttacggaaa gacaggaacg tgtaaaagaa gagcttcgga
     1501 agagaaaaaa tgaatttagg aaatctgacg aacctgtatc ggttaagcct tcgaagaaaa
     1561 agtcgaagag aagtaaggta aagaagaaaa tatccttcga cttcagtgat gacgatgatt
     1621 ctgaaatagg agtttctttt agatcgaagg agcacattca aaaagctcca gagcatgata
     1681 atgaaaaaga tccactggat gagtttatga cgtcattgaa ggaggagaaa atgagtaata
     1741 gtaaaggcat gtacgacaga ggcgacattc ttgatgtaga ggatcaattg tttgaacttg
     1801 gaggaaccga cgatgaggat gttgaagata atacggataa ttctaatata gcgaaaattg
     1861 ctaaactcaa agcaaaaaaa cgtgtaaagc aaatttatta ctctccagaa gagcttgagc
     1921 cgttccaaaa aaatttttat atagaatctg aaacagtttc ctcgatgtca gagatggaag
     1981 ttgaggagct tagacttagt ctggataata tcaaaataaa gggaacaggt tgccccaaac
     2041 cagtcacaaa gtggtctcaa ctgggactat caacggacac catggtttta attacagaaa
     2101 agttgcactt tggctcctta acgcctattc aatcccaggc tcttcccgct attatgtcag
     2161 gtcgtgatgt tataggaata tcaaaaactg gctccggcaa gaccatctcc tatcttttac
     2221 cattgctaag acaagtaaaa gctcaacggc cattatcaaa acatgaaaca gggcccatgg
     2281 gtttaattct ggctccaact agagagctag ctttacaaat acatgaagaa gttactaaat
     2341 tcacagaagc agatacatcc attagatcag tatgctgtac aggaggttct gaaatgaaaa
     2401 agcagattac tgatcttaaa agaggcactg agattgttgt tgccacaccg ggacgattta
     2461 ttgatatatt aacactaaat gatgggaaat tacttagtac taaaagaata acgttcgtag
     2521 taatggatga ggcagacagg ctgttcgatt taggttttga acctcaaata acgcaaatca
     2581 tgaaaactgt tcgaccggat aaacagtgtg ttctatttag tgcaactttt ccgaacaaac
     2641 tacgcagttt tgccgtaaga gttttgcatt ccccaatatc tattacgatt aattcaaagg
     2701 gaatggttaa tgaaaacgta aaacaaaagt ttagaatatg ccattcagaa gatgaaaaat
     2761 ttgacaatct ggtgcagctt atccatgagc gcagtgaatt ttttgacgag gttcaaagtg
     2821 aaaatgacgg acagtcaagc gatgttgaag aagttgatgc taaagccatt atatttgtat
     2881 caagtcagaa tatttgcgac ttcatttcaa aaaagctgtt aaatgctgga attgtgacct
     2941 gtgctattca tgcaggtaag ccatatcaag aaaggcttat gaacttagaa aagttcaaac
     3001 gagaaaagaa cagtattctt ctctgtacag aggttctctc aagaggttta aatgttcccg
     3061 aagtgtcgtt ggttattatc tataacgccg tcaaaacttt cgcgcaatat gttcatacta
     3121 ctggaagaac tgccagaggt agtcgctccg gtactgctat tactcttcta ttgcatgatg
     3181 aactgtcagg tgcttatatc ttgagcaagg caatgcgcga tgaagaaata aaagctttag
     3241 accctctgca agcaaaagaa cttcaggaaa tgagtgccaa gtttgaatcc ggtatgaaaa
     3301 agggtaagtt taggttgtcg aaagggttcg gcggcaaagg actggaaaat atcaaaagta
     3361 aaagagagga agctcaaaat aaagatttgg agctcaaaaa gaatgataag aggagcgatg
     3421 acttggaaaa gaaaatcagc aatccacgcg aagggcatga cagtgtgtct gagtcttctg
     3481 cgcttattcc gagactcaat tatgaactat tcaaagagtc tacagacggg tcgatcatct
     3541 tttacgccaa ggtttatatt aatgatttac cccagattgt aagatgggag gctacaaaga
     3601 atacaacctt attatttatc aagcatgaaa ccggatgtag tattactaac aagggtaaat
     3661 tttaccctga agggaaggaa ccaaagaatg aaaacgatga gcctaaacta tacttattga
     3721 tcgaaggcca agatgagaaa gacatacaat taagtataga attgctggag caaaaagtta
     3781 aagagggggt cgtaaaggct gcaagcttgt ctttgaagag tactaaatac tagggatccg
     3841 tcgacctgca gcggccgcat aactagcata accccttggg gcctctaaac gggtcttgag
     3901 gggttttttg cgcgcttgca gccaagctaa ttccgggcga atttcttatg atttatgatt
     3961 tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag
     4021 gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct
     4081 caggtatagc atgaggtcgc tcttattgac cacacctcta ccggcatgca agcttggcgt
     4141 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     4201 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     4261 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     4321 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     4381 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4441 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4501 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4561 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4621 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4681 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4741 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4801 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4861 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4921 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4981 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5041 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5101 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5161 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     5221 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     5281 gtatatatga gtaacctgag gctatggcag ggcctgccgc cccgacgttg gctgcgagcc
     5341 ctgggccttc acccgaactt ggggggtggg gtggggaaaa ggaagaaacg cgggcgtatt
     5401 ggccccaatg gggtctcggt ggggtatcga cagagtgcca gccctgggac cgaaccccgc
     5461 gtttatgaac aaacgaccca acaccgtgcg ttttattctg tctttttatt gccgtcatag
     5521 cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt tagcctcccc ctagggtggg
     5581 cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa
     5641 cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca
     5701 ggttgggcgt cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc
     5761 aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag
     5821 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat
     5881 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc
     5941 attttccacc atgatattcg gcaagcaggc atcgccatga gtcacgacga gatcctcgcc
     6001 gtcgggcatg ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
     6061 ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
     6121 gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
     6181 cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
     6241 ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
     6301 cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg
     6361 cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
     6421 tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
     6481 gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat
     6541 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6601 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6661 agtgccacct gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag
     6721 cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga
     6781 aagcgctatt ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc
     6841 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
     6901 cgcgagagcg ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc
     6961 atcccgagag cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc
     7021 gctctataat gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa
     7081 ggctactttg gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt
     7141 actgattact agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat
     7201 tctataccga tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct
     7261 tcattggtca gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg
     7321 aaatgtttac attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt
     7381 ttttgtctaa agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg
     7441 caagttcaag gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata
     7501 tagcaaagag atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc
     7561 tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt
     7621 ttcaaaagcg ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact
     7681 tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc
     7741 tcactgttca cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga
     7801 acggcatagt gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg
     7861 aaaggtagtc tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc
     7921 cttcagcact accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat
     7981 ccttcaatgc tatcatttcc tttgatattg gatcatacta agaaaccatt attatcatga
     8041 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     8101 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     8161 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     8221 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat
     8281 tactatatat ataatatagg aagcatttaa tagaacagca tcgtaatata tgtgtacttt
     8341 gcagttatga cgccagatgg cagtagtgga agatattctt tattgaaaaa tagcttgtca
     8401 ccttacgtac aatcttgatc cggagctttt ctttttttgc cgattaagaa ttaattcggt
     8461 cgaaaaaaga aaaggagagg gccaagaggg agggcattgg tgactattga gcacgtgagt
     8521 atacgtgatt aagcacacaa aggcagcttg gagtatgtct gttattaatt tcacaggtag
     8581 ttctggtcca ttggtgaaag tttgcggctt gcagagcaca gaggccgcag aatgtgctct
     8641 agattccgat gctgacttgc tgggtattat atgtgtgccc aatagaaaga gaacaattga
     8701 cccggttatt gcaaggaaaa tttcaagtct tgtaaaagca tataaaaata gttcaggcac
     8761 tccgaaatac ttggttggcg tgtttcgtaa tcaacctaag gaggatgttt tggctctggt
     8821 caatgattac ggcattgata tcgtccaact gcatggagat gagtcgtggc aagaatacca
     8881 agagttcctc ggtttgccag ttattaaaag actcgtattt ccaaaagact gcaacatact
     8941 actcagtgca gcttcacaga aacctcattc gtttattccc ttgtttgatt cagaagcagg
     9001 tgggacaggt gaacttttgg attggaactc gatttctgac tgggttggaa ggcaagagag
     9061 ccccgaaagc ttacatttta tgttagctgg tggactgacg ccagaaaatg ttggtgatgc
     9121 gcttagatta aatggcgtta ttggtgttga tgtaagcgga ggtgtggaga caaatggtgt
     9181 aaaagactct aacaaaatag caaatttcgt caaaaatgct aagaaatagg ttattactga
     9241 gtagtattta tttaagtatt gtttgtgcac ttgccgatct atgcggtgtg aaataccgca
     9301 cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat tttgttaaaa
     9361 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     9421 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     9481 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     9541 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     9601 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     9661 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     9721 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     9781 ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgat
//
