LOCUS       sequence-17084-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Crane R, Kloepfer A, Ruderman JV
  TITLE     Requirements for the destruction of human Aurora-A.
  JOURNAL   J Cell Sci 2004 Dec 1;117(Pt 25):5975-83. Epub 2004 Nov 9.
  PUBMED    15536123
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci"; date: "2004-12-1"; volume: "117(Pt 25)"; pages: "5975-83. Epub
            2004 Nov 9"
COMMENT     Sequence Label: hCdh1 (1-125)
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..27
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             37..57
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(453..473)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        458..787
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      638..773
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(797..816)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ggcagcagcc atcatcatca tcatcacagc agcggcgaga atctttattt tcagggccgg
       61 cccatggacc aggactatga gcggcgcctg cttcgccaga tcgtcatcca gaatgagaac
      121 acgatgccac gcgtcacaga gatgcggcgg accctgacgc ctgccagctc cccagtgtcc
      181 tcgcccagca agcacggaga ccgcttcatc ccctccagag ccggagccaa ctggagcgtg
      241 aacttccaca ggattaacga gaatgagaag tctcccagtc agaaccggaa agccaaggac
      301 gccacctcag acaacggcaa agacggcctg gcctactctg ccctgctcaa gaatgagctg
      361 ctgggtgccg gcatcgagaa ggtgcaggac ccgcagactg aggaccgcag actgcagccc
      421 tccacgcctg agaagaagta ggtcgaccct gtggaatgtg tgtcagttag ggtgtggaaa
      481 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
      541 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      601 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
      661 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
      721 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
      781 ttgcaaaaag cttaccatga ccgagtacaa gcccacggtg cg
//
