LOCUS       sequence-170846-        5209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Light Chain Myosin, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-LC-Myosin-N-7
FEATURES             Location/Qualifiers
     source          1..5209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      795..804
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             801..1511
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     polyA_signal    1633..1754
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1670..1689)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1724..1743
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(1761..2216)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1898..1919)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2110..2129
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2243..2347
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2349..2706
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2349..2540
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2372..2392)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2557..2692
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2741..3535
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2741..3535
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2795..2814)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3405..3424
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3723..3742)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3767..3814
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4143..4731
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4632..4651
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4906..5209
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 actcagatct cgagctcaag cttatgtcga gcaagagagc caaggccaag accaccaaga
      301 agaggcccca gagggctacg tccaatgtct tcgccatgtt tgaccagtcc cagatccagg
      361 agtttaagga ggccttcaac atgattgatc agaaccgcga tggcttcatt gataaggagg
      421 acctgcacga catgttggcc tctctgggga agaaccccac agacgagtac ctggagggca
      481 tgatgaacga ggcgccaggg cccatcaact tcaccatgtt cctcacaatg tttggggaga
      541 agctgaacgg cacagacccc gaggatgtga tccgcaatgc ctttgcctgc tttgatgagg
      601 aggcctcagg cttcatccac gaggaccacc tgagggagct gctcaccacc atgggcgacc
      661 gattcacgga tgaggaggtg gacgagatgt accgcgaggc acccattgat aagaagggca
      721 acttcaacta cgtggagttc actcgcatcc tcaaacacgg cgccaaggac aaggacgacc
      781 tggatccacc ggtcgccacc atggtgagca agggcgagga gaccacaatg ggcgtaatca
      841 agcccgacat gaagatcaag ctgaagatgg agggcaacgt gaatggccac gccttcgtga
      901 tcgagggcga gggcgagggc aagccctacg acggcaccaa caccatcaac ctggaggtga
      961 aggagggagc ccccctgccc ttctcctacg acattctgac caccgcgttc gcctacggca
     1021 acagggcctt caccaagtac cccgacgaca tccccaacta cttcaagcag tccttccccg
     1081 agggctactc ttgggagcgc accatgacct tcgaggacaa gggcatcgtg aaggtgaagt
     1141 ccgacatctc catggaggag gactccttca tctacgagat acacctcaag ggcgagaact
     1201 tcccccccaa cggccccgtg atgcagaaga agaccaccgg ctgggacgcc tccaccgaga
     1261 ggatgtacgt gcgcgacggc gtgctgaagg gcgacgtcaa gcacaagctg ctgctggagg
     1321 gcggcggcca ccaccgcgtt gacttcaaga ccatctacag ggccaagaag gcggtgaagc
     1381 tgcccgacta tcactttgtg gaccaccgca tcgagatcct gaaccacgac aaggactaca
     1441 acaaggtgac cgtttacgag agcgccgtgg cccgcaactc caccgacggc atggacgagc
     1501 tgtacaagta agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
     1561 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     1621 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     1681 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     1741 atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     1801 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     1861 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     1921 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     1981 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     2041 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     2101 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     2161 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
     2221 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     2281 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     2341 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
     2401 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     2461 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     2521 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     2581 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     2641 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     2701 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     2761 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     2821 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     2881 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     2941 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     3001 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     3061 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     3121 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     3181 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     3241 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     3301 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     3361 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     3421 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     3481 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     3541 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     3601 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     3661 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta
     3721 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
     3781 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
     3841 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
     3901 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
     3961 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt
     4021 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     4081 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     4141 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     4201 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     4261 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     4321 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     4381 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     4441 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     4501 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     4561 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     4621 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     4681 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     4741 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     4801 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcattagtt attaatagta
     4861 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     4921 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     4981 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     5041 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     5101 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     5161 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatg
//
