LOCUS       sequence-170857-       10715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of KDM3A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10715)
  AUTHORS   Wade MA, Jones D, Wilson L, Stockley J, Coffey K, Robson CN, Gaughan
            L
  TITLE     The histone demethylase enzyme KDM3A is a key estrogen receptor 
            regulator in breast cancer.
  JOURNAL   Nucleic Acids Res. 2015 Jan;43(1):196-207. doi: 10.1093/nar/gku1298.
            Epub 2014 Dec 8.
  PUBMED    25488809
REFERENCE   2  (bases 1 to 10715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku1298"; journalName: "Nucleic Acids Res"; date: 
            "2015-01"; volume: "43"; issue: "1"; pages: "196-207"
COMMENT     Sequence Label: pLenti-V5-KDM3A
FEATURES             Location/Qualifiers
     source          1..10715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    88..109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(124..129,130..147,148..154)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    162..178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     167..189
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     186..202
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     186..202
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     221..241
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        223..241
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        269..495
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             496..676
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    723..848
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1341..1574
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1759..1803
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2082..2385
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2386..2589
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2540..2560
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2586..2610
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2706..2730
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6730..6754)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6807..6848
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6885..6905)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6890..7219
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7070..7205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7267..7314
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7333..7731
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             7818..8051
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8123..8244
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8160..8179)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8214..8233
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8284..8419
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8439..8458)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8440..8458)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8468..8485)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8468..8484)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8477..8499)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8626..9081
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8713..8732)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8923..8944
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9107..9211
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9212..9280,9281..10072)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9430..9449)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
       61 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
      121 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
      181 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
      241 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
      301 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
      361 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
      421 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
      481 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
      541 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
      601 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
      661 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
      721 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
      781 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
      841 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
      901 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
      961 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     1021 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     1081 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     1141 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     1201 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     1261 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     1321 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     1381 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     1441 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     1501 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     1561 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     1621 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     1681 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     1741 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     1801 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     1861 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     1921 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     1981 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     2041 gtgaacggat ctcgacggta tcgataagct tgggagttcc gcgttacata acttacggta
     2101 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     2161 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     2221 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     2281 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     2341 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     2401 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     2461 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     2521 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     2581 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
     2641 ctccatagaa gacaccgact ctagaggatc cactagtcca gtgtggtgga attctgcaga
     2701 tatcaacaag tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc atggtgctca
     2761 cgctcggaga aagttggccg gtattggtgg ggaggaggtt tctcagtctg tccgcagccg
     2821 acggcagcga tggcagccac gacagctggg acgtggagcg cgtcgccgag tggccctggc
     2881 tctccgggac cattcgagct gtttcccaca ccgacgttac caagaaggat ctgaaggtgt
     2941 gtgtggaatt tgatggggaa tcttggagga aaagaagatg gatagaagtc tacagccttc
     3001 taaggagagc atttttagta gaacataatt tggttttagc tgaacgaaag tcacctgaaa
     3061 tttctgaacg aattgtacag tggcctgcaa taacgtacaa acctctgttg gacaaagctg
     3121 gtttgggatc cataacttct gttcgctttc tgggagatca acaaagagta tttctttcta
     3181 aagacctttt gaagcctata caggatgtaa acagtcttcg actttctctt acggataatc
     3241 agattgtcag taaagaattt caagctttga ttgtgaagca tttagatgaa agccatcttt
     3301 taaaaggtga caaaaactta gttggttcag aagtaaaaat ttatagcttg gacccatcta
     3361 ctcagtggtt ttcagcaacc gttgtaaatg gaaacccagc atcaaaaact cttcaagtca
     3421 actgtgagga gattccagca ctgaaaattg ttgatccgtc actgattcat gttgaagttg
     3481 tacacgataa ccttgtgaca tgtggtaatt ctgcaagaat tggagctgta aaacgcaagt
     3541 cttctgagaa taatggaacc ctggtttcca aacaagcaaa atcttgctct gaggcctctc
     3601 ccagtatgtg tcctgtgcag tctgtaccta caacagtttt taaggagata ctgcttggct
     3661 gtactgcagc aactccacct agtaaggacc caagacagca aagtactccc caggctgcca
     3721 actctccacc taaccttgga gcaaaaattc ctcaaggatg tcataaacaa agtttaccag
     3781 aggaaatttc ttcctgtcta aatacaaagt ctgaagctct gagaacaaaa ccagatgtct
     3841 gcaaagcagg gttgctctca aagtcctctc agattggaac tggagacttg aaaattctga
     3901 ctgagccaaa aggcagctgt actcagccta agacaaacac tgatcaggaa aacagattgg
     3961 agtctgttcc acaagcattg actggccttc ctaaggagtg cttacctaca aaggcttctt
     4021 ctaaggcaga attggaaatt gccaatcctc ctgaactgca gaagcaccta gaacatgcac
     4081 cttccccatc ggatgtttca aatgcaccag aagtgaaagc aggtgtcaat agtgatagcc
     4141 ctaataactg ttcaggaaaa aaggtagaac cttcagcttt agcttgccga tcacagaatt
     4201 taaaggaatc ttcagtaaaa gtagataatg aaagctgttg ttcaagaagc aacaataaaa
     4261 tccagaatgc cccatccagg aagtcggttt tgacagaccc agctaaactc aaaaagctgc
     4321 aacagagtgg cgaggccttc gtacaggatg attcttgtgt gaacatcgtg gcacagttgc
     4381 ctaaatgccg agagtgtcgc ttggacagtc tccgcaagga taaggagcaa cagaaggact
     4441 cacctgtgtt ttgccgcttc tttcacttca ggaggttaca attcaacaaa catggtgtgt
     4501 tgcgggtaga aggcttctta acaccaaaca agtatgacaa tgaagcaatt ggcttgtggt
     4561 tacctttaac caaaaacgtt gtggggattg atttggacac agcaaagtac atcttggcca
     4621 acattggaga ccacttctgt caaatggtga tttctgaaaa ggaagctatg tcaactattg
     4681 agccacacag acaggttgct tggaagcgag ctgtcaaagg tgttcgagaa atgtgtgatg
     4741 tgtgcgacac caccatcttc aacctgcact gggtgtgtcc tcggtgtggg tttggagtat
     4801 gtgtggactg ctaccggatg aagagaaaga attgccaaca gggtgctgct tacaagactt
     4861 tctcttggct aaaatgtgtg aagagtcaga tacatgaacc agagaactta atgcccacac
     4921 agatcattcc tggaaaagca ctctatgatg ttggagacat tgttcattct gtaagagcga
     4981 aatggggaat aaaggcaaac tgcccctgct caaacaggca attcaaactc ttttcaaagc
     5041 cagcctcaaa ggaagaccta aaacagactt ctttagctgg agaaaaaccg actcttggtg
     5101 cagtgctcca gcagaatccc tcagtgttgg agccagcagc tgtgggtggg gaagcagcct
     5161 ccaagccagc cggcagcatg aagcctgcct gtccagccag cacatctcct ctaaactggc
     5221 tggccgacct aaccagcggg aatgtcaaca aggaaaacaa ggaaaaacaa ccaacaatgc
     5281 caattttaaa gaatgaaatc aaatgccttc cacccctccc acctttaagc aaatccagca
     5341 cagtcctcca tacgtttaac agcacaattt tgacacccgt aagcaacaac aattctggtt
     5401 tcctccggaa tctcttgaat tcttctacag gaaagacaga aaatggactc aagaatacac
     5461 caaaaatcct tgatgacatc tttgcctctt tggtgcaaaa taagacgact tctgatttat
     5521 ctaagaggcc tcaaggacta accatcaagc ccagcattct gggctttgac actcctcact
     5581 attggctttg tgataatcgc ttgctgtgct tgcaagaccc caacaataag agcaactgga
     5641 atgtgtttag ggagtgctgg aaacaagggc agccagtgat ggtgtctgga gtgcatcata
     5701 aattgaactc tgaactttgg aaacctgaat ccttcaggaa agagtttggt gagcaggaag
     5761 tagacctagt taattgtagg accaatgaaa tcatcacagg agccacagta ggagacttct
     5821 gggatggatt tgaagatgtt ccaaatcgtt tgaaaaatga aaaagaacca atggtgttga
     5881 aacttaagga ctggccacca ggagaagatt ttagagatat gatgccttcc aggtttgatg
     5941 atctgatggc caacattcca ctgcccgagt acacaaggcg agatggcaaa ctgaatttgg
     6001 cctctaggct gccaaactac tttgttcggc cagatctggg ccccaagatg tataatgctt
     6061 atggattaat cactcctgaa gatcggaaat atggaacaac aaatcttcac ttagatgtat
     6121 ctgatgcagc taatgtcatg gtctatgtgg gaattcccaa aggacagtgt gagcaagaag
     6181 aagaagtcct taagaccatc caagatggag attctgacga actcacaata aagcgattta
     6241 ttgaaggaaa agagaagcca ggagcactgt ggcacatata tgctgcaaag gacacggaga
     6301 agataaggga atttcttaaa aaggtatcag aagagcaagg tcaagaaaac ccagcagacc
     6361 acgatcctat tcatgatcaa agctggtatt tagaccgatc attaagaaaa cgtcttcatc
     6421 aagagtatgg agttcaaggc tgggctattg tacagtttct tggggatgtg gtgtttatcc
     6481 cggcaggagc tccacatcag gttcataact tatatagctg catcaaagtg gctgaagatt
     6541 ttgtttctcc agagcatgtt aaacactgct tctggcttac tcaggaattc cgatatctgt
     6601 cacagactca taccaatcac gaagataaat tacaggtgaa gaatgttatc taccatgcag
     6661 tgaaagatgc agttgctatg ctgaaagcca gtgaatccag ttttggcaaa cctaagggtg
     6721 ggcgcgccga cccagctttc ttgtacaaag tggttgatat ccagcacagt ggcggccgct
     6781 cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg
     6841 attctacgcg taccggttag taatgagttt ggaattaatt ctgtggaatg tgtgtcagtt
     6901 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6961 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7021 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     7081 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7141 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     7201 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     7261 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     7321 aggaactaaa ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca
     7381 acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc
     7441 tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt
     7501 gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc
     7561 gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg
     7621 cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg
     7681 gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta agcacaattc
     7741 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7801 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     7861 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7921 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7981 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     8041 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     8101 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     8161 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8221 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     8281 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8341 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     8401 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     8461 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     8521 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     8581 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     8641 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     8701 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     8761 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     8821 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     8881 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     8941 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     9001 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     9061 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     9121 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     9181 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     9241 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     9301 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     9361 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     9421 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     9481 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     9541 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     9601 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     9661 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     9721 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     9781 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     9841 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     9901 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     9961 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
    10021 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
    10081 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
    10141 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
    10201 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
    10261 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
    10321 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
    10381 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
    10441 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
    10501 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
    10561 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    10621 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
    10681 tccggtaagc ggcagggtcg gaacaggaga gcgca
//
