LOCUS       sequence-170871-        5983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression vector containing HIS10-TEV-HA (HTH) epitope tag 
            downstream of the GAL1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Singh RK, Kabbaj MH, Paik J, Gunjan A
  TITLE     Histone levels are regulated by phosphorylation and 
            ubiquitylation-dependent proteolysis.
  JOURNAL   Nat Cell Biol. 2009 Aug;11(8):925-33. doi: 10.1038/ncb1903. Epub 
            2009 Jul 5.
  PUBMED    19578373
REFERENCE   2  (bases 1 to 5983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb1903"; 
            journalName: "Nat Cell Biol"; date: "2009-08"; volume: "11"; issue: 
            "8"; pages: "925-33"
COMMENT     Sequence Label: pYES2-HTH
FEATURES             Location/Qualifiers
     source          1..5983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..442
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    1..118
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     413..436
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     474..493
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        474..492
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             515..544
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             569..589
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             599..625
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      731..978
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(747..765)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(1055..1072)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1226..1814)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1306..1325)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1985..2776,2777..2845))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2608..2627
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(2941..3744)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(3745..3960)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     rep_origin      4561..5438
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     rep_origin      complement(5449..5962)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5644..5665)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5856..5875
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggattagaa gccgccgagc gggtgacagc cctccgaagg aagactctcc tccgtgcgtc
       61 ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa
      121 caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc
      181 tggccccaca aaccttcaaa tgaacgaatc aaattaacaa ccataggatg ataatgcgat
      241 tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatctatt
      301 aacagatata taaatgcaaa aactgcataa ccactttaac taatactttc aacattttcg
      361 gtttgtatta cttcttattc aaatgtaata aaagtatcaa caaaaaattg ttaatatacc
      421 tctatacttt aacgtcaagg agaaaaaacc ccggatcgga ctactagcag ctgtaatacg
      481 actcactata gggaatatta agcttgctag aatgcatcac catcatcacc atcatcacca
      541 ccatgtggat tacgatatcc caactaccga aaacttgtac ttccaaggtg gcggccgcta
      601 cccatacgat gttccagatt acgcccatgg taccgagctc ggatccacta gtaacggccg
      661 ccagtgtgct ggaattctgc agatatccat cacactggcg gccgctcgag catgcatcta
      721 gagggccgca tcatgtaatt agttatgtca cgcttacatt cacgccctcc ccccacatcc
      781 gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt
      841 atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac
      901 agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt tttgggacgc
      961 tcgaaggctt taatttgcaa gctgcggccc tgcattaatg aatcggccaa cgcgcgggga
     1021 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     1081 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1141 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1201 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1261 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1321 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1381 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1441 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1501 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1561 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1621 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1681 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1741 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1801 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1861 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1921 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1981 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2041 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2101 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     2161 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2221 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2281 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2341 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2401 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2461 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2521 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2581 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2641 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2701 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2761 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2821 cgacacggaa atgttgaata ctcatactct tcctttttca atgggtaata actgatataa
     2881 ttaaattgaa gctctaattt gtgagtttag tatacatgca tttacttata atacagtttt
     2941 ttagttttgc tggccgcatc ttctcaaata tgcttcccag cctgcttttc tgtaacgttc
     3001 accctctacc ttagcatccc ttccctttgc aaatagtcct cttccaacaa taataatgtc
     3061 agatcctgta gagaccacat catccacggt tctatactgt tgacccaatg cgtctccctt
     3121 gtcatctaaa cccacaccgg gtgtcataat caaccaatcg taaccttcat ctcttccacc
     3181 catgtctctt tgagcaataa agccgataac aaaatctttg tcgctcttcg caatgtcaac
     3241 agtaccctta gtatattctc cagtagatag ggagcccttg catgacaatt ctgctaacat
     3301 caaaaggcct ctaggttcct ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat
     3361 acctgggccc accacaccgt gtgcattcgt aatgtctgcc cattctgcta ttctgtatac
     3421 acccgcagag tactgcaatt tgactgtatt accaatgtca gcaaattttc tgtcttcgaa
     3481 gagtaaaaaa ttgtacttgg cggataatgc ctttagcggc ttaactgtgc cctccatgga
     3541 aaaatcagtc aagatatcca catgtgtttt tagtaaacaa attttgggac ctaatgcttc
     3601 aactaactcc agtaattcct tggtggtacg aacatccaat gaagcacaca agtttgtttg
     3661 cttttcgtgc atgatattaa atagcttggc agcaacagga ctaggatgag tagcagcacg
     3721 ttccttatat gtagctttcg acatgattta tcttcgtttc ctgcaggttt ttgttctgtg
     3781 cagttgggtt aagaatactg ggcaatttca tgtttcttca acactacata tgcgtatata
     3841 taccaatcta agtctgtgct ccttccttcg ttcttccttc tgttcggaga ttaccgaatc
     3901 aaaaaaattt caaggaaacc gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa
     3961 aagctagctt atcgatgata agctgtcaaa catgagaatt aattccacgg actatagact
     4021 atacctagta tactccgtct actgtacgat acacttccgc tcaggtcctt gtcctttaac
     4081 gaggccttac cactcttttg ttactctatt gatccagctc agcaaaggca gtgtgatcta
     4141 agattctatc ttcgcgatgt agtaaaacta gctagaccga gaaagagact agaaatgcaa
     4201 aaggcacttc tacaatggct gccatcatta ttatccgatg tgacgctgca gcttctcaat
     4261 gatattcgaa tacgctttga ggagatacag cctaatatcc gacaaactgt tttacagatt
     4321 tacgatcgta cttgttaccc atcattgaat tttgaacatc cgaacctggg agttttccct
     4381 gaaacagata gtatatttga acctgtataa taatatatag tctagcgctt tacggaagac
     4441 aatgtatgta tttcggttcc tggagaaact attgcatcta ttgcataggt aatcttgcac
     4501 gtcgcatccc cggttcattt tctgcgtttc catcttgcac ttcaatagca tatctttgtt
     4561 aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt
     4621 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt
     4681 taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa
     4741 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc
     4801 tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc
     4861 gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg
     4921 cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg
     4981 tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta
     5041 gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat
     5101 gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag
     5161 aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca
     5221 ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa
     5281 gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg
     5341 agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga
     5401 tacttttgag caatgtttgt ggaagcggta ttcgcaatgg gaagctccac cccggttgat
     5461 aatcagaaaa gccccaaaaa caggaagatt gtataagcaa atatttaaat tgtaagcgtt
     5521 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     5581 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     5641 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     5701 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     5761 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     5821 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     5881 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     5941 aatgcgccgc tacagggcgc gtggggatcg atccactagt cca
//
