LOCUS       sequence-170875-        6374 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBAc transposon with transformation marker for Tribolium 
            transgenesis; Drives dsRedExpress (red fluorescent) expression in 
            the eyes (much stronger than usual 3XP3); (Transformation plasmid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6374)
  TITLE     Bucher Lab plasmids
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBac[6xP3-Tc-hsp-DsRedExpress-SV40]
FEATURES             Location/Qualifiers
     source          1..6374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1099..1161
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      2364..2373
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2370..3047
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(2500..2518)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(2550..2570)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     2768..2787
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2962..2985
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    3168..3289
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3205..3224)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3259..3278
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   3938..3972
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(4153..4169)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4153..4169)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4166..4188)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4177..4193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4201..4207,4208..4225,4226..4231))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4246..4267
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4384..4401)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4555..5143)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4635..4654)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5314..6105,6106..6174))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5937..5956
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6175..6279)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6347..6365
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctttgttta aaaatataac
      421 aaaattgtga tcccacaaaa tgaagtgggg caaaatcaaa taattaatag tgtccgtaaa
      481 cttgttggtc ttcaactttt tgaggaacac gttggacggc aaatccgtga ctataacaca
      541 agttgattta ataattttag ccaacacgtc gggctgcgtg ttttttgccg acgcgtctgt
      601 gtacacgttg attaactggt cgattaaact gttgaaataa tttaattttt ggttcttctt
      661 taaatctgtg atgaaatttt ttaaaataac tttaaattct tcattggtaa aaaatgccac
      721 gttttgcaac ttgtgagggt ctaatatgag gtcaaactca gtaggagttt tatccaaaaa
      781 agaaaacatg attacgtctg tacacgaacg cgtattaacg cagagtgcaa agtataagag
      841 ggttaaaaaa tatattttac gcaccatata cgcatcgggt tgatatcgtt aatatggatc
      901 aatttgaaca gttgattaac gtgtctctgc tcaagtcttt gatcaaaacg caaatcgacg
      961 aaaatgtgtc ggacaatatc aagtcgatga gcgaaaaact aaaaaggcta gaatacgaca
     1021 atctcacaga cagcgttgag atatacggta ttcacgacag caggctgaat aataaaaaaa
     1081 ttagaaacta ttatttaacc ctagaaagat aatcatattg tgacgtacgt taaagataat
     1141 catgcgtaaa attgacgcat gtgttttatc ggtctgtata tcgaggttta tttattaatt
     1201 tgaatagata ttaagtttta ttatatttac acttacatac taataataaa ttcaacaaac
     1261 aatttattta tgtttattta tttattaaaa aaaaacaaaa actcaaaatt tcttctataa
     1321 agtaacaaaa cttttaaaca ttctctcttt tacaaaaata aacttatttt gtactttaaa
     1381 aacagtcatg ttgtattata aaataagtaa ttagcttaac ttatacataa tagaaacaaa
     1441 ttatacttat tagtcagtca gaaacaactt tggcacatat caatattatg ctctcgacaa
     1501 ataacttttt tgcatttttt gcacgatgca tttgcctttc gccttatttt agaggggcag
     1561 taagtacagt aagtacgttt tttcattact ggctcttcag tactgtcatc tgatgtacca
     1621 ggcacttcat ttggcaaaat attagagata ttatcgcgca aatatctctt caaagtagga
     1681 gcttctaaac gcttacgcat aaacgatgac gtcaggctca tgtaaaggtt tctcataaat
     1741 tttttgcgac tttgaacctt ttctcccttg ctactgacat tatggctgta tataataaaa
     1801 gaatttatgc aggcaatgtt tatcattccg tacaataatg ccataggcca cctattcgtc
     1861 ttcctactgc aggtcatcac agaacacatt tggtctagcg tgtccactcc gcctttagtt
     1921 tgattataat acataaccat ttgcggttta ccggtacttt cgttgataga agcatcctca
     1981 tcacaagatg ataataagta taccatctta gctggcttcg gtttatatga gacgagagta
     2041 aggggtccgt caaaacaaaa catcgatgtt cccactggcc tggagcgact gtttttcagt
     2101 acttccggta tctcgcgttt gtttgatcgc acggttccca caatggtttc gacgatctaa
     2161 ttcaattaga gactaattca attagagcta attcaattaa tccgtttcat atataagcgc
     2221 ggtctcgcgg cgcgttgtca gtgaatttga atcaagcgaa gcgaaagcaa caaagctaag
     2281 caagcgactc aaagcaaatt ttcaaagcga ctttaacaaa gttgagtgaa ttatttacta
     2341 gtgaattcaa agtggtaccg gtcgccacca tggcctcctc cgaggacgtc atcaaggagt
     2401 tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     2461 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     2521 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttccagtac ggctccaagg
     2581 tgtacgtgaa gcaccccgcc gacatccccg actacaagaa gctgtccttc cccgagggct
     2641 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     2701 cctccctgca ggacggctcc ttcatctaca aggtgaagtt catcggcgtg aacttcccct
     2761 ccgacggccc cgtaatgcag aagaagacta tgggctggga ggcctccacc gagcgcctgt
     2821 acccccgcga cggcgtgctg aagggcgaga tccacaaggc cctgaagctg aaggacggcg
     2881 gccactacct ggtggagttc aagtccatct acatggccaa gaagcccgtg cagctgcccg
     2941 gctactacta cgtggactcc aagctggaca tcacctccca caacgaggac tacaccatcg
     3001 tggagcagta cgagcgcgcc gagggccgcc accacctgtt cctgtagcgg ccgcgactct
     3061 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     3121 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     3181 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3241 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa agctgatctg
     3301 acaatgttca gtgcagagac tcggctacgc ctcgtggact ttgaagttga ccaacaatgt
     3361 ttattcttac ctctaatagt cctctgtggc aaggtcaaga ttctgttaga agccaatgaa
     3421 gaacctggtt gttcaataac attttgttcg tctaatattt cactaccgct tgacgttggc
     3481 tgcacttcat gtacctcatc tataaacgct tcttctgtat cgctctggac gtcatcttca
     3541 cttacgtgat ctgatatttc actgtcagaa tcctcaccaa caagctcgtc atcgctttgc
     3601 agaagagcag agaggatatg ctcatcgtct aaagaactac ccattttatt atatattagt
     3661 cacgatatct ataacaagaa aatatatata taataagtta tcacgtaagt agaacatgaa
     3721 ataacaatat aattatcgta tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg
     3781 tcattttgac tcacgcggtc gttatagttc aaaatcagtg acacttaccg cattgacaag
     3841 cacgcctcac gggagctcca agcggcgact gagatgtcct aaatgcacag cgacggattc
     3901 gcgctattta gaaagagaga gcaatatttc aagaatgcat gcgtcaattt tacgcagact
     3961 atctttctag ggttaaaaaa gatttgcgct ttactcgacc taaactttaa acacgtcata
     4021 gaatcttcgt ttgacaaaaa ccacattgtg gccaagctgt gtgacgcgac gcgcgctaaa
     4081 gaatggcaaa ccaagtcgcg cgagcgtcga ctctagagga tccccgggta ccgagctcga
     4141 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     4201 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     4261 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     4321 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     4381 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     4441 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4501 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4561 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4621 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4681 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4741 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4801 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4861 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4921 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4981 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5041 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5101 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5161 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5221 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5281 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5341 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5401 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     5461 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     5521 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     5581 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     5641 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     5701 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     5761 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     5821 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     5881 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     5941 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6001 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6061 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6121 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6181 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6241 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6301 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     6361 gaggcccttt cgtc
//
