LOCUS       sequence-170906-        2859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses C-terminal Halo-tagged human Keap1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Parvez S, Long MJ, Lin HY, Zhao Y, Haegele JA, Pham VN, Lee DK, Aye 
            Y
  TITLE     T-REX on-demand redox targeting in live cells.
  JOURNAL   Nat Protoc. 2016 Dec;11(12):2328-2356. doi: 10.1038/nprot.2016.114. 
            Epub 2016 Oct 27.
  PUBMED    27809314
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2016.114"; journalName: "Nat Protoc"; date: 
            "2016-12"; volume: "11"; issue: "12"; pages: "2328-2356"
COMMENT     Sequence Label: His-Keap1-Tev-Halo
FEATURES             Location/Qualifiers
     source          1..2859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1939..1959
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1966..2856
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgcctcg tggtagccat
       61 atgcagccag atcccaggcc tagcggggct ggggcctgct gccgattcct gcccctgcag
      121 tcacagtgcc ctgagggggc aggggacgcg gtgatgtacg cctccactga gtgcaaggcg
      181 gaggtgacgc cctcccagca tggcaaccgc accttcagct acaccctgga ggatcatacc
      241 aagcaggcct ttggcatcat gaacgagctg cggctcagcc agcagctgtg tgacgtcaca
      301 ctgcaggtca agtaccagga tgcaccggcc gcccagttca tggcccacaa ggtggtgctg
      361 gcctcatcca gccctgtctt caaggccatg ttcaccaacg ggctgcggga gcagggcatg
      421 gaggtggtgt ccattgaggg tatccacccc aaggtcatgg agcgcctcat tgaattcgcc
      481 tacacggcct ccatctccat gggcgagaag tgtgtcctcc acgtcatgaa cggtgctgtc
      541 atgtaccaga tcgacagcgt tgtccgtgcc tgcagtgact tcctggtgca gcagctggac
      601 cccagcaatg ccatcggcat cgccaacttc gctgagcaga ttggctgtgt ggagttgcac
      661 cagcgtgccc gggagtacat ctacatgcat tttggggagg tggccaagca agaggagttc
      721 ttcaacctgt cccactgcca actggtgacc ctcatcagcc gggacgacct gaacgtgcgc
      781 tgcgagtccg aggtcttcca cgcctgcatc aactgggtca agtacgactg cgaacagcga
      841 cggttctacg tccaggcgct gctgcgggcc gtgcgctgcc actcgttgac gccgaacttc
      901 ctgcagatgc agctgcagaa gtgcgagatc ctgcagtccg actcccgctg caaggactac
      961 ctggtcaaga tcttcgagga gctcaccctg cacaagccca cgcaggtgat gccctgccgg
     1021 gcgcccaagg tgggccgcct gatctacacc gcgggcggct acttccgaca gtcgctcagc
     1081 tacctggagg cttacaaccc cagtgacggc acctggctcc ggttggcgga cctgcaggtg
     1141 ccgcggagcg gcctggccgg ctgcgtggtg ggcgggctgt tgtacgccgt gggcggcagg
     1201 aacaactcgc ccgacggcaa caccgactcc agcgccctgg actgttacaa ccccatgacc
     1261 aatcagtggt cgccctgcgc ccccatgagc gtgccccgta accgcatcgg ggtgggggtc
     1321 atcgatggcc acatctatgc cgtcggcggc tcccacggct gcatccacca caacagtgtg
     1381 gagaggtatg agccagagcg ggatgagtgg cacttggtgg ccccaatgct gacacgaagg
     1441 atcggggtgg gcgtggctgt cctcaatcgt ctcctttatg ccgtgggggg ctttgacggg
     1501 acaaaccgcc ttaattcagc tgagtgttac tacccagaga ggaacgagtg gcgaatgatc
     1561 acagcaatga acaccatccg aagcggggca ggcgtctgcg tcctgcacaa ctgtatctat
     1621 gctgctgggg gctatgatgg tcaggaccag ctgaacagcg tggagcgcta cgatgtggaa
     1681 acagagacgt ggactttcgt agcccccatg aagcaccggc gaagtgccct ggggatcact
     1741 gtccaccagg ggagaatcta cgtccttgga ggctatgatg gtcacacgtt cctggacagt
     1801 gtggagtgtt acgacccaga tacagacacc tggagcgagg tgacccgaat gacatcgggc
     1861 cggagtgggg tgggcgtggc tgtcaccatg gagccctgcc ggaagcagat tgaccagcag
     1921 aactgtacct gttccggaga aaacttgtat ttccagggct cagggatggc agaaatcggt
     1981 actggctttc cattcgaccc ccattatgtg gaagtcctgg gcgagcgcat gcactacgtc
     2041 gatgttggtc cgcgcgatgg cacccctgtg ctgttcctgc acggtaaccc gacctcctcc
     2101 tacgtgtggc gcaacatcat cccgcatgtt gcaccgaccc atcgctgcat tgctccagac
     2161 ctgatcggta tgggcaaatc cgacaaacca gacctgggtt atttcttcga cgaccacgtc
     2221 cgcttcatgg atgccttcat cgaagccctg ggtctggaag aggtcgtcct ggtcattcac
     2281 gactggggct ccgctctggg tttccactgg gccaagcgca atccagagcg cgtcaaaggt
     2341 attgcattta tggagttcat ccgccctatc ccgacctggg acgaatggcc agaatttgcc
     2401 cgcgagacct tccaggcctt ccgcaccacc gacgtcggcc gcaagctgat catcgatcag
     2461 aacgttttta tcgagggtac gctgccgatg ggtgtcgtcc gcccgctgac tgaagtcgag
     2521 atggaccatt accgcgagcc gttcctgaac cctgttgacc gcgagccact gtggcgcttc
     2581 ccaaacgagc tgccaatcgc cggtgagcca gcgaacatcg tcgcgctggt cgaagaatac
     2641 atggactggc tgcaccagtc ccctgtcccg aagctgctgt tctggggcac cccaggcgtt
     2701 ctgatcccac cggccgaagc cgctcgcctg gccaaaagcc tgcctaactg caaggctgtg
     2761 gacatcggcc cgggtctgaa tctgctgcaa gaagacaacc cggacctgat cggcagcgag
     2821 atcgcgcgct ggctgtcgac gctcgagatt tccggctga
//
