LOCUS       sequence-170912-        4161 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of photoactivated cyclase bPAC of Beggiatoa sp..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Stierl M, Stumpf P, Udwari D, Gueta R, Hagedorn R, Losi A, Gartner 
            W, Petereit L, Efetova M, Schwarzel M, Oertner TG, Nagel G, Hegemann
            P
  TITLE     Light modulation of cellular cAMP by a small bacterial 
            photoactivated adenylyl cyclase, bPAC, of the soil bacterium 
            Beggiatoa.
  JOURNAL   J Biol Chem. 2011 Jan 14. 286(2):1181-8.
  PUBMED    21030594
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2011 Jan 14. 286(2):1181-8."
COMMENT     Sequence Label: bPAC-mycHis_pGEM
FEATURES             Location/Qualifiers
     source          1..4161
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(70..88)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(71..88)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(106..122)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(106..122)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(119..141)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    130..146
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(154..160,161..178,179..184))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    199..220
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(337..354)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(508..1096)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(588..607)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1267..2058,2059..2127))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1890..1909
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2128..2232)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2300..2318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2356..2378)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2478..2497
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2691..2713
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2705..2722
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2706..2722
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2729..2748
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2729..2747
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           2779..2821
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             3918..3947
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3948..3977
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     3'UTR           4026..4151
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
ORIGIN
        1 cccccccctg caggcatgcg tttaaacatt taaattacgt attaattaag atatcgctag
       61 cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc
      121 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      181 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      241 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
      301 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      361 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      421 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      481 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      541 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      601 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      661 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      721 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      781 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      841 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      901 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
      961 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1021 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1081 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1141 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1201 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1261 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1321 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1381 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     1441 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1501 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1561 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1621 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     1681 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     1741 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     1801 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     1861 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     1921 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     1981 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     2041 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     2101 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     2161 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     2221 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     2281 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     2341 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     2401 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     2461 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     2521 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
     2581 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     2641 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     2701 acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg aattaattcg
     2761 agctcggtac ccagcttgct tgttcttttt gcagaagctc agaataaacg ctcaactttg
     2821 gcagatcaat tccccgggga tccaagcttg ccaccatgat gaagcggctg gtgtacatca
     2881 gcaagatcag cggccacctg agcctggaag agatccagcg gatcggcaag gtgtccatca
     2941 agaacaacca gcgggacaac atcaccggcg tgctgctgta cctgcagggc ctgttcttcc
     3001 agatcctgga aggcgagaac gagaaggtgg acaagctgta caagaaaatc ctggtggacg
     3061 accggcacac caacatcctg tgcctgaaaa ccgagtacga catcaccgac cggatgttcc
     3121 ccaactgggc catgaaaacc atcaacctga acgagaacag cgagctgatg atccagccca
     3181 tcaagagcct gctgcagacc atcacccaga gccaccgggt gctggaaaag tacatgcccg
     3241 ccagagtgat ctacctgatc aaccagggca tcaaccccct gaccgtggag ccccagctgg
     3301 tcgagaagat catcttcttc agcgacatcc tggccttcag caccctgacc gagaagctgc
     3361 ccgtgaacga ggtggtcatc ctggtcaacc ggtacttcag catctgcacc cggatcatca
     3421 gcgcctacgg cggcgaagtg accaagttca tcggcgactg cgtgatggcc agcttcacca
     3481 aagagcaggg cgacgccgcc atccggacca gcctggacat catctccgag ctgaagcagc
     3541 tgcggcacca cgtggaggcc accaaccccc tgcacctgct gtacaccggc atcggcctga
     3601 gctacggcca cgtgatcgag ggcaacatgg gcagcagcct gaagatggac cacaccctgc
     3661 tgggggacgc cgtgaacgtg gccgccaggc tggaagccct gacaagacag ctgccctacg
     3721 ccctggcctt tacagccggc gtgaagaagt gctgccaggc ccagtggacc ttcatcaacc
     3781 tgggagccca ccaggtcaag ggcaagcagg aagccatcga ggtctacacc gtgaatgagg
     3841 cccagaagta ctacgacacc ctgcagatca cccagctgat ccggcagacc ctggaaaacg
     3901 acaagccgcg gacgtacgag caaaagctga tctcggagga ggacctgcac catcaccatc
     3961 accatcacca tcaccatctc gagtaagcgg ccgcaggacc tgtaagcttg atctggttac
     4021 cactaaacca gcctcaagaa cacccgaatg gagtctctaa gctacataat accaacttac
     4081 actttacaaa atgttgtccc ccaaaatgta gccattcgta tctgctccta ataaaaagaa
     4141 agtttcttca cattctaaaa a
//
