LOCUS       sequence-170913-        4862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella
            emersonii enables fast optical control of cGMP signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Scheib U, Stehfest K, Gee CE, Korschen HG, Fudim R, Oertner TG, 
            Hegemann P
  TITLE     The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella
            emersonii enables fast optical control of cGMP signaling.
  JOURNAL   Sci Signal. 2015 Aug 11;8(389):rs8. doi: 10.1126/scisignal.aab0611.
  PUBMED    26268609
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2015-08-11"; volume: "8(389):rs8. doi"; pages: " 
            10.1126/scisignal.aab0611"
COMMENT     Sequence Label: RhGC(BE)_pGEM
FEATURES             Location/Qualifiers
     source          1..4862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(68..86)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(69..86)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(104..120)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(104..120)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(117..139)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    128..144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(152..158,159..176,177..182))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    197..218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(335..352)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(506..1094)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(586..605)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1265..2056,2057..2125))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1888..1907
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2126..2230)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2298..2316
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2354..2376)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2476..2495
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2689..2711
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2703..2720
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2704..2720
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2727..2746
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2727..2745
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           2777..2819
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
ORIGIN
        1 cccccctgca ggcatgcgtt taaacattta aattacgtat taattaagat atcgctagct
       61 tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg
      121 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
      181 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      241 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      301 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
      361 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      421 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      481 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      541 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      601 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      661 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      721 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      781 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      841 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      901 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      961 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1021 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1081 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1141 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1201 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1261 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1321 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1381 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1441 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1501 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1561 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1621 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1681 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1741 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1801 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1861 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1921 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1981 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2041 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2101 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2161 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2221 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     2281 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     2341 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     2401 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     2461 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     2521 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     2581 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     2641 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     2701 gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa ttaattcgag
     2761 ctcggtaccc agcttgcttg ttctttttgc agaagctcag aataaacgct caactttggc
     2821 agatcaattc cccggggatc catgaaagat aaggataata acctgagagg agcttgttct
     2881 agctgtaact gccctgaata ctgttttagc ccaacttcca ccctgtgcga cgattgcaag
     2941 tgttccgtga ccaaacaccc aatcgtcgaa cagcccctgt cccgaaacgg atctttccgg
     3001 agctccggag caagtctgct gccatcacct tctagtccca atgtgaagat tacctctaca
     3061 gtcggcctgc ggagtagaaa aagcgagtcc caggcaaacg tgcgcgggtc tatgatctct
     3121 aacagtaatt caggaagccg atccaacaat agtggaggag caggaggagg gtcaggaggc
     3181 tcaagctcct ctaagggggg cagcgccctg gctaactacc agtccgctat gtctgagctg
     3241 tggtcttgga atatgatgct gagtactcct tcactgaaat tcctgaccgt gcagtttacc
     3301 acatggattg tgctgactac cgtcggcgcc atctacactc tgttctttca tgaaagacag
     3361 gcttataata gggggtgggc agacatctgg tacggctatg gggctttcgg atttggcctg
     3421 gggctgagct tcgcatatat gggcttcacc ggcgccagaa acccagagaa gaaagctctg
     3481 agcctgtgcc tgctgggggt gaatttcatt agctttatgt cctacatcat catcatgctg
     3541 cggctgactc ccaccatcga aggcaccatg gccaacccag tggagcccgc tagatatctg
     3601 gaatggatcg ccacttgccc tgtcctgatc ctgctgatta gcgagatcac ccagtaccct
     3661 cacgacccat ataaagtgat cgtcaatgat tacgccctgt gcctggcagg attcgtggga
     3721 gcaatcagcg cccagcagcc atggggcgac ctggcacatt tcgtgagctg cctgtgcttt
     3781 tcctacgtgg tctattctct gtggagttgc ttcacagggg ctatcgatgg agagactcag
     3841 tgtaacgtgg aaaaaagcgg cctgaggtgg attcgctttt caacaatcac aacttggagc
     3901 ctgttcccca ttacctggtt ttcatataca agcggcctga tctctttcac tgtggcagag
     3961 gccgggttta gtatgattga catcggagcc aaggtcttcc tgaccctggt gctggtcaac
     4021 agcacagtgg aacaggcaca gaatcagaaa gtcgacgcca ttaccgctat cgcagaggaa
     4081 ctggagaacc agattaacaa ttgtgatgcc atcctgcaga agatgatgcc cgagggcgtg
     4141 ctggaacagc tgaagaatgg gcaggctact gaggcaaaag agtacgaatc cgtgaccgtc
     4201 ttcttttctg atattactaa ctttaccgtg atcagttcaa ggacatccac taaggacatg
     4261 atggccaccc tgaacaagct gtggctggaa tatgatgcca ttgctaagcg gtggggagtg
     4321 tataaagtcg agacaatcgg cgacgcttat ctgggagtga ctggagcacc agacgtggtc
     4381 cctgatcacg cagagcgagc ttgcaacttc gccgtggata tcatcgaaat gattaagagc
     4441 tttaaaacca tcacaggcga gtccattaac atccgcattg gcctgaatag cggacctgtg
     4501 accgcaggag tcctgggcga cctgaaccca cactggtgtc tggtgggaga tacagtcaat
     4561 actgcctccc ggatggaatc tacaagtaaa gctggccaca tccatatttc agagagcacc
     4621 taccatttca tcaagtctaa atttgtgaca cagcctctgg atgtgatgga ggtgaaaggc
     4681 aaaggcaaaa tgcagactta ttgggtgctg ggcagaaaat agaagcttga tctggttacc
     4741 actaaaccag cctcaagaac acccgaatgg agtctctaag ctacataata ccaacttaca
     4801 ctttacaaaa tgttgtcccc caaaatgtag ccattcgtat ctgctcctaa taaaaagaaa
     4861 gt
//
