LOCUS       sequence-170919-       11430 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For creating drosophila transgenics expressing 
            LexAop2->Bxb1.STOP>myr::4xSNAPf.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11430)
  AUTHORS   Sutcliffe B, Ng J, Auer TO, Pasche M, Benton R, Jefferis GS, Cachero
            S
  TITLE     Second-Generation Drosophila Chemical Tags: Sensitivity, 
            Versatility, and Speed.
  JOURNAL   Genetics. 2017 Apr;205(4):1399-1408. doi: 
            10.1534/genetics.116.199281. Epub 2017 Feb 16.
  PUBMED    28209589
REFERENCE   2  (bases 1 to 11430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.116.199281"; journalName: "Genetics"; date: 
            "2017-04"; volume: "205"; issue: "4"; pages: "1399-1408"
COMMENT     Sequence Label: LexAop2->Bxb1.STOP>myr::4xSNAPf
FEATURES             Location/Qualifiers
     source          1..11430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(136..204,205..996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(354..373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1167..1755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1656..1675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1842..1911
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2136..2154)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2606..2677,3079..3352,3427..4081,4143..4458,4662..4793,
                     4864..5478)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     promoter        6890..7128
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     7075..7096
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          7143..7209
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     protein_bind    7490..7537
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             7542..7631
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     primer_bind     complement(7655..7671)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7656..7672)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     intron          7763..7828
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7958..7978
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8250..8384
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8287..8306)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8341..8360
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    8386..8423
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             8427..8972
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             8991..9536
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             9555..10100
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             10122..10667
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     primer_bind     complement(11376..11394)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
       61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1801 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
     1861 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
     1921 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
     1981 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
     2041 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
     2101 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt
     2161 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta
     2221 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat
     2281 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc
     2341 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt
     2401 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa
     2461 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct
     2521 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca
     2581 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg
     2641 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat
     2701 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag
     2761 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag
     2821 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag
     2881 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat
     2941 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga
     3001 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga
     3061 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc
     3121 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt
     3181 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt
     3241 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta
     3301 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt
     3361 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa
     3421 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg
     3481 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat
     3541 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca
     3601 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc
     3661 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc
     3721 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg
     3781 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg
     3841 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct
     3901 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca
     3961 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga
     4021 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct
     4081 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc
     4141 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg
     4201 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat
     4261 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag
     4321 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc
     4381 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg
     4441 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat
     4501 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt
     4561 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact
     4621 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg
     4681 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca
     4741 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc
     4801 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt
     4861 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc
     4921 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca
     4981 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc
     5041 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta
     5101 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata
     5161 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa
     5221 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg
     5281 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg
     5341 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg
     5401 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg
     5461 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc
     5521 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
     5581 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
     5641 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
     5701 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
     5761 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
     5821 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
     5881 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca
     5941 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat
     6001 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt
     6061 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt
     6121 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca
     6181 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg
     6241 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact
     6301 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
     6361 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
     6421 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca
     6481 gggccgcaag cttgcatgcc tgcaggttac tgtacatcca tacagtaagt actgtacatc
     6541 catacagtaa gtactgtaca tccatacagt aagtactgta catccataca gtaagtactg
     6601 tacatccata cagtaagcgg agactctagc cctagggcat gcctgcaggt tactgtacat
     6661 ccatacagta agtactgtac atccatacag taagtactgt acatccatac agtaagcgga
     6721 gactctagcg ctagcgcatg cctgcaggtt actgtacatc catacagtaa gtactgtaca
     6781 tccatacagt aagtactgta catccataca gtaagtactg tacatccata cagtaagtac
     6841 tgtacatcca tacagtaagc ggagactcta gcactagtga cgtcgagcgc cggagtataa
     6901 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg
     6961 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa
     7021 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact
     7081 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagatctaaa
     7141 aggtaggttc aaccactgat gcctaggcac accgaaacga ctaaccctaa ttcttatcct
     7201 ttacttcagg caattacaaa ttatataaca aaatgggcaa caaatgctgc agcaagcgac
     7261 aggatcagga actggcactg gcctatccca ctgggggcta caagaaatcc gactacacct
     7321 ttggccagac gcacatcaac agcagcggcg gcggcaacat gggcggcgtt cttggccaga
     7381 agcataacaa cggtggctcg ctggactcgc gctacacgcc cgatcccaat catcggggtc
     7441 cgttgaaaat cggcggaaag ggcggcgttg acatcatcag accacgcgtg gtttgtctgg
     7501 tcaaccaccg cggtctcagt ggtgtacggt acaaacccag ttacccatac gatgttcctg
     7561 actatgcggg ctatccctat gacgtcccgg actatgcagg atcctatcca tatgacgttc
     7621 cagattacgc ttaagaattc gatatcaagc ttatcgatac cgtcgacctc gagggtacct
     7681 ctagaggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     7741 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     7801 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     7861 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
     7921 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
     7981 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
     8041 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
     8101 gaaaattatg gaaaaatatt tgatgtatag tgccttgact agagatcata atcagccata
     8161 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     8221 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     8281 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     8341 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccggctt gtcgacgacg
     8401 gcggtctccg tcgtcaggat catcttatgg acaaagactg cgaaatgaag cgcaccaccc
     8461 tggatagccc tctgggcaag ctggaactgt ctgggtgcga acagggcctg caccgtatca
     8521 tcttcctggg caaaggaaca tctgccgccg acgccgtgga agtgcctgcc ccagccgccg
     8581 tgctgggcgg accagagcca ctgatgcagg ccaccgcctg gctcaacgcc tactttcacc
     8641 agcctgaggc catcgaggag ttccctgtgc cagccctgca ccacccagtg ttccagcagg
     8701 agagctttac ccgccaggtg ctgtggaaac tgctgaaagt ggtgaagttc ggagaggtca
     8761 tcagctacag ccacctcgct gccctcgccg gcaatccagc tgctacagcc gccgttaaaa
     8821 ccgccctgag cggaaatccc gtgcccattc tgatcccctg ccaccgggtg gtgcagggcg
     8881 acctggacgt gggaggctac gagggcgggc tcgccgtgaa agagtggctg ctggcccacg
     8941 agggccacag actgggcaag cctgggctgg gtagcgtaag cggtagtaca atggacaaag
     9001 actgcgaaat gaagcgcacc accctggata gccctctggg caagctggaa ctgtctgggt
     9061 gcgaacaggg cctgcaccgt atcatcttcc tgggcaaagg aacatctgcc gccgacgccg
     9121 tggaagtgcc tgccccagcc gccgtgctgg gcggaccaga gccactgatg caggccaccg
     9181 cctggctcaa cgcctacttt caccagcctg aggccatcga ggagttccct gtgccagccc
     9241 tgcaccaccc agtgttccag caggagagct ttacccgcca ggtgctgtgg aaactgctga
     9301 aagtggtgaa gttcggagag gtcatcagct acagccacct cgctgccctc gccggcaatc
     9361 cagctgctac agccgccgtt aaaaccgccc tgagcggaaa tcccgtgccc attctgatcc
     9421 cctgccaccg ggtggtgcag ggcgacctgg acgtgggagg ctacgagggc gggctcgccg
     9481 tgaaagagtg gctgctggcc cacgagggcc acagactggg caagcctggg ctgggtgtaa
     9541 gcggtagcag taccatggac aaagactgcg aaatgaagcg caccaccctg gatagccctc
     9601 tgggcaagct ggaactgtct gggtgcgaac agggcctgca ccgtatcatc ttcctgggca
     9661 aaggaacatc tgccgccgac gccgtggaag tgcctgcccc agccgccgtg ctgggcggac
     9721 cagagccact gatgcaggcc accgcctggc tcaacgccta ctttcaccag cctgaggcca
     9781 tcgaggagtt ccctgtgcca gccctgcacc acccagtgtt ccagcaggag agctttaccc
     9841 gccaggtgct gtggaaactg ctgaaagtgg tgaagttcgg agaggtcatc agctacagcc
     9901 acctcgctgc cctcgccggc aatccagctg ctacagccgc cgttaaaacc gccctgagcg
     9961 gaaatcccgt gcccattctg atcccctgcc accgggtggt gcagggcgac ctggacgtgg
    10021 gaggctacga gggcgggctc gccgtgaaag agtggctgct ggcccacgag ggccacagac
    10081 tgggcaagcc tgggctgggt tcggttggta gtagtagcac gatggacaaa gactgcgaaa
    10141 tgaagcgcac caccctggat agccctctgg gcaagctgga actgtctggg tgcgaacagg
    10201 gcctgcaccg tatcatcttc ctgggcaaag gaacatctgc cgccgacgcc gtggaagtgc
    10261 ctgccccagc cgccgtgctg ggcggaccag agccactgat gcaggccacc gcctggctca
    10321 acgcctactt tcaccagcct gaggccatcg aggagttccc tgtgccagcc ctgcaccacc
    10381 cagtgttcca gcaggagagc tttacccgcc aggtgctgtg gaaactgctg aaagtggtga
    10441 agttcggaga ggtcatcagc tacagccacc tcgctgccct cgccggcaat ccagctgcta
    10501 cagccgccgt taaaaccgcc ctgagcggaa atcccgtgcc cattctgatc ccctgccacc
    10561 gggtggtgca gggcgacctg gacgtgggag gctacgaggg cgggctcgcc gtgaaagagt
    10621 ggctgctggc ccacgagggc cacagactgg gcaagcctgg gctgggttag tgactcgaga
    10681 ctagttaatc tagaatgaat cgtttttaaa ataacaaatc aattgtttta taatattcgt
    10741 acgattcttt gattatgtaa taaaatgtga tcattaggaa gattacgaaa aatataaaaa
    10801 atatgagttc tgtgtgtata acaaatgctg taaacgccac aattgtgttt gttgcaaata
    10861 aacccatgat tatttgatta aaattgttgt tttctttgtt catagacaat agtgtgtttt
    10921 gcctaaacgt gtactgcata aactccatgc gagtgtatag cgagctagtg gctaacgctt
    10981 gccccaccaa agtagattcg tcaaaatcct caatttcatc accctcctcc aagtttaaca
    11041 tttggccgtc ggaattaact tctaaagatg ccacataatc taataaatga aatagagatt
    11101 caaacgtggc gtcatcgtcc gtttcgacca tttccgaaaa gaactcgggc ataaactcta
    11161 tgatttctct ggacgtggtg ttgtcgaaac tctcaaagta cgcagtcagg aacgtgcgcg
    11221 acatgtcgtc gggaaactcg cgcggaaaca tgttgttgta accgaacggg tcccatagcg
    11281 ccaaaaccaa atctgccagc gtcaatagaa tgagcacgat gccgacaatg gagctggctt
    11341 ggatagcgat tcgagttaac cttgaagacg aaagggcctc gtgatacgcc tatttttata
    11401 ggttaatgtc atgataataa tggtttctta
//
