LOCUS       sequence-170931-        4819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cGAL driver (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR) construct 
            for C. elegans using the S. kudriavzevii (SK) GAL4 DNA-binding 
            domain (DBD).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Wang H, Liu J, Gharib S, Chai CM, Schwarz EM, Pokala N, Sternberg PW
  TITLE     cGAL, a temperature-robust GAL4-UAS system for Caenorhabditis 
            elegans.
  JOURNAL   Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4109.
  PUBMED    27992408
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Dec 19. doi: 10.1038/nmeth.4109."
COMMENT     Sequence Label: pHW393 (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'...
FEATURES             Location/Qualifiers
     source          1..4819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1813..1962
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        2500..2604
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2605..2673,2674..3465)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2823..2842)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3636..4224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4125..4144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4378..4395
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4512..4533
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4548..4553,4554..4571,4572..4578)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4586..4602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4591..4613
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4610..4626
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4610..4626
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tatttgatat tcttaaaatg tactctataa aagatgaaac tgtaatgacc aattccctac
       61 ataaatattt ttaactaatt ggtcggatat ttggggatca gagaagtact tgtttttgcg
      121 atattaataa tgatatattt ttctagtaaa aaaatgttaa gcgatttgtg aataattaag
      181 ttggcttgaa aaagattttt tgatagctat gtattttgtt aatttaaatc agattttaaa
      241 actttattat tgactattta tatatattat atattatata ttttcatggt ttaatcaaaa
      301 tctattaaat tatttctaaa atctctaaat atcgaattct ctaaaaagct tcatcaacaa
      361 aagcatttca attatctaac acaatgagaa taattcaaga actgaaatag aaagaaatct
      421 agaaaaaaaa gagagtgagg tgccctatta aatcggtgct ctcctctcta acagaagcgc
      481 ctacacgcac ccaagagagc tctttttctt tcttttttcg gagcacacat acacgcatac
      541 actcctcaga agtgtataga gaaaatggga gaagaagcga aagaagacga agaagcggtt
      601 gtgatgtatg catcgggcgt ttctatctct ctccgtgagc aacgagctag tcaacccatc
      661 gatccatttt tgtgagcaca cacagagaga gactcaaaca cactcacagc ttcacactga
      721 gctccaagag ctctgtagag agaagccaga gtatgctttc ctcgattttc ctaacgtctt
      781 cttcttctgc ttttttcttt ttcccaacga gttgtagtcc actaacttca atttttcctc
      841 cagagaaaga gttttagaga gacagctgat aaatacacta gagaaataaa aagaatgata
      901 aaaatttgct attttaatca gttccctctc gtttctctaa gttttgttgt tgggttggga
      961 aattaaaatt ggaaaatgga aataacgttt ttttctgtct aatcttgaat tctccagaca
     1021 tttcttattt ccttttttgt tgcaagtttt tcttccttct ctaattttta atcaaagata
     1081 tctcgctatg aactttgaat ttttgatatt ttgattctat aaatacataa agtaatctaa
     1141 atgctctttt aaaataaatc tacagtagcc ctattttcag ggcgcgcctc tagaggatcc
     1201 ccgggattgg ccaaaggacc caaaggtatg tttcgaatga tactaacata acatagaaca
     1261 ttttcaggag gacccttggc taggatgggc cctaaaaaga agcgtaaagc tagcatgaag
     1321 ctgttgtctt caatggaaca agcttgcgac atatgccggt taaaaaaact caggtgctcg
     1381 aaggaaaagc ccaagtgtgc caagtgtctg aagaacaact cggagtgccg ttattctccc
     1441 aaggctatga ggtccccact gaccagggcg catctgacag aggtagaatc aaggttggaa
     1501 aaattggaag atttgtttct gctcatgttt cctcgggaaa acctggacag cattttgaac
     1561 atggactctt tggacgaagt gaaagtaatg ttgaaacaat tatatatgca ggactatggt
     1621 gacacggatg ctgctatgga tagcttgacc cctatggaaa atggcatgtc tgaagggccg
     1681 agaaaacaca tgaagactgc gacatcatcg ccaaaagtgg atgaagatgg aagccaaagt
     1741 cagttgagtg tatcgccgga attcccgctg cagtctggtg gcggaggggc tcctaggggt
     1801 tccggacggg ctgacgcatt ggacgatttt gatctggata tgctgggaag tgacgccctc
     1861 gatgattttg accttgacat gcttggttcg gatgcccttg atgactttga cctcgacatg
     1921 ctcggcagtg acgcccttga tgatttcgac ctggacatgc tgattaacta aggtaccgaa
     1981 ttcgctagcc ggccatacaa gtaatccgga tgatcgacgc caacgtcgtt gaattttcaa
     2041 attttaaata ctgaatattt gttttttttc ctattattta tttattctct ttgtgttttt
     2101 tttcttgctt tctaaaaaat taattcaatc caaatctaaa catttttttt tctctttccg
     2161 tctcccaatt cgtattccgc tcctctcatc tgaacacaat gtgcaagttt atttatcttc
     2221 tcgctttcat ttcattagga cgtgggggga attggtggaa gggggaaaca cacaaaagga
     2281 tgatggaaat gaaataagga cacacaatat gcaacaacat tcaattcaga aatatggagg
     2341 aaggtttaaa agaaaacata aaaatatata gaggaggaag gaaaactagt aaaaaataag
     2401 caaagaaatt aggcgaacga tgagaattgt cctcgcttgg gcccaaaagg cctttttttt
     2461 ttggcgccga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     2521 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     2581 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     2641 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     2701 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     2761 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     2821 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     2881 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     2941 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3001 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3061 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3121 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3181 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3241 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3301 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     3361 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3421 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     3481 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     3541 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     3601 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3661 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3721 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3781 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3841 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3901 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3961 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4021 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4081 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4141 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4201 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     4261 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     4321 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     4381 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     4441 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     4501 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     4561 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     4621 tatgaccatg attacgccaa gctgtaagtt taaacatgat cttactaact aactattctc
     4681 atttaaattt tcagagctta aaaatggctg aaatcactca caacgatgga tacgctaaca
     4741 acttggaaat gaaataagct tgcatgcgcg gccgcactga ctgggccggc cgatcttcag
     4801 atgggagcag tggactgtc
//
