LOCUS       sequence-170955-       11058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral vector, conditional expression membrane Tdtomato and 
            Kif5Cdelta560.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11058)
  AUTHORS   Lim L, Pakan JMP, Selten MM, Marques-Smith A, Llorca A, Bae SE, 
            Rochefort NL, Marin O
  TITLE     Optimization of interneuron function by direct coupling of cell 
            migration and axonal targeting.
  JOURNAL   Nat Neurosci. 2018 Jun 18. pii: 10.1038/s41593-018-0162-9. doi: 
            10.1038/s41593-018-0162-9.
  PUBMED    29915195
REFERENCE   2  (bases 1 to 11058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2018 Jun 18. pii: 10.1038/s41593-018-0162-9. doi: 
            10.1038/s41593-018-0162-9."
COMMENT     Sequence Label: RV-cag-Dio-Kif5c-2A-tdt
FEATURES             Location/Qualifiers
     source          1..11058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        135..514
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        515..718
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             719..894
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    957..1156
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1357..1773
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1703..1725
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1741..1757
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1787..2166
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2169..2444
                     /label=chicken beta-actin promoter
     intron          2445..3453
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3377..3399
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     protein_bind    complement(3523..3556)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(3607..3640)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(3649..5079)
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             complement(5149..5205)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(join(5239..5949,5950..5952,5953..5955))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5281..5302)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5630..5649
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5889..5910
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5952..5961
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(6761..6772)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    7662..7695
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    7746..7779
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7789..8377
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7842..7862)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8260..8271)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8450..9043
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(9244..9261)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9415..10003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9495..9514)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10181..10972,10973..11041))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10804..10823
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gatccggcca ttagccatat tattcattgg ttatatagca taaatcaata ttggctattg
       61 gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct catgtccaac
      121 attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
      181 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      241 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      301 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      361 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      421 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      481 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      541 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      601 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      661 cccattgacg caaatgggcg gtaggcatgt acggtgggag gtctatataa gcagagctca
      721 ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg
      781 ggtacccgta ttcccaataa agcctcttgc tgtttgcatc cgaatcgtgg tctcgctgtt
      841 ccttgggagg gtctcctctg agtgattgac tacccacgac gggggtcttt catttggggg
      901 ctcgtccggg atttggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
      961 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgtttga tgttatgcgc
     1021 ctgcgtctgt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
     1081 gagttctgaa cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt
     1141 tgtggcccga cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt
     1201 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
     1261 tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc
     1321 tgtctgactg tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta
     1381 agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat
     1441 gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga
     1501 tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt
     1561 tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg
     1621 gcttttgacc cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt
     1681 cctccatccg ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc
     1741 ctttatccag ccctcactcc ttctctaggc gccggaattc gatgtcgaca ttgattattg
     1801 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     1861 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     1921 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     1981 caatgggtgg actatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2041 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2101 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2161 accatgggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     2221 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     2281 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
     2341 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
     2401 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgc
     2461 cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc gccccggctc tgactgaccg
     2521 cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct
     2581 tggtttaatg acggctcgtt tcttttctgt ggctgcgtga aagccttaaa gggctccggg
     2641 agggcccttt gtgcgggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg
     2701 agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc
     2761 tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg
     2821 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
     2881 ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac ccccctcccc gagttgctga
     2941 gcacggcccg gcttcgggtg cggggctccg tgcggggcgt ggcgcggggc tcgccgtgcc
     3001 gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga
     3061 gggctcgggg gaggggcgcg gcggccccgg agcgccggcg gctgtcgagg cgcggcgagc
     3121 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
     3181 atctggcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcgggcgaa
     3241 gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg
     3301 ccgtcccctt ctccatctcc agcctcgggg ctgccgcagg gggacggctg ccttcggggg
     3361 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
     3421 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttgttgtg
     3481 ctgtctcatc attttggcaa agaattcgct agcggatccg gaataacttc gtataggata
     3541 ctttatacga agttatgcag aatggtagct ggattgtagc tgctattagc aatatgaaac
     3601 ctcttaataa cttcgtatag catacattat acgaagttat ttaattaatt acttgtacag
     3661 ctcgtccatg ccgtacagga acaggtggtg gcggccctcg gagcgctcgt actgttccac
     3721 gatggtgtag tcctcgttgt gggaggtgat gtccagcttg gtgtccacgt agtagtagcc
     3781 gggcagttgc acgggcttct tggccatgta gatggtcttg aactccacca ggtagtggcc
     3841 gccgtccttc agcttcaggg cctggtggat ctcgcccttc agcacgccgt cgcgggggta
     3901 caggcgctcg gtggaggcct cccagcccat ggtcttcttc tgcattacgg ggccgtcggg
     3961 ggggaagttg gtgccgcgca tcttcacctt gtagatcagc gtgccgtcct gcagggagga
     4021 gtcctgggtc acggtcacca gaccgccgtc ctcgaagttc atcacgcgct cccacttgaa
     4081 gccctcgggg aaggacagct tcttgtaatc ggggatgtcg gcggggtgct tcacgtacgc
     4141 cttggagccg tacatgaact ggggggacag gatgtcccag gcgaagggca gggggccgcc
     4201 cttggtcacc ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc
     4261 ctcgatctcg aactcgtggc cgttcatgga gccctccatg cgcaccttga agcgcatgaa
     4321 ctctttgatg acggccatgt tgttgtcctc ggaggaggcg gtgccggagc tgccgctgcc
     4381 ggtgctgccg gtgccatgcc ccaggaacag gtggtggcgg ccctcggagc gctcgtactg
     4441 ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc agcttggtgt ccacgtagta
     4501 gtagccgggc agttgcacgg gcttcttggc catgtagatg gtcttgaact ccaccaggta
     4561 gtggccgccg tccttcagct tcagggcctg gtggatctcg cccttcagca cgccgtcgcg
     4621 ggggtacagg cgctcggtgg aggcctccca gcccatggtc ttcttctgca ttacggggcc
     4681 gtcggggggg aagttggtgc cgcgcatctt caccttgtag atcagcgtgc cgtcctgcag
     4741 ggaggagtcc tgggtcacgg tcaccagacc gccgtcctcg aagttcatca cgcgctccca
     4801 cttgaagccc tcggggaagg acagcttctt gtaatcgggg atgtcggcgg ggtgcttcac
     4861 gtacgccttg gagccgtaca tgaactgggg ggacaggatg tcccaggcga agggcagggg
     4921 gccgcccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc
     4981 ctcgccctcg atctcgaact cgtggccgtt catggagccc tccatgcgca ccttgaagcg
     5041 catgaactct ttgatgacct cctcgccctt gctcaccatg atcttttggt cctcatcatt
     5101 cttttcaacc tgtttggttc ttctcataca gcacagcatg gcgcgtgtag gcccggggtt
     5161 ttcttcaaca tcacctgctt gctttagcag agagaagttt gtggcgccgg atcccgggcc
     5221 cgcggtaccg tcgacgtact tgtacagctc gtccatgccg agagtgatcc cggcggcggt
     5281 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga
     5341 ctggtagctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt
     5401 ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa
     5461 gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta
     5521 gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag
     5581 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt
     5641 gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt
     5701 gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg aagcactgca ggccgtagcc
     5761 gaaggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt
     5821 cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg
     5881 gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc
     5941 gcccttgctc accatggtgg cgaccggtga tatctcgccc agatccttga gaagcaggtt
     6001 caggatctcc gtggccctct tcttctggtg gttgctaagc tcctgtagct gactcagctc
     6061 ccgctgggtg gttgtcagtg tcgtcgtttt ctgggccagc tcatcggtca gttgctcgtt
     6121 ggcccgggtc ttgtcctcca cttcctgtga cttctggtcg taattgacag ccagctcctc
     6181 cagggcctgg aggacctcct tcacctcgtc tttagccgcc tcgttttcaa tctggaggcg
     6241 ggtcagctcc tcctggattt tctcgtagtc ccttctggtg gaagctagaa gctcgtcctg
     6301 atccaacatc tgttgcttca gcttttcagc cagctggctc tgctggttaa tttcatcgtc
     6361 cttgtcatcc agctgtcggt acagactggt gatttcctcg tcatacttct ctttctcagc
     6421 agagatgccg tcaaccacag gtgtgatgtt gtctatgatg ggtgtgttgt cgcagggctc
     6481 caggttcttc tggtccttgg cactgatctg ttcatcctca ggcacagctt ccccgttcct
     6541 ccacctgttc agctccacct ccagatgctg gatgacactc ttcagggcct tattcttctc
     6601 tttctctttt tcatatttct tcttccattc ttctgctgtt agttccaagt tcacagagac
     6661 cgtattcttg atggtctttg ctctctgtcc aaacatcagc gtggacttgg tctcagcctc
     6721 attgaagact gaaggagaac agcaaatgac aatggtggtc ctgcagttcc cacccagaga
     6781 gtcctggaga atccgagtca tcttgctgtc ccggtatggc acatgtgttt ttgtcccttc
     6841 tgccaaggca gagatcacat ttccaagagc agacaaagac ttgttgatat ttttagcttc
     6901 atcaagaaca gctccctcgg caccagtttt gctgaccttt tcactcccag ccaaatcaac
     6961 caaatacagc ttcccgctga gttttttttc agtctccaca ttctcttgtt taatgttaat
     7021 caggaagata ctgtgactcc tggagctgtg ttcgttcatg tttgtcacag ccacatgtcg
     7081 gtttgctttg ccctcatcga tcacatccat gacctcctcc gggcttgaca caaacctctc
     7141 ggtgcacccc tttacatagg ggactctgtt tttgtcttca tgaacagcca agttggtctt
     7201 ggacacgtca agcaagtccc ttattttgtc caagtagatc tcaaaatagg aaaccttgat
     7261 atgaaactcc aagttctcat ccatggaata gatgtgatcg aaaatatcgt gtgcgatcct
     7321 gggtatgata cccataagct gaggatcgtg cagcttcccc tccatggtat gagtttttcc
     7381 tgatgaagtc tgcccgtatg caaaaattgt cccattgtaa ccctcgagga catctttgac
     7441 aatctgcttc gcacaggcat tgtagacctg ctcctgggtt gtgttgggcg gcagaactcg
     7501 gtcaaagaca tacggcttcc cttgcccgat caccaccgtc tcctcgcctt tgaatttggg
     7561 gatgaatttg tccccgcgga ggatctcagc ttcgttgagg ggccggaacc ggcacatcac
     7621 tttgatgctg cattcggctg gatccgccat ggcgcgccgc gataacttcg tataaagtat
     7681 cctatacgaa gttatttgcc ttaacccaga aattatcact gttattcttt agaatggtgc
     7741 aaagaataac ttcgtataat gtatgctata cgaagttatg tttaaaccaa tcaacctctg
     7801 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     7861 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7921 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7981 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     8041 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     8101 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     8161 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     8221 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     8281 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     8341 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcgagcttgt
     8401 taacatcgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
     8461 ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata
     8521 cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg
     8581 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     8641 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     8701 ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc
     8761 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
     8821 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
     8881 cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
     8941 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
     9001 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttccgact tgtggtctcg
     9061 ctgccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt cttcacatgc
     9121 agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa tggccatagt
     9181 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggc gctcttccgc
     9241 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     9301 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     9361 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     9421 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     9481 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     9541 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9601 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9661 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9721 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9781 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9841 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9901 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9961 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    10021 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    10081 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt tgcggccgca
    10141 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    10201 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    10261 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    10321 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    10381 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    10441 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    10501 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    10561 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    10621 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10681 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10741 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
    10801 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10861 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10921 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10981 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    11041 tactcttcct ttttcaat
//
