LOCUS       sequence-170965-       17542 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sucrose biosensor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17542)
  AUTHORS   Pini F, East AK, Appia-Ayme C, Tomek J, Karunakaran R, 
            Mendoza-Suarez M, Edwards A, Terpolilli JJ, Roworth J, Downie JA, 
            Poole PS
  TITLE     Bacterial Biosensors for in Vivo Spatiotemporal Mapping of Root 
            Secretion.
  JOURNAL   Plant Physiol. 2017 Jul;174(3):1289-1306. doi: 10.1104/pp.16.01302. 
            Epub 2017 May 11.
  PUBMED    28495892
REFERENCE   2  (bases 1 to 17542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.16.01302"; journalName: "Plant Physiol"; date: 
            "2017-07"; volume: "174"; issue: "3"; pages: "1289-1306"
COMMENT     Sequence Label: pLMB687
FEATURES             Location/Qualifiers
     source          1..17542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(265..915)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             1021..2220
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(join(2747..3538,3539..3607))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3370..3389
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3608..3712)
                     /gene="bla"
                     /label=AmpR promoter
     oriT            3889..3997
                     /note="incP origin of transfer"
     primer_bind     4042..4060
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    4185..4206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4221..4226,4227..4244,4245..4251)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4259..4275
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4264..4286
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4283..4299
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4283..4299
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             8941..10383
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             10396..11319
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             11368..12450
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             12465..13448
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             13627..14739
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     CDS             complement(14791..15939)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      16606..17317
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 ccgccctgga cctcgcccgc gagcgccagg cgcacgaggc cggcgcgcgg acccgcgcca
       61 cggcccacga gcggacgccg cagcaggagc gccagaaggc cgccagagag gccgagcgcg
      121 gccgtgaggc ttggacgcta gggcagggca tgaaaaagcc cgtagcgggc tgctacgggc
      181 gtctgacgcg gtggaaaggg ggaggggatg ttgtctacat ggctctgctg tagtgagtgg
      241 gttgcgctcc ggcagcggtc ctgatcaatc gtcacccttt ctcggtcctt caacgttcct
      301 gacaacgagc ctccttttcg ccaatccatc gacaatcacc gcgagtccct gctcgaacgc
      361 tgcgtccgga ccggcttcgt cgaaggcgtc tatcgcggcc cgcaacagcg gcgagagcgg
      421 agcctgttca acggtgccgc cgcgctcgcc ggcatcgctg tcgccggcct gctcctcaag
      481 cacggcccca acagtgaagt agctgattgt catcagcgca ttgacggcgt ccccggccga
      541 aaaacccgcc tcgcagagga agcgaagctg cgcgtcggcc gtttccatct gcggtgcgcc
      601 cggtcgcgtg ccggcatgga tgcgcgcgcc atcgcggtag gcgagcagcg cctgcctgaa
      661 gctgcgggca ttcccgatca gaaatgagcg ccagtcgtcg tcggctctcg gcaccgaatg
      721 cgtatgattc tccgccagca tggcttcggc cagtgcgtcg agcagcgccc gcttgttcct
      781 gaagtgccag taaagcgccg gctgctgaac ccccaaccgt tccgccagtt tgcgtgtcgt
      841 cagaccgtct acgccgacct cgttcaacag gtccagggcg gcacggatca ctgtattcgg
      901 ctgcaacttt gtcatgcttg acactttatc actgataaac ataatatgtc caccaactta
      961 tcagtgataa agaatccgcg cgttcaatcg gaccagcgga ggctggtccg gaggccagac
     1021 gtgaaaccca acatacccct gatcgtaatt ctgagcactg tcgcgctcga cgctgtcggc
     1081 atcggcctga ttatgccggt gctgccgggc ctcctgcgcg atctggttca ctcgaacgac
     1141 gtcaccgccc actatggcat tctgctggcg ctgtatgcgt tggtgcaatt tgcctgcgca
     1201 cctgtgctgg gcgcgctgtc ggatcgtttc gggcggcggc caatcttgct cgtctcgctg
     1261 gccggcgcca ctgtcgacta cgccatcatg gcgacagcgc ctttcctttg ggttctctat
     1321 atcgggcgga tcgtggccgg catcaccggg gcgactgggg cggtagccgg cgcttatatt
     1381 gccgatatca ctgatggcga tgagcgcgcg cggcacttcg gcttcatgag cgcctgtttc
     1441 gggttcggga tggtcgcggg acctgtgctc ggtgggctga tgggcggttt ctccccccac
     1501 gctccgttct tcgccgcggc agccttgaac ggcctcaatt tcctgacggg ctgtttcctt
     1561 ttgccggagt cgcacaaagg cgaacgccgg ccgttacgcc gggaggctct caacccgctc
     1621 gcttcgttcc ggtgggcccg gggcatgacc gtcgtcgccg ccctgatggc ggtcttcttc
     1681 atcatgcaac ttgtcggaca ggtgccggcc gcgctttggg tcattttcgg cgaggatcgc
     1741 tttcactggg acgcgaccac gatcggcatt tcgcttgccg catttggcat tctgcattca
     1801 ctcgcccagg caatgatcac cggccctgta gccgcccggc tcggcgaaag gcgggcactc
     1861 atgctcggaa tgattgccga cggcacaggc tacatcctgc ttgccttcgc gacacgggga
     1921 tggatggcgt tcccgatcat ggtcctgctt gcttcgggtg gcatcggaat gccggcgctg
     1981 caagcaatgt tgtccaggca ggtggatgag gaacgtcagg ggcagctgca aggctcactg
     2041 gcggcgctca ccagcctgac ctcgatcgtc ggacccctcc tcttcacggc gatctatgcg
     2101 gcttctataa caacgtggaa cgggtgggca tggattgcag gcgctgccct ctacttgctc
     2161 tgcctgccgg cgctgcgtcg cgggctttgg agcggcgcag ggcaacgagc cgatcgctga
     2221 tcgtggaaac gataggccgg aattgacgga tctatctcat ctgcgcaagg cagaacgtga
     2281 agacggccgc cctggacctc gcccgcgagc gccaggcgca cgaggccggc gcgcggaccc
     2341 gcgccacggc ccacgagcgg acgccgcagc aggagcgcca gaaggccgcc agagaggccg
     2401 agcgcggccg tgaggcttgg acgctagggc agggcatgaa aaagcccgta gcgggctgct
     2461 acgggcgtct gacgcggtgg aaagggggag gggatgttgt ctacatggct ctgctgtagt
     2521 gagtgggttg cgctccggca gcggtcctga tcccccgcag aaaaaaagga tctcaagaag
     2581 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2641 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     2701 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     2761 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     2821 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     2881 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2941 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3001 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     3061 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     3121 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     3181 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     3241 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     3301 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     3361 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3421 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3481 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3541 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3601 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3661 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3721 cccgaaaagt gccacctggg tcgatcgacg gatcttttcc gctgcataac cctgcttcgg
     3781 ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata caggattttg
     3841 ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg gcaggatagg
     3901 tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt cgcacctggc
     3961 ggtgctcaac gggaatcctg ctctgcgagg ctggccgata agctctaaga aaccattatt
     4021 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaatta
     4081 attccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     4141 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     4201 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     4261 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctgatc
     4321 ccccgatgtg tagcagtggc ggaccatata ggcagatcag aaggcgcggt tctcctacat
     4381 gttcgccggt gaacgcgttg aggaagccgg gcagtgcctc ggcaaaatcc ttgcgtgtag
     4441 acaagacatc tgcgtagcag ttgtcctcaa caacgatgtc gaaatccaaa tcggagtgct
     4501 catcgagtcc tccgtgaacg taagagccgc cgatcagaag agcgcggaag cgaacatcgg
     4561 aagcgaccgc atcgcggatg cggttcaaga aagttgcatg agcttgtgga agtgtgctga
     4621 gcataaatga ttctcctagc tgttctttgg gtaagtacgc catcaggacg ttgtgagtgg
     4681 cgcgattttt agcggctgaa atcagccctt gagcctgtcg gcaagtcgcg tcatgaggtc
     4741 catgcgctca tgcaggatcg ccacgaccaa cgcgggttcg cccgcacgcg gcaggcaaaa
     4801 aacgtagtgg tgttcgcagc gggccatccg cagcgcggga aagagttcgc tcatgtcctt
     4861 aaacgggcct tcgccggcgg caagcctggc tatgccctgt tccagcttag cgatatagcg
     4921 gcgcacctgc gccgcgcccc actcccggcg cgtgtagcgg atgatgccgc gtagatcggc
     4981 ttcggcctca gccgtgagga tgtaggccgt caagcgcgat ccccgctgag ttcttcatca
     5041 agaatttcgc cgacgctctt ggtggacacc ttgccggcaa gcccatcgtt gatgcggttc
     5101 cccagcatgg ttttcagttc ctgccatgcc tgatcggcat cagcgtcacc ggggaacaga
     5161 cgttcgaggg cgtattgctt aatggtcttg ccctgcaagg cggccagggc tttcaggctc
     5221 tggtgctgct ggtccgtcat gtcgattgtc aggcggctca ttggataacc tccataaaat
     5281 acacgtaacc acattagcac atatgtgggc gtgaggctac agcgcgaggc gcattaaggt
     5341 cgggaaaatg cgctaggcgc atttaaattg cgtattgctg taatgcgcca tgccggctag
     5401 actaggccca aatgggtata cccaatttga ccaaggggga cgcgatgagg gcggccaagc
     5461 actaccgaca acttctatcc atcgacttca acatcgaggc gctggccttc gtgcctggac
     5521 ccgacggcac acgcggccgg cgcatccacg tcctggggcg cgaggtccgc gaccggcccg
     5581 gcctggtcga gtacctttcg ccggcgttcg gctcgcgggt ggcgctggac ggctactgca
     5641 aggccaattt cgatgcagtg ctgcacctgg cgtaccccga tcatcagcaa tggggccacg
     5701 catgaagcgc cgaagctacg ccatgctgcg cgccgctgcc gcgctggccg tcctggtcgt
     5761 tgcctcgccg gcatgggccg agctgcgcgg cgaggtcgtg cgcatcatcg acggcgacac
     5821 catcgacgtg ctggtagaca agcagccggt gcgcgtgcgc ctggtggaca ttgacgcgcc
     5881 ggaaaagcgg caagccttcg gcgaacgtgc gcgccaggcg ctggccggca tggtgttccg
     5941 ccggcacgtc ctggtcgacg agaaggacac cgaccgttac ggccgcacgc tgggcaccgt
     6001 gtgggtcaac atggagctgg ccagccggcc gccgcagccg cgcaacgtca acgccgcgat
     6061 ggttcaccag ggcatggcgt gggcctatcg cttccacggc cgcgcggccg accctgaaat
     6121 gctgcggctc gaacaggagg cgcgaggcaa gcgcgtcggc ctctggtccg atccgcacgc
     6181 cgtcgagccg tggaaatggc gacgcgagag caacaaccgg agggacgaag gttgaaggtc
     6241 gcccgcatct acctgcgcgc cagtacggac gagcagaatc ttgaacgcca ggagagcctt
     6301 gtagcggcca cgcgggccgc cgggtactac gtcgccggca tctaccgcga gaaggcgtcc
     6361 ggcgcacgcg ccgaccggcc cgagctgctg cgcatgatcg cggacctgca acctggtgaa
     6421 gtcgtcgttg cggagaagat cgaccgcatc agccgcttgc cgttggccga ggccgagcgc
     6481 ctggttgcgt cgatccgggc caaaggggcc aagctggccg tgcctggcgt ggtggacctg
     6541 tcggagctgg ccgccgaggc gaacggagtg gcgaaaatcg ttctggaatc cgtccaggac
     6601 atgcttttga agctcgcctt gcagatggcc cgcgacgact acgaggatcg gcgcgagcgt
     6661 caacgtcagg gtgtccagtt ggcgaaggcc gccggccgct acaccggccg caaacgtgac
     6721 gccggcatgc acgaccgcat catcacgctt cgctccggcg gatcgagcat tgccaagacg
     6781 gccaagctgg tcggatgcag cccgagccag gtcaaacgag tgtgggcggc ctggaacgcg
     6841 cagcagcaaa aataaagccg ggcagtgccc ggcttttctc accttttcgc gtcccgcagg
     6901 gccgctgcga gcgccctacc tagatcctcg ctttccccct cggtgtagtc cggccagggc
     6961 acgaagggcg cggatgcgaa cctgttgagc aggtacgcct tcgggcagcg gtagaccacc
     7021 ggcgagttcg ccttttcatc ccaccgggcc aggatcacgt ccgcatcgca gtgcatgtcc
     7081 ttcacctggt cgcggaagaa gccgaaggcc accatgccgc tatgttcgcc gaggaacgcc
     7141 agttgcttcg cgctggcgat cgcgccgacg ccgccggcca aaaccgacgc catcacccag
     7201 ccgacgaacc agaagctggc atgcttgcgg ttgaccaccg cacgcgcagc cgcgaccagg
     7261 acaacggcca agctgccgac cagggccatg acgaccgtga tccggccgtt gtggaaagcg
     7321 atgggcttgc cagcgtccgc ttgcacggcg tcgtaaatgc tggacccgat gggcgcgcac
     7381 atcagcacga caggcagcag caccaggaac atcgtccgcg tccattgcgc gagtgccttg
     7441 cggcgttcgc cggcggcaag cgcctccatc atcggcgtga agcccaacag ggccaccgca
     7501 gccgccaagc cggcaacgat gccgcaggcg attacataca tacatcctcc ctaatgcgcc
     7561 ttgcgcacgg ttgtagtcag agtccgcggt gggggtaccg taaaagccgc ttgacaaaaa
     7621 gcgttctccg cgactattgg gtaatcgatc acccaaggag gatggtgcgg atcgtggcag
     7681 tatctctttc tgacatcgcg gaacgtgcgg gcgtctcggt aaagacggtt tccggtgcat
     7741 tgcacggcgg ctccgcccgc atgtcggacg ataagcgcca gaaggtcaag gccattgcgg
     7801 aagagctcgg ctacgtgacc aaccttgcgg cgcgtggcgt gcgacagggc tggctgccgc
     7861 tggtcggcgt tgtctccgac ggcctcatca cttcgccctt cgccaccgag atcgtacgtg
     7921 gcctcgacgg ggcggcccgc agcgccggca tggcggtctt tgcggtcaac caccgcagcg
     7981 gccaatctat cggctcggtg ctcgacgagg tgcagcagtt ccggccgcgc gccgttgctt
     8041 atgccgccat gtaccacaag gatgtcgcgc tgccggacag gttggcgggc gccgtcggcg
     8101 tcatgatcaa ctgccgagag gctaccggcc gcgtcaccgc gctggtgccg gacgaagagg
     8161 gcggcgcgcg ggagatcgtg cgttatctcc tcgtcgccgg ccgccggcgc attgccttca
     8221 tcaacctgcc aggcattctc gctggcacgc tacgcgagat ggggtttcgt gcggcgcttg
     8281 gggaggcggg catcgaccac gtcggggtgt ttccggcggt gcgccgcagc gtctacagcg
     8341 accgcgcgcc aagccttgtt gcctctcatg tggaggcgct tctcaaatcc ggtcaacggc
     8401 ctgacgccat cttgtgcggc aatgaccgcg tggcgatgga agtctatgcg gcgcttgccc
     8461 gcgccggcct tcgcatcccg gatgatatcg cggtggcaag cttcgacaac caggtcgaga
     8521 ttgcctcgcg gctggacccg ccattgacga cgatggccct gccgcaccgc gccatgggcc
     8581 gccttgccat ggagatcctc ctgaccgagc agcccgaacc gccgcatctg cataaactgc
     8641 cgttccatct ggtcgaacgg gcatccgtct gaacatcgaa tagtgccaaa caggaggaga
     8701 atacgaaatg aggattttaa tgggtaatcg tttactttcc gcactcttag cgtccgctgc
     8761 cctggtcgcg accgcgggtt tcgccgaggc gaagaccgtc atccacgtca tgcaccaggg
     8821 cgatcccggc tgggtgaagg cctatggcga cgttgccacc cgctttgagg ccgccaatcc
     8881 cgatgtcgac atcgagatga tctggatccg tttaaacgaa ttcaggcttg gaggatacgt
     8941 atgactaaaa aaatttcatt cattattaac ggccaggttg aaatctttcc cgaaggtgat
     9001 gatttagtgc aatccattaa ttttggtgat aatagtgttt acctgccaat attgaatgac
     9061 tctcatgtaa aaaacattat tgattgtaat ggaaataacg aattacggtt gcataacatt
     9121 gtcaattttc tctatacggt agggcaaaga tggaaaaatg aagaatactc aagacgcagg
     9181 acatacattc gtgacttaaa aaaatatatg ggatattcag aagaaatggc taagctagag
     9241 gccaattgga tatctatgat tttatgttct aaaggcggcc tttatgatgt tgtagaaaat
     9301 gaacttggtt ctcgccatat catggatgaa tggctacctc aggatgaaag ttatgttcgg
     9361 gcttttccga aaggtaaatc tgtacatctg ttggcaggta atgttccatt atctgggatc
     9421 atgtctatat tacgcgcaat tttaactaag aatcagtgta ttataaaaac atcgtcaacc
     9481 gatcctttta ccgctaatgc attagcgtta agttttattg atgtagaccc taatcatccg
     9541 ataacgcgct ctttatctgt tatatattgg ccccaccaag gtgatacatc actcgcaaaa
     9601 gaaattatgc aacatgcgga tgttattgtc gcttggggag ggccagatgc gattaattgg
     9661 gcggtagagc atgcgccatc ttatgctgat gtgattaaat ttggttctaa aaagagtctt
     9721 tgcattatcg ataatcctgt tgatttgacg tccgcagcga caggtgcggc tcatgatgtt
     9781 tgtttttacg atcagcgagc ttgtttttct gcccaaaaca tatattacat gggaaatcat
     9841 tatgaggaat ttaagttagc gttgatagaa aaacttaatc tatatgcgca tatattaccg
     9901 aatgccaaaa aagattttga tgaaaaggcg gcctattctt tagttcaaaa agaaagcttg
     9961 tttgctggat taaaagtaga ggtggatatt catcaacgtt ggatgattat tgagtcaaat
    10021 gcaggtgtgg aatttaatca accacttggc agatgtgtgt accttcatca cgtcgataat
    10081 attgagcaaa tattgcctta tgttcaaaaa aataagacgc aaaccatatc tatttttcct
    10141 tgggagtcat catttaaata tcgagatgcg ttagcattaa aaggtgcgga aaggattgta
    10201 gaagcaggaa tgaataacat atttcgagtt ggtggatctc atgacggaat gagaccgttg
    10261 caacgattag tgacatatat ttctcatgaa aggccatcta actatacggc taaggatgtt
    10321 gcggttgaaa tagaacagac tcgattcctg gaagaagata agttccttgt atttgtccca
    10381 taataggtaa aaagtatgga aaatgaatca aaatataaaa ccatcgacca cgttatttgt
    10441 gttgaaggaa ataaaaaaat tcatgtttgg gaaacgctgc cagaagaaaa cagcccaaag
    10501 agaaagaatg ccattattat tgcgtctggt tttgcccgca ggatggatca ttttgctggt
    10561 ctggcggaat atttatcgcg gaatggattt catgtgatcc gctatgattc gcttcaccac
    10621 gttggattga gttcagggac aattgatgaa tttacaatgt ctataggaaa gcagagcttg
    10681 ttagcagtgg ttgattggtt aactacacga aaaataaata acttcggtat gttggcttca
    10741 agcttatctg cgcggatagc ttatgcaagc ctatctgaaa tcaatgcttc gtttttaatc
    10801 accgcagtcg gtgttgttaa cttaagatat tctcttgaaa gagctttagg gtttgattat
    10861 ctcagtctac ccattaatga attgccgaat aatctagatt ttgaaggcca taaattgggt
    10921 gctgaagtct ttgcgagaga ttgtcttgat tttggttggg aagatttagc ttctacaatt
    10981 aataacatga tgtatcttga tataccgttt attgctttta ctgcaaataa cgataattgg
    11041 gtcaagcaag atgaagttat cacattgtta tcaaatattc gtagtaatcg atgcaagata
    11101 tattctttgt taggaagttc gcatgacttg agtgaaaatt tagtggtcct gcgcaatttt
    11161 tatcaatcgg ttacgaaagc cgctatcgcg atggataatg atcatctgga tattgatgtt
    11221 gatattactg aaccgtcatt tgaacattta actattgcga cagtcaatga acgccgaatg
    11281 agaattgaga ttgaaaatca agcaatttct ctgtcttaaa atctattgag atattctatc
    11341 actcaaatag caatataagg actctctatg aaatttggaa actttttgct tacataccaa
    11401 cctccccaat tttctcaaac agaggtaatg aaacgtttgg ttaaattagg tcgcatctct
    11461 gaggagtgtg gttttgatac cgtatggtta ctggagcatc atttcacgga gtttggtttg
    11521 cttggtaacc cttatgtcgc tgctgcatat ttacttggcg cgactaaaaa attgaatgta
    11581 ggaactgccg ctattgttct tcccacagcc catccagtac gccaacttga agatgtgaat
    11641 ttattggatc aaatgtcaaa aggacgattt cggtttggta tttgccgagg gctttacaac
    11701 aaggactttc gcgtattcgg cacagatatg aataacagtc gcgccttagc ggaatgctgg
    11761 tacgggctga taaagaatgg catgacagag ggatatatgg aagctgataa tgaacatatc
    11821 aagttccata aggtaaaagt aaaccccgcg gcgtatagca gaggtggcgc accggtttat
    11881 gtggtggctg aatcagcttc gacgactgag tgggctgctc aatttggcct accgatgata
    11941 ttaagttgga ttataaatac taacgaaaag aaagcacaac ttgagcttta taatgaagtg
    12001 gctcaagaat atgggcacga tattcataat atcgaccatt gcttatcata tataacatct
    12061 gtagatcatg actcaattaa agcgaaagag atttgccgga aatttctggg gcattggtat
    12121 gattcttatg tgaatgctac gactattttt gatgattcag accaaacaag aggttatgat
    12181 ttcaataaag ggcagtggcg tgactttgta ttaaaaggac ataaagatac taatcgccgt
    12241 attgattaca gttacgaaat caatcccgtg ggaacgccgc aggaatgtat tgacataatt
    12301 caaaaagaca ttgatgctac aggaatatca aatatttgtt gtggatttga agctaatgga
    12361 acagtagacg aaattattgc ttccatgaag ctcttccagt ctgatgtcat gccatttctt
    12421 aaagaaaaac aacgttcgct attatattag ctaaggagaa agaaatgaaa tttggattgt
    12481 tcttccttaa cttcatcaat tcaacaactg ttcaagaaca aagtatagtt cgcatgcagg
    12541 aaataacgga gtatgttgat aagttgaatt ttgaacagat tttagtgtat gaaaatcatt
    12601 tttcagataa tggtgttgtc ggcgctcctc tgactgtttc tggttttctg ctcggtttaa
    12661 cagagaaaat taaaattggt tcattaaatc acatcattac aactcatcat cctgtccgca
    12721 tagcggagga agcttgctta ttggatcagt taagtgaagg gagatttatt ttagggttta
    12781 gtgattgcga aaaaaaagat gaaatgcatt tttttaatcg cccggttgaa tatcaacagc
    12841 aactatttga agagtgttat gaaatcatta acgatgcttt aacaacaggc tattgtaatc
    12901 cagataacga tttttatagc ttccctaaaa tatctgtaaa tccccatgct tatacgccag
    12961 gcggacctcg gaaatatgta acagcaacca gtcatcatat tgttgagtgg gcggccaaaa
    13021 aaggtattcc tctcatcttt aagtgggatg attctaatga tgttagatat gaatatgctg
    13081 aaagatataa agccgttgcg gataaatatg acgttgacct atcagagata gaccatcagt
    13141 taatgatatt agttaactat aacgaagata gtaataaagc taaacaagag acgcgtgcat
    13201 ttattagtga ttatgttctt gaaatgcacc ctaatgaaaa tttcgaaaat aaacttgaag
    13261 aaataattgc agaaaacgct gtcggaaatt atacggagtg tataactgcg gctaagttgg
    13321 caattgaaaa gtgtggtgcg aaaagtgtat tgctgtcctt tgaaccaatg aatgatttga
    13381 tgagccaaaa aaatgtaatc aatattgttg atgataatat taagaagtac cacatggaat
    13441 atacctaata gatttcgagt tgcagcgagg cggcaagtga acgaatcccc aggagcatag
    13501 ataactatgt gactggggtg agtgaaagca gccaacaaag cagcagcttg aaagatgaag
    13561 ggtataaaag agtatgacag cagtgctgcc atactttcta atattatctt gaggagtaaa
    13621 acaggtatga cttcatatgt tgataaacaa gaaattacag caagctcaga aattgatgat
    13681 ttgatttttt cgagcgatcc attagtgtgg tcttacgacg agcaggaaaa aatcagaaag
    13741 aaacttgtgc ttgatgcatt tcgtaatcat tataaacatt gtcgagaata tcgtcactac
    13801 tgtcaggcac acaaagtaga tgacaatatt acggaaattg atgacatacc tgtattccca
    13861 acatcggttt ttaagtttac tcgcttatta acttctcagg aaaacgagat tgaaagttgg
    13921 tttaccagta gcggcacgaa tggtttaaaa agtcaggtgg cgcgtgacag attaagtatt
    13981 gagagactct taggctctgt gagttatggc atgaaatatg ttggtagttg gtttgatcat
    14041 caaatagaat tagtcaattt gggaccagat agatttaatg ctcataatat ttggtttaaa
    14101 tatgttatga gtttggtgga attgttatat cctacgacat ttaccgtaac agaagaacga
    14161 atagattttg ttaaaacatt gaatagtctt gaacgaataa aaaatcaagg gaaagatctt
    14221 tgtcttattg gttcgccata ctttatttat ttactctgcc attatatgaa agataaaaaa
    14281 atctcatttt ctggagataa aagcctttat atcataaccg gaggcggctg gaaaagttac
    14341 gaaaaagaat ctctgaaacg tgatgatttc aatcatcttt tatttgatac tttcaatctc
    14401 agtgatatta gtcagatccg agatatattt aatcaagttg aactcaacac ttgtttcttt
    14461 gaggatgaaa tgcagcgtaa acatgttccg ccgtgggtat atgcgcgagc gcttgatcct
    14521 gaaacgttga aacctgtacc tgatggaacg ccggggttga tgagttatat ggatgcgtca
    14581 gcaaccagtt atccagcatt tattgttacc gatgatgtcg ggataattag cagagaatat
    14641 ggtaagtatc ccggcgtgct cgttgaaatt ttacgtcgcg tcaatacgag gacgcagaaa
    14701 gggtgtgctt taagcttaac cgaagcgttt gatagttgat gacctgcagg tggctgctga
    14761 acccccagcc ggaactgacc ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt
    14821 gacccaggcg tgttccacca ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg
    14881 ctcgcgccac ttcttcacgc gggtggaatc cgatccgcac atgaggcgga aggtttccag
    14941 cttgagcggg tacggctccc ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg
    15001 cgacagcttg cggtacttct cccatatgaa tttcgtgtag tggtcgccag caaacagcac
    15061 gacgatttcc tcgtcgatca ggacctggca acgggacgtt ttcttgccac ggtccaggac
    15121 gcggaagcgg tgcagcagcg acaccgattc caggtgccca acgcggtcgg acgtgaagcc
    15181 catcgccgtc gcctgtaggc gcgacaggca ttcctcggcc ttcgtgtaat accggccatt
    15241 gatcgaccag cccaggtcct ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat
    15301 aggggtgcgc ttcgcgtact ccaacacctg ctgccacacc agttcgtcat cgtcggcccg
    15361 cagctcgacg ccggtgtagg tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt
    15421 ttgcagcgcc tcgcgcggga ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt
    15481 gtcgtttggc atcgctcgca tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg
    15541 catttccttg atctgctgct tcgtgtgttt cagcaacgcg gcctgcttgg cctcgctgac
    15601 ctgttttgcc aggtcctcgc cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt
    15661 gtcgatggtc atcgacttcg ccaaacctgc cgcctcctgt tcgagacgac gcgaacgctc
    15721 cacggcggcc gatggcgcgg gcagggcagg gggagccagt tgcacgctgt cgcgctcgat
    15781 cttggccgta gcttgctgga ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg
    15841 catgacggtg cggcttgcga tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag
    15901 ttcttgcctg tatgccttcc ggtcaaacgt ccgattcatt caccctcctt gcgggattgc
    15961 cccggaattc cccggatcga tccgtcgatc ttgatcccct gcgccatcag atccttggcg
    16021 gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag
    16081 ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat cgccatgtaa
    16141 gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc cagatagccc
    16201 agtagctgac attcatccgg ggtcagcacc gtttctgcgg actggctttc tacgtgttcc
    16261 gcttccttta gcagcccttg cgccctgagt gcttgcggca gcgtgaagct gacggatcga
    16321 tccggggaat taattcaccc ccgaacacga gcacggcacc cgcgaccact atgccaagaa
    16381 tgcccaaggt aaaaattgcc ggccccgcca tgaagtccgt gaatgccccg acggccgaag
    16441 tgaagggcag gccgccaccc aggccgccgc cctcactgcc cggcacctgg tcgctgaatg
    16501 tcgatgccag cacctgcggc acgtcaatgc ttccgggcgt cgcgctcggg ctgatcgccc
    16561 atcccgttac tgccccgatc ccggcaatgg caaggactgc cagcgctgcc atttttgggg
    16621 tgaggccgtt cgcggccgag gggcgcagcc cctgggggga tgggaggccc gcgttagcgg
    16681 gccgggaggg ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg
    16741 gcgcagccct ggttaaaaac aaggtttata aatattggtt taaaagcagg ttaaaagaca
    16801 ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg
    16861 gacagcccct caaatgtcaa taggtgcgcc cctcatctgt cagcactctg cccctcaagt
    16921 gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg
    16981 gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt
    17041 tcgccgattt gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc ctgcccctca
    17101 tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg
    17161 tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc ggcggccgcg gtgtctcgca
    17221 cacggcttcg acggcgtttc tggcgcgttt gcagggccat agacggccgc cagcccagcg
    17281 gcgagggcaa ccagcccggt gagcgtcgga aaggcgctgg aagccccgta gcgacgcgga
    17341 gaggggcgag acaagccaag ggcgcaggct cgatgcgcag cacgacatag ccggttctcg
    17401 caaggacgag aatttccctg cggtgcccct caagtgtcaa tgaaagtttc caacgcgagc
    17461 cattcgcgag agccttgagt ccacgctaga tccgtcaatt ccgggatcta tctcatctgc
    17521 gcaaggcaga acgtgaagac gg
//
