LOCUS       sequence-170966-       17538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sucrose biosensor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17538)
  AUTHORS   Pini F, East AK, Appia-Ayme C, Tomek J, Karunakaran R, 
            Mendoza-Suarez M, Edwards A, Terpolilli JJ, Roworth J, Downie JA, 
            Poole PS
  TITLE     Bacterial Biosensors for in Vivo Spatiotemporal Mapping of Root 
            Secretion.
  JOURNAL   Plant Physiol. 2017 Jul;174(3):1289-1306. doi: 10.1104/pp.16.01302. 
            Epub 2017 May 11.
  PUBMED    28495892
REFERENCE   2  (bases 1 to 17538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.16.01302"; journalName: "Plant Physiol"; date: 
            "2017-07"; volume: "174"; issue: "3"; pages: "1289-1306"
COMMENT     Sequence Label: pLMB687
FEATURES             Location/Qualifiers
     source          1..17538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             33..1475
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             1488..2411
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             2460..3542
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             3557..4540
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             4719..5831
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     CDS             complement(5883..7031)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      7698..8409
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(8899..9549)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             9655..10854
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(join(11377..12168,12169..12237))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12000..12019
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12238..12342)
                     /gene="bla"
                     /label=AmpR promoter
     oriT            12519..12627
                     /note="incP origin of transfer"
     primer_bind     12672..12690
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    12815..12836
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12851..12856,12857..12874,12875..12881)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12889..12905
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12894..12916
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12913..12929
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12913..12929
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cgtttaaacg aattcaggct tggaggatac gtatgactaa aaaaatttca ttcattatta
       61 acggccaggt tgaaatcttt cccgaaggtg atgatttagt gcaatccatt aattttggtg
      121 ataatagtgt ttacctgcca atattgaatg actctcatgt aaaaaacatt attgattgta
      181 atggaaataa cgaattacgg ttgcataaca ttgtcaattt tctctatacg gtagggcaaa
      241 gatggaaaaa tgaagaatac tcaagacgca ggacatacat tcgtgactta aaaaaatata
      301 tgggatattc agaagaaatg gctaagctag aggccaattg gatatctatg attttatgtt
      361 ctaaaggcgg cctttatgat gttgtagaaa atgaacttgg ttctcgccat atcatggatg
      421 aatggctacc tcaggatgaa agttatgttc gggcttttcc gaaaggtaaa tctgtacatc
      481 tgttggcagg taatgttcca ttatctggga tcatgtctat attacgcgca attttaacta
      541 agaatcagtg tattataaaa acatcgtcaa ccgatccttt taccgctaat gcattagcgt
      601 taagttttat tgatgtagac cctaatcatc cgataacgcg ctctttatct gttatatatt
      661 ggccccacca aggtgataca tcactcgcaa aagaaattat gcaacatgcg gatgttattg
      721 tcgcttgggg agggccagat gcgattaatt gggcggtaga gcatgcgcca tcttatgctg
      781 atgtgattaa atttggttct aaaaagagtc tttgcattat cgataatcct gttgatttga
      841 cgtccgcagc gacaggtgcg gctcatgatg tttgttttta cgatcagcga gcttgttttt
      901 ctgcccaaaa catatattac atgggaaatc attatgagga atttaagtta gcgttgatag
      961 aaaaacttaa tctatatgcg catatattac cgaatgccaa aaaagatttt gatgaaaagg
     1021 cggcctattc tttagttcaa aaagaaagct tgtttgctgg attaaaagta gaggtggata
     1081 ttcatcaacg ttggatgatt attgagtcaa atgcaggtgt ggaatttaat caaccacttg
     1141 gcagatgtgt gtaccttcat cacgtcgata atattgagca aatattgcct tatgttcaaa
     1201 aaaataagac gcaaaccata tctatttttc cttgggagtc atcatttaaa tatcgagatg
     1261 cgttagcatt aaaaggtgcg gaaaggattg tagaagcagg aatgaataac atatttcgag
     1321 ttggtggatc tcatgacgga atgagaccgt tgcaacgatt agtgacatat atttctcatg
     1381 aaaggccatc taactatacg gctaaggatg ttgcggttga aatagaacag actcgattcc
     1441 tggaagaaga taagttcctt gtatttgtcc cataataggt aaaaagtatg gaaaatgaat
     1501 caaaatataa aaccatcgac cacgttattt gtgttgaagg aaataaaaaa attcatgttt
     1561 gggaaacgct gccagaagaa aacagcccaa agagaaagaa tgccattatt attgcgtctg
     1621 gttttgcccg caggatggat cattttgctg gtctggcgga atatttatcg cggaatggat
     1681 ttcatgtgat ccgctatgat tcgcttcacc acgttggatt gagttcaggg acaattgatg
     1741 aatttacaat gtctatagga aagcagagct tgttagcagt ggttgattgg ttaactacac
     1801 gaaaaataaa taacttcggt atgttggctt caagcttatc tgcgcggata gcttatgcaa
     1861 gcctatctga aatcaatgct tcgtttttaa tcaccgcagt cggtgttgtt aacttaagat
     1921 attctcttga aagagcttta gggtttgatt atctcagtct acccattaat gaattgccga
     1981 ataatctaga ttttgaaggc cataaattgg gtgctgaagt ctttgcgaga gattgtcttg
     2041 attttggttg ggaagattta gcttctacaa ttaataacat gatgtatctt gatataccgt
     2101 ttattgcttt tactgcaaat aacgataatt gggtcaagca agatgaagtt atcacattgt
     2161 tatcaaatat tcgtagtaat cgatgcaaga tatattcttt gttaggaagt tcgcatgact
     2221 tgagtgaaaa tttagtggtc ctgcgcaatt tttatcaatc ggttacgaaa gccgctatcg
     2281 cgatggataa tgatcatctg gatattgatg ttgatattac tgaaccgtca tttgaacatt
     2341 taactattgc gacagtcaat gaacgccgaa tgagaattga gattgaaaat caagcaattt
     2401 ctctgtctta aaatctattg agatattcta tcactcaaat agcaatataa ggactctcta
     2461 tgaaatttgg aaactttttg cttacatacc aacctcccca attttctcaa acagaggtaa
     2521 tgaaacgttt ggttaaatta ggtcgcatct ctgaggagtg tggttttgat accgtatggt
     2581 tactggagca tcatttcacg gagtttggtt tgcttggtaa cccttatgtc gctgctgcat
     2641 atttacttgg cgcgactaaa aaattgaatg taggaactgc cgctattgtt cttcccacag
     2701 cccatccagt acgccaactt gaagatgtga atttattgga tcaaatgtca aaaggacgat
     2761 ttcggtttgg tatttgccga gggctttaca acaaggactt tcgcgtattc ggcacagata
     2821 tgaataacag tcgcgcctta gcggaatgct ggtacgggct gataaagaat ggcatgacag
     2881 agggatatat ggaagctgat aatgaacata tcaagttcca taaggtaaaa gtaaaccccg
     2941 cggcgtatag cagaggtggc gcaccggttt atgtggtggc tgaatcagct tcgacgactg
     3001 agtgggctgc tcaatttggc ctaccgatga tattaagttg gattataaat actaacgaaa
     3061 agaaagcaca acttgagctt tataatgaag tggctcaaga atatgggcac gatattcata
     3121 atatcgacca ttgcttatca tatataacat ctgtagatca tgactcaatt aaagcgaaag
     3181 agatttgccg gaaatttctg gggcattggt atgattctta tgtgaatgct acgactattt
     3241 ttgatgattc agaccaaaca agaggttatg atttcaataa agggcagtgg cgtgactttg
     3301 tattaaaagg acataaagat actaatcgcc gtattgatta cagttacgaa atcaatcccg
     3361 tgggaacgcc gcaggaatgt attgacataa ttcaaaaaga cattgatgct acaggaatat
     3421 caaatatttg ttgtggattt gaagctaatg gaacagtaga cgaaattatt gcttccatga
     3481 agctcttcca gtctgatgtc atgccatttc ttaaagaaaa acaacgttcg ctattatatt
     3541 agctaaggag aaagaaatga aatttggatt gttcttcctt aacttcatca attcaacaac
     3601 tgttcaagaa caaagtatag ttcgcatgca ggaaataacg gagtatgttg ataagttgaa
     3661 ttttgaacag attttagtgt atgaaaatca tttttcagat aatggtgttg tcggcgctcc
     3721 tctgactgtt tctggttttc tgctcggttt aacagagaaa attaaaattg gttcattaaa
     3781 tcacatcatt acaactcatc atcctgtccg catagcggag gaagcttgct tattggatca
     3841 gttaagtgaa gggagattta ttttagggtt tagtgattgc gaaaaaaaag atgaaatgca
     3901 tttttttaat cgcccggttg aatatcaaca gcaactattt gaagagtgtt atgaaatcat
     3961 taacgatgct ttaacaacag gctattgtaa tccagataac gatttttata gcttccctaa
     4021 aatatctgta aatccccatg cttatacgcc aggcggacct cggaaatatg taacagcaac
     4081 cagtcatcat attgttgagt gggcggccaa aaaaggtatt cctctcatct ttaagtggga
     4141 tgattctaat gatgttagat atgaatatgc tgaaagatat aaagccgttg cggataaata
     4201 tgacgttgac ctatcagaga tagaccatca gttaatgata ttagttaact ataacgaaga
     4261 tagtaataaa gctaaacaag agacgcgtgc atttattagt gattatgttc ttgaaatgca
     4321 ccctaatgaa aatttcgaaa ataaacttga agaaataatt gcagaaaacg ctgtcggaaa
     4381 ttatacggag tgtataactg cggctaagtt ggcaattgaa aagtgtggtg cgaaaagtgt
     4441 attgctgtcc tttgaaccaa tgaatgattt gatgagccaa aaaaatgtaa tcaatattgt
     4501 tgatgataat attaagaagt accacatgga atatacctaa tagatttcga gttgcagcga
     4561 ggcggcaagt gaacgaatcc ccaggagcat agataactat gtgactgggg tgagtgaaag
     4621 cagccaacaa agcagcagct tgaaagatga agggtataaa agagtatgac agcagtgctg
     4681 ccatactttc taatattatc ttgaggagta aaacaggtat gacttcatat gttgataaac
     4741 aagaaattac agcaagctca gaaattgatg atttgatttt ttcgagcgat ccattagtgt
     4801 ggtcttacga cgagcaggaa aaaatcagaa agaaacttgt gcttgatgca tttcgtaatc
     4861 attataaaca ttgtcgagaa tatcgtcact actgtcaggc acacaaagta gatgacaata
     4921 ttacggaaat tgatgacata cctgtattcc caacatcggt ttttaagttt actcgcttat
     4981 taacttctca ggaaaacgag attgaaagtt ggtttaccag tagcggcacg aatggtttaa
     5041 aaagtcaggt ggcgcgtgac agattaagta ttgagagact cttaggctct gtgagttatg
     5101 gcatgaaata tgttggtagt tggtttgatc atcaaataga attagtcaat ttgggaccag
     5161 atagatttaa tgctcataat atttggttta aatatgttat gagtttggtg gaattgttat
     5221 atcctacgac atttaccgta acagaagaac gaatagattt tgttaaaaca ttgaatagtc
     5281 ttgaacgaat aaaaaatcaa gggaaagatc tttgtcttat tggttcgcca tactttattt
     5341 atttactctg ccattatatg aaagataaaa aaatctcatt ttctggagat aaaagccttt
     5401 atatcataac cggaggcggc tggaaaagtt acgaaaaaga atctctgaaa cgtgatgatt
     5461 tcaatcatct tttatttgat actttcaatc tcagtgatat tagtcagatc cgagatatat
     5521 ttaatcaagt tgaactcaac acttgtttct ttgaggatga aatgcagcgt aaacatgttc
     5581 cgccgtgggt atatgcgcga gcgcttgatc ctgaaacgtt gaaacctgta cctgatggaa
     5641 cgccggggtt gatgagttat atggatgcgt cagcaaccag ttatccagca tttattgtta
     5701 ccgatgatgt cgggataatt agcagagaat atggtaagta tcccggcgtg ctcgttgaaa
     5761 ttttacgtcg cgtcaatacg aggacgcaga aagggtgtgc tttaagctta accgaagcgt
     5821 ttgatagttg atgacctgca ggtggctgct gaacccccag ccggaactga ccccacaagg
     5881 ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac caggccgctg
     5941 cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac gcgggtggaa
     6001 tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc ccggtgcgag
     6061 ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt ctcccatatg
     6121 aatttcgtgt agtggtcgcc agcaaacagc acgacgattt cctcgtcgat caggacctgg
     6181 caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag cgacaccgat
     6241 tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag gcgcgacagg
     6301 cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc ctggcaaagc
     6361 tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta ctccaacacc
     6421 tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta ggtgatcttc
     6481 acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg gattttcttg
     6541 ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg catcgtgtcc
     6601 ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg cttcgtgtgt
     6661 ttcagcaacg cggcctgctt ggcctcgctg acctgttttg ccaggtcctc gccggcggtt
     6721 tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt cgccaaacct
     6781 gccgcctcct gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc gggcagggca
     6841 gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg gaccatcgag
     6901 ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc gatggtttcg
     6961 gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt ccggtcaaac
     7021 gtccgattca ttcaccctcc ttgcgggatt gccccggaat tccccggatc gatccgtcga
     7081 tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca
     7141 gggcttccca accttaccag agggcgcccc agctggcaat tccggttcgc ttgctgtcca
     7201 taaaaccgcc cagtctagct atcgccatgt aagcccactg caagctacct gctttctctt
     7261 tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca
     7321 ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt tagcagccct tgcgccctga
     7381 gtgcttgcgg cagcgtgaag ctgacggatc gatccgggga attaattcac ccccgaacac
     7441 gagcacggca cccgcgacca ctatgccaag aatgcccaag gtaaaaattg ccggccccgc
     7501 catgaagtcc gtgaatgccc cgacggccga agtgaagggc aggccgccac ccaggccgcc
     7561 gccctcactg cccggcacct ggtcgctgaa tgtcgatgcc agcacctgcg gcacgtcaat
     7621 gcttccgggc gtcgcgctcg ggctgatcgc ccatcccgtt actgccccga tcccggcaat
     7681 ggcaaggact gccagcgctg ccatttttgg ggtgaggccg ttcgcggccg aggggcgcag
     7741 cccctggggg gatgggaggc ccgcgttagc gggccgggag ggttcgagaa gggggggcac
     7801 cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa acaaggttta
     7861 taaatattgg tttaaaagca ggttaaaaga caggttagcg gtggccgaaa aacgggcgga
     7921 aacccttgca aatgctggat tttctgcctg tggacagccc ctcaaatgtc aataggtgcg
     7981 cccctcatct gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct catctgtcag
     8041 tagtcgcgcc cctcaagtgt caataccgca gggcacttat ccccaggctt gtccacatca
     8101 tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct ggccagctcc
     8161 acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg ggtgagtcgg
     8221 cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt tccgcgaggt
     8281 atccacaacg ccggcggccg cggtgtctcg cacacggctt cgacggcgtt tctggcgcgt
     8341 ttgcagggcc atagacggcc gccagcccag cggcgagggc aaccagcccg gtgagcgtcg
     8401 gaaaggcgct ggaagccccg tagcgacgcg gagaggggcg agacaagcca agggcgcagg
     8461 ctcgatgcgc agcacgacat agccggttct cgcaaggacg agaatttccc tgcggtgccc
     8521 ctcaagtgtc aatgaaagtt tccaacgcga gccattcgcg agagccttga gtccacgcta
     8581 gatccgtcaa ttccgggatc tatctcatct gcgcaaggca gaacgtgaag acggccgccc
     8641 tggacctcgc ccgcgagcgc caggcgcacg aggccggcgc gcggacccgc gccacggccc
     8701 acgagcggac gccgcagcag gagcgccaga aggccgccag agaggccgag cgcggccgtg
     8761 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     8821 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     8881 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     8941 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     9001 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     9061 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     9121 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     9181 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     9241 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     9301 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     9361 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     9421 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     9481 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     9541 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     9601 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     9661 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     9721 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     9781 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     9841 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     9901 gccactgtcg actacgccat catggcgaca gcgcctttcc tttgggttct ctatatcggg
     9961 cggatcgtgg ccggcatcac cggggcgact ggggcggtag ccggcgctta tattgccgat
    10021 atcactgatg gcgatgagcg cgcgcggcac ttcggcttca tgagcgcctg tttcgggttc
    10081 gggatggtcg cgggacctgt gctcggtggg ctgatgggcg gtttctcccc ccacgctccg
    10141 ttcttcgccg cggcagcctt gaacggcctc aatttcctga cgggctgttt ccttttgccg
    10201 gagtcgcaca aaggcgaacg ccggccgtta cgccgggagg ctctcaaccc gctcgcttcg
    10261 ttccggtggg cccggggcat gaccgtcgtc gccgccctga tggcggtctt cttcatcatg
    10321 caacttgtcg gacaggtgcc ggccgcgctt tgggtcattt tcggcgagga tcgctttcac
    10381 tgggacgcga ccacgatcgg catttcgctt gccgcatttg gcattctgca ttcactcgcc
    10441 caggcaatga tcaccggccc tgtagccgcc cggctcggcg aaaggcgggc actcatgctc
    10501 ggaatgattg ccgacggcac aggctacatc ctgcttgcct tcgcgacacg gggatggatg
    10561 gcgttcccga tcatggtcct gcttgcttcg ggtggcatcg gaatgccggc gctgcaagca
    10621 atgttgtcca ggcaggtgga tgaggaacgt caggggcagc tgcaaggctc actggcggcg
    10681 ctcaccagcc tgacctcgat cgtcggaccc ctcctcttca cggcgatcta tgcggcttct
    10741 ataacaacgt ggaacgggtg ggcatggatt gcaggcgctg ccctctactt gctctgcctg
    10801 ccggcgctgc gtcgcgggct ttggagcggc gcagggcaac gagccgatcg ctgatcgtgg
    10861 aaacgatagg ccggaattga cggatctatc tcatctgcgc aaggcagaac gtgaagacgg
    10921 ccgccctgga cctcgcccgc gagcgccagg cgcacgaggc cggcgcgcgg acccgccgcg
    10981 gcccacgagc ggacgccgca gcaggagcgc cagaaggccg ccagagaggc cgagcgcggc
    11041 gtgaggcttg gacgctaggg cagggcatga aaaagcccgt agggggcgct acggggctct
    11101 gacgcggtgg aaagggggag gggatgttgt ctacatggct ctgctgtagt gagtgggttg
    11161 cgctccggga gcggtcctga tcccccgcag aaaaaaagga tctcaagaag atcctttgat
    11221 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    11281 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    11341 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    11401 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    11461 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    11521 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    11581 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    11641 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    11701 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    11761 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    11821 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    11881 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    11941 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga
    12001 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    12061 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    12121 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    12181 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    12241 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    12301 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    12361 gccacctggg tcgatcgacg gatcttttcc gctgcataac cctgcttcgg ggtcattata
    12421 gcgatttttt cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt
    12481 cgtgtagact ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc
    12541 ccacccgcga gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac
    12601 gggaatcctg ctctgcgagg ctggccgata agctctaaga aaccattatt atcatgacat
    12661 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaatta attccaatac
    12721 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    12781 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    12841 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    12901 aacaatttca cacaggaaac agctatgacc atgattacgc caagctgatc ccccgatgtg
    12961 tagcagtggc ggaccatata ggcagatcag aaggcgcggt tctcctacat gttcgccggt
    13021 gaacgcgttg aggaagccgg gcagtgcctc ggcaaaatcc ttgcgtgtag acaagacatc
    13081 tgcgtagcag ttgtcctcaa caacgatgtc gaaatccaaa tcggagtgct catcgagtcc
    13141 tccgtgaacg taagagccgc cgatcagaag agcgcggaag cgaacatcgg aagcgaccgc
    13201 atcgcggatg cggttcaaga aagttgcatg agcttgtgga agtgtgctga gcataaatga
    13261 ttctcctagc tgttctttgg gtaagtacgc catcaggacg ttgtgagtgg cgcgattttt
    13321 agcggctgaa atcagccctt gagcctgtcg gcaagtcgcg tcatgaggtc catgcgctca
    13381 tgcaggatcg ccacgaccaa cgcgggttcg cccgcacgcg gcaggcaaaa aacgtagtgg
    13441 tgttcgcagc gggccatccg cagcgcggga aagagttcgc tcatgtcctt aaacgggcct
    13501 tcgccggcgg caagcctggc tatgccctgt tccagcttag cgatatagcg gcgcacctgc
    13561 gccgcgcccc actcccggcg cgtgtagcgg atgatgccgc gtagatcggc ttcggcctca
    13621 gccgtgagga tgtaggccgt caagcgcgat ccccgctgag ttcttcatca agaatttcgc
    13681 cgacgctctt ggtggacacc ttgccggcaa gcccatcgtt gatgcggttc cccagcatgg
    13741 ttttcagttc ctgccatgcc tgatcggcat cagcgtcacc ggggaacaga cgttcgaggg
    13801 cgtattgctt aatggtcttg ccctgcaagg cggccagggc tttcaggctc tggtgctgct
    13861 ggtccgtcat gtcgattgtc aggcggctca ttggataacc tccataaaat acacgtaacc
    13921 acattagcac atatgtgggc gtgaggctac agcgcgaggc gcattaaggt cgggaaaatg
    13981 cgctaggcgc atttaaattg cgtattgctg taatgcgcca tgccggctag actaggccca
    14041 aatgggtata cccaatttga ccaaggggga cgcgatgagg gcggccaagc actaccgaca
    14101 acttctatcc atcgacttca acatcgaggc gctggccttc gtgcctggac ccgacggcac
    14161 acgcggccgg cgcatccacg tcctggggcg cgaggtccgc gaccggcccg gcctggtcga
    14221 gtacctttcg ccggcgttcg gctcgcgggt ggcgctggac ggctactgca aggccaattt
    14281 cgatgcagtg ctgcacctgg cgtaccccga tcatcagcaa tggggccacg catgaagcgc
    14341 cgaagctacg ccatgctgcg cgccgctgcc gcgctggccg tcctggtcgt tgcctcgccg
    14401 gcatgggccg agctgcgcgg cgaggtcgtg cgcatcatcg acggcgacac catcgacgtg
    14461 ctggtagaca agcagccggt gcgcgtgcgc ctggtggaca ttgacgcgcc ggaaaagcgg
    14521 caagccttcg gcgaacgtgc gcgccaggcg ctggccggca tggtgttccg ccggcacgtc
    14581 ctggtcgacg agaaggacac cgaccgttac ggccgcacgc tgggcaccgt gtgggtcaac
    14641 atggagctgg ccagccggcc gccgcagccg cgcaacgtca acgccgcgat ggttcaccag
    14701 ggcatggcgt gggcctatcg cttccacggc cgcgcggccg accctgaaat gctgcggctc
    14761 gaacaggagg cgcgaggcaa gcgcgtcggc ctctggtccg atccgcacgc cgtcgagccg
    14821 tggaaatggc gacgcgagag caacaaccgg agggacgaag gttgaaggtc gcccgcatct
    14881 acctgcgcgc cagtacggac gagcagaatc ttgaacgcca ggagagcctt gtagcggcca
    14941 cgcgggccgc cgggtactac gtcgccggca tctaccgcga gaaggcgtcc ggcgcacgcg
    15001 ccgaccggcc cgagctgctg cgcatgatcg cggacctgca acctggtgaa gtcgtcgttg
    15061 cggagaagat cgaccgcatc agccgcttgc cgttggccga ggccgagcgc ctggttgcgt
    15121 cgatccgggc caaaggggcc aagctggccg tgcctggcgt ggtggacctg tcggagctgg
    15181 ccgccgaggc gaacggagtg gcgaaaatcg ttctggaatc cgtccaggac atgcttttga
    15241 agctcgcctt gcagatggcc cgcgacgact acgaggatcg gcgcgagcgt caacgtcagg
    15301 gtgtccagtt ggcgaaggcc gccggccgct acaccggccg caaacgtgac gccggcatgc
    15361 acgaccgcat catcacgctt cgctccggcg gatcgagcat tgccaagacg gccaagctgg
    15421 tcggatgcag cccgagccag gtcaaacgag tgtgggcggc ctggaacgcg cagcagcaaa
    15481 aataaagccg ggcagtgccc ggcttttctc accttttcgc gtcccgcagg gccgctgcga
    15541 gcgccctacc tagatcctcg ctttccccct cggtgtagtc cggccagggc acgaagggcg
    15601 cggatgcgaa cctgttgagc aggtacgcct tcgggcagcg gtagaccacc ggcgagttcg
    15661 ccttttcatc ccaccgggcc aggatcacgt ccgcatcgca gtgcatgtcc ttcacctggt
    15721 cgcggaagaa gccgaaggcc accatgccgc tatgttcgcc gaggaacgcc agttgcttcg
    15781 cgctggcgat cgcgccgacg ccgccggcca aaaccgacgc catcacccag ccgacgaacc
    15841 agaagctggc atgcttgcgg ttgaccaccg cacgcgcagc cgcgaccagg acaacggcca
    15901 agctgccgac cagggccatg acgaccgtga tccggccgtt gtggaaagcg atgggcttgc
    15961 cagcgtccgc ttgcacggcg tcgtaaatgc tggacccgat gggcgcgcac atcagcacga
    16021 caggcagcag caccaggaac atcgtccgcg tccattgcgc gagtgccttg cggcgttcgc
    16081 cggcggcaag cgcctccatc atcggcgtga agcccaacag ggccaccgca gccgccaagc
    16141 cggcaacgat gccgcaggcg attacataca tacatcctcc ctaatgcgcc ttgcgcacgg
    16201 ttgtagtcag agtccgcggt gggggtaccg taaaagccgc ttgacaaaaa gcgttctccg
    16261 cgactattgg gtaatcgatc acccaaggag gatggtgcgg atcgtggcag tatctctttc
    16321 tgacatcgcg gaacgtgcgg gcgtctcggt aaagacggtt tccggtgcat tgcacggcgg
    16381 ctccgcccgc atgtcggacg ataagcgcca gaaggtcaag gccattgcgg aagagctcgg
    16441 ctacgtgacc aaccttgcgg cgcgtggcgt gcgacagggc tggctgccgc tggtcggcgt
    16501 tgtctccgac ggcctcatca cttcgccctt cgccaccgag atcgtacgtg gcctcgacgg
    16561 ggcggcccgc agcgccggca tggcggtctt tgcggtcaac caccgcagcg gccaatctat
    16621 cggctcggtg ctcgacgagg tgcagcagtt ccggccgcgc gccgttgctt atgccgccat
    16681 gtaccacaag gatgtcgcgc tgccggacag gttggcgggc gccgtcggcg tcatgatcaa
    16741 ctgccgagag gctaccggcc gcgtcaccgc gctggtgccg gacgaagagg gcggcgcgcg
    16801 ggagatcgtg cgttatctcc tcgtcgccgg ccgccggcgc attgccttca tcaacctgcc
    16861 aggcattctc gctggcacgc tacgcgagat ggggtttcgt gcggcgcttg gggaggcggg
    16921 catcgaccac gtcggggtgt ttccggcggt gcgccgcagc gtctacagcg accgcgcgcc
    16981 aagccttgtt gcctctcatg tggaggcgct tctcaaatcc ggtcaacggc ctgacgccat
    17041 cttgtgcggc aatgaccgcg tggcgatgga agtctatgcg gcgcttgccc gcgccggcct
    17101 tcgcatcccg gatgatatcg cggtggcaag cttcgacaac caggtcgaga ttgcctcgcg
    17161 gctggacccg ccattgacga cgatggccct gccgcaccgc gccatgggcc gccttgccat
    17221 ggagatcctc ctgaccgagc agcccgaacc gccgcatctg cataaactgc cgttccatct
    17281 ggtcgaacgg gcatccgtct gaacatcgaa tagtgccaaa caggaggaga atacgaaatg
    17341 aggattttaa tgggtaatcg tttactttcc gcactcttag cgtccgctgc cctggtcgcg
    17401 accgcgggtt tcgccgaggc gaagaccgtc atccacgtca tgcaccaggg cgatcccggc
    17461 tgggtgaagg cctatggcga cgttgccacc cgctttgagg ccgccaatcc cgatgtcgac
    17521 atcgagatga tctggatc
//
