LOCUS       sequence-170968-        3822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Alx3. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Alx3
FEATURES             Location/Qualifiers
     source          1..3822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      27..615
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     516..535
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     769..786
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    903..924
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(939..944,945..962,963..969)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    977..993
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     982..1004
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1001..1017
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1001..1017
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1035..1053
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1035..1052
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1954..1963
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1958..1967
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1997..2016)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1998..2016)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2023..2040)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2023..2039)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2032..2054)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2180..2635
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2267..2286)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2477..2498
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2713..2817
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2818..2886,2887..3678)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3036..3055)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
       61 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
      121 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
      181 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
      241 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
      301 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
      361 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
      421 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      541 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      601 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
      661 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
      721 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
      781 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      841 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      901 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      961 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     1021 gattacgcca agctatttag gtgacactat agaatactca agctatgcat ccaacgcgtt
     1081 gggagctctc ccatatggtc gacctgcagg cggccgcgaa ttcactagtg attctgtctc
     1141 atgtctccag agggcatccc ctctccatgc atgtccccat actcccactc ccatggaaat
     1201 gtggctggct tcatgggagt gccagcctcc cctgcagccc accctggcat ctattccatc
     1261 catggcttcc ctcctgccct gggagggcac agctttgagc cttctccgga tggtgactac
     1321 aagtccccaa gcctcgtttc actcaggatg aagcccaaag agccccctgg cctgctgaac
     1381 tggaccacgt gatgggttac atgggcacag actgagctgt ccacttctct tcctgctctg
     1441 acctgctcct agcccatctc tacctggaca ccaaacagca aaccctttta cctccataac
     1501 ctgggtggtt ctggaagctt ggaagaggag ctgggaccct cagcctcctg cagggaacag
     1561 gctacagggt cttatttctt aaaataggtg gggctgccta ggagacaggg acttcaaagc
     1621 aggtaggact cctccttata aaatggctcc cctcatttaa attgggctgg agaaaaggtg
     1681 acagatgccc cctgttttcc aaagactgtt tctaagtcca gggagcccag gaagatggga
     1741 ggagggtgca gactaaacag ggaggcgctc tctgtggttc ttagggctgg cctcactttt
     1801 gccttcctac caccaggggg cggtacgctc agctcctggg actgacaaga tcccagacgg
     1861 tgaaggtcag ctcaataata ggtggggggg tctcctctgt tccaagcatc cctcacttct
     1921 gccctgtgct agtctacaaa tcgaattccc gcggccgcca tggcggccgg gagcatgcga
     1981 cgtcgggccc aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca
     2041 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     2101 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     2161 cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     2221 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     2281 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     2341 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     2401 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     2461 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     2521 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     2581 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttcctga
     2641 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatcagg tggcactttt
     2701 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2761 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2821 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2881 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2941 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     3001 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     3061 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     3121 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     3181 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     3241 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3301 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3361 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3421 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3481 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3541 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3601 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3661 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3721 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3781 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag ac
//
