LOCUS       sequence-170977-        2121 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human DAGLb protein with intracellular V5 tag located on 
            the C-terminal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Singh PK, Markwick R, Lu L, Howell FV, Williams G, Doherty P
  TITLE     Assay and Inhibition of the Purified Catalytic Domain of 
            Diacylglycerol Lipase Beta.
  JOURNAL   Biochemistry. 2016 May 17;55(19):2713-21. doi: 
            10.1021/acs.biochem.6b00221. Epub 2016 May 4.
  PUBMED    27115711
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.6b00221"; journalName: "Biochemistry"; date: 
            "2016-05-17- 17"; volume: "55"; issue: "19"; pages: "2713-21"
COMMENT     Sequence Label: hDAGLb-V5
FEATURES             Location/Qualifiers
     source          1..2121
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(2018..2042)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2068..2109
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgccgggga tggtactctt cggccggcgc tgggccatcg ccagcgacga cttggtcttc
       61 ccagggttct tcgagctggt cgtgcgagtg ctgtggtgga ttggcattct gacgttgtat
      121 ctcatgcaca gaggaaagct ggactgtgct ggtggagcct tgctcagcag ttacttgatc
      181 gtcctcatga ttctcctggc agttgtcata tgtactgtgt cagccatcat gtgtgtcagc
      241 atgagaggaa cgatttgtaa ccctggaccg cggaagtcta tgtctaagct gctttacatc
      301 cgcctggcgc tgttttttcc agagatggtc tgggcctctc tgggggctgc ctgggtggca
      361 gatggtgttc agtgcgacag gacagttgta aacggcatca tcgcaaccgt cgtggtcagt
      421 tggatcatca tcgctgccac agtggtttcc attatcattg tctttgaccc tcttgggggg
      481 aaaatggctc catattcctc tgccggcccc agccacctgg atagtcatga ttcaagccag
      541 ttacttaatg gcctcaagac agcagctaca agcgtgtggg aaaccagaat caagctcttg
      601 tgctgttgca ttgggaaaga cgaccatact cgggttgctt tttcgagtac ggcagagctt
      661 ttctcaacct acttttcaga cacagatctg gtgcccagcg acattgcggc gggcctcgcc
      721 ctgcttcatc agcaacagga caatatcagg aacaaccaag agcctgccca ggtggtctgc
      781 catgccccag ggagctccca ggaagctgat ctggatgcag aattagaaaa ctgccatcat
      841 tacatgcagt ttgcagcagc ggcctatggg tggcccctct acatctacag aaaccccctc
      901 acggggctgt gcaggattgg tggtgactgc tgcagaagca gaaccacaga ctatgacttg
      961 gtcggaggcg atcagctcaa ctgtcacttc ggctccatcc tgcacaccac agggctgcag
     1021 tacagggact tcatccacgt cagcttccat gacaaggttt acgagctgcc gtttttagtg
     1081 gctctggatc acaggaaaga gtctgttgtg gtcgctgtga gggggaccat gtctctgcag
     1141 gatgtcctta cggacctgtc agcggagagt gaggtgctgg acgtggagtg tgaggtgcag
     1201 gaccgcctgg cacacaaggg tatttctcaa gctgccagat acgtttacca acgactcatc
     1261 aacgacggga ttttgagcca agccttcagc attgctcctg agtaccggct ggtcatagtg
     1321 ggccacagcc tcgggggagg ggcggccgcc ctgctggcca ccatgctcag agccgcctac
     1381 ccgcaggtca ggtgctacgc cttctcccca ccccgggggc tgtggagcaa agctctgcag
     1441 gaatattctc agagcttcat cgtgtcactc gtcctgggga aggatgtgat tcccaggctc
     1501 agtgtgacca acttggaaga tctgaagaga agaatcttgc gagtggtcgc gcactgcaat
     1561 aaacccaagt acaagatctt gctgcacggt ttgtggtacg aactgtttgg aggaaacccc
     1621 aacaacttgc ccacggagct ggacgggggc gaccaggaag tcctgacaca gcctcttctg
     1681 ggggagcaga gcctactgac gcgctggtcc ccggcctaca gcttctccag cgactcccca
     1741 ctggactctt ctcccaagta cccccctctc taccctcccg gcaggatcat ccacctgcag
     1801 gaggagggcg cctcggggcg gtttggctgc tgctctgctg ctcactatag cgccaagtgg
     1861 tcacacgaag cggaattcag caaaatactc ataggtccga agatgctcac cgaccacatg
     1921 ccagacatcc tgatgcgggc cttggacagc gtggtctccg acagagcggc ctgcgtctcc
     1981 tgtccagcac gaggggtctc cagtgtggac gtggcccacc cagctttctt gtacaaagtg
     2041 gttgatctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc
     2101 gattctacgc gtaccggtta g
//
