LOCUS       sequence-170981-       11880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression GIV in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11880)
  AUTHORS   Aznar N, Patel A, Rohena CC, Dunkel Y, Joosen LP, Taupin V, Kufareva
            I, Farquhar MG, Ghosh P
  TITLE     AMP-activated protein kinase fortifies epithelial tight junctions 
            during energetic stress via its effector GIV/Girdin.
  JOURNAL   Elife. 2016 Nov 4;5. pii: e20795. doi: 10.7554/eLife.20795.
  PUBMED    27813479
REFERENCE   2  (bases 1 to 11880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-11-4"; volume: "5. pii: e20795. doi"; pages: " 
            10.7554/eLife.20795"
COMMENT     Sequence Label: GIV-FLAG L249P (LP)
FEATURES             Location/Qualifiers
     source          1..11880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(69..89)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        87..384
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             438..1232
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(492..511)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1102..1121
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1884..2018
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1921..1940)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1975..1994
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2023..2042)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2024..2042)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2056..2072)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2056..2072)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2069..2091)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2080..2096
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2104..2110,2111..2128,2129..2134))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2149..2170
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2287..2304)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2458..3046)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2538..2557)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3217..4008,4009..4077))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3840..3859
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4078..4182)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4209..4664
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4296..4315)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4506..4527
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4790..4812
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4804..4821
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4805..4821
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        4982..5361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5362..5565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5516..5536
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             11227..11292
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    join(11303..11880,1..45)
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(11502..11521)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 tgtcctctca tgcgttgggt ccactcagta gatgcctgtt gaattgggta cgcggccagc
       61 ttggctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
      121 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
      181 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
      241 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
      301 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
      361 gaagtagtga ggaggctttt ttggaggaat tgatcagctt gggatctgat caagagacag
      421 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
      481 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
      541 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
      601 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
      661 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
      721 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
      781 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
      841 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
      901 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
      961 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     1021 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     1081 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     1141 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     1201 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     1261 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     1321 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     1381 catgctggag ttcttcgccc accccgggct cgatcccctc gcgagttggt tcagctgctg
     1441 cctgaggctg gacgacctcg cggagttcta ccggcagtgc aaatccgtcg gcatccagga
     1501 aaccagcagc ggctatccgc gcatccatgc ccccgaactg caggagtggg gaggcacgat
     1561 ggccgctttg gtcgacccgg acgggacgct cctgcgcctg atacagaacg aattgcttgc
     1621 aggcatctca tgagtgtgtc ttcccgtttt ccgcctgagg tcactgcgtg gatggagcgc
     1681 tggcgcctgc tgcgcgacgg cgagctgctc accacccact cgccaagctg gaaccgtaaa
     1741 aaggccgcgt tgctggcgtt tttccatagg ctccgccgat cataatcagc cataccacat
     1801 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     1861 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     1921 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     1981 tgtccaaact catcaatgta tcttatcatg tctggatcaa ttccctatag tgagtcgtat
     2041 taaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     2101 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     2161 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     2221 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     2281 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2341 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2401 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2461 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2521 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2581 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2641 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2701 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2761 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2821 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2881 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2941 cggaaaaaga gttggtagct cttgatccga caaacaaacc accgctggta gcggtggttt
     3001 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3061 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3121 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3181 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3241 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3301 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3361 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3421 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3481 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3541 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3601 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3661 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3721 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3781 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3841 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3901 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3961 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     4021 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     4081 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     4141 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     4201 gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4261 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4321 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4381 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     4441 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4501 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4561 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4621 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc gccattcagg
     4681 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     4741 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     4801 cgttgtaaaa cgacggccag tgccaagctg atctatacat tgaatcaata ttggcaatta
     4861 gccatattag tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg
     4921 ttgtatctat atcataatat gtacatttat attggctcat gtccaatatg accgccatgt
     4981 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     5041 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     5101 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     5161 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     5221 caagtgtatc atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc
     5281 tggcattatg cccagtacat gaccttacgg gactttccta cttggcagta catctacgta
     5341 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag
     5401 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     5461 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa
     5521 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
     5581 cagaattaag cttgcggccg catggagaac gaaattttta ctccccttct ggagcagttc
     5641 atgaccagcc ctttggtcac ttgggttaaa acgtttggac ctctggccgc aggaaatggg
     5701 accaaccttg atgaatatgt ggctttggtg gatggggtat tcttgaacca ggtcatgctc
     5761 caaattaatc ctaaattgga gagtcagaga gtaaataaaa aagtcaataa tgatgcctca
     5821 cttagaatgc acaatctatc cattttggtg agacagataa aattttatta ccaggagact
     5881 ttgcagcaat tgatcatgat gtcgttgcca aatgtcttaa tcattggcaa aaatcccttt
     5941 tctgaacaag gcacagaaga agttaaaaaa ctgcttttac ttttattggg ttgtgcagtt
     6001 cagtgtcaga aaaaagagga atttattgaa agaattcaag gtttagattt tgatacaaaa
     6061 gcagcggttg ccgcacatat tcaagaggta actcataatc aggaaaatgt gtttgacctg
     6121 caatggatgg aagtgactga tatgtcgcag gaggacatag aaccactctt gaaaaatatg
     6181 gcattgcatc taaaaagact tatagatgag agagatgaac attcagagac tatcatagaa
     6241 ctctctgaag agcgggatgg tctccatttt ctaccccatg cctcttcatc tgcacagtca
     6301 ccctgtggtt ctccaggcat gaagcgaaca gaaagtcgac aacatccgtc ggtggaactg
     6361 gcagatgcta aagccaagat aagaaggcta cggcaggaat tggaggaaaa gactgagcag
     6421 ttgttggatt gtaaacaaga acttgagcaa atggaaatag aactcaaaag gctgcaacaa
     6481 gagaacatga atttgctttc ggatgctcgc tctgccagaa tgtaccgaga tgaattagat
     6541 gcacttcgag agaaagcagt cagagtcgat aagcttgaaa gtgaagtcag cagatataaa
     6601 gagagactac atgatattga attttataag gcaagagttg aggaattaaa agaagacaat
     6661 caagttttat tagaaacaaa aaccatgttg gaagaccaac tagagggaac tcgtgctcgt
     6721 tccgataaat tacatgaatt agaaaaagag aacttacaac tgaaagctaa acttcatgat
     6781 atggaaatgg aacgagatat ggatagaaaa aagattgaag aattaatgga agaaaatatg
     6841 actttggaaa tggcacagaa acaaagtatg gatgaatcat tacatcttgg ctgggaactg
     6901 gaacagatat ccagaactag tgaactttcc gaagcacccc agaaatccct gggccatgag
     6961 gtgaatgagt tgacatcaag tagattattg aagctagaga tggaaaatca aagtttgaca
     7021 aaaaccgtgg aagagcttcg gactactgtg gattctgtgg aaggcaatgc ttccaaaatc
     7081 ctgaaaatgg aaaaagaaaa tcaaaggctc agtaaaaagg ttgagattct tgaaaatgag
     7141 attgttcaag aaaagcaaag tcttcagaat tgtcagaatt taagcaagga tctaatgaag
     7201 gagaaagctc agcttgaaaa aacaatagaa acactgagag aaaattcaga gagacagatt
     7261 aagatactgg aacaggaaaa tgaacatctg aatcaaacag tgtcttcctt aaggcagcgg
     7321 tcccagataa gtgcagaagc aagagtgaaa gacattgaaa aagaaaacaa aattcttcat
     7381 gaatctatca aagaaacaag tagcaagcta agcaagattg aatttgaaaa aagacaaatt
     7441 aaaaaagaat tggaacatta taaagaaaaa ggagaacgag ctgaagaact tgaaaatgaa
     7501 ttgcatcatc ttgaaaaaga aaatgaatta ttacagaaaa aaataactaa tttaaaaatt
     7561 acttgtgaaa aaattgaggc cttagaacaa gaaaattcag agctagaaag agaaaataga
     7621 aaattaaaaa aaacattgga tagctttaaa aatctgacct ttcagttaga atccctagaa
     7681 aaagagaatt cccaacttga tgaggaaaac ttagaactgc gaaggaatgt agaatctttg
     7741 aagtgtgcaa gcatgaaaat ggctcagcta cagctagaaa acaaagaact ggaaagtgaa
     7801 aaagagcaac ttaagaaggg tttggagctc ctgaaagcat ctttcaagaa aacagaacgc
     7861 ttagaagtta gctaccaggg tttagatata gaaaatcaaa gactgcaaaa aactttagag
     7921 aacagcaata aaaaaatcca gcaattagag agtgaactac aagacttaga gatggaaaat
     7981 caaacattgc agaaaaacct agaagaacta aaaatatcta gcaaaagact agaacagctg
     8041 gaaaaagaaa ataaatcatt agagcaagag acttctcaac tggaaaagga taagaaacaa
     8101 ttggagaagg aaaataagag actccgacaa caagcagaaa ttaaagatac cacattagaa
     8161 gaaaataatg tgaagattgg aaatttggaa aaagaaaaca aaaccctatc caaagaaatt
     8221 ggtatatata aagaatcttg tgtccgtctg aaagaactag aaaaagaaaa taaggagctt
     8281 gtgaaaagag caactattga tataaaaacg ttggttacac tacgtgagga tttggtgagt
     8341 gaaaagttga agacccaaca gatgaacaat gatctcgaaa aattaactca tgagcttgag
     8401 aagatagggt taaataagga gcgactctta catgatgaac aaagtactga tgacaggtat
     8461 aaacttttgg aatcaaaatt agaatccact cttaagaagt ctcttgaaat aaaagaagaa
     8521 aaaattgctg ctttagaagc tcgattagaa gaatccacga attataacca gcaattgcgc
     8581 caagaactta aaacagtgaa aaaaaattat gaagctctca aacagagaca agatgaggaa
     8641 aggatggtac agagctctcc tccaatatct ggtgaagaca acaaatggga gcgagaaagt
     8701 caagaaacga ctagagaact tctgaaagtt aaagacagat taattgaagt agaaagaaat
     8761 aatgctacac tgcaagcaga gaagcaagcg ttgaaaactc aactgaagca acttgagaca
     8821 cagaacaata atttgcaggc tcagattctt gcacttcaga ggcagacagt gtcattacaa
     8881 gaacagaata ccactcttca aacacagaat gccaagcttc aggttgaaaa ttccaccctt
     8941 aattcccaaa gtacctcact catgaaccag aatgcccaac tcctaatcca gcagtcttcc
     9001 ttagaaaatg aaaatgaatc tgtaatcaaa gagcgagaag acctaaaatc tctctatgat
     9061 tctctgatca aagatcatga aaagctggaa cttcttcatg aacgtcaggc ttcagagtat
     9121 gaatctctta tctctaaaca tggaactctg aagtctgccc acaaaaatct tgaggtggaa
     9181 catagagacc ttgaagaccg ttacaatcag ttattaaaac agaaaggaca gttggaagat
     9241 ttggaaaaaa tgctcaaagt agaacaggaa aaaatgctgc ttgaaaataa aaatcatgaa
     9301 acagtagctg cagaatacaa gaaactttgt ggtgaaaatg ataggctgaa tcatacctat
     9361 agtcaacttt taaaagagac tgaagtttta caaactgacc ataaaaattt gaaaagtctt
     9421 ctgaataatt ccaaactgga acaaacaaga ttagaagctg aattttcaaa actaaaggaa
     9481 caataccaac aattggatat tacatcaacc aagctgaata accagtgtga gttgctaagc
     9541 caacttaaag gaaatttaga agaagaaaat cggcatctac tagatcaaat tcagacatta
     9601 atgctacaga acagaacact tttggagcag aatatggaaa gcaaggatct ttttcatgtt
     9661 gaacaaagac agtacattga taagttaaat gaattaagac gtcagaagga gaaactagaa
     9721 gagaaaatta tggatcaata caaattttat gacccatctc ctcctagaag gagaggcaac
     9781 tggattactc taaaaatgag aaaattgata aagtctaaga aagatattaa tcgggaacgc
     9841 cagaaatctc taacattaac acccacccgc tcagactcca gtgaaggatt tcttcagctc
     9901 cctcatcaag acagtcaaga tagttcttca gtaggttcaa actctttaga agatggccag
     9961 accttgggga ccaagaaaag cagcatggtt gcactgaaaa gactgccctt tttgaggaac
    10021 agaccgaagg ataaagacaa aatgaaggcc tgctaccgtc gttccatgtc catgaatgac
    10081 ctggtgcagt ccatggtcct agcaggacag tggacaggta gtactgagaa tttggaggtt
    10141 cctgatgata tttcaacggg taaaaggaga aaagaattgg gagctatggc cttctctact
    10201 acagccatca acttttcaac tgtcaactct tctgcaggct tcagatccaa gcagttggtt
    10261 aataataaag atactacatc ctttgaagac ataagtccac aaggtgttag tgatgattct
    10321 agtacgggat caagagttca tgcttcaaga ccagccagcc ttgatagtgg cagaacatcc
    10381 actagcaata gcaataataa tgcttcacta catgaagtca aagcaggtgc agttaataac
    10441 caaagcaggc cacaaagcca cagcagtgga gaatttagcc tgcttcatga ccatgaggct
    10501 tggtccagca gtggtagcag tccaatccag tacttgaaaa gacagaccag atcaagccca
    10561 gtgctccagc acaaaatatc tgaaacactg gagagtcgac atcacaagat caaaactggt
    10621 tcccctggaa gtgaagttgt tactctacaa cagtttttgg aagaaagcaa taagcttacc
    10681 tcagtacaga taaagtcctc aagtcaagag aatcttttag atgaagtaat gaaaagtttg
    10741 tctgtctctt ctgacttttt gggaaaagac aaaccagtta gctgtggtct ggccaggtca
    10801 gtaagtggaa aaaccccagg ggacttctat gatagacgga caactaagcc tgagtttttg
    10861 agacctggtc ctcgaaaaac tgaagatacc tacttcatta gttctgcggg aaaacctaca
    10921 ccaggcactc aaggaaaaat aaaattagta aaagaatctt ctctgtcacg acaatcaaaa
    10981 gatagtaacc cttatgcaac tttacctcgt gcaagcagcg tgatctcaac tgccgaagga
    11041 actacacgaa ggacaagcat ccatgatttt ttgaccaagg acagtagact gcctatatca
    11101 gttgattcac caccagctgc tgctgacagc aacaccactg cagcatctaa tgtggacaaa
    11161 gtacaagaaa gcagaaattc aaaaagcagg tctagggagc aacaaagctc ctcaggatcc
    11221 cgggctgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
    11281 gacgatgaca agtagtgatc ccgggtggca tccctgtgac ccctccccag tgcctctcct
    11341 ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat
    11401 cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag
    11461 caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg
    11521 gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct
    11581 cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt
    11641 tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta
    11701 atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac
    11761 tgctcccttc cctgtccttc tgattttaaa ataactatac cagcaggagg acgtccagac
    11821 acagcatagg ctacctggcc atgcccaacc ggtgggacat ttgagttgct tgcttggcac
//
