LOCUS       sequence-170987-        9809 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Repressilator with triple reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Potvin-Trottier L, Lord ND, Vinnicombe G, Paulsson J
  TITLE     Synchronous long-term oscillations in a synthetic gene circuit.
  JOURNAL   Nature. 2016 Oct 12;538(7626):514-517. doi: 10.1038/nature19841.
  PUBMED    27732583
REFERENCE   2  (bases 1 to 9809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-10-12- 12"; volume: "538"; issue: "7626"; 
            pages: "514-517"
COMMENT     Sequence Label: pLPT107
FEATURES             Location/Qualifiers
     source          1..9809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..714
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      744..830
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        969..1073
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1074..1142,1143..1934)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1292..1311)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      1964..2058
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2550..2772
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             2820..3770
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(4308..4394)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(4466..5176)
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     RBS             5183..5194
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(join(5204..5229,5230..5235,5236..5252,5253..5258,
                     5259..5277))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    5234..5252
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5259..5277
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(5308..5394)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(5459..6538)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     6500..6519
                     /label=LacI-R
                     /note="LacI, reverse primer"
     RBS             6556..6567
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(6674..6760)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(6808..7428)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     7336..7355
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     RBS             7435..7446
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    7488..7504
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    7511..7527
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(7645..7663,7664..7669,7670..7686,7687..7692,
                     7693..7718)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    7645..7663
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7670..7688
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             7728..7739
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             7748..8461
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             7748..8461
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      8522..8608
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     8626..8643
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8627..8643
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9711..9727
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    9734..9750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             9792..9803
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
       61 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      121 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      181 gtcactactc tcacttgggg tgttcaatgc tttgcaagat acccagatca tatgaaacag
      241 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      301 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      361 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      421 ttggaataca actacatctc agacaatgta tacatcacgg cagacaaaca aaagaatgga
      481 atcaaagcta acttcaaaat tagacacaac attgaagatg gaggcgttca actagcagac
      541 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      601 ctgtccacac aatctaagct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      661 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataagct
      721 taattagctg agtctagagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      781 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgccct
      841 agacccgggg tgtaggctgg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      901 cggttatcca cagaatcagg ggataacgca ggaaagagac gtcaggtggc acttttcggg
      961 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1021 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1081 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1141 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1201 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1261 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     1321 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1381 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1441 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1501 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1561 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1621 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1681 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1741 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1801 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1861 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1921 ttaagcattg gtaactgtca gaccaagttt acgagctcgc ttggactcct gttgatagat
     1981 ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg
     2041 ttttttattg gtgagaatcc aagcactagg gacagtaaga cgggtaagcc tgttgatgat
     2101 accgctgcct tactgggtgc attagccagt ctgaatgacc tgtcacggga taatccgaag
     2161 tggtcagact ggaaaatcag agggcaggaa ctgctgaaca gcaaaaagtc agatagcacc
     2221 acatagcaga cccgccataa aacgccctga gaagcccgtg acgggctttt cttgtattat
     2281 gggtagtttc cttgcatgaa tccataaaag gcgcctgtag tgccatttac ccccattcac
     2341 tgccagagcc gtgagcgcag cgaactgaat gtcacgaaaa agacagcgac tcaggtgcct
     2401 gatggtcgga gacaaaagga atattcagcg atttgcccga gcttgcgagg gtgctactta
     2461 agcctttagg gttttaaggt ctgttttgta gaggagcaaa cagcgtttgc gacatccttt
     2521 tgtaatactg cggaactgac taaagtagtg agttatacac agggctggga tctattcttt
     2581 ttatcttttt ttattctttc tttattctat aaattataac cacttgaata taaacaaaaa
     2641 aaacacacaa aggtctagcg gaatttacag agggtctagc agaatttaca agttttccag
     2701 caaaggtcta gcagaattta cagataccca caactcaaag gaaaaggact agtaattatc
     2761 attgactagc ccatctcaat tggtatagtg attaaaatca cctagaccaa ttgagatgta
     2821 tgtctgaatt agttgttttc aaagcaaatg aactagcgat tagtcgctat gacttaacgg
     2881 agcatgaaac caagctaatt ttatgctgtg tggcactact caaccccacg attgaaaacc
     2941 ctacaaggaa agaacggacg gtatcgttca cttataacca atacgctcag atgatgaaca
     3001 tcagtaggga aaatgcttat ggtgtattag ctaaagcaac cagagagctg atgacgagaa
     3061 ctgtggaaat caggaatcct ttggttaaag gctttgagat tttccagtgg acaaactatg
     3121 ccaagttctc aagcgaaaaa ttagaattag tttttagtga agagatattg ccttatcttt
     3181 tccagttaaa aaaattcata aaatataatc tggaacatgt taagtctttt gaaaacaaat
     3241 actctatgag gatttatgag tggttattaa aagaactaac acaaaagaaa actcacaagg
     3301 caaatataga gattagcctt gatgaattta agttcatgtt aatgcttgaa aataactacc
     3361 atgagtttaa aaggcttaac caatgggttt tgaaaccaat aagtaaagat ttaaacactt
     3421 acagcaatat gaaattggtg gttgataagc gaggccgccc gactgatacg ttgattttcc
     3481 aagttgaact agatagacaa atggatctcg taaccgaact tgagaacaac cagataaaaa
     3541 tgaatggtga caaaatacca acaaccatta catcagattc ctacctacat aacggactaa
     3601 gaaaaacact acacgatgct ttaactgcaa aaattcagct caccagtttt gaggcaaaat
     3661 ttttgagtga catgcaaagt aagtatgatc tcaatggttc gttctcatgg ctcacgcaaa
     3721 aacaacgaac cacactagag aacatactgg ctaaatacgg aaggatctga ggttcttatg
     3781 gctcttgtat ctatcagtga agcatcaaga ctaacaaaca aaagtagaac aactgttcac
     3841 cgttacatat caaagggaaa actgtccata tgcacagatg aaaacggtgt aaaaaagata
     3901 gatacatcag agcttttacg agtttttggt gcattcaaag ctgttcacca tgaacagatc
     3961 gacaatgtaa cagatgaaca gcatgtaaca cctaatagaa caggtgaaac cagtaaaaca
     4021 aagcaactag aacatgaaat tgaacacctg agacaacttg ttacagctca acagtcacac
     4081 atagacagcc tgaaacaggc gatgctgctt atcgaatcaa agctgccgac aacacgggag
     4141 ccagtgacgc ctcccgtggg gaaaaaatca tggcaattct ggaagaaata gcgctttcag
     4201 ccggcaaacc ggctgaagcc ggatctgcga ttctgataac aaactagcaa caccagaaca
     4261 gcccgtttgc gggcagcaaa acccgtaccc taggtctagg gcggcggatt tgtcctactc
     4321 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc
     4381 tttcgtttta tttgatgcct ctagcacgcg taccatggga tcctctagat caagctgcta
     4441 aagcgtagtt ttcgtcgttt gctgcgccaa acgtctcttc aggccactga ctagcgataa
     4501 ctttccccac aacggaacaa ctctcattgc atgggatcat tgggtactgt gggtttagtg
     4561 gttgtaaaaa cacctgaccg ctatccctga tcagtttctt gaaggtaaac tcatcacccc
     4621 caagtctggc tatgcagaaa tcacctggct caacagcctg ctcagggtca acgagaatta
     4681 acattccgtc aggaaagctt ggcttggagc ctgttggtgc ggtcatggaa ttaccttcaa
     4741 cctcaagcca gaatgcagaa tcactggctt ttttggttgt gcttacccat ctctccgcat
     4801 cacctttggt aaaggttcta agctcaggtg agaacatccc tgcctgaaca tgagaaaaaa
     4861 cagggtactc atactcactt ctaagtgacg gctgcatact aaccgcttca tacatctcgt
     4921 agatttctct ggcgattgaa gggctaaatt cttcaacgct aactttgaga atttttgcaa
     4981 gcaatgcggc gttataagca tttaatgcat tgatgccatt aaataaagca ccaacgcctg
     5041 actgccccat ccccatcttg tctgcgacag attcctggga taagccaagt tcatttttct
     5101 ttttttcata aattgcttta aggcgacgtg cgtcctcaag ctgctcttgt gttaatggtt
     5161 tcttttttgt gctcatggta cctttctcct ctttaatgaa ttcggtcagt gcgtcctgct
     5221 gatgtgctca gtatctctat cactgatagg gatgtcaatc tctatcactg atagggactc
     5281 gacctgcagc taggtctagg gcggcggatt tgtcctactc aggagagcgt tcaccgacaa
     5341 acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct
     5401 ctagactcag ctaattaagc ttttaagctg ctaaagcgta gttttcgtcg tttgctgcct
     5461 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     5521 ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg
     5581 gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc
     5641 tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg
     5701 agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg cgcagcccgg
     5761 actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag
     5821 tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc
     5881 agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc
     5941 cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct
     6001 ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa
     6061 taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc
     6121 aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac
     6181 tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt
     6241 ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga
     6301 caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact
     6361 gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg
     6421 cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa
     6481 cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat
     6541 tcaccatatg gtacctttct cctctttaat gaattctagt acatgcaacc attatcaccg
     6601 ccagaggtaa aatagtcaac acgcacggtg ttagatattt atcctcgacc tgcagctagg
     6661 tctagggcgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     6721 gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctctag attaagctgc
     6781 taaagcgtag ttttcgtcgt ttgctgcaga cccactttca catttaagtt gtttttctaa
     6841 tccgcatatg atcaattcaa ggccgaataa gaaggctggc tctgcacctt ggtgatcaaa
     6901 taattcgata gcttgtcgta ataatggcgg catactatca gtagtaggtg tttccctttc
     6961 ttctttagcg acttgatgct cttgatcttc caatacgcaa cctaaagtaa aatgccccac
     7021 agcgctgagt gcatataatg cattctctag tgaaaaacct tgttggcata aaaaggctaa
     7081 ttgattttct agagtttcat actgtttttc tgtaggccgt gtacctaaat gtacttttgc
     7141 tccatcgcga tgacttagta aagcacatct aaaactttta gcgttattac gtaaaaaatc
     7201 ttgccagctt tccccttcta aagggcaaaa gtgagtatgg tgcctatcta acatctcaat
     7261 ggctaaggcg tcgagcaaag cccgcttatt ttttacatgc caatacaatg taggctgctc
     7321 tacacctagc ttctgggcga gtttacgggt tgttaaacct tcgattccga cctcattaag
     7381 cagctctaat gcgctgttaa tcactttact tttatctaat ctggacatgg tacctttctc
     7441 ctctttaatg aattcggtca gtgcgtcctg ctgatgtgct cagtatcttg ttatccgctc
     7501 acaatgtcaa ttgttatccg ctcacaattc tcgaggtgaa gacgaaaggg cctcgtgata
     7561 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgta ctcgagcacg
     7621 aggccctttc gtcttcacct cgagtcccta tcagtgatag agattgacat ccctatcagt
     7681 gatagagata ctgagcacat cagcaggacg cactgaccga attcattaaa gaggagaaag
     7741 gtaccgcatg agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
     7801 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
     7861 atacggaaaa cttaccctta aattgatttg cactactgga aaactacctg ttccatggcc
     7921 aacacttgtc actactttgg gttatggtgt tcaatgcttt gcgagatacc cagatcatat
     7981 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
     8041 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
     8101 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
     8161 acacaaattg gaatacaact ataactcaca caatgtatac atcacggcag acaaacaaaa
     8221 gaatggaatc aaagcgaact tcaaaattag acacaacatt gaagatggag gtgttcaact
     8281 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     8341 ccattacctg tcctaccaat ctaagctttc gaaagatccc aacgaaaaga gagaccacat
     8401 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     8461 ataagcttga tatcgaattc ctgcagcccg ggggatccca ttgtacgcgt gctagaggca
     8521 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     8581 ggtgaacgct ctcctgagta ggacaaatcc gccgccctag acctatgtaa aacgacggcc
     8641 agtgaattcg agctcgcgaa aaaaccccgc cgaagcgggg ttttttgcgt gatatcgaat
     8701 tcctgcagcc cgggggatcc cctagactct gtgccccagt ttgctaggga ggtcgcagta
     8761 tctggccaca gccacctcgt gctgctcgac gtaggtctct ttgtcggcct ccttgattct
     8821 ttccagtctt ctgtccacat agtagacgcc gggcatcttg aggttcttag cgggtttctt
     8881 ggatctgtat gtggtcttca agttgcagat caggtggccc ccgcccacga gcttcagggc
     8941 catgtcggct ctgccttcca ggccgccgtc agcggggtac agggtctcgg tggaggcctc
     9001 ccagccgagt gttttcttct gcatcacagg gccgttggat gggaagttca cccctctgat
     9061 cttgacgttg tagatgaggc agccgtcctg gaggctggtg tcctgggtag cggtcagcac
     9121 gcccccgtct tcgtatgtgg tgactctctc ccatgtgaag ccctcaggga aggactgctt
     9181 aaagaagtcg gggatgccct gggtgtggtt gatgaaggtt ttgctgccgt acatgaagct
     9241 ggtagccagg atgtcgaagg cgaaggggag agggccgccc tcgacggcct tgattctcat
     9301 ggtctgggtg ccctcgtagg gcttgccttc gccctcggat gtgcacttga agtggtggtt
     9361 gttcacggtg ccctccatgt acagcttcat gtgcatgttc tccttaatca gtgccatgtt
     9421 attttcttct cctttactaa ccatgcggta cctttctcct atttaatgaa ttcggtcagt
     9481 gcgtcctgct gatattctag tacatgcaac cattatcacc gccagaggta aaatagtcaa
     9541 cacgcacggt gttagatatt tatccaagga acgccagccg ccacgacgtt tggtggaatg
     9601 tcttttgtga cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     9661 acctataaaa ataggcgtat cacgaggccc tttcgtcttc acctcgagaa ttgtgagcgg
     9721 ataacaattg acattgtgag cggataacaa gatactgagc acatcagcag gacgcactga
     9781 ccgaattcat taaagaggag aaaggtacc
//
