LOCUS       sequence-170991-        8702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Repressilator without degradation tags, with integrated reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8702)
  AUTHORS   Potvin-Trottier L, Lord ND, Vinnicombe G, Paulsson J
  TITLE     Synchronous long-term oscillations in a synthetic gene circuit.
  JOURNAL   Nature. 2016 Oct 12;538(7626):514-517. doi: 10.1038/nature19841.
  PUBMED    27732583
REFERENCE   2  (bases 1 to 8702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-10-12- 12"; volume: "538"; issue: "7626"; 
            pages: "514-517"
COMMENT     Sequence Label: pLPT119
FEATURES             Location/Qualifiers
     source          1..8702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..714
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      744..830
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        969..1073
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1074..1142,1143..1934)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1292..1311)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      1964..2058
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2550..2772
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             2820..3770
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(4308..4394)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(4452..5165)
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     RBS             5172..5183
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(join(5193..5218,5219..5224,5225..5241,5242..5247,
                     5248..5266))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    5223..5241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5248..5266
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(5297..5383)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(5410..6492)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     6454..6473
                     /label=LacI-R
                     /note="LacI, reverse primer"
     RBS             6510..6521
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(6628..6714)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(6741..7364)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     7272..7291
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     RBS             7371..7382
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    7424..7440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    7447..7463
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(7486..7504)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(7581..7599,7600..7605,7606..7622,7623..7628,
                     7629..7654)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    7581..7599
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7606..7624
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             7664..7675
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             7684..8397
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             7684..8397
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      8458..8544
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     8562..8579
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8563..8579
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
       61 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      121 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      181 gtcactactc tcacttgggg tgttcaatgc tttgcaagat acccagatca tatgaaacag
      241 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      301 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      361 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      421 ttggaataca actacatctc agacaatgta tacatcacgg cagacaaaca aaagaatgga
      481 atcaaagcta acttcaaaat tagacacaac attgaagatg gaggcgttca actagcagac
      541 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      601 ctgtccacac aatctaagct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      661 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataagct
      721 taattagctg agtctagagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      781 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgccct
      841 agacccgggg tgtaggctgg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      901 cggttatcca cagaatcagg ggataacgca ggaaagagac gtcaggtggc acttttcggg
      961 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1021 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1081 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1141 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1201 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1261 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     1321 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1381 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1441 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1501 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1561 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1621 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1681 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1741 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1801 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1861 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1921 ttaagcattg gtaactgtca gaccaagttt acgagctcgc ttggactcct gttgatagat
     1981 ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg
     2041 ttttttattg gtgagaatcc aagcactagg gacagtaaga cgggtaagcc tgttgatgat
     2101 accgctgcct tactgggtgc attagccagt ctgaatgacc tgtcacggga taatccgaag
     2161 tggtcagact ggaaaatcag agggcaggaa ctgctgaaca gcaaaaagtc agatagcacc
     2221 acatagcaga cccgccataa aacgccctga gaagcccgtg acgggctttt cttgtattat
     2281 gggtagtttc cttgcatgaa tccataaaag gcgcctgtag tgccatttac ccccattcac
     2341 tgccagagcc gtgagcgcag cgaactgaat gtcacgaaaa agacagcgac tcaggtgcct
     2401 gatggtcgga gacaaaagga atattcagcg atttgcccga gcttgcgagg gtgctactta
     2461 agcctttagg gttttaaggt ctgttttgta gaggagcaaa cagcgtttgc gacatccttt
     2521 tgtaatactg cggaactgac taaagtagtg agttatacac agggctggga tctattcttt
     2581 ttatcttttt ttattctttc tttattctat aaattataac cacttgaata taaacaaaaa
     2641 aaacacacaa aggtctagcg gaatttacag agggtctagc agaatttaca agttttccag
     2701 caaaggtcta gcagaattta cagataccca caactcaaag gaaaaggact agtaattatc
     2761 attgactagc ccatctcaat tggtatagtg attaaaatca cctagaccaa ttgagatgta
     2821 tgtctgaatt agttgttttc aaagcaaatg aactagcgat tagtcgctat gacttaacgg
     2881 agcatgaaac caagctaatt ttatgctgtg tggcactact caaccccacg attgaaaacc
     2941 ctacaaggaa agaacggacg gtatcgttca cttataacca atacgctcag atgatgaaca
     3001 tcagtaggga aaatgcttat ggtgtattag ctaaagcaac cagagagctg atgacgagaa
     3061 ctgtggaaat caggaatcct ttggttaaag gctttgagat tttccagtgg acaaactatg
     3121 ccaagttctc aagcgaaaaa ttagaattag tttttagtga agagatattg ccttatcttt
     3181 tccagttaaa aaaattcata aaatataatc tggaacatgt taagtctttt gaaaacaaat
     3241 actctatgag gatttatgag tggttattaa aagaactaac acaaaagaaa actcacaagg
     3301 caaatataga gattagcctt gatgaattta agttcatgtt aatgcttgaa aataactacc
     3361 atgagtttaa aaggcttaac caatgggttt tgaaaccaat aagtaaagat ttaaacactt
     3421 acagcaatat gaaattggtg gttgataagc gaggccgccc gactgatacg ttgattttcc
     3481 aagttgaact agatagacaa atggatctcg taaccgaact tgagaacaac cagataaaaa
     3541 tgaatggtga caaaatacca acaaccatta catcagattc ctacctacat aacggactaa
     3601 gaaaaacact acacgatgct ttaactgcaa aaattcagct caccagtttt gaggcaaaat
     3661 ttttgagtga catgcaaagt aagtatgatc tcaatggttc gttctcatgg ctcacgcaaa
     3721 aacaacgaac cacactagag aacatactgg ctaaatacgg aaggatctga ggttcttatg
     3781 gctcttgtat ctatcagtga agcatcaaga ctaacaaaca aaagtagaac aactgttcac
     3841 cgttacatat caaagggaaa actgtccata tgcacagatg aaaacggtgt aaaaaagata
     3901 gatacatcag agcttttacg agtttttggt gcattcaaag ctgttcacca tgaacagatc
     3961 gacaatgtaa cagatgaaca gcatgtaaca cctaatagaa caggtgaaac cagtaaaaca
     4021 aagcaactag aacatgaaat tgaacacctg agacaacttg ttacagctca acagtcacac
     4081 atagacagcc tgaaacaggc gatgctgctt atcgaatcaa agctgccgac aacacgggag
     4141 ccagtgacgc ctcccgtggg gaaaaaatca tggcaattct ggaagaaata gcgctttcag
     4201 ccggcaaacc ggctgaagcc ggatctgcga ttctgataac aaactagcaa caccagaaca
     4261 gcccgtttgc gggcagcaaa acccgtaccc taggtctagg gcggcggatt tgtcctactc
     4321 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc
     4381 tttcgtttta tttgatgcct ctagcacgcg taccatggga tcctctagat caagctgcta
     4441 aagcgtagtt ttcagccaaa cgtctcttca ggccactgac tagcgataac tttccccaca
     4501 acggaacaac tctcattgca tgggatcatt gggtactgtg ggtttagtgg ttgtaaaaac
     4561 acctgaccgc tatccctgat cagtttcttg aaggtaaact catcaccccc aagtctggct
     4621 atgcagaaat cacctggctc aacagcctgc tcagggtcaa cgagaattaa cattccgtca
     4681 ggaaagcttg gcttggagcc tgttggtgcg gtcatggaat taccttcaac ctcaagccag
     4741 aatgcagaat cactggcttt tttggttgtg cttacccatc tctccgcatc acctttggta
     4801 aaggttctaa gctcaggtga gaacatccct gcctgaacat gagaaaaaac agggtactca
     4861 tactcacttc taagtgacgg ctgcatacta accgcttcat acatctcgta gatttctctg
     4921 gcgattgaag ggctaaattc ttcaacgcta actttgagaa tttttgcaag caatgcggcg
     4981 ttataagcat ttaatgcatt gatgccatta aataaagcac caacgcctga ctgccccatc
     5041 cccatcttgt ctgcgacaga ttcctgggat aagccaagtt catttttctt tttttcataa
     5101 attgctttaa ggcgacgtgc gtcctcaagc tgctcttgtg ttaatggttt cttttttgtg
     5161 ctcatggtac ctttctcctc tttaatgaat tcggtcagtg cgtcctgctg atgtgctcag
     5221 tatctctatc actgataggg atgtcaatct ctatcactga tagggactcg acctgcagct
     5281 aggtctaggg cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     5341 aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctc tagactcagc
     5401 taattaagct tactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     5461 atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt
     5521 caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag
     5581 caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg
     5641 cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc
     5701 aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc
     5761 aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc
     5821 ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag
     5881 atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa
     5941 cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc
     6001 ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc
     6061 cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt
     6121 aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc
     6181 gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga
     6241 tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc
     6301 aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag
     6361 ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt
     6421 caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt
     6481 tactggtttc acattcacca tatggtacct ttctcctctt taatgaattc tagtacatgc
     6541 aaccattatc accgccagag gtaaaatagt caacacgcac ggtgttagat atttatcctc
     6601 gacctgcagc taggtctagg gcggcggatt tgtcctactc aggagagcgt tcaccgacaa
     6661 acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct
     6721 ctagattaag ctgctaaagc ttaagaccca ctttcacatt taagttgttt ttctaatccg
     6781 catatgatca attcaaggcc gaataagaag gctggctctg caccttggtg atcaaataat
     6841 tcgatagctt gtcgtaataa tggcggcata ctatcagtag taggtgtttc cctttcttct
     6901 ttagcgactt gatgctcttg atcttccaat acgcaaccta aagtaaaatg ccccacagcg
     6961 ctgagtgcat ataatgcatt ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga
     7021 ttttcgagag tttcatactg tttttctgta ggccgtgtac ctaaatgtac ttttgctcca
     7081 tcgcgatgac ttagtaaagc acatctaaaa cttttagcgt tattacgtaa aaaatcttgc
     7141 cagctttccc cttctaaagg gcaaaagtga gtatggtgcc tatctaacat ctcaatggct
     7201 aaggcgtcga gcaaagcccg cttatttttt acatgccaat acaatgtagg ctgctctaca
     7261 cctagcttct gggcgagttt acgggttgtt aaaccttcga ttccgacctc attaagcagc
     7321 tctaatgcgc tgttaatcac tttactttta tctaatctgg acatggtacc tttctcctct
     7381 ttaatgaatt cggtcagtgc gtcctgctga tgtgctcagt atcttgttat ccgctcacaa
     7441 tgtcaattgt tatccgctca caattctcga ggtgaagacg aaagggcctc gtgatacgcc
     7501 tatttttata ggttaatgtc atgataataa tggtttctta gacgtactcg agcacgaggc
     7561 cctttcgtct tcacctcgag tccctatcag tgatagagat tgacatccct atcagtgata
     7621 gagatactga gcacatcagc aggacgcact gaccgaattc attaaagagg agaaaggtac
     7681 cgcatgagta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
     7741 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
     7801 ggaaaactta cccttaaatt gatttgcact actggaaaac tacctgttcc atggccaaca
     7861 cttgtcacta ctttgggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa
     7921 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
     7981 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
     8041 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
     8101 aaattggaat acaactataa ctcacacaat gtatacatca cggcagacaa acaaaagaat
     8161 ggaatcaaag cgaacttcaa aattagacac aacattgaag atggaggtgt tcaactagca
     8221 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
     8281 tacctgtcct accaatctaa gctttcgaaa gatcccaacg aaaagagaga ccacatggtc
     8341 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa
     8401 gcttgatatc gaattcctgc agcccggggg atcccattgt acgcgtgcta gaggcatcaa
     8461 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     8521 aacgctctcc tgagtaggac aaatccgccg ccctagacct atgtaaaacg acggccagtg
     8581 aattcgagct cgattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8641 tgcctaacta cggctacact agaaggacag tatttgaatt catttaagaa ggagatggat
     8701 cc
//
