LOCUS       sequence-170993-        8373 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mannitol biosensor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8373)
  AUTHORS   Pini F, East AK, Appia-Ayme C, Tomek J, Karunakaran R, 
            Mendoza-Suarez M, Edwards A, Terpolilli JJ, Roworth J, Downie JA, 
            Poole PS
  TITLE     Bacterial Biosensors for in Vivo Spatiotemporal Mapping of Root 
            Secretion.
  JOURNAL   Plant Physiol. 2017 Jul;174(3):1289-1306. doi: 10.1104/pp.16.01302. 
            Epub 2017 May 11.
  PUBMED    28495892
REFERENCE   2  (bases 1 to 8373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.16.01302"; journalName: "Plant Physiol"; date: 
            "2017-07"; volume: "174"; issue: "3"; pages: "1289-1306"
COMMENT     Sequence Label: pLMB705
FEATURES             Location/Qualifiers
     source          1..8373
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(196..1638)
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     primer_bind     complement(6450..6466)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6450..6466)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6463..6485)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6474..6490
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6498..6504,6505..6522,6523..6528))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6543..6564
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6689..6707)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     oriT            6752..6860
                     /note="incP origin of transfer"
     promoter        7037..7141
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7142..7210,7211..8002)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7360..7379)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgataaatat tccgccagac cagcaaaatg atccatcctg cgggcaaaac cagacgcaat
       61 aataatggca ttctttctct ttgggctgtt ttcttctggc agcgtttccc aaacatgaat
      121 ttttttattt ccttcaacac aaataacgtg gtcgatggtt ttatattttg attcattttc
      181 catacttttt acctattatg ggacaaatac aaggaactta tcttcttcca ggaatcgagt
      241 ctgttctatt tcaaccgcaa catccttagc cgtatagtta gatggccttt catgagaaat
      301 atatgtcact aatcgttgca acggtctcat tccgtcatga gatccaccaa ctcgaaatat
      361 gttattcatt cctgcttcta caatcctttc cgcacctttt aatgctaacg catctcgata
      421 tttaaatgat gactcccaag gaaaaataga tatggtttgc gtcttatttt tttgaacata
      481 aggcaatatt tgctcaatat tatcgacgtg atgaaggtac acacatctgc caagtggttg
      541 attaaattcc acacctgcat ttgactcaat aatcatccaa cgttgatgaa tatccacctc
      601 tacttttaat ccagcaaaca agctttcttt ttgaactaaa gaataggccg ccttttcatc
      661 aaaatctttt ttggcattcg gtaatatatg cgcatataga ttaagttttt ctatcaacgc
      721 taacttaaat tcctcataat gatttcccat gtaatatatg ttttgggcag aaaaacaagc
      781 tcgctgatcg taaaaacaaa catcatgagc cgcacctgtc gctgcggacg tcaaatcaac
      841 aggattatcg ataatgcaaa gactcttttt agaaccaaat ttaatcacat cagcataaga
      901 tggcgcatgc tctaccgccc aattaatcgc atctggccct ccccaagcga caataacatc
      961 cgcatgttgc ataatttctt ttgcgagtga tgtatcacct tggtggggcc aatatataac
     1021 agataaagag cgcgttatcg gatgattagg gtctacatca ataaaactta acgctaatgc
     1081 attagcggta aaaggatcgg ttgacgatgt ttttataata cactgattct tagttaaaat
     1141 tgcgcgtaat atagacatga tcccagataa tggaacatta cctgccaaca gatgtacaga
     1201 tttacctttc ggaaaagccc gaacataact ttcatcctga ggtagccatt catccatgat
     1261 atggcgagaa ccaagttcat tttctacaac atcataaagg ccgcctttag aacataaaat
     1321 catagatatc caattggcct ctagcttagc catttcttct gaatatccca tatatttttt
     1381 taagtcacga atgtatgtcc tgcgtcttga gtattcttca tttttccatc tttgccctac
     1441 cgtatagaga aaattgacaa tgttatgcaa ccgtaattcg ttatttccat tacaatcaat
     1501 aatgtttttt acatgagagt cattcaatat tggcaggtaa acactattat caccaaaatt
     1561 aatggattgc actaaatcat caccttcggg aaagatttca acctggccgt taataatgaa
     1621 tgaaattttt ttagtcatac gtatcctcca agcctgaatt cgtttaaacg gatcctacgt
     1681 tctcttccag ggtgacccat tcaaggtcga tgccgggatt cttcgccttg aaatcatccg
     1741 tcagcttctg catccggatc atgtcgccgt tgttcacggt cgcaatcgtc agcgtttcgg
     1801 ccgaagccat accggcaaac gccagtgctg agcaggcgcc cagcagaaaa gttctcaatg
     1861 tcataatctt cctcccagaa gatggggtgt gagcattcgc ttcgctcatg agcaattact
     1921 caacaaacaa caaagaatgt caatcgacaa tcgttgctgc gatgccgaag gacatggtca
     1981 tctcgttgat ttaattagaa tattttttgc tcaattcctg agcaaaaact cagcagtctc
     2041 ttcatcagtg atcagagcat tgatttgatg gccgacaacg gcagccctga tcgccttgaa
     2101 tttacgcttg cccttggcca gcccaatgac catcgatgcg tcgcgggacg ggatcggcgc
     2161 cgacgcgacg cgctcgttga tcgggttgtc gatcaacctg ccgtcgacat cgaagatcca
     2221 gccgcagatc tcgccgacag cacctccgcg catcagctcc atcatctcgt ccttctccag
     2281 aaaaccgtcg acgcagagcg gcccatcgat gccgagctcg ccgataccga cgaaggtgac
     2341 atcggcttgc gcgctcatgt taagcgtcga gcgcaccagc tgttggccat gcagcagctc
     2401 ccgctcctcg gccgaggtga cgagcaccgg cagcggcatc ggatagtgtc gcgccttcac
     2461 ggcatcggcc atgctgaaaa tgacgttgta ataggccgcc gatccgtccg gggcgatatt
     2521 gccggtcagc gaaacgatgc ggtgattggg acattcgatc gccggcagct ggtcgacggc
     2581 cgccttcagc gtgcggccgg taccgatggc aagcacgatc ggctcggccc gtttcagcca
     2641 gcgctcgatc tcggccgcgg ctgcctcggc gataccgacc gtcgtcgacg acgacgcggg
     2701 atcgctcggc accacctcca catgtttcag cccgaatttc cgccgtaatt gattgccgag
     2761 ctccaggcag gcggcgatcg gatgatcgag ccgcaccttg atcagacgct cggcgaccgc
     2821 cagcgacacc agccgctgcg ccgattgccg cgagatgccc atcgcagcgg cgatttcatc
     2881 ctgcgtacgc ccggcgacgt aatagagcca gccggcacgc gccgcatcgt cgagccgtgc
     2941 aggggtctcg gatcttttta ccatctattg cctaccgcgc aaaatgagcc ggcgtgccgc
     3001 cgccgaacat gatttgagtc tgtatcgcgc cagatcaaaa caaaattccg gcaaatgtcg
     3061 agcctgttgc aaagaggtcg gccaaataac gccatcactc aagcaaactc cgtttaggca
     3121 aaccattgca cgacgagata gatctcgagg gtacccccac cgcggactct gactacaacc
     3181 gtgcgcaagg cgcattaggg aggatgtatg tatgtaatcg cctgcggcat cgttgccggc
     3241 ttggcggctg cggtggccct gttgggcttc acgccgatga tggaggcgct tgccgccggc
     3301 gaacgccgca aggcactcgc gcaatggacg cggacgatgt tcctggtgct gctgcctgtc
     3361 gtgctgatgt gcgcgcccat cgggtccagc atttacgacg ccgtgcaagc ggacgctggc
     3421 aagcccatcg ctttccacaa cggccggatc acggtcgtca tggccctggt cggcagcttg
     3481 gccgttgtcc tggtcgcggc tgcgcgtgcg gtggtcaacc gcaagcatgc cagcttctgg
     3541 ttcgtcggct gggtgatggc gtcggttttg gccggcggcg tcggcgcgat cgccagcgcg
     3601 aagcaactgg cgttcctcgg cgaacatagc ggcatggtgg ccttcggctt cttccgcgac
     3661 caggtgaagg acatgcactg cgatgcggac gtgatcctgg cccggtggga tgaaaaggcg
     3721 aactcgccgg tggtctaccg ctgcccgaag gcgtacctgc tcaacaggtt cgcatccgcg
     3781 cccttcgtgc cctggccgga ctacaccgag ggggaaagcg aggatctagg tagggcgctc
     3841 gcagcggccc tgcgggacgc gaaaaggtga gaaaagccgg gcactgcccg gctttatttt
     3901 tgctgctgcg cgttccaggc cgcccacact cgtttgacct ggctcgggct gcatccgacc
     3961 agcttggccg tcttggcaat gctcgatccg ccggagcgaa gcgtgatgat gcggtcgtgc
     4021 atgccggcgt cacgtttgcg gccggtgtag cggccggcgg ccttcgccaa ctggacaccc
     4081 tgacgttgac gctcgcgccg atcctcgtag tcgtcgcggg ccatctgcaa ggcgagcttc
     4141 aaaagcatgt cctggacgga ttccagaacg attttcgcca ctccgttcgc ctcggcggcc
     4201 agctccgaca ggtccaccac gccaggcacg gccagcttgg cccctttggc ccggatcgac
     4261 gcaaccaggc gctcggcctc ggccaacggc aagcggctga tgcggtcgat cttctccgca
     4321 acgacgactt caccaggttg caggtccgcg atcatgcgca gcagctcggg ccggtcggcg
     4381 cgtgcgccgg acgccttctc gcggtagatg ccggcgacgt agtacccggc ggcccgcgtg
     4441 gccgctacaa ggctctcctg gcgttcaaga ttctgctcgt ccgtactggc gcgcaggtag
     4501 atgcgggcga ccttcaacct tcgtccctcc ggttgttgct ctcgcgtcgc catttccacg
     4561 gctcgacggc gtgcggatcg gaccagaggc cgacgcgctt gcctcgcgcc tcctgttcga
     4621 gccgcagcat ttcagggtcg gccgcgcggc cgtggaagcg ataggcccac gccatgccct
     4681 ggtgaaccat cgcggcgttg acgttgcgcg gctgcggcgg ccggctggcc agctccatgt
     4741 tgacccacac ggtgcccagc gtgcggccgt aacggtcggt gtccttctcg tcgaccagga
     4801 cgtgccggcg gaacaccatg ccggccagcg cctggcgcgc acgttcgccg aaggcttgcc
     4861 gcttttccgg cgcgtcaatg tccaccaggc gcacgcgcac cggctgcttg tctaccagca
     4921 cgtcgatggt gtcgccgtcg atgatgcgca cgacctcgcc gcgcagctcg gcccatgccg
     4981 gcgaggcaac gaccaggacg gccagcgcgg cagcggcgcg cagcatggcg tagcttcggc
     5041 gcttcatgcg tggccccatt gctgatgatc ggggtacgcc aggtgcagca ctgcatcgaa
     5101 attggccttg cagtagccgt ccagcgccac ccgcgagccg aacgccggcg aaaggtactc
     5161 gaccaggccg ggccggtcgc ggacctcgcg ccccaggacg tggatgcgcc ggccgcgtgt
     5221 gccgtcgggt ccaggcacga aggccagcgc ctcgatgttg aagtcgatgg atagaagttg
     5281 tcggtagtgc ttggccgccc tcatcgcgtc ccccttggtc aaattgggta tacccatttg
     5341 ggcctagtct agccggcatg gcgcattaca gcaatacgca atttaaatgc gcctagcgca
     5401 ttttcccgac cttaatgcgc ctcgcgctgt agcctcacgc ccacatatgt gctaatgtgg
     5461 ttacgtgtat tttatggagg ttatccaatg agccgcctga caatcgacat gacggaccag
     5521 cagcaccaga gcctgaaagc cctggccgcc ttgcagggca agaccattaa gcaatacgcc
     5581 ctcgaacgtc tgttccccgg tgacgctgat gccgatcagg catggcagga actgaaaacc
     5641 atgctgggga accgcatcaa cgatgggctt gccggcaagg tgtccaccaa gagcgtcggc
     5701 gaaattcttg atgaagaact cagcggggat cgcgcttgac ggcctacatc ctcacggctg
     5761 aggccgaagc cgatctacgc ggcatcatcc gctacacgcg ccgggagtgg ggcgcggcgc
     5821 aggtgcgccg ctatatcgct aagctggaac agggcatagc caggcttgcc gccggcgaag
     5881 gcccgtttaa ggacatgagc gaactctttc ccgcgctgcg gatggcccgc tgcgaacacc
     5941 actacgtttt ttgcctgccg cgtgcgggcg aacccgcgtt ggtcgtggcg atcctgcatg
     6001 agcgcatgga cctcatgacg cgacttgccg acaggctcaa gggctgattt cagccgctaa
     6061 aaatcgcgcc actcacaacg tcctgatggc gtacttaccc aaagaacagc taggagaatc
     6121 atttatgctc agcacacttc cacaagctca tgcaactttc ttgaaccgca tccgcgatgc
     6181 ggtcgcttcc gatgttcgct tccgcgctct tctgatcggc ggctcttacg ttcacggagg
     6241 actcgatgag cactccgatt tggatttcga catcgttgtt gaggacaact gctacgcaga
     6301 tgtcttgtct acacgcaagg attttgccga ggcactgccc ggcttcctca acgcgttcac
     6361 cggcgaacat gtaggagaac cgcgccttct gatctgccta tatggtccgc cactgctaca
     6421 catcggggga tcagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     6481 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     6541 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     6601 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     6661 attggaatta attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     6721 gtcatgataa taatggtttc ttagagctta tcggccagcc tcgcagagca ggattcccgt
     6781 tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc
     6841 ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa
     6901 ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta
     6961 taatgacccc gaagcagggt tatgcagcgg aaaagatccg tcgatcgacc caggtggcac
     7021 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     7081 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     7141 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     7201 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     7261 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     7321 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     7381 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7441 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     7501 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     7561 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7621 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7681 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7741 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7801 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7861 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7921 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7981 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     8041 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     8101 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8161 caaaggatct tcttgagatc ctttttttct gcgggggatc aggaccgctg ccggagcgca
     8221 acccactcac tacagcagag ccatgtagac aacatcccct ccccctttcc accgcgtcag
     8281 acgcccgtag cagcccgcta cgggcttttt catgccctgc cctagcgtcc aagcctcacg
     8341 gccgcgctcg gcctctctgg cggccttctg gcg
//
