LOCUS       sequence-170994-        3821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mannitol biosensor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Pini F, East AK, Appia-Ayme C, Tomek J, Karunakaran R, 
            Mendoza-Suarez M, Edwards A, Terpolilli JJ, Roworth J, Downie JA, 
            Poole PS
  TITLE     Bacterial Biosensors for in Vivo Spatiotemporal Mapping of Root 
            Secretion.
  JOURNAL   Plant Physiol. 2017 Jul;174(3):1289-1306. doi: 10.1104/pp.16.01302. 
            Epub 2017 May 11.
  PUBMED    28495892
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.16.01302"; journalName: "Plant Physiol"; date: 
            "2017-07"; volume: "174"; issue: "3"; pages: "1289-1306"
COMMENT     Sequence Label: pLMB705
FEATURES             Location/Qualifiers
     source          1..3821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..1115)
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     CDS             complement(1294..2277)
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             complement(2292..3374)
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
ORIGIN
        1 catcaactat caaacgcttc ggttaagctt aaagcacacc ctttctgcgt cctcgtattg
       61 acgcgacgta aaatttcaac gagcacgccg ggatacttac catattctct gctaattatc
      121 ccgacatcat cggtaacaat aaatgctgga taactggttg ctgacgcatc catataactc
      181 atcaaccccg gcgttccatc aggtacaggt ttcaacgttt caggatcaag cgctcgcgca
      241 tatacccacg gcggaacatg tttacgctgc atttcatcct caaagaaaca agtgttgagt
      301 tcaacttgat taaatatatc tcggatctga ctaatatcac tgagattgaa agtatcaaat
      361 aaaagatgat tgaaatcatc acgtttcaga gattcttttt cgtaactttt ccagccgcct
      421 ccggttatga tataaaggct tttatctcca gaaaatgaga tttttttatc tttcatataa
      481 tggcagagta aataaataaa gtatggcgaa ccaataagac aaagatcttt cccttgattt
      541 tttattcgtt caagactatt caatgtttta acaaaatcta ttcgttcttc tgttacggta
      601 aatgtcgtag gatataacaa ttccaccaaa ctcataacat atttaaacca aatattatga
      661 gcattaaatc tatctggtcc caaattgact aattctattt gatgatcaaa ccaactacca
      721 acatatttca tgccataact cacagagcct aagagtctct caatacttaa tctgtcacgc
      781 gccacctgac tttttaaacc attcgtgccg ctactggtaa accaactttc aatctcgttt
      841 tcctgagaag ttaataagcg agtaaactta aaaaccgatg ttgggaatac aggtatgtca
      901 tcaatttccg taatattgtc atctactttg tgtgcctgac agtagtgacg atattctcga
      961 caatgtttat aatgattacg aaatgcatca agcacaagtt tctttctgat tttttcctgc
     1021 tcgtcgtaag accacactaa tggatcgctc gaaaaaatca aatcatcaat ttctgagctt
     1081 gctgtaattt cttgtttatc aacatatgaa gtcatacctg ttttactcct caagataata
     1141 ttagaaagta tggcagcact gctgtcatac tcttttatac ccttcatctt tcaagctgct
     1201 gctttgttgg ctgctttcac tcaccccagt cacatagtta tctatgctcc tggggattcg
     1261 ttcacttgcc gcctcgctgc aactcgaaat ctattaggta tattccatgt ggtacttctt
     1321 aatattatca tcaacaatat tgattacatt tttttggctc atcaaatcat tcattggttc
     1381 aaaggacagc aatacacttt tcgcaccaca cttttcaatt gccaacttag ccgcagttat
     1441 acactccgta taatttccga cagcgttttc tgcaattatt tcttcaagtt tattttcgaa
     1501 attttcatta gggtgcattt caagaacata atcactaata aatgcacgcg tctcttgttt
     1561 agctttatta ctatcttcgt tatagttaac taatatcatt aactgatggt ctatctctga
     1621 taggtcaacg tcatatttat ccgcaacggc tttatatctt tcagcatatt catatctaac
     1681 atcattagaa tcatcccact taaagatgag aggaatacct tttttggccg cccactcaac
     1741 aatatgatga ctggttgctg ttacatattt ccgaggtccg cctggcgtat aagcatgggg
     1801 atttacagat attttaggga agctataaaa atcgttatct ggattacaat agcctgttgt
     1861 taaagcatcg ttaatgattt cataacactc ttcaaatagt tgctgttgat attcaaccgg
     1921 gcgattaaaa aaatgcattt catctttttt ttcgcaatca ctaaacccta aaataaatct
     1981 cccttcactt aactgatcca ataagcaagc ttcctccgct atgcggacag gatgatgagt
     2041 tgtaatgatg tgatttaatg aaccaatttt aattttctct gttaaaccga gcagaaaacc
     2101 agaaacagtc agaggagcgc cgacaacacc attatctgaa aaatgatttt catacactaa
     2161 aatctgttca aaattcaact tatcaacata ctccgttatt tcctgcatgc gaactatact
     2221 ttgttcttga acagttgttg aattgatgaa gttaaggaag aacaatccaa atttcatttc
     2281 tttctcctta gctaatataa tagcgaacgt tgtttttctt taagaaatgg catgacatca
     2341 gactggaaga gcttcatgga agcaataatt tcgtctactg ttccattagc ttcaaatcca
     2401 caacaaatat ttgatattcc tgtagcatca atgtcttttt gaattatgtc aatacattcc
     2461 tgcggcgttc ccacgggatt gatttcgtaa ctgtaatcaa tacggcgatt agtatcttta
     2521 tgtcctttta atacaaagtc acgccactgc cctttattga aatcataacc tcttgtttgg
     2581 tctgaatcat caaaaatagt cgtagcattc acataagaat cataccaatg ccccagaaat
     2641 ttccggcaaa tctctttcgc tttaattgag tcatgatcta cagatgttat atatgataag
     2701 caatggtcga tattatgaat atcgtgccca tattcttgag ccacttcatt ataaagctca
     2761 agttgtgctt tcttttcgtt agtatttata atccaactta atatcatcgg taggccaaat
     2821 tgagcagccc actcagtcgt cgaagctgat tcagccacca cataaaccgg tgcgccacct
     2881 ctgctatacg ccgcggggtt tacttttacc ttatggaact tgatatgttc attatcagct
     2941 tccatatatc cctctgtcat gccattcttt atcagcccgt accagcattc cgctaaggcg
     3001 cgactgttat tcatatctgt gccgaatacg cgaaagtcct tgttgtaaag ccctcggcaa
     3061 ataccaaacc gaaatcgtcc ttttgacatt tgatccaata aattcacatc ttcaagttgg
     3121 cgtactggat gggctgtggg aagaacaata gcggcagttc ctacattcaa ttttttagtc
     3181 gcgccaagta aatatgcagc agcgacataa gggttaccaa gcaaaccaaa ctccgtgaaa
     3241 tgatgctcca gtaaccatac ggtatcaaaa ccacactcct cagagatgcg acctaattta
     3301 accaaacgtt tcattacctc tgtttgagaa aattggggag gttggtatgt aagcaaaaag
     3361 tttccaaatt tcatagagag tccttatatt gctatttgag tgatagaata tctcaataga
     3421 ttttaagaca gagaaattgc ttgattttca atctcaattc tcattcggcg ttcattgact
     3481 gtcgcaatag ttaaatgttc aaatgacggt tcagtaatat caacatcaat atccagatga
     3541 tcattatcca tcgcgatagc ggctttcgta accgattgat aaaaattgcg caggaccact
     3601 aaattttcac tcaagtcatg cgaacttcct aacaaagaat atatcttgca tcgattacta
     3661 cgaatatttg ataacaatgt gataacttca tcttgcttga cccaattatc gttatttgca
     3721 gtaaaagcaa taaacggtat atcaagatac atcatgttat taattgtaga agctaaatct
     3781 tcccaaccaa aatcaagaca atctctcgca aagacttcag c
//
