LOCUS       sequence-170996-        5090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mannitol biosensor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Pini F, East AK, Appia-Ayme C, Tomek J, Karunakaran R, 
            Mendoza-Suarez M, Edwards A, Terpolilli JJ, Roworth J, Downie JA, 
            Poole PS
  TITLE     Bacterial Biosensors for in Vivo Spatiotemporal Mapping of Root 
            Secretion.
  JOURNAL   Plant Physiol. 2017 Jul;174(3):1289-1306. doi: 10.1104/pp.16.01302. 
            Epub 2017 May 11.
  PUBMED    28495892
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.16.01302"; journalName: "Plant Physiol"; date: 
            "2017-07"; volume: "174"; issue: "3"; pages: "1289-1306"
COMMENT     Sequence Label: pLMB705
FEATURES             Location/Qualifiers
     source          1..5090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(95..1294)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             1400..2050
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     rep_origin      2540..3251
                     /label=oriV
                     /note="incP origin of replication"
     CDS             3918..5066
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
ORIGIN
        1 tggcgctcgc gggcgaggtc cagggcggcc gtcttcacgt tctgccttgc gcagatgaga
       61 tagatccgtc aattccggcc tatcgtttcc acgatcagcg atcggctcgt tgccctgcgc
      121 cgctccaaag cccgcgacgc agcgccggca ggcagagcaa gtagagggca gcgcctgcaa
      181 tccatgccca cccgttccac gttgttatag aagccgcata gatcgccgtg aagaggaggg
      241 gtccgacgat cgaggtcagg ctggtgagcg ccgccagtga gccttgcagc tgcccctgac
      301 gttcctcatc cacctgcctg gacaacattg cttgcagcgc cggcattccg atgccacccg
      361 aagcaagcag gaccatgatc gggaacgcca tccatccccg tgtcgcgaag gcaagcagga
      421 tgtagcctgt gccgtcggca atcattccga gcatgagtgc ccgcctttcg ccgagccggg
      481 cggctacagg gccggtgatc attgcctggg cgagtgaatg cagaatgcca aatgcggcaa
      541 gcgaaatgcc gatcgtggtc gcgtcccagt gaaagcgatc ctcgccgaaa atgacccaaa
      601 gcgcggccgg cacctgtccg acaagttgca tgatgaagaa gaccgccatc agggcggcga
      661 cgacggtcat gccccgggcc caccggaacg aagcgagcgg gttgagagcc tcccggcgta
      721 acggccggcg ttcgcctttg tgcgactccg gcaaaaggaa acagcccgtc aggaaattga
      781 ggccgttcaa ggctgccgcg gcgaagaacg gagcgtgggg ggagaaaccg cccatcagcc
      841 caccgagcac aggtcccgcg accatcccga acccgaaaca ggcgctcatg aagccgaagt
      901 gccgcgcgcg ctcatcgcca tcagtgatat cggcaatata agcgccggct accgccccag
      961 tcgccccggt gatgccggcc acgatccgcc cgatatagag aacccaaagg aaaggcgctg
     1021 tcgccatgat ggcgtagtcg acagtggcgc cggccagcga gacgagcaag attggccgcc
     1081 gcccgaaacg atccgacagc gcgcccagca caggtgcgca ggcaaattgc accaacgcat
     1141 acagcgccag cagaatgcca tagtgggcgg tgacgtcgtt cgagtgaacc agatcgcgca
     1201 ggaggcccgg cagcaccggc ataatcaggc cgatgccgac agcgtcgagc gcgacagtgc
     1261 tcagaattac gatcaggggt atgttgggtt tcacgtctgg cctccggacc agcctccgct
     1321 ggtccgattg aacgcgcgga ttctttatca ctgataagtt ggtggacata ttatgtttat
     1381 cagtgataaa gtgtcaagca tgacaaagtt gcagccgaat acagtgatcc gtgccgccct
     1441 ggacctgttg aacgaggtcg gcgtagacgg tctgacgaca cgcaaactgg cggaacggtt
     1501 gggggttcag cagccggcgc tttactggca cttcaggaac aagcgggcgc tgctcgacgc
     1561 actggccgaa gccatgctgg cggagaatca tacgcattcg gtgccgagag ccgacgacga
     1621 ctggcgctca tttctgatcg ggaatgcccg cagcttcagg caggcgctgc tcgcctaccg
     1681 cgatggcgcg cgcatccatg ccggcacgcg accgggcgca ccgcagatgg aaacggccga
     1741 cgcgcagctt cgcttcctct gcgaggcggg tttttcggcc ggggacgccg tcaatgcgct
     1801 gatgacaatc agctacttca ctgttggggc cgtgcttgag gagcaggccg gcgacagcga
     1861 tgccggcgag cgcggcggca ccgttgaaca ggctccgctc tcgccgctgt tgcgggccgc
     1921 gatagacgcc ttcgacgaag ccggtccgga cgcagcgttc gagcagggac tcgcggtgat
     1981 tgtcgatgga ttggcgaaaa ggaggctcgt tgtcaggaac gttgaaggac cgagaaaggg
     2041 tgacgattga tcaggaccgc tgccggagcg caacccactc actacagcag agccatgtag
     2101 acaacatccc ctcccccttt ccaccgcgtc agacgcccgt agcagcccgc tacgggcttt
     2161 ttcatgccct gccctagcgt ccaagcctca cggccgcgct cggcctctct ggcggccttc
     2221 tggcgctcct gctgcggcgt ccgctcgtgg gccgtggcgc gggtccgcgc gccggcctcg
     2281 tgcgcctggc gctcgcgggc gaggtccagg gcggccgtct tcacgttctg ccttgcgcag
     2341 atgagataga tcccggaatt gacggatcta gcgtggactc aaggctctcg cgaatggctc
     2401 gcgttggaaa ctttcattga cacttgaggg gcaccgcagg gaaattctcg tccttgcgag
     2461 aaccggctat gtcgtgctgc gcatcgagcc tgcgcccttg gcttgtctcg cccctctccg
     2521 cgtcgctacg gggcttccag cgcctttccg acgctcaccg ggctggttgc cctcgccgct
     2581 gggctggcgg ccgtctatgg ccctgcaaac gcgccagaaa cgccgtcgaa gccgtgtgcg
     2641 agacaccgcg gccgccggcg ttgtggatac ctcgcggaaa acttggccct cactgacaga
     2701 tgaggggcgg acgttgacac ttgaggggcc gactcacccg gcgcggcgtt gacagatgag
     2761 gggcaggctc gatttcggcc ggcgacgtgg agctggccag cctcgcaaat cggcgaaaac
     2821 gcctgatttt acgcgagttt cccacagatg atgtggacaa gcctggggat aagtgccctg
     2881 cggtattgac acttgagggg cgcgactact gacagatgag gggcgcgatc cttgacactt
     2941 gaggggcaga gtgctgacag atgaggggcg cacctattga catttgaggg gctgtccaca
     3001 ggcagaaaat ccagcatttg caagggtttc cgcccgtttt tcggccaccg ctaacctgtc
     3061 ttttaacctg cttttaaacc aatatttata aaccttgttt ttaaccaggg ctgcgccctg
     3121 tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct cccggcccgc
     3181 taacgcgggc ctcccatccc cccaggggct gcgcccctcg gccgcgaacg gcctcacccc
     3241 aaaaatggca gcgctggcag tccttgccat tgccgggatc ggggcagtaa cgggatgggc
     3301 gatcagcccg agcgcgacgc ccggaagcat tgacgtgccg caggtgctgg catcgacatt
     3361 cagcgaccag gtgccgggca gtgagggcgg cggcctgggt ggcggcctgc ccttcacttc
     3421 ggccgtcggg gcattcacgg acttcatggc ggggccggca atttttacct tgggcattct
     3481 tggcatagtg gtcgcgggtg ccgtgctcgt gttcgggggt gaattaattc cccggatcga
     3541 tccgtcagct tcacgctgcc gcaagcactc agggcgcaag ggctgctaaa ggaagcggaa
     3601 cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc
     3661 tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac
     3721 atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg
     3781 ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc
     3841 aaggatctga tggcgcaggg gatcaagatc gacggatcga tccggggaat tccggggcaa
     3901 tcccgcaagg agggtgaatg aatcggacgt ttgaccggaa ggcatacagg caagaactga
     3961 tcgacgcggg gttttccgcc gaggatgccg aaaccatcgc aagccgcacc gtcatgcgtg
     4021 cgccccgcga aaccttccag tccgtcggct cgatggtcca gcaagctacg gccaagatcg
     4081 agcgcgacag cgtgcaactg gctccccctg ccctgcccgc gccatcggcc gccgtggagc
     4141 gttcgcgtcg tctcgaacag gaggcggcag gtttggcgaa gtcgatgacc atcgacacgc
     4201 gaggaactat gacgaccaag aagcgaaaaa ccgccggcga ggacctggca aaacaggtca
     4261 gcgaggccaa gcaggccgcg ttgctgaaac acacgaagca gcagatcaag gaaatgcagc
     4321 tttccttgtt cgatattgcg ccgtggccgg acacgatgcg agcgatgcca aacgacacgg
     4381 cccgctctgc cctgttcacc acgcgcaaca agaaaatccc gcgcgaggcg ctgcaaaaca
     4441 aggtcatttt ccacgtcaac aaggacgtga agatcaccta caccggcgtc gagctgcggg
     4501 ccgacgatga cgaactggtg tggcagcagg tgttggagta cgcgaagcgc acccctatcg
     4561 gcgagccgat caccttcacg ttctacgagc tttgccagga cctgggctgg tcgatcaatg
     4621 gccggtatta cacgaaggcc gaggaatgcc tgtcgcgcct acaggcgacg gcgatgggct
     4681 tcacgtccga ccgcgttggg cacctggaat cggtgtcgct gctgcaccgc ttccgcgtcc
     4741 tggaccgtgg caagaaaacg tcccgttgcc aggtcctgat cgacgaggaa atcgtcgtgc
     4801 tgtttgctgg cgaccactac acgaaattca tatgggagaa gtaccgcaag ctgtcgccga
     4861 cggcccgacg gatgttcgac tatttcagct cgcaccggga gccgtacccg ctcaagctgg
     4921 aaaccttccg cctcatgtgc ggatcggatt ccacccgcgt gaagaagtgg cgcgagcagg
     4981 tcggcgaagc ctgcgaagag ttgcgaggca gcggcctggt ggaacacgcc tgggtcaatg
     5041 atgacctggt gcattgcaaa cgctagggcc ttgtggggtc agttccggct
//
