LOCUS       sequence-171000-       17358 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Salicylic acid biosensor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17358)
  AUTHORS   Pini F, East AK, Appia-Ayme C, Tomek J, Karunakaran R, 
            Mendoza-Suarez M, Edwards A, Terpolilli JJ, Roworth J, Downie JA, 
            Poole PS
  TITLE     Bacterial Biosensors for in Vivo Spatiotemporal Mapping of Root 
            Secretion.
  JOURNAL   Plant Physiol. 2017 Jul;174(3):1289-1306. doi: 10.1104/pp.16.01302. 
            Epub 2017 May 11.
  PUBMED    28495892
REFERENCE   2  (bases 1 to 17358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.16.01302"; journalName: "Plant Physiol"; date: 
            "2017-07"; volume: "174"; issue: "3"; pages: "1289-1306"
COMMENT     Sequence Label: pLMB713
FEATURES             Location/Qualifiers
     source          1..17358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      107..818
                     /label=oriV
                     /note="incP origin of replication"
     CDS             1485..2633
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(2685..3797)
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     CDS             complement(3976..4959)
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             complement(4974..6056)
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             complement(6105..7028)
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             complement(7041..8483)
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     primer_bind     complement(12941..12957)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12941..12957)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12954..12976)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12965..12981
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12989..12995,12996..13013,13014..13019))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13034..13055
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13180..13198)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     oriT            13243..13351
                     /note="incP origin of transfer"
     promoter        13528..13632
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13633..13701,13702..14493)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13851..13870)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(15020..16219)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             16325..16975
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
ORIGIN
        1 ccgcagggaa attctcgtcc ttgcgagaac cggctatgtc gtgctgcgca tcgagcctgc
       61 gcccttggct tgtctcgccc ctctccgcgt cgctacgggg cttccagcgc ctttccgacg
      121 ctcaccgggc tggttgccct cgccgctggg ctggcggccg tctatggccc tgcaaacgcg
      181 ccagaaacgc cgtcgaagcc gtgtgcgaga caccgcggcc gccggcgttg tggatacctc
      241 gcggaaaact tggccctcac tgacagatga ggggcggacg ttgacacttg aggggccgac
      301 tcacccggcg cggcgttgac agatgagggg caggctcgat ttcggccggc gacgtggagc
      361 tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg cgagtttccc acagatgatg
      421 tggacaagcc tggggataag tgccctgcgg tattgacact tgaggggcgc gactactgac
      481 agatgagggg cgcgatcctt gacacttgag gggcagagtg ctgacagatg aggggcgcac
      541 ctattgacat ttgaggggct gtccacaggc agaaaatcca gcatttgcaa gggtttccgc
      601 ccgtttttcg gccaccgcta acctgtcttt taacctgctt ttaaaccaat atttataaac
      661 cttgttttta accagggctg cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc
      721 cccccttctc gaaccctccc ggcccgctaa cgcgggcctc ccatcccccc aggggctgcg
      781 cccctcggcc gcgaacggcc tcaccccaaa aatggcagcg ctggcagtcc ttgccattgc
      841 cgggatcggg gcagtaacgg gatgggcgat cagcccgagc gcgacgcccg gaagcattga
      901 cgtgccgcag gtgctggcat cgacattcag cgaccaggtg ccgggcagtg agggcggcgg
      961 cctgggtggc ggcctgccct tcacttcggc cgtcggggca ttcacggact tcatggcggg
     1021 gccggcaatt tttaccttgg gcattcttgg catagtggtc gcgggtgccg tgctcgtgtt
     1081 cgggggtgaa ttaattcccc ggatcgatcc gtcagcttca cgctgccgca agcactcagg
     1141 gcgcaagggc tgctaaagga agcggaacac gtagaaagcc agtccgcaga aacggtgctg
     1201 accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag
     1261 aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac
     1321 agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa
     1381 agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat caagatcgac
     1441 ggatcgatcc ggggaattcc ggggcaatcc cgcaaggagg gtgaatgaat cggacgtttg
     1501 accggaaggc atacaggcaa gaactgatcg acgcggggtt ttccgccgag gatgccgaaa
     1561 ccatcgcaag ccgcaccgtc atgcgtgcgc cccgcgaaac cttccagtcc gtcggctcga
     1621 tggtccagca agctacggcc aagatcgagc gcgacagcgt gcaactggct ccccctgccc
     1681 tgcccgcgcc atcggccgcc gtggagcgtt cgcgtcgtct cgaacaggag gcggcaggtt
     1741 tggcgaagtc gatgaccatc gacacgcgag gaactatgac gaccaagaag cgaaaaaccg
     1801 ccggcgagga cctggcaaaa caggtcagcg aggccaagca ggccgcgttg ctgaaacaca
     1861 cgaagcagca gatcaaggaa atgcagcttt ccttgttcga tattgcgccg tggccggaca
     1921 cgatgcgagc gatgccaaac gacacggccc gctctgccct gttcaccacg cgcaacaaga
     1981 aaatcccgcg cgaggcgctg caaaacaagg tcattttcca cgtcaacaag gacgtgaaga
     2041 tcacctacac cggcgtcgag ctgcgggccg acgatgacga actggtgtgg cagcaggtgt
     2101 tggagtacgc gaagcgcacc cctatcggcg agccgatcac cttcacgttc tacgagcttt
     2161 gccaggacct gggctggtcg atcaatggcc ggtattacac gaaggccgag gaatgcctgt
     2221 cgcgcctaca ggcgacggcg atgggcttca cgtccgaccg cgttgggcac ctggaatcgg
     2281 tgtcgctgct gcaccgcttc cgcgtcctgg accgtggcaa gaaaacgtcc cgttgccagg
     2341 tcctgatcga cgaggaaatc gtcgtgctgt ttgctggcga ccactacacg aaattcatat
     2401 gggagaagta ccgcaagctg tcgccgacgg cccgacggat gttcgactat ttcagctcgc
     2461 accgggagcc gtacccgctc aagctggaaa ccttccgcct catgtgcgga tcggattcca
     2521 cccgcgtgaa gaagtggcgc gagcaggtcg gcgaagcctg cgaagagttg cgaggcagcg
     2581 gcctggtgga acacgcctgg gtcaatgatg acctggtgca ttgcaaacgc tagggccttg
     2641 tggggtcagt tccggctggg ggttcagcag ccacctgcag gtcatcaact atcaaacgct
     2701 tcggttaagc ttaaagcaca ccctttctgc gtcctcgtat tgacgcgacg taaaatttca
     2761 acgagcacgc cgggatactt accatattct ctgctaatta tcccgacatc atcggtaaca
     2821 ataaatgctg gataactggt tgctgacgca tccatataac tcatcaaccc cggcgttcca
     2881 tcaggtacag gtttcaacgt ttcaggatca agcgctcgcg catataccca cggcggaaca
     2941 tgtttacgct gcatttcatc ctcaaagaaa caagtgttga gttcaacttg attaaatata
     3001 tctcggatct gactaatatc actgagattg aaagtatcaa ataaaagatg attgaaatca
     3061 tcacgtttca gagattcttt ttcgtaactt ttccagccgc ctccggttat gatataaagg
     3121 cttttatctc cagaaaatga gattttttta tctttcatat aatggcagag taaataaata
     3181 aagtatggcg aaccaataag acaaagatct ttcccttgat tttttattcg ttcaagacta
     3241 ttcaatgttt taacaaaatc tattcgttct tctgttacgg taaatgtcgt aggatataac
     3301 aattccacca aactcataac atatttaaac caaatattat gagcattaaa tctatctggt
     3361 cccaaattga ctaattctat ttgatgatca aaccaactac caacatattt catgccataa
     3421 ctcacagagc ctaagagtct ctcaatactt aatctgtcac gcgccacctg actttttaaa
     3481 ccattcgtgc cgctactggt aaaccaactt tcaatctcgt tttcctgaga agttaataag
     3541 cgagtaaact taaaaaccga tgttgggaat acaggtatgt catcaatttc cgtaatattg
     3601 tcatctactt tgtgtgcctg acagtagtga cgatattctc gacaatgttt ataatgatta
     3661 cgaaatgcat caagcacaag tttctttctg attttttcct gctcgtcgta agaccacact
     3721 aatggatcgc tcgaaaaaat caaatcatca atttctgagc ttgctgtaat ttcttgttta
     3781 tcaacatatg aagtcatacc tgttttactc ctcaagataa tattagaaag tatggcagca
     3841 ctgctgtcat actcttttat acccttcatc tttcaagctg ctgctttgtt ggctgctttc
     3901 actcacccca gtcacatagt tatctatgct cctggggatt cgttcacttg ccgcctcgct
     3961 gcaactcgaa atctattagg tatattccat gtggtacttc ttaatattat catcaacaat
     4021 attgattaca tttttttggc tcatcaaatc attcattggt tcaaaggaca gcaatacact
     4081 tttcgcacca cacttttcaa ttgccaactt agccgcagtt atacactccg tataatttcc
     4141 gacagcgttt tctgcaatta tttcttcaag tttattttcg aaattttcat tagggtgcat
     4201 ttcaagaaca taatcactaa taaatgcacg cgtctcttgt ttagctttat tactatcttc
     4261 gttatagtta actaatatca ttaactgatg gtctatctct gataggtcaa cgtcatattt
     4321 atccgcaacg gctttatatc tttcagcata ttcatatcta acatcattag aatcatccca
     4381 cttaaagatg agaggaatac cttttttggc cgcccactca acaatatgat gactggttgc
     4441 tgttacatat ttccgaggtc cgcctggcgt ataagcatgg ggatttacag atattttagg
     4501 gaagctataa aaatcgttat ctggattaca atagcctgtt gttaaagcat cgttaatgat
     4561 ttcataacac tcttcaaata gttgctgttg atattcaacc gggcgattaa aaaaatgcat
     4621 ttcatctttt ttttcgcaat cactaaaccc taaaataaat ctcccttcac ttaactgatc
     4681 caataagcaa gcttcctccg ctatgcggac aggatgatga gttgtaatga tgtgatttaa
     4741 tgaaccaatt ttaattttct ctgttaaacc gagcagaaaa ccagaaacag tcagaggagc
     4801 gccgacaaca ccattatctg aaaaatgatt ttcatacact aaaatctgtt caaaattcaa
     4861 cttatcaaca tactccgtta tttcctgcat gcgaactata ctttgttctt gaacagttgt
     4921 tgaattgatg aagttaagga agaacaatcc aaatttcatt tctttctcct tagctaatat
     4981 aatagcgaac gttgtttttc tttaagaaat ggcatgacat cagactggaa gagcttcatg
     5041 gaagcaataa tttcgtctac tgttccatta gcttcaaatc cacaacaaat atttgatatt
     5101 cctgtagcat caatgtcttt ttgaattatg tcaatacatt cctgcggcgt tcccacggga
     5161 ttgatttcgt aactgtaatc aatacggcga ttagtatctt tatgtccttt taatacaaag
     5221 tcacgccact gccctttatt gaaatcataa cctcttgttt ggtctgaatc atcaaaaata
     5281 gtcgtagcat tcacataaga atcataccaa tgccccagaa atttccggca aatctctttc
     5341 gctttaattg agtcatgatc tacagatgtt atatatgata agcaatggtc gatattatga
     5401 atatcgtgcc catattcttg agccacttca ttataaagct caagttgtgc tttcttttcg
     5461 ttagtattta taatccaact taatatcatc ggtaggccaa attgagcagc ccactcagtc
     5521 gtcgaagctg attcagccac cacataaacc ggtgcgccac ctctgctata cgccgcgggg
     5581 tttactttta ccttatggaa cttgatatgt tcattatcag cttccatata tccctctgtc
     5641 atgccattct ttatcagccc gtaccagcat tccgctaagg cgcgactgtt attcatatct
     5701 gtgccgaata cgcgaaagtc cttgttgtaa agccctcggc aaataccaaa ccgaaatcgt
     5761 ccttttgaca tttgatccaa taaattcaca tcttcaagtt ggcgtactgg atgggctgtg
     5821 ggaagaacaa tagcggcagt tcctacattc aattttttag tcgcgccaag taaatatgca
     5881 gcagcgacat aagggttacc aagcaaacca aactccgtga aatgatgctc cagtaaccat
     5941 acggtatcaa aaccacactc ctcagagatg cgacctaatt taaccaaacg tttcattacc
     6001 tctgtttgag aaaattgggg aggttggtat gtaagcaaaa agtttccaaa tttcatagag
     6061 agtccttata ttgctatttg agtgatagaa tatctcaata gattttaaga cagagaaatt
     6121 gcttgatttt caatctcaat tctcattcgg cgttcattga ctgtcgcaat agttaaatgt
     6181 tcaaatgacg gttcagtaat atcaacatca atatccagat gatcattatc catcgcgata
     6241 gcggctttcg taaccgattg ataaaaattg cgcaggacca ctaaattttc actcaagtca
     6301 tgcgaacttc ctaacaaaga atatatcttg catcgattac tacgaatatt tgataacaat
     6361 gtgataactt catcttgctt gacccaatta tcgttatttg cagtaaaagc aataaacggt
     6421 atatcaagat acatcatgtt attaattgta gaagctaaat cttcccaacc aaaatcaaga
     6481 caatctctcg caaagacttc agcacccaat ttatggcctt caaaatctag attattcggc
     6541 aattcattaa tgggtagact gagataatca aaccctaaag ctctttcaag agaatatctt
     6601 aagttaacaa caccgactgc ggtgattaaa aacgaagcat tgatttcaga taggcttgca
     6661 taagctatcc gcgcagataa gcttgaagcc aacataccga agttatttat ttttcgtgta
     6721 gttaaccaat caaccactgc taacaagctc tgctttccta tagacattgt aaattcatca
     6781 attgtccctg aactcaatcc aacgtggtga agcgaatcat agcggatcac atgaaatcca
     6841 ttccgcgata aatattccgc cagaccagca aaatgatcca tcctgcgggc aaaaccagac
     6901 gcaataataa tggcattctt tctctttggg ctgttttctt ctggcagcgt ttcccaaaca
     6961 tgaatttttt tatttccttc aacacaaata acgtggtcga tggttttata ttttgattca
     7021 ttttccatac tttttaccta ttatgggaca aatacaagga acttatcttc ttccaggaat
     7081 cgagtctgtt ctatttcaac cgcaacatcc ttagccgtat agttagatgg cctttcatga
     7141 gaaatatatg tcactaatcg ttgcaacggt ctcattccgt catgagatcc accaactcga
     7201 aatatgttat tcattcctgc ttctacaatc ctttccgcac cttttaatgc taacgcatct
     7261 cgatatttaa atgatgactc ccaaggaaaa atagatatgg tttgcgtctt atttttttga
     7321 acataaggca atatttgctc aatattatcg acgtgatgaa ggtacacaca tctgccaagt
     7381 ggttgattaa attccacacc tgcatttgac tcaataatca tccaacgttg atgaatatcc
     7441 acctctactt ttaatccagc aaacaagctt tctttttgaa ctaaagaata ggccgccttt
     7501 tcatcaaaat cttttttggc attcggtaat atatgcgcat atagattaag tttttctatc
     7561 aacgctaact taaattcctc ataatgattt cccatgtaat atatgttttg ggcagaaaaa
     7621 caagctcgct gatcgtaaaa acaaacatca tgagccgcac ctgtcgctgc ggacgtcaaa
     7681 tcaacaggat tatcgataat gcaaagactc tttttagaac caaatttaat cacatcagca
     7741 taagatggcg catgctctac cgcccaatta atcgcatctg gccctcccca agcgacaata
     7801 acatccgcat gttgcataat ttcttttgcg agtgatgtat caccttggtg gggccaatat
     7861 ataacagata aagagcgcgt tatcggatga ttagggtcta catcaataaa acttaacgct
     7921 aatgcattag cggtaaaagg atcggttgac gatgttttta taatacactg attcttagtt
     7981 aaaattgcgc gtaatataga catgatccca gataatggaa cattacctgc caacagatgt
     8041 acagatttac ctttcggaaa agcccgaaca taactttcat cctgaggtag ccattcatcc
     8101 atgatatggc gagaaccaag ttcattttct acaacatcat aaaggccgcc tttagaacat
     8161 aaaatcatag atatccaatt ggcctctagc ttagccattt cttctgaata tcccatatat
     8221 ttttttaagt cacgaatgta tgtcctgcgt cttgagtatt cttcattttt ccatctttgc
     8281 cctaccgtat agagaaaatt gacaatgtta tgcaaccgta attcgttatt tccattacaa
     8341 tcaataatgt tttttacatg agagtcattc aatattggca ggtaaacact attatcacca
     8401 aaattaatgg attgcactaa atcatcacct tcgggaaaga tttcaacctg gccgttaata
     8461 atgaatgaaa tttttttagt catacgtatc ctccaagcct gaattcgttt aaacggatcc
     8521 cagcctccct cgcactttcg aaaacgtctt tgcggggcaa ctcgaccatc gtcattctcc
     8581 ttcaccgttg gcctcctctg cgagaggccc tgttcgatgc gaaggaaaat gcaccctgac
     8641 attcattttg ataatctgct taaatcggga aggatcatca actctcagcg gataatccga
     8701 gggcaatatg gatcgactaa ccagcctcat ggtgtttggc cgcgtggtgg aatgcggcgg
     8761 tttttccgcc gccgcgcgcc ggctcaacat gtccgtcacc atggtcggca atcacgtgca
     8821 atcgctggag gaccggctcg gcgtgcggct gcttaaccgc acgacgcgca aggtcagcct
     8881 gacggagacc ggcaaatatt attatgagcg ctcgtcgcag atcctggccg aactcgatga
     8941 ggcggaccgg acggccggcg cgctgagcac gacgccgcgg ggcacgctga aggtctatac
     9001 cagcagtgcc atcgtgcgtt tcctgctgcc cgtcgtcagc gaatttatgg agctttatcc
     9061 gtcgatctca ctcgatttca gcgtcggcga acggatggtc gacatgatcg aggatggcta
     9121 cgacctggtg atgcgcaccg taccgccgcc ggattcatca ctcgtcgccc gcaagctgac
     9181 accctggcgc catatgctcg tctgctcgcc ggcctatttc cagagccatc cgatgccgaa
     9241 gacgccggcc gaggtcgccg atcacaattg cctgcaatat gcctattacc cctatggcga
     9301 cgaatggcgc ttcgaggatg gcgaggggaa caaggagagc gtcaagatca gcggcaacgt
     9361 cgtctccaac agcgccgaga tgctgcgttt cctgacgctg accgggcggg gcatcttcct
     9421 ggcgccgagt ttcgtggtgt tcgacgacat cgccgaaggg cggctggtga agatcatgcc
     9481 ggactaccgg ccggtggaat tcaacatcaa cgccgtctac cccaaccgca gccatctgcc
     9541 gacgaaggtg cggctgttca tcgatctctt ggcggagaga tttgctgagc atcggaagtg
     9601 gatgacgtga ggggtaattt tacattggag gtcacccttc ggtaccccca ccgcggactc
     9661 tgactacaac cgtgcgcaag gcgcattagg gaggatgtat gtatgtaatc gcctgcggca
     9721 tcgttgccgg cttggcggct gcggtggccc tgttgggctt cacgccgatg atggaggcgc
     9781 ttgccgccgg cgaacgccgc aaggcactcg cgcaatggac gcggacgatg ttcctggtgc
     9841 tgctgcctgt cgtgctgatg tgcgcgccca tcgggtccag catttacgac gccgtgcaag
     9901 cggacgctgg caagcccatc gctttccaca acggccggat cacggtcgtc atggccctgg
     9961 tcggcagctt ggccgttgtc ctggtcgcgg ctgcgcgtgc ggtggtcaac cgcaagcatg
    10021 ccagcttctg gttcgtcggc tgggtgatgg cgtcggtttt ggccggcggc gtcggcgcga
    10081 tcgccagcgc gaagcaactg gcgttcctcg gcgaacatag cggcatggtg gccttcggct
    10141 tcttccgcga ccaggtgaag gacatgcact gcgatgcgga cgtgatcctg gcccggtggg
    10201 atgaaaaggc gaactcgccg gtggtctacc gctgcccgaa ggcgtacctg ctcaacaggt
    10261 tcgcatccgc gcccttcgtg ccctggccgg actacaccga gggggaaagc gaggatctag
    10321 gtagggcgct cgcagcggcc ctgcgggacg cgaaaaggtg agaaaagccg ggcactgccc
    10381 ggctttattt ttgctgctgc gcgttccagg ccgcccacac tcgtttgacc tggctcgggc
    10441 tgcatccgac cagcttggcc gtcttggcaa tgctcgatcc gccggagcga agcgtgatga
    10501 tgcggtcgtg catgccggcg tcacgtttgc ggccggtgta gcggccggcg gccttcgcca
    10561 actggacacc ctgacgttga cgctcgcgcc gatcctcgta gtcgtcgcgg gccatctgca
    10621 aggcgagctt caaaagcatg tcctggacgg attccagaac gattttcgcc actccgttcg
    10681 cctcggcggc cagctccgac aggtccacca cgccaggcac ggccagcttg gcccctttgg
    10741 cccggatcga cgcaaccagg cgctcggcct cggccaacgg caagcggctg atgcggtcga
    10801 tcttctccgc aacgacgact tcaccaggtt gcaggtccgc gatcatgcgc agcagctcgg
    10861 gccggtcggc gcgtgcgccg gacgccttct cgcggtagat gccggcgacg tagtacccgg
    10921 cggcccgcgt ggccgctaca aggctctcct ggcgttcaag attctgctcg tccgtactgg
    10981 cgcgcaggta gatgcgggcg accttcaacc ttcgtccctc cggttgttgc tctcgcgtcg
    11041 ccatttccac ggctcgacgg cgtgcggatc ggaccagagg ccgacgcgct tgcctcgcgc
    11101 ctcctgttcg agccgcagca tttcagggtc ggccgcgcgg ccgtggaagc gataggccca
    11161 cgccatgccc tggtgaacca tcgcggcgtt gacgttgcgc ggctgcggcg gccggctggc
    11221 cagctccatg ttgacccaca cggtgcccag cgtgcggccg taacggtcgg tgtccttctc
    11281 gtcgaccagg acgtgccggc ggaacaccat gccggccagc gcctggcgcg cacgttcgcc
    11341 gaaggcttgc cgcttttccg gcgcgtcaat gtccaccagg cgcacgcgca ccggctgctt
    11401 gtctaccagc acgtcgatgg tgtcgccgtc gatgatgcgc acgacctcgc cgcgcagctc
    11461 ggcccatgcc ggcgaggcaa cgaccaggac ggccagcgcg gcagcggcgc gcagcatggc
    11521 gtagcttcgg cgcttcatgc gtggccccat tgctgatgat cggggtacgc caggtgcagc
    11581 actgcatcga aattggcctt gcagtagccg tccagcgcca cccgcgagcc gaacgccggc
    11641 gaaaggtact cgaccaggcc gggccggtcg cggacctcgc gccccaggac gtggatgcgc
    11701 cggccgcgtg tgccgtcggg tccaggcacg aaggccagcg cctcgatgtt gaagtcgatg
    11761 gatagaagtt gtcggtagtg cttggccgcc ctcatcgcgt cccccttggt caaattgggt
    11821 atacccattt gggcctagtc tagccggcat ggcgcattac agcaatacgc aatttaaatg
    11881 cgcctagcgc attttcccga ccttaatgcg cctcgcgctg tagcctcacg cccacatatg
    11941 tgctaatgtg gttacgtgta ttttatggag gttatccaat gagccgcctg acaatcgaca
    12001 tgacggacca gcagcaccag agcctgaaag ccctggccgc cttgcagggc aagaccatta
    12061 agcaatacgc cctcgaacgt ctgttccccg gtgacgctga tgccgatcag gcatggcagg
    12121 aactgaaaac catgctgggg aaccgcatca acgatgggct tgccggcaag gtgtccacca
    12181 agagcgtcgg cgaaattctt gatgaagaac tcagcgggga tcgcgcttga cggcctacat
    12241 cctcacggct gaggccgaag ccgatctacg cggcatcatc cgctacacgc gccgggagtg
    12301 gggcgcggcg caggtgcgcc gctatatcgc taagctggaa cagggcatag ccaggcttgc
    12361 cgccggcgaa ggcccgttta aggacatgag cgaactcttt cccgcgctgc ggatggcccg
    12421 ctgcgaacac cactacgttt tttgcctgcc gcgtgcgggc gaacccgcgt tggtcgtggc
    12481 gatcctgcat gagcgcatgg acctcatgac gcgacttgcc gacaggctca agggctgatt
    12541 tcagccgcta aaaatcgcgc cactcacaac gtcctgatgg cgtacttacc caaagaacag
    12601 ctaggagaat catttatgct cagcacactt ccacaagctc atgcaacttt cttgaaccgc
    12661 atccgcgatg cggtcgcttc cgatgttcgc ttccgcgctc ttctgatcgg cggctcttac
    12721 gttcacggag gactcgatga gcactccgat ttggatttcg acatcgttgt tgaggacaac
    12781 tgctacgcag atgtcttgtc tacacgcaag gattttgccg aggcactgcc cggcttcctc
    12841 aacgcgttca ccggcgaaca tgtaggagaa ccgcgccttc tgatctgcct atatggtccg
    12901 ccactgctac acatcggggg atcagcttgg cgtaatcatg gtcatagctg tttcctgtgt
    12961 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
    13021 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
    13081 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
    13141 gcggtttgcg tattggaatt aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
    13201 tataggttaa tgtcatgata ataatggttt cttagagctt atcggccagc ctcgcagagc
    13261 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
    13321 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
    13381 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
    13441 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagatcc gtcgatcgac
    13501 ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
    13561 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
    13621 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
    13681 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
    13741 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
    13801 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
    13861 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
    13921 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    13981 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    14041 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    14101 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    14161 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    14221 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    14281 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    14341 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    14401 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    14461 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    14521 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    14581 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    14641 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgggggat caggaccgct
    14701 gccggagcgc aacccactca ctacagcaga gccatgtaga caacatcccc tccccctttc
    14761 caccgcgtca gacgcccgta gcagcccgct acgggctttt tcatgccctg ccctagcgtc
    14821 caagcctcac ggccgcgctc ggcctctctg gcggccttct ggcgctcctg ctgcggcgtc
    14881 cgctcgtggg ccgtggcgcg ggtccgcgcg ccggcctcgt gcgcctggcg ctcgcgggcg
    14941 aggtccaggg cggccgtctt cacgttctgc cttgcgcaga tgagatagat ccgtcaattc
    15001 cggcctatcg tttccacgat cagcgatcgg ctcgttgccc tgcgccgctc caaagcccgc
    15061 gacgcagcgc cggcaggcag agcaagtaga gggcagcgcc tgcaatccat gcccacccgt
    15121 tccacgttgt tatagaagcc gcatagatcg ccgtgaagag gaggggtccg acgatcgagg
    15181 tcaggctggt gagcgccgcc agtgagcctt gcagctgccc ctgacgttcc tcatccacct
    15241 gcctggacaa cattgcttgc agcgccggca ttccgatgcc acccgaagca agcaggacca
    15301 tgatcgggaa cgccatccat ccccgtgtcg cgaaggcaag caggatgtag cctgtgccgt
    15361 cggcaatcat tccgagcatg agtgcccgcc tttcgccgag ccgggcggct acagggccgg
    15421 tgatcattgc ctgggcgagt gaatgcagaa tgccaaatgc ggcaagcgaa atgccgatcg
    15481 tggtcgcgtc ccagtgaaag cgatcctcgc cgaaaatgac ccaaagcgcg gccggcacct
    15541 gtccgacaag ttgcatgatg aagaagaccg ccatcagggc ggcgacgacg gtcatgcccc
    15601 gggcccaccg gaacgaagcg agcgggttga gagcctcccg gcgtaacggc cggcgttcgc
    15661 ctttgtgcga ctccggcaaa aggaaacagc ccgtcaggaa attgaggccg ttcaaggctg
    15721 ccgcggcgaa gaacggagcg tggggggaga aaccgcccat cagcccaccg agcacaggtc
    15781 ccgcgaccat cccgaacccg aaacaggcgc tcatgaagcc gaagtgccgc gcgcgctcat
    15841 cgccatcagt gatatcggca atataagcgc cggctaccgc cccagtcgcc ccggtgatgc
    15901 cggccacgat ccgcccgata tagagaaccc aaaggaaagg cgctgtcgcc atgatggcgt
    15961 agtcgacagt ggcgccggcc agcgagacga gcaagattgg ccgccgcccg aaacgatccg
    16021 acagcgcgcc cagcacaggt gcgcaggcaa attgcaccaa cgcatacagc gccagcagaa
    16081 tgccatagtg ggcggtgacg tcgttcgagt gaaccagatc gcgcaggagg cccggcagca
    16141 ccggcataat caggccgatg ccgacagcgt cgagcgcgac agtgctcaga attacgatca
    16201 ggggtatgtt gggtttcacg tctggcctcc ggaccagcct ccgctggtcc gattgaacgc
    16261 gcggattctt tatcactgat aagttggtgg acatattatg tttatcagtg ataaagtgtc
    16321 aagcatgaca aagttgcagc cgaatacagt gatccgtgcc gccctggacc tgttgaacga
    16381 ggtcggcgta gacggtctga cgacacgcaa actggcggaa cggttggggg ttcagcagcc
    16441 ggcgctttac tggcacttca ggaacaagcg ggcgctgctc gacgcactgg ccgaagccat
    16501 gctggcggag aatcatacgc attcggtgcc gagagccgac gacgactggc gctcatttct
    16561 gatcgggaat gcccgcagct tcaggcaggc gctgctcgcc taccgcgatg gcgcgcgcat
    16621 ccatgccggc acgcgaccgg gcgcaccgca gatggaaacg gccgacgcgc agcttcgctt
    16681 cctctgcgag gcgggttttt cggccgggga cgccgtcaat gcgctgatga caatcagcta
    16741 cttcactgtt ggggccgtgc ttgaggagca ggccggcgac agcgatgccg gcgagcgcgg
    16801 cggcaccgtt gaacaggctc cgctctcgcc gctgttgcgg gccgcgatag acgccttcga
    16861 cgaagccggt ccggacgcag cgttcgagca gggactcgcg gtgattgtcg atggattggc
    16921 gaaaaggagg ctcgttgtca ggaacgttga aggaccgaga aagggtgacg attgatcagg
    16981 accgctgccg gagcgcaacc cactcactac agcagagcca tgtagacaac atcccctccc
    17041 cctttccacc gcgtcagacg cccgtagcag cccgctacgg gctttttcat gccctgccct
    17101 agcgtccaag cctcacggcc gcgctcggcc tctctggcgg ccttctggcg ctcctgctgc
    17161 ggcgtccgct cgtgggccgt ggcgcgggtc cgcgcgccgg cctcgtgcgc ctggcgctcg
    17221 cgggcgaggt ccagggcggc cgtcttcacg ttctgccttg cgcagatgag atagatcccg
    17281 gaattgacgg atctagcgtg gactcaaggc tctcgcgaat ggctcgcgtt ggaaactttc
    17341 attgacactt gaggggca
//
