LOCUS       sequence-171065-        7947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBAc transposon with transformation marker for Tribolium 
            transgenesis; Rescues vermilion white eyed mutant to black eyes; 
            (transformation plasmid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7947)
  TITLE     Bucher Lab plasmids
REFERENCE   2  (bases 1 to 7947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBac[3xP3 g Tc' v]
FEATURES             Location/Qualifiers
     source          1..7947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1812..1933
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1849..1868)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1903..1922
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   3366..3400
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(3581..3597)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3581..3597)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3594..3616)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3605..3621
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3629..3635,3636..3653,3654..3659))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3674..3695
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3812..3829)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3983..4571)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4063..4082)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4742..5533,5534..5602))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5365..5384
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5603..5707)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5775..5793
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5831..5853)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5953..5972
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6166..6188
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6180..6197
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6181..6197
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   6901..6963
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 gttaattcga gctcgcccgg ggatctaatt caattagaga ctaattcaat tagagctaat
       61 tcaattagga tccaagctta tcgatttcga accctcgacc gccggagtat aaatagaggc
      121 gcttcgtcta cggagcgaca attcaattca aacaagcaaa gtgaacacgt cgctaagcga
      181 aagctaagca aataaacaag cgcagctgaa caagctaaac aatcggggta ccgctagagt
      241 cgacggtacc gcggtcgtaa tgagttgccc actgagaccc tcgtaagtat gatttaattt
      301 aattattcca tttacaatta atcgtaaatt aagtgaagcc caagaaggcg accaactgag
      361 cgaagaatgt gggatgcttt acggtgaata cttgatgttg gacaagattt tagaagccca
      421 aagactactc agcgagcaaa ataaccaacc cgtgcacgac gagcacttat ttattgtgac
      481 ccatcaaggt aaaaaaccca cttaaccgta attaactata acaagtaatt ccagcgtatg
      541 agttatggtt caaacaaata atctacgagc tggactcgat ccgcaacatt ttcagcgacg
      601 ttttagaaga gtcgcaaact ttggaaatcc tcaaacgcct caatcgtgtt gttttgatcc
      661 tcaaggttca caatttcgca acaaattgcg gtcacgagat aaaactgtcg caattgtagg
      721 ttttggtgga ccaagtaatg attctggaaa cgatgacacc gctcgatttc atggacttcc
      781 ggtgttatct gcggcccgct tccgggttcc agagcctgca gttcaggctc ctggaaaata
      841 agctcggggt gcgccaggaa aaccgcgtca aatacaacca gaattactcg aaagtgttcg
      901 gaaacgacga aaaagcgctc gagcaaatcg ccaagtcgga gaaagagccg tctttgaccg
      961 atttggtcca gcggtggctg gagcgaacgc cggggctgga gctcgagggg tttaatttct
     1021 gggggaagta ccaaaaagcg gtcgagaagc ttttaaccga gcaaaaagaa ctggcggaga
     1081 aagaggaggc ggagacttta aagcgttaca aattgaacga tttggagaag agacgcgagg
     1141 tttacgagtc gatttttaaa gttgaggtcc atgaggctct aatgtcgcgc ggcgagaggc
     1201 gcttctcaca caaagccctg caaggggcta tcatgatcac cttttacagg gacgaaccca
     1261 ggttcagtca gccccaccaa attctaacac ttttgatgga catagactcg ctgattacga
     1321 aatggcggtg ttagtaaatc cgtacgtcaa ttgtttttgt ttaaatttta taatttcaga
     1381 taatcatgtt ttgatggtgc aaagaatgat tggttcttcg cagttaggaa cagggggctc
     1441 ttcggggtat cagtatttaa ggtcaacgtt aaggtgaaat tgatttcaac acggtttttg
     1501 caatgtttga ttttgttttt cagcgatcgg tataaagttt tcgtggacct tttcaattta
     1561 tcgacgtttt tgataccacg gtcctacatc ccccccttgt ctacgtctat gagaagtcat
     1621 ttgtgcaatt gggggtcggc gaatagcacc aataacgtgt cgaatggaaa caattaatta
     1681 tttcgttaag gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt
     1741 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     1801 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     1861 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     1921 tcaatgtatc ttaaagctta tcgatacgcg tacggcgcgc ctaggccggc cgatctcgaa
     1981 cttatacggt tcttgtagta agttttttgc caaagggatt gaggtgaacc aattgtcaca
     2041 cgtaatatta cgacaactac cgtgcacagg ctttgataac tccttcacgt agtattcacc
     2101 gagtggtact ccgttggtct gtgttcctct tcccaaataa ggcattccat ttatcatata
     2161 cttcgtacca ctgtcacaca tcatgaggat ttttattcca tacttacttg gcttgtttgg
     2221 gatatacatc ctaaacggac accgtcctct aaaaccaagt aactgttcat ctatggtcaa
     2281 atgagcccct ggagtgtaat tttgtatgca ctgatggata aagagatccc atatttttct
     2341 aacaggagta aatacatcgt tttctcgaag tgtgggccgt atacttttgt catccattct
     2401 aagacatcgt atcaaaaaat caaaacgatc acgactcatt acagagacgt acaccattga
     2461 caaagatcga tcaaagaggt catctgtgga catgtggtta tcttttctca ctgctgtcat
     2521 taccagaata ccaaagaaag catagatttc atcttcattc gtgtcacgaa atgtagcacc
     2581 tgtcatagat tcccgacgtt tcaatgatat ctcagcattt gtccatttta caatttccga
     2641 aattatctca tcagtaaaaa atagtttgaa gcataaaagt gggtcatata tattgcggca
     2701 catacgcgtc ggacctcttt gagatctgac aatgttcagt gcagagactc ggctacgcct
     2761 cgtggacttt gaagttgacc aacaatgttt attcttacct ctaatagtcc tctgtggcaa
     2821 ggtcaagatt ctgttagaag ccaatgaaga acctggttgt tcaataacat tttgttcgtc
     2881 taatatttca ctaccgcttg acgttggctg cacttcatgt acctcatcta taaacgcttc
     2941 ttctgtatcg ctctggacgt catcttcact tacgtgatct gatatttcac tgtcagaatc
     3001 ctcaccaaca agctcgtcat cgctttgcag aagagcagag aggatatgct catcgtctaa
     3061 agaactaccc attttattat atattagtca cgatatctat aacaagaaaa tatatatata
     3121 ataagttatc acgtaagtag aacatgaaat aacaatataa ttatcgtatg agttaaatct
     3181 taaaagtcac gtaaaagata atcatgcgtc attttgactc acgcggtcgt tatagttcaa
     3241 aatcagtgac acttaccgca ttgacaagca cgcctcacgg gagctccaag cggcgactga
     3301 gatgtcctaa atgcacagcg acggattcgc gctatttaga aagagagagc aatatttcaa
     3361 gaatgcatgc gtcaatttta cgcagactat ctttctaggg ttaaaaaaga tttgcgcttt
     3421 actcgaccta aactttaaac acgtcataga atcttcgttt gacaaaaacc acattgtggc
     3481 caagctgtgt gacgcgacgc gcgctaaaga atggcaaacc aagtcgcgcg agcgtcgact
     3541 ctagaggatc cccgggtacc gagctcgaat tcgtaatcat gtcatagctg tttcctgtgt
     3601 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     3661 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     3721 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     3781 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3841 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3901 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3961 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4021 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4081 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4141 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     4201 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4261 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4321 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4381 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     4441 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4501 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4561 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4621 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4681 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     4741 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     4801 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     4861 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     4921 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     4981 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5041 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     5101 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     5161 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     5221 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     5281 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     5341 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     5401 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     5461 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     5521 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     5581 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     5641 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     5701 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     5761 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     5821 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     5881 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     5941 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     6001 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
     6061 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     6121 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     6181 gtaaaacgac ggccagtgcc aagctttgtt taaaaatata acaaaattgt gatcccacaa
     6241 aatgaagtgg ggcaaaatca aataattaat agtgtccgta aacttgttgg tcttcaactt
     6301 tttgaggaac acgttggacg gcaaatccgt gactataaca caagttgatt taataatttt
     6361 agccaacacg tcgggctgcg tgttttttgc cgacgcgtct gtgtacacgt tgattaactg
     6421 gtcgattaaa ctgttgaaat aatttaattt ttggttcttc tttaaatctg tgatgaaatt
     6481 ttttaaaata actttaaatt cttcattggt aaaaaatgcc acgttttgca acttgtgagg
     6541 gtctaatatg aggtcaaact cagtaggagt tttatccaaa aaagaaaaca tgattacgtc
     6601 tgtacacgaa cgcgtattaa cgcagagtgc aaagtataag agggttaaaa aatatatttt
     6661 acgcaccata tacgcatcgg gttgatatcg ttaatatgga tcaatttgaa cagttgatta
     6721 acgtgtctct gctcaagtct ttgatcaaaa cgcaaatcga cgaaaatgtg tcggacaata
     6781 tcaagtcgat gagcgaaaaa ctaaaaaggc tagaatacga caatctcaca gacagcgttg
     6841 agatatacgg tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa
     6901 ccctagaaag ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc
     6961 atgtgtttta tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt
     7021 tattatattt acacttacat actaataata aattcaacaa acaatttatt tatgtttatt
     7081 tatttattaa aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa
     7141 cattctctct tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta
     7201 taaaataagt aattagctta acttatacat aatagaaaca aattatactt attagtcagt
     7261 cagaaacaac tttggcacat atcaatatta tgctctcgac aaataacttt tttgcatttt
     7321 ttgcacgatg catttgcctt tcgccttatt ttagaggggc agtaagtaca gtaagtacgt
     7381 tttttcatta ctggctcttc agtactgtca tctgatgtac caggcacttc atttggcaaa
     7441 atattagaga tattatcgcg caaatatctc ttcaaagtag gagcttctaa acgcttacgc
     7501 ataaacgatg acgtcaggct catgtaaagg tttctcataa attttttgcg actttgaacc
     7561 ttttctccct tgctactgac attatggctg tatataataa aagaatttat gcaggcaatg
     7621 tttatcattc cgtacaataa tgccataggc cacctattcg tcttcctact gcaggtcatc
     7681 acagaacaca tttggtctag cgtgtccact ccgcctttag tttgattata atacataacc
     7741 atttgcggtt taccggtact ttcgttgata gaagcatcct catcacaaga tgataataag
     7801 tataccatct tagctggctt cggtttatat gagacgagag taaggggtcc gtcaaaacaa
     7861 aacatcgatg ttcccactgg cctggagcga ctgtttttca gtacttccgg tatctcgcgt
     7921 ttgtttgatc gcacggttcc cacaatg
//
