LOCUS       sequence-171071-        7059 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Gq-coupled hM3D DREADD fused with mCherry under the control of human
            synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7059)
  AUTHORS   Bryan Roth
  TITLE     Roth lab DREADDs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-hSyn-hM3D(Gq)-mCherry
FEATURES             Location/Qualifiers
     source          1..7059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..23
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        147..251
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(252..320,321..1112)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(470..489)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1283..1871
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1772..1791
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   1933..2062
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        2107..2554
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      4377..4386
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4383..5093
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4528..4546)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4796..4815
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5118..5706
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5171..5191)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5589..5600)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5738..6214
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5937..5956)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   6254..6394
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6254..6383
                     /label=AAV2 ITR
     rep_origin      6469..6924
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6556..6575)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6766..6787
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6941..6960)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
       61 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      121 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      181 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      241 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      301 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      361 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      421 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      481 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      541 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      601 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      661 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      721 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      781 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      841 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      901 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      961 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1021 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1081 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1141 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1201 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1261 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1321 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1381 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1441 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1501 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1561 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1621 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1681 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1741 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1801 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1861 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1921 ttgctcacat gtcctgcagg cagctgcgcg ctcgctcgct cactgaggcc gcccgggcgt
     1981 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc
     2041 aactccatca ctaggggttc ctgcggccgc acgcgtgtgt ctagactgca gagggccctg
     2101 cgtatgagtg caagtgggtt ttaggaccag gatgaggcgg ggtgggggtg cctacctgac
     2161 gaccgacccc gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct
     2221 atcagagagg gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc
     2281 accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga
     2341 aggcgcgctg acgtcactcg ccggtccccc gcaaactccc cttcccggcc accttggtcg
     2401 cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca
     2461 cgggcgcgac catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg
     2521 cagcggagga gtcgtgtcgt gcctgagagc gcagtcgaga aggtaccgga tcctctagag
     2581 tcgacgccac catgaccttg cacaataaca gtacaacctc gcctttgttt ccaaacatca
     2641 gctcctcctg gatacacagc ccctccgatg cagggctgcc cccgggaacc gtcactcatt
     2701 tcggcagcta caatgtttct cgagcagctg gcaatttctc ctctccagac ggtaccaccg
     2761 atgaccctct gggaggtcat accgtctggc aagtggtctt catcgctttc ttaacgggca
     2821 tcctggcctt ggtgaccatc atcggcaaca tcctggtaat tgtgtcattt aaggtcaaca
     2881 agcagctgaa gacggtcaac aactacttcc tcttaagcct ggcctgtgcc gatctgatta
     2941 tcggggtcat ttcaatgaat ctgtttacga cctacatcat catgaatcga tgggccttag
     3001 ggaacttggc ctgtgacctc tggcttgcca ttgactgcgt agccagcaat gcctctgtta
     3061 tgaatcttct ggtcatcagc tttgacagat acttttccat cacgaggccg ctcacgtacc
     3121 gagccaaacg aacaacaaag agagccggtg tgatgatcgg tctggcttgg gtcatctcct
     3181 ttgtcctttg ggctcctgcc atcttgttct ggcaatactt tgttggaaag agaactgtgc
     3241 ctccgggaga gtgcttcatt cagttcctca gtgagcccac cattactttt ggcacagcca
     3301 tcgctggttt ttatatgcct gtcaccatta tgactatttt atactggagg atctataagg
     3361 aaactgaaaa gcgtaccaaa gagcttgctg gcctgcaagc ctctgggaca gaggcagaga
     3421 cagaaaactt tgtccacccc acgggcagtt ctcgaagctg cagcagttac gaacttcaac
     3481 agcaaagcat gaaacgctcc aacaggagga agtatggccg ctgccacttc tggttcacaa
     3541 ccaagagctg gaaacccagc tccgagcaga tggaccaaga ccacagcagc agtgacagtt
     3601 ggaacaacaa tgatgctgct gcctccctgg agaactccgc ctcctccgac gaggaggaca
     3661 ttggctccga gacgagagcc atctactcca tcgtgctcaa gcttccgggt cacagcacca
     3721 tcctcaactc caccaagtta ccctcatcgg acaacctgca ggtgcctgag gaggagctgg
     3781 ggatggtgga cttggagagg aaagccgaca agctgcaggc ccagaagagc gtggacgatg
     3841 gaggcagttt tccaaaaagc ttctccaagc ttcccatcca gctagagtca gccgtggaca
     3901 cagctaagac ttctgacgtc aactcctcag tgggtaagag cacggccact ctacctctgt
     3961 ccttcaagga agccactctg gccaagaggt ttgctctgaa gaccagaagt cagatcacta
     4021 agcggaaaag gatgtccctg gtcaaggaga agaaagcggc ccagaccctc agtgcgatct
     4081 tgcttgcctt catcatcact tggaccccat acaacatcat ggttctggtg aacacctttt
     4141 gtgacagctg catacccaaa accttttgga atctgggcta ctggctgtgc tacatcaaca
     4201 gcaccgtgaa ccccgtgtgc tatgctctgt gcaacaaaac attcagaacc actttcaaga
     4261 tgctgctgct gtgccagtgt gacaaaaaaa agaggcgcaa gcagcagtac cagcagagac
     4321 agtcggtcat ttttcacaag cgcgcacccg agcaggcctt gaaggatccc ccggtcgcca
     4381 ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     4441 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     4501 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     4561 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     4621 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     4681 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     4741 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     4801 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     4861 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     4921 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     4981 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     5041 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag taagaattcg
     5101 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     5161 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     5221 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     5281 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     5341 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     5401 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     5461 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     5521 cctttccttg gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct
     5581 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5641 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5701 ccccgcatcg ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc
     5761 cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat
     5821 aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag
     5881 gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta
     5941 ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg
     6001 ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac
     6061 caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct
     6121 ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt
     6181 acaggcgtga accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg
     6241 accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
     6301 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
     6361 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt
     6421 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
     6481 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     6541 gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6601 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     6661 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     6721 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     6781 aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc
     6841 cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     6901 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
     6961 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     7021 tgctcccggc atccgcttac agacaagctg tgaccgtct
//
