LOCUS       sequence-171084-        1533 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent biosensor for super-resolution imaging of caspase 
            activity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Mo GC, Ross B, Hertel F, Manna P, Yang X, Greenwald E, Booth C, 
            Plummer AM, Tenner B, Chen Z, Wang Y, Kennedy EJ, Cole PA, Fleming 
            KG, Palmer A, Jimenez R, Xiao J, Dedecker P, Zhang J
  TITLE     Genetically encoded biosensors for visualizing live-cell biochemical
            activity at super-resolution.
  JOURNAL   Nat Methods. 2017 Apr;14(4):427-434. doi: 10.1038/nmeth.4221. Epub 
            2017 Mar 13.
  PUBMED    28288122
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4221"; journalName: "Nat Methods"; date: "2017-04"; 
            volume: "14"; issue: "4"; pages: "427-434"
COMMENT     Sequence Label: Dronpa-DEVD-TagRFPT
FEATURES             Location/Qualifiers
     source          1..1533
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..66
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(70..78,79..93)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             100..771
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKVK
                     EGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNAT
                     NDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALSL
                     EGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPRQ
                     AK"
     CDS             796..1485
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGH"
ORIGIN
        1 atgcggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa
       61 atgggtcggg atctgtacga cgatgacgat aaggatccga tgagtgtgat taaaccagac
      121 atgaagatca agctgcgtat ggaaggcgct gtaaatggac acccgttcgc gattgaagga
      181 gttggccttg ggaagccttt cgagggaaaa cagagtatgg accttaaagt caaagaaggc
      241 ggacctctgc ctttcgccta tgacatcttg acaactgtgt tctgttacgg caacagggta
      301 ttcgccaaat acccagaaaa tatagtagac tatttcaagc agtcgtttcc tgagggctac
      361 tcttgggaac gaagcatgaa ttacgaagac gggggcattt gtaacgcgac aaacgacata
      421 accctggatg gtgactgtta tatctatgaa attcgatttg atggtgtgaa ctttcctgcc
      481 aatggtccag ttatgcagaa gaggactgtg aaatgggagc catccactga gaaattgtat
      541 gtgcgtgatg gagtgctgaa gggtgatgtt aacatggctc tgtcgcttga aggaggtggc
      601 cattaccgat gtgacttcaa aactacttat aaagctaaga aggttgtcca gttgccagac
      661 tatcactttg tggaccacca cattgagatt aaaagccacg acaaagatta cagtaatgtt
      721 aatctgcatg agcatgccga agcgcattct gagctgccga ggcaggccaa gagatctgac
      781 gaggtggacg gtaccatgag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg
      841 gagggcaccg tgaacaacca ccacttcaag tgcacatccg agggcgaagg caagccctac
      901 gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc
      961 gacatcctgg ctaccagctt catgtacggc agcagaacct tcatcaacca cacccagggc
     1021 atccccgatt tctttaagca gtccttccct gagggcttca catgggagag agtcaccaca
     1081 tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc
     1141 atctacaacg tcaagatcag aggggtgaac ttcccatcca acggccctgt gatgcagaag
     1201 aaaacactcg gctgggaggc caacaccgag atgctgtacc ccgctgacgg cggcctggaa
     1261 ggcagaaccg acatggccct gaagctcgtg ggcgggggcc acctgatctg caacttcaag
     1321 accacataca gatccaagaa acccgctaag aacctcaaga tgcccggcgt ctactatgtg
     1381 gaccacagac tggaaagaat caaggaggcc gacaaagaga cctacgtcga gcagcacgag
     1441 gtggctgtgg ccagatactg cgacctccct agcaaactgg ggcacaagga attcaagaag
     1501 aagaagaaga gcaagaccaa gtgcgtgatc atg
//
