LOCUS       sequence-171125-        6033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human UBXD1 with C-terminal GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Papadopoulos C, Kirchner P, Bug M, Grum D, Koerver L, Schulze N, 
            Poehler R, Dressler A, Fengler S, Arhzaouy K, Lux V, Ehrmann M, 
            Weihl CC, Meyer H
  TITLE     VCP/p97 cooperates with YOD1, UBXD1 and PLAA to drive clearance of 
            ruptured lysosomes by autophagy.
  JOURNAL   EMBO J. 2016 Oct 17. pii: e201695148.
  PUBMED    27753622
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2016 Oct 17. pii: e201695148."
COMMENT     Sequence Label: UBXD1-GFP
FEATURES             Location/Qualifiers
     source          1..6033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1333..1342
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1339..1341,1342..1344,1345..2058)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1339..1341,1342..1344,1345..2055)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1384..1405)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1645..1664)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1992..2013
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2181..2302
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2218..2237)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2272..2291
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2309..2764)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2446..2467)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2658..2677
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2791..2895
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2897..3254
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2897..3088
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2920..2940)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3105..3240
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3289..4083
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3289..4083
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3343..3362)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3953..3972
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4271..4290)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4315..4362
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4691..5279
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5180..5199
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5454..5757
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5758..5961
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5912..5932
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atgaagaaat tctttcagga gttcaaggcc gacatcaagt tcaagagcgc gggacccggt
       61 cagaagctca aagagtccgt gggggaaaag gcccacaaag agaagcccaa ccagccagcc
      121 cccaggccgc cccgccaggg acccaccaat gaggcacaga tggcagccgc tgctgcccta
      181 gcccggctgg agcagaagca gtcccgggcc tggggcccca catcgcagga caccatccga
      241 aaccaggtga gaaaggaact tcaagccgaa gccaccgtca gcgggagccc cgaggcccca
      301 gggaccaacg tggtatctga gcccagagag gaaggctctg cccacctggc tgtgcctggc
      361 gtgtacttca cctgtccgct cactggggcc accctgagga aggaccagcg ggacgcctgc
      421 atcaaggagg ccattctctt gcacttctcc accgacccag tggccgcctc catcatgaag
      481 atctacacgt tcaacaaaga ccaggaccgg gtgaagctgg gtgtggacac cattgccaag
      541 tacctggaca acatccacct gcaccccgag gaggagaagt accggaagat caagctgcag
      601 aacaaggtgt ttcaggagcg cattaactgc ctggaaggga cccacgagtt ttttgaggcc
      661 attgggttcc agaaggtgtt gcttcccgcc caggatcagg aggaccccga ggagttctac
      721 gtgctgagcg agaccacctt ggcccagccc cagagcctgg agaggcacaa ggaacagctg
      781 ctggctgcgg agcccgtgcg cgccaagctg gacaggcagc gccgcgtctt ccagccctcg
      841 cccctggcct cgcagttcga actgcctggg gacttcttca acctcacagc agaggagatc
      901 aagcgggagc agaggctcag gtccgaggcg gtggagcggc tgagcgtgct gcggaccaag
      961 gccatgcggg agaaggagga gcagcggggg ctgcgcaagt acaactacac gctgctgcgc
     1021 gtgcgcctcc ccgatggctg cctcctgcag ggcactttct acgctcggga gcggctgggg
     1081 gcggtgtacg ggttcgtccg ggaggccctg cagagcgact ggctgccttt tgagctgctg
     1141 gcctcgggag ggcagaagct gtccgaggac gagaacctgg ccttgaacga gtgcgggctg
     1201 gtgccctctg ccctcctgac cttctcgtgg gacatggctg tgctggagga catcaaggcc
     1261 gcgggggccg agccggactc catcctgaaa cccgagctcc tgtcagccat cgagaagctc
     1321 ttgggatcca tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     1381 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     1441 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1501 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     1561 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     1621 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1681 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1741 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     1801 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     1861 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1921 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     1981 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     2041 gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg
     2101 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     2161 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     2221 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     2281 ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc
     2341 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     2401 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     2461 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     2521 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     2581 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     2641 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     2701 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     2761 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     2821 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     2881 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     2941 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3001 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3061 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     3121 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3181 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3241 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     3301 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     3361 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     3421 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     3481 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     3541 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     3601 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     3661 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     3721 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     3781 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     3841 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     3901 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     3961 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     4021 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     4081 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     4141 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     4201 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
     4261 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     4321 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     4381 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     4441 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     4501 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt
     4561 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4621 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4681 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4741 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4801 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     4861 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4921 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4981 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5041 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5101 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5161 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5221 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5281 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5341 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat
     5401 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     5461 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     5521 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     5581 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     5641 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     5701 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     5761 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     5821 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     5881 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     5941 gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggact
     6001 cagatctcga gctcaagctt cgaattcgcc gcc
//
