LOCUS       sequence-171159-        9162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to express sgRNAs. The cloning site is BsmBI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9162)
  AUTHORS   Fulco CP, Munschauer M, Anyoha R, Munson G, Grossman SR, Perez EM, 
            Kane M, Cleary B, Lander ES, Engreitz JM
  TITLE     Systematic mapping of functional enhancer-promoter connections with 
            CRISPR interference.
  JOURNAL   Science. 2016 Sep 29. pii: aag2445.
  PUBMED    27708057
REFERENCE   2  (bases 1 to 9162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Sep 29. pii: aag2445."
COMMENT     Sequence Label: sgOpti
FEATURES             Location/Qualifiers
     source          1..9162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..69,70..861)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(219..238)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1032..1620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1521..1540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1774..1791
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1908..1929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1944..1949,1950..1967,1968..1974)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1982..1998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1987..2009
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2006..2022
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2006..2022
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2041..2061
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2043..2061
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2089..2315
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2316..2496
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2543..2668
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3161..3394
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3579..3623
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        3801..4049
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3801..3821
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3972..3991
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    6065..6182
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6251..6462
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             6488..7087
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6488..6507)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6984..7004
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7103..7691
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7156..7176)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7574..7585)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7763..7996
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8074..8195
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8111..8130)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8165..8184
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8235..8370
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8390..8409)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8391..8409)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8419..8436)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8419..8435)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8428..8450)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8577..9032
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8664..8683)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8874..8895
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9058..9162
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
       61 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      241 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      361 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
      421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      541 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      601 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
      661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
      721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      841 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
      901 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
      961 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1141 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1621 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1681 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     1741 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     1801 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     1861 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     1921 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     1981 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     2041 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     2101 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     2161 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     2221 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     2281 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     2341 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     2401 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     2461 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     2521 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     2581 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     2641 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     2701 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     2761 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     2821 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     2881 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     2941 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     3001 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     3061 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     3121 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     3181 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     3241 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     3301 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     3361 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     3421 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     3481 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     3541 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     3601 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     3661 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     3721 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     3781 caaccccgag gggacccaga gagggcctat ttcccatgat tccttcatat ttgcatatac
     3841 gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag
     3901 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat
     3961 gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt
     4021 tatatatctt gtggaaagga cgaaacaccg gagacggttg taaatgagca cacaaaatac
     4081 acatgctaaa atattatatt ctatgacctt tataaaatca accaaaatct tctttttaat
     4141 aactttagta tcaataatta gaatttttat gttccttttt gcaaactttt aataaaaatg
     4201 agcaaaataa aaaaacgcta gttttagtaa ctcgcgttgt tttcttcacc tttaataata
     4261 gctactccac cacttgttcc taagcggtca gctcctgctt caatcatttt ttgagcatct
     4321 tcaaatgttc taactccacc agctgcttta actaaagcat tgtctttaac aactgacttc
     4381 attagtttaa catcttcaaa tgttgcacct gattttgaaa atcctgttga tgttttaaca
     4441 aattctaatc cagcttcaac agctatttca caagctttca tgatttcttc ttttgttaat
     4501 aaacaatttt ccataataca tttaacaaca tgtgatccag ctgctttttt tacagctttc
     4561 atgtcttcta aaactaattc ataatttttg tcttttaatg caccaatatt taataccata
     4621 tcaatttctg ttgcaccatc tttaattgct tcagaaactt cgaatgcttt tgtagctgtt
     4681 gtgcatgcac ctagaggaaa acctacaaca tttgttattc ctacatttgt gccttttaat
     4741 aattctttac aatagcttgt tcaatatgaa ttaacacaaa ctgttgcaaa atcaaattca
     4801 attgcttcat cacataattg tttaatttca gctttcgtag catcttgttt taataatgtg
     4861 tgatctatat atttgtttag tttcattttt tctcctatat attcattttt aattttaatt
     4921 ctttaataat ttcgtctact ttaactttag cgttttgaac agattcacca acacctataa
     4981 aataaatttt tagtttaggt tcagttccac ttgggcgaac agcaaatcat gacttatctt
     5041 ctaaataaaa ttttagtaag tcttgtcctg gcatattata cattccatcg atgtagtctt
     5101 caacattaac aactttaagt ccagcaattt gagttaaggg tgttgctctc aatgatttca
     5161 ttaatggttc aatttttaat ttcttttctt ctggtttaaa attcaagttt aaagtgaaag
     5221 tgtaatatgc acccatttct ttaaataaat cttctaaata gtctactaat gttttatttt
     5281 gttttttata aaatcaagca gcctctgcta ttaatataga agcttgtatt ccatctttat
     5341 ctctagctga gtcatcaatt acatatccat aactttcttc ataagcaaaa acaaaattta
     5401 atccgttatc ttcttcttta gcaatttctc tacccattca tttaaatcca gttaaagttt
     5461 ttacaatatt aactccatat ttttcatgag cgattctatc acccaaatca cttgttacaa
     5521 aacttgaata tagagccgga ttttttggaa tgctatttaa gcgttttaga tttgataatt
     5581 ttcaatcaat taaaattggt cctgtttgat ttccatctaa tcttacaaaa tgaccatcat
     5641 gttttattgc cattccaaat ctgtcagcat ctgggtcatt cataataata atatctgcat
     5701 catgtttaat accatattca agcggtattt ttcatgcagg atcaaattct ggatttggat
     5761 ttacaacatt tttaaatgtt tcatcttcaa atgcatgctc ttcaacctca ataacgttat
     5821 atcctgattc acgtaatatt tttggggtaa atttagttcc tgttccatta actgcgctaa
     5881 aaataatttt taaatctttt ttagcttctt gctctttttt gtacgtctct gtttaagagc
     5941 tatgctggaa acagcatagc aagtttaaat aaggctagtc cgttatcaac ttgaaaaagt
     6001 ggcaccgagt cggtgctttt tttgaattct agatcttgag acaaatggca gtattcatcc
     6061 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     6121 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     6181 ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag tggctccggt
     6241 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     6301 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     6361 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     6421 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtgtcgtg acgcgggatc
     6481 cgccaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     6541 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     6601 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     6661 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     6721 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     6781 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     6841 caaggagccc gcgtggttcc tggccaccgt cggagtctcg cccgaccacc agggcaaggg
     6901 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     6961 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     7021 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     7081 tgcctgaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     7141 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     7201 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     7261 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     7321 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     7381 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     7441 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     7501 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     7561 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     7621 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     7681 cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7741 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     7801 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7861 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7921 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7981 tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa
     8041 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     8101 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8161 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     8221 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     8281 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     8341 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     8401 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     8461 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     8521 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     8581 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     8641 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     8701 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     8761 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     8821 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     8881 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     8941 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     9001 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     9061 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     9121 taaccctgat aaatgcttca ataatattga aaaaggaaga gt
//
