LOCUS       sequence-171163-       17746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fructose biosensor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17746)
  AUTHORS   Pini F, East AK, Appia-Ayme C, Tomek J, Karunakaran R, 
            Mendoza-Suarez M, Edwards A, Terpolilli JJ, Roworth J, Downie JA, 
            Poole PS
  TITLE     Bacterial Biosensors for in Vivo Spatiotemporal Mapping of Root 
            Secretion.
  JOURNAL   Plant Physiol. 2017 Jul;174(3):1289-1306. doi: 10.1104/pp.16.01302. 
            Epub 2017 May 11.
  PUBMED    28495892
REFERENCE   2  (bases 1 to 17746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.16.01302"; journalName: "Plant Physiol"; date: 
            "2017-07"; volume: "174"; issue: "3"; pages: "1289-1306"
COMMENT     Sequence Label: pLMB757
FEATURES             Location/Qualifiers
     source          1..17746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3296..3312)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3296..3312)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3309..3331)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3320..3336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3344..3350,3351..3368,3369..3374))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3389..3410
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3535..3553)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     oriT            3598..3706
                     /note="incP origin of transfer"
     promoter        3883..3987
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3988..4056,4057..4848)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4206..4225)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(5375..6574)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             6680..7330
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     rep_origin      7820..8531
                     /label=oriV
                     /note="incP origin of replication"
     CDS             9198..10346
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10398..11510)
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     CDS             complement(11689..12672)
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             complement(12687..13769)
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             complement(13818..14741)
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             complement(14754..16196)
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
ORIGIN
        1 ccccaccgcg gactctgact acaaccgtgc gcaaggcgca ttagggagga tgtatgtatg
       61 taatcgcctg cggcatcgtt gccggcttgg cggctgcggt ggccctgttg ggcttcacgc
      121 cgatgatgga ggcgcttgcc gccggcgaac gccgcaaggc actcgcgcaa tggacgcgga
      181 cgatgttcct ggtgctgctg cctgtcgtgc tgatgtgcgc gcccatcggg tccagcattt
      241 acgacgccgt gcaagcggac gctggcaagc ccatcgcttt ccacaacggc cggatcacgg
      301 tcgtcatggc cctggtcggc agcttggccg ttgtcctggt cgcggctgcg cgtgcggtgg
      361 tcaaccgcaa gcatgccagc ttctggttcg tcggctgggt gatggcgtcg gttttggccg
      421 gcggcgtcgg cgcgatcgcc agcgcgaagc aactggcgtt cctcggcgaa catagcggca
      481 tggtggcctt cggcttcttc cgcgaccagg tgaaggacat gcactgcgat gcggacgtga
      541 tcctggcccg gtgggatgaa aaggcgaact cgccggtggt ctaccgctgc ccgaaggcgt
      601 acctgctcaa caggttcgca tccgcgccct tcgtgccctg gccggactac accgaggggg
      661 aaagcgagga tctaggtagg gcgctcgcag cggccctgcg ggacgcgaaa aggtgagaaa
      721 agccgggcac tgcccggctt tatttttgct gctgcgcgtt ccaggccgcc cacactcgtt
      781 tgacctggct cgggctgcat ccgaccagct tggccgtctt ggcaatgctc gatccgccgg
      841 agcgaagcgt gatgatgcgg tcgtgcatgc cggcgtcacg tttgcggccg gtgtagcggc
      901 cggcggcctt cgccaactgg acaccctgac gttgacgctc gcgccgatcc tcgtagtcgt
      961 cgcgggccat ctgcaaggcg agcttcaaaa gcatgtcctg gacggattcc agaacgattt
     1021 tcgccactcc gttcgcctcg gcggccagct ccgacaggtc caccacgcca ggcacggcca
     1081 gcttggcccc tttggcccgg atcgacgcaa ccaggcgctc ggcctcggcc aacggcaagc
     1141 ggctgatgcg gtcgatcttc tccgcaacga cgacttcacc aggttgcagg tccgcgatca
     1201 tgcgcagcag ctcgggccgg tcggcgcgtg cgccggacgc cttctcgcgg tagatgccgg
     1261 cgacgtagta cccggcggcc cgcgtggccg ctacaaggct ctcctggcgt tcaagattct
     1321 gctcgtccgt actggcgcgc aggtagatgc gggcgacctt caaccttcgt ccctccggtt
     1381 gttgctctcg cgtcgccatt tccacggctc gacggcgtgc ggatcggacc agaggccgac
     1441 gcgcttgcct cgcgcctcct gttcgagccg cagcatttca gggtcggccg cgcggccgtg
     1501 gaagcgatag gcccacgcca tgccctggtg aaccatcgcg gcgttgacgt tgcgcggctg
     1561 cggcggccgg ctggccagct ccatgttgac ccacacggtg cccagcgtgc ggccgtaacg
     1621 gtcggtgtcc ttctcgtcga ccaggacgtg ccggcggaac accatgccgg ccagcgcctg
     1681 gcgcgcacgt tcgccgaagg cttgccgctt ttccggcgcg tcaatgtcca ccaggcgcac
     1741 gcgcaccggc tgcttgtcta ccagcacgtc gatggtgtcg ccgtcgatga tgcgcacgac
     1801 ctcgccgcgc agctcggccc atgccggcga ggcaacgacc aggacggcca gcgcggcagc
     1861 ggcgcgcagc atggcgtagc ttcggcgctt catgcgtggc cccattgctg atgatcgggg
     1921 tacgccaggt gcagcactgc atcgaaattg gccttgcagt agccgtccag cgccacccgc
     1981 gagccgaacg ccggcgaaag gtactcgacc aggccgggcc ggtcgcggac ctcgcgcccc
     2041 aggacgtgga tgcgccggcc gcgtgtgccg tcgggtccag gcacgaaggc cagcgcctcg
     2101 atgttgaagt cgatggatag aagttgtcgg tagtgcttgg ccgccctcat cgcgtccccc
     2161 ttggtcaaat tgggtatacc catttgggcc tagtctagcc ggcatggcgc attacagcaa
     2221 tacgcaattt aaatgcgcct agcgcatttt cccgacctta atgcgcctcg cgctgtagcc
     2281 tcacgcccac atatgtgcta atgtggttac gtgtatttta tggaggttat ccaatgagcc
     2341 gcctgacaat cgacatgacg gaccagcagc accagagcct gaaagccctg gccgccttgc
     2401 agggcaagac cattaagcaa tacgccctcg aacgtctgtt ccccggtgac gctgatgccg
     2461 atcaggcatg gcaggaactg aaaaccatgc tggggaaccg catcaacgat gggcttgccg
     2521 gcaaggtgtc caccaagagc gtcggcgaaa ttcttgatga agaactcagc ggggatcgcg
     2581 cttgacggcc tacatcctca cggctgaggc cgaagccgat ctacgcggca tcatccgcta
     2641 cacgcgccgg gagtggggcg cggcgcaggt gcgccgctat atcgctaagc tggaacaggg
     2701 catagccagg cttgccgccg gcgaaggccc gtttaaggac atgagcgaac tctttcccgc
     2761 gctgcggatg gcccgctgcg aacaccacta cgttttttgc ctgccgcgtg cgggcgaacc
     2821 cgcgttggtc gtggcgatcc tgcatgagcg catggacctc atgacgcgac ttgccgacag
     2881 gctcaagggc tgatttcagc cgctaaaaat cgcgccactc acaacgtcct gatggcgtac
     2941 ttacccaaag aacagctagg agaatcattt atgctcagca cacttccaca agctcatgca
     3001 actttcttga accgcatccg cgatgcggtc gcttccgatg ttcgcttccg cgctcttctg
     3061 atcggcggct cttacgttca cggaggactc gatgagcact ccgatttgga tttcgacatc
     3121 gttgttgagg acaactgcta cgcagatgtc ttgtctacac gcaaggattt tgccgaggca
     3181 ctgcccggct tcctcaacgc gttcaccggc gaacatgtag gagaaccgcg ccttctgatc
     3241 tgcctatatg gtccgccact gctacacatc gggggatcag cttggcgtaa tcatggtcat
     3301 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     3361 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     3421 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     3481 aacgcgcggg gagaggcggt ttgcgtattg gaattaattc ttgaagacga aagggcctcg
     3541 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag agcttatcgg
     3601 ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa
     3661 gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg
     3721 gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa
     3781 ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa
     3841 gatccgtcga tcgacccagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     3901 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3961 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4021 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4081 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4141 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4201 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     4261 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     4321 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     4381 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     4441 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     4501 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     4561 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     4621 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     4681 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4741 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     4801 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     4861 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4921 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4981 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgg
     5041 gggatcagga ccgctgccgg agcgcaaccc actcactaca gcagagccat gtagacaaca
     5101 tcccctcccc ctttccaccg cgtcagacgc ccgtagcagc ccgctacggg ctttttcatg
     5161 ccctgcccta gcgtccaagc ctcacggccg cgctcggcct ctctggcggc cttctggcgc
     5221 tcctgctgcg gcgtccgctc gtgggccgtg gcgcgggtcc gcgcgccggc ctcgtgcgcc
     5281 tggcgctcgc gggcgaggtc cagggcggcc gtcttcacgt tctgccttgc gcagatgaga
     5341 tagatccgtc aattccggcc tatcgtttcc acgatcagcg atcggctcgt tgccctgcgc
     5401 cgctccaaag cccgcgacgc agcgccggca ggcagagcaa gtagagggca gcgcctgcaa
     5461 tccatgccca cccgttccac gttgttatag aagccgcata gatcgccgtg aagaggaggg
     5521 gtccgacgat cgaggtcagg ctggtgagcg ccgccagtga gccttgcagc tgcccctgac
     5581 gttcctcatc cacctgcctg gacaacattg cttgcagcgc cggcattccg atgccacccg
     5641 aagcaagcag gaccatgatc gggaacgcca tccatccccg tgtcgcgaag gcaagcagga
     5701 tgtagcctgt gccgtcggca atcattccga gcatgagtgc ccgcctttcg ccgagccggg
     5761 cggctacagg gccggtgatc attgcctggg cgagtgaatg cagaatgcca aatgcggcaa
     5821 gcgaaatgcc gatcgtggtc gcgtcccagt gaaagcgatc ctcgccgaaa atgacccaaa
     5881 gcgcggccgg cacctgtccg acaagttgca tgatgaagaa gaccgccatc agggcggcga
     5941 cgacggtcat gccccgggcc caccggaacg aagcgagcgg gttgagagcc tcccggcgta
     6001 acggccggcg ttcgcctttg tgcgactccg gcaaaaggaa acagcccgtc aggaaattga
     6061 ggccgttcaa ggctgccgcg gcgaagaacg gagcgtgggg ggagaaaccg cccatcagcc
     6121 caccgagcac aggtcccgcg accatcccga acccgaaaca ggcgctcatg aagccgaagt
     6181 gccgcgcgcg ctcatcgcca tcagtgatat cggcaatata agcgccggct accgccccag
     6241 tcgccccggt gatgccggcc acgatccgcc cgatatagag aacccaaagg aaaggcgctg
     6301 tcgccatgat ggcgtagtcg acagtggcgc cggccagcga gacgagcaag attggccgcc
     6361 gcccgaaacg atccgacagc gcgcccagca caggtgcgca ggcaaattgc accaacgcat
     6421 acagcgccag cagaatgcca tagtgggcgg tgacgtcgtt cgagtgaacc agatcgcgca
     6481 ggaggcccgg cagcaccggc ataatcaggc cgatgccgac agcgtcgagc gcgacagtgc
     6541 tcagaattac gatcaggggt atgttgggtt tcacgtctgg cctccggacc agcctccgct
     6601 ggtccgattg aacgcgcgga ttctttatca ctgataagtt ggtggacata ttatgtttat
     6661 cagtgataaa gtgtcaagca tgacaaagtt gcagccgaat acagtgatcc gtgccgccct
     6721 ggacctgttg aacgaggtcg gcgtagacgg tctgacgaca cgcaaactgg cggaacggtt
     6781 gggggttcag cagccggcgc tttactggca cttcaggaac aagcgggcgc tgctcgacgc
     6841 actggccgaa gccatgctgg cggagaatca tacgcattcg gtgccgagag ccgacgacga
     6901 ctggcgctca tttctgatcg ggaatgcccg cagcttcagg caggcgctgc tcgcctaccg
     6961 cgatggcgcg cgcatccatg ccggcacgcg accgggcgca ccgcagatgg aaacggccga
     7021 cgcgcagctt cgcttcctct gcgaggcggg tttttcggcc ggggacgccg tcaatgcgct
     7081 gatgacaatc agctacttca ctgttggggc cgtgcttgag gagcaggccg gcgacagcga
     7141 tgccggcgag cgcggcggca ccgttgaaca ggctccgctc tcgccgctgt tgcgggccgc
     7201 gatagacgcc ttcgacgaag ccggtccgga cgcagcgttc gagcagggac tcgcggtgat
     7261 tgtcgatgga ttggcgaaaa ggaggctcgt tgtcaggaac gttgaaggac cgagaaaggg
     7321 tgacgattga tcaggaccgc tgccggagcg caacccactc actacagcag agccatgtag
     7381 acaacatccc ctcccccttt ccaccgcgtc agacgcccgt agcagcccgc tacgggcttt
     7441 ttcatgccct gccctagcgt ccaagcctca cggccgcgct cggcctctct ggcggccttc
     7501 tggcgctcct gctgcggcgt ccgctcgtgg gccgtggcgc gggtccgcgc gccggcctcg
     7561 tgcgcctggc gctcgcgggc gaggtccagg gcggccgtct tcacgttctg ccttgcgcag
     7621 atgagataga tcccggaatt gacggatcta gcgtggactc aaggctctcg cgaatggctc
     7681 gcgttggaaa ctttcattga cacttgaggg gcaccgcagg gaaattctcg tccttgcgag
     7741 aaccggctat gtcgtgctgc gcatcgagcc tgcgcccttg gcttgtctcg cccctctccg
     7801 cgtcgctacg gggcttccag cgcctttccg acgctcaccg ggctggttgc cctcgccgct
     7861 gggctggcgg ccgtctatgg ccctgcaaac gcgccagaaa cgccgtcgaa gccgtgtgcg
     7921 agacaccgcg gccgccggcg ttgtggatac ctcgcggaaa acttggccct cactgacaga
     7981 tgaggggcgg acgttgacac ttgaggggcc gactcacccg gcgcggcgtt gacagatgag
     8041 gggcaggctc gatttcggcc ggcgacgtgg agctggccag cctcgcaaat cggcgaaaac
     8101 gcctgatttt acgcgagttt cccacagatg atgtggacaa gcctggggat aagtgccctg
     8161 cggtattgac acttgagggg cgcgactact gacagatgag gggcgcgatc cttgacactt
     8221 gaggggcaga gtgctgacag atgaggggcg cacctattga catttgaggg gctgtccaca
     8281 ggcagaaaat ccagcatttg caagggtttc cgcccgtttt tcggccaccg ctaacctgtc
     8341 ttttaacctg cttttaaacc aatatttata aaccttgttt ttaaccaggg ctgcgccctg
     8401 tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct cccggcccgc
     8461 taacgcgggc ctcccatccc cccaggggct gcgcccctcg gccgcgaacg gcctcacccc
     8521 aaaaatggca gcgctggcag tccttgccat tgccgggatc ggggcagtaa cgggatgggc
     8581 gatcagcccg agcgcgacgc ccggaagcat tgacgtgccg caggtgctgg catcgacatt
     8641 cagcgaccag gtgccgggca gtgagggcgg cggcctgggt ggcggcctgc ccttcacttc
     8701 ggccgtcggg gcattcacgg acttcatggc ggggccggca atttttacct tgggcattct
     8761 tggcatagtg gtcgcgggtg ccgtgctcgt gttcgggggt gaattaattc cccggatcga
     8821 tccgtcagct tcacgctgcc gcaagcactc agggcgcaag ggctgctaaa ggaagcggaa
     8881 cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc
     8941 tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac
     9001 atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg
     9061 ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc
     9121 aaggatctga tggcgcaggg gatcaagatc gacggatcga tccggggaat tccggggcaa
     9181 tcccgcaagg agggtgaatg aatcggacgt ttgaccggaa ggcatacagg caagaactga
     9241 tcgacgcggg gttttccgcc gaggatgccg aaaccatcgc aagccgcacc gtcatgcgtg
     9301 cgccccgcga aaccttccag tccgtcggct cgatggtcca gcaagctacg gccaagatcg
     9361 agcgcgacag cgtgcaactg gctccccctg ccctgcccgc gccatcggcc gccgtggagc
     9421 gttcgcgtcg tctcgaacag gaggcggcag gtttggcgaa gtcgatgacc atcgacacgc
     9481 gaggaactat gacgaccaag aagcgaaaaa ccgccggcga ggacctggca aaacaggtca
     9541 gcgaggccaa gcaggccgcg ttgctgaaac acacgaagca gcagatcaag gaaatgcagc
     9601 tttccttgtt cgatattgcg ccgtggccgg acacgatgcg agcgatgcca aacgacacgg
     9661 cccgctctgc cctgttcacc acgcgcaaca agaaaatccc gcgcgaggcg ctgcaaaaca
     9721 aggtcatttt ccacgtcaac aaggacgtga agatcaccta caccggcgtc gagctgcggg
     9781 ccgacgatga cgaactggtg tggcagcagg tgttggagta cgcgaagcgc acccctatcg
     9841 gcgagccgat caccttcacg ttctacgagc tttgccagga cctgggctgg tcgatcaatg
     9901 gccggtatta cacgaaggcc gaggaatgcc tgtcgcgcct acaggcgacg gcgatgggct
     9961 tcacgtccga ccgcgttggg cacctggaat cggtgtcgct gctgcaccgc ttccgcgtcc
    10021 tggaccgtgg caagaaaacg tcccgttgcc aggtcctgat cgacgaggaa atcgtcgtgc
    10081 tgtttgctgg cgaccactac acgaaattca tatgggagaa gtaccgcaag ctgtcgccga
    10141 cggcccgacg gatgttcgac tatttcagct cgcaccggga gccgtacccg ctcaagctgg
    10201 aaaccttccg cctcatgtgc ggatcggatt ccacccgcgt gaagaagtgg cgcgagcagg
    10261 tcggcgaagc ctgcgaagag ttgcgaggca gcggcctggt ggaacacgcc tgggtcaatg
    10321 atgacctggt gcattgcaaa cgctagggcc ttgtggggtc agttccggct gggggttcag
    10381 cagccacctg caggtcatca actatcaaac gcttcggtta agcttaaagc acaccctttc
    10441 tgcgtcctcg tattgacgcg acgtaaaatt tcaacgagca cgccgggata cttaccatat
    10501 tctctgctaa ttatcccgac atcatcggta acaataaatg ctggataact ggttgctgac
    10561 gcatccatat aactcatcaa ccccggcgtt ccatcaggta caggtttcaa cgtttcagga
    10621 tcaagcgctc gcgcatatac ccacggcgga acatgtttac gctgcatttc atcctcaaag
    10681 aaacaagtgt tgagttcaac ttgattaaat atatctcgga tctgactaat atcactgaga
    10741 ttgaaagtat caaataaaag atgattgaaa tcatcacgtt tcagagattc tttttcgtaa
    10801 cttttccagc cgcctccggt tatgatataa aggcttttat ctccagaaaa tgagattttt
    10861 ttatctttca tataatggca gagtaaataa ataaagtatg gcgaaccaat aagacaaaga
    10921 tctttccctt gattttttat tcgttcaaga ctattcaatg ttttaacaaa atctattcgt
    10981 tcttctgtta cggtaaatgt cgtaggatat aacaattcca ccaaactcat aacatattta
    11041 aaccaaatat tatgagcatt aaatctatct ggtcccaaat tgactaattc tatttgatga
    11101 tcaaaccaac taccaacata tttcatgcca taactcacag agcctaagag tctctcaata
    11161 cttaatctgt cacgcgccac ctgacttttt aaaccattcg tgccgctact ggtaaaccaa
    11221 ctttcaatct cgttttcctg agaagttaat aagcgagtaa acttaaaaac cgatgttggg
    11281 aatacaggta tgtcatcaat ttccgtaata ttgtcatcta ctttgtgtgc ctgacagtag
    11341 tgacgatatt ctcgacaatg tttataatga ttacgaaatg catcaagcac aagtttcttt
    11401 ctgatttttt cctgctcgtc gtaagaccac actaatggat cgctcgaaaa aatcaaatca
    11461 tcaatttctg agcttgctgt aatttcttgt ttatcaacat atgaagtcat acctgtttta
    11521 ctcctcaaga taatattaga aagtatggca gcactgctgt catactcttt tatacccttc
    11581 atctttcaag ctgctgcttt gttggctgct ttcactcacc ccagtcacat agttatctat
    11641 gctcctgggg attcgttcac ttgccgcctc gctgcaactc gaaatctatt aggtatattc
    11701 catgtggtac ttcttaatat tatcatcaac aatattgatt acattttttt ggctcatcaa
    11761 atcattcatt ggttcaaagg acagcaatac acttttcgca ccacactttt caattgccaa
    11821 cttagccgca gttatacact ccgtataatt tccgacagcg ttttctgcaa ttatttcttc
    11881 aagtttattt tcgaaatttt cattagggtg catttcaaga acataatcac taataaatgc
    11941 acgcgtctct tgtttagctt tattactatc ttcgttatag ttaactaata tcattaactg
    12001 atggtctatc tctgataggt caacgtcata tttatccgca acggctttat atctttcagc
    12061 atattcatat ctaacatcat tagaatcatc ccacttaaag atgagaggaa tacctttttt
    12121 ggccgcccac tcaacaatat gatgactggt tgctgttaca tatttccgag gtccgcctgg
    12181 cgtataagca tggggattta cagatatttt agggaagcta taaaaatcgt tatctggatt
    12241 acaatagcct gttgttaaag catcgttaat gatttcataa cactcttcaa atagttgctg
    12301 ttgatattca accgggcgat taaaaaaatg catttcatct tttttttcgc aatcactaaa
    12361 ccctaaaata aatctccctt cacttaactg atccaataag caagcttcct ccgctatgcg
    12421 gacaggatga tgagttgtaa tgatgtgatt taatgaacca attttaattt tctctgttaa
    12481 accgagcaga aaaccagaaa cagtcagagg agcgccgaca acaccattat ctgaaaaatg
    12541 attttcatac actaaaatct gttcaaaatt caacttatca acatactccg ttatttcctg
    12601 catgcgaact atactttgtt cttgaacagt tgttgaattg atgaagttaa ggaagaacaa
    12661 tccaaatttc atttctttct ccttagctaa tataatagcg aacgttgttt ttctttaaga
    12721 aatggcatga catcagactg gaagagcttc atggaagcaa taatttcgtc tactgttcca
    12781 ttagcttcaa atccacaaca aatatttgat attcctgtag catcaatgtc tttttgaatt
    12841 atgtcaatac attcctgcgg cgttcccacg ggattgattt cgtaactgta atcaatacgg
    12901 cgattagtat ctttatgtcc ttttaataca aagtcacgcc actgcccttt attgaaatca
    12961 taacctcttg tttggtctga atcatcaaaa atagtcgtag cattcacata agaatcatac
    13021 caatgcccca gaaatttccg gcaaatctct ttcgctttaa ttgagtcatg atctacagat
    13081 gttatatatg ataagcaatg gtcgatatta tgaatatcgt gcccatattc ttgagccact
    13141 tcattataaa gctcaagttg tgctttcttt tcgttagtat ttataatcca acttaatatc
    13201 atcggtaggc caaattgagc agcccactca gtcgtcgaag ctgattcagc caccacataa
    13261 accggtgcgc cacctctgct atacgccgcg gggtttactt ttaccttatg gaacttgata
    13321 tgttcattat cagcttccat atatccctct gtcatgccat tctttatcag cccgtaccag
    13381 cattccgcta aggcgcgact gttattcata tctgtgccga atacgcgaaa gtccttgttg
    13441 taaagccctc ggcaaatacc aaaccgaaat cgtccttttg acatttgatc caataaattc
    13501 acatcttcaa gttggcgtac tggatgggct gtgggaagaa caatagcggc agttcctaca
    13561 ttcaattttt tagtcgcgcc aagtaaatat gcagcagcga cataagggtt accaagcaaa
    13621 ccaaactccg tgaaatgatg ctccagtaac catacggtat caaaaccaca ctcctcagag
    13681 atgcgaccta atttaaccaa acgtttcatt acctctgttt gagaaaattg gggaggttgg
    13741 tatgtaagca aaaagtttcc aaatttcata gagagtcctt atattgctat ttgagtgata
    13801 gaatatctca atagatttta agacagagaa attgcttgat tttcaatctc aattctcatt
    13861 cggcgttcat tgactgtcgc aatagttaaa tgttcaaatg acggttcagt aatatcaaca
    13921 tcaatatcca gatgatcatt atccatcgcg atagcggctt tcgtaaccga ttgataaaaa
    13981 ttgcgcagga ccactaaatt ttcactcaag tcatgcgaac ttcctaacaa agaatatatc
    14041 ttgcatcgat tactacgaat atttgataac aatgtgataa cttcatcttg cttgacccaa
    14101 ttatcgttat ttgcagtaaa agcaataaac ggtatatcaa gatacatcat gttattaatt
    14161 gtagaagcta aatcttccca accaaaatca agacaatctc tcgcaaagac ttcagcaccc
    14221 aatttatggc cttcaaaatc tagattattc ggcaattcat taatgggtag actgagataa
    14281 tcaaacccta aagctctttc aagagaatat cttaagttaa caacaccgac tgcggtgatt
    14341 aaaaacgaag cattgatttc agataggctt gcataagcta tccgcgcaga taagcttgaa
    14401 gccaacatac cgaagttatt tatttttcgt gtagttaacc aatcaaccac tgctaacaag
    14461 ctctgctttc ctatagacat tgtaaattca tcaattgtcc ctgaactcaa tccaacgtgg
    14521 tgaagcgaat catagcggat cacatgaaat ccattccgcg ataaatattc cgccagacca
    14581 gcaaaatgat ccatcctgcg ggcaaaacca gacgcaataa taatggcatt ctttctcttt
    14641 gggctgtttt cttctggcag cgtttcccaa acatgaattt ttttatttcc ttcaacacaa
    14701 ataacgtggt cgatggtttt atattttgat tcattttcca tactttttac ctattatggg
    14761 acaaatacaa ggaacttatc ttcttccagg aatcgagtct gttctatttc aaccgcaaca
    14821 tccttagccg tatagttaga tggcctttca tgagaaatat atgtcactaa tcgttgcaac
    14881 ggtctcattc cgtcatgaga tccaccaact cgaaatatgt tattcattcc tgcttctaca
    14941 atcctttccg caccttttaa tgctaacgca tctcgatatt taaatgatga ctcccaagga
    15001 aaaatagata tggtttgcgt cttatttttt tgaacataag gcaatatttg ctcaatatta
    15061 tcgacgtgat gaaggtacac acatctgcca agtggttgat taaattccac acctgcattt
    15121 gactcaataa tcatccaacg ttgatgaata tccacctcta cttttaatcc agcaaacaag
    15181 ctttcttttt gaactaaaga ataggccgcc ttttcatcaa aatctttttt ggcattcggt
    15241 aatatatgcg catatagatt aagtttttct atcaacgcta acttaaattc ctcataatga
    15301 tttcccatgt aatatatgtt ttgggcagaa aaacaagctc gctgatcgta aaaacaaaca
    15361 tcatgagccg cacctgtcgc tgcggacgtc aaatcaacag gattatcgat aatgcaaaga
    15421 ctctttttag aaccaaattt aatcacatca gcataagatg gcgcatgctc taccgcccaa
    15481 ttaatcgcat ctggccctcc ccaagcgaca ataacatccg catgttgcat aatttctttt
    15541 gcgagtgatg tatcaccttg gtggggccaa tatataacag ataaagagcg cgttatcgga
    15601 tgattagggt ctacatcaat aaaacttaac gctaatgcat tagcggtaaa aggatcggtt
    15661 gacgatgttt ttataataca ctgattctta gttaaaattg cgcgtaatat agacatgatc
    15721 ccagataatg gaacattacc tgccaacaga tgtacagatt tacctttcgg aaaagcccga
    15781 acataacttt catcctgagg tagccattca tccatgatat ggcgagaacc aagttcattt
    15841 tctacaacat cataaaggcc gcctttagaa cataaaatca tagatatcca attggcctct
    15901 agcttagcca tttcttctga atatcccata tattttttta agtcacgaat gtatgtcctg
    15961 cgtcttgagt attcttcatt tttccatctt tgccctaccg tatagagaaa attgacaatg
    16021 ttatgcaacc gtaattcgtt atttccatta caatcaataa tgttttttac atgagagtca
    16081 ttcaatattg gcaggtaaac actattatca ccaaaattaa tggattgcac taaatcatca
    16141 ccttcgggaa agatttcaac ctggccgtta ataatgaatg aaattttttt agtcatacgt
    16201 atcctccaag cctgaattcg tttaaacgga tccctcgaga acagacttct tcatggaact
    16261 cctcccagag tttgccggcg ccgcccctgc ggggccggaa gcttaagata tgacacttcg
    16321 ccgtggatcg ctaatgcctc ccagcggtcc cgtgcccttg acgacggcaa ttaagagcga
    16381 aaagaaatcg gcttgtcaat aaataaatcc aattgaatta ttaattcgat gtggcatgct
    16441 ccatcgaatt cgggcatagg ccaacgatcg ggaggagcgg ccatgtcgac cttggatgga
    16501 ccggaacaca cgccggtccc gccaccaatt ctgaatccgg ccggaggtgc gaaccaggtc
    16561 agggtgcggg cctataacga acggctcgtg ctgtcgctgg tgcgtctcta cggcgcgctg
    16621 tcgaaggccg atatcgcgcg ccgcagcggg ctgtcggcac agaccgtctc cgtgataatg
    16681 cgggtgctgg agaaggaagg gctgctgtcg cgcggggagc cggtgcgcgg ccgtgtcggc
    16741 cagccgtcga tcccgatgca tatcaatccc gatgccgtct attccttcgg cctgaagatg
    16801 ggccggcgca gcgccgatct ggtgctgatg gatttcgtcg gccgcatccg catgcagttg
    16861 caccggacct atgcctaccc actgccggat gaaatcctcg ccttcgtcat ctcgggcatc
    16921 cgggagctgg aagagcggct cgacgacaag cagcgctgcc gtattgccgg cctcggcatc
    16981 gccgcgcctt tcgagctctg gaattgggcc gaagaggtgg gggcgccggc tggcgccatg
    17041 gaggtctggc gggagatcga cctgcaggcc gacatcgcct cccgcgtctc gcatccggtc
    17101 ttcatgcaga acgatgcgac cagcgcctgc ggggccgaac tcgtcttcgg cgtcgggccg
    17161 tcctatccgg atttcgtcta tttcttcatc ggctccttca tcggcggcgg catcgtcctc
    17221 aattcggcga tcttttccgg ccgcaccggc acggcaggcg cgatcggccc cctgcccgtg
    17281 cgcggcaaga acggcgagac gaagcagttg ctcgaaatcg cctcgatctt cgtgctcgaa
    17341 aacatgctgc gcgagcgtgg catagatccc gagccgctct ggtattcggc cgacgactgg
    17401 gtggatttcg gcgagccgat ggaagcctgg atccaggata gcgccaaggc gctggcgcag
    17461 gcgatcgtcg ccgccgcctc gatcctcgat ttcagcgcgg ctgtgatcga cggcggcttt
    17521 cccagctggg tgcgcagccg cgtcgtgcag gccaccatcg acgaagcggc caagctcgac
    17581 ctgcagggcg tcgtcatgcc cgaaatcatc gagggcgcgg tcggcgccca ggcgcgcgcc
    17641 atcggcggcg ccagcctgcc gatcttcgcg cgttacctca ccgaccagaa cgtactcttc
    17701 aaggaggtag accatgctga aaggacttga tccgctgttg aggtac
//
