LOCUS       sequence-171164-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of dcas9 D10A H840A from a TetR-regulated uvtetO 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Singh AK, Carette X, Potluri LP, Sharp JD, Xu R, Prisic S, Husson RN
  TITLE     Investigating essential gene function in Mycobacterium tuberculosis 
            using an efficient CRISPR interference system.
  JOURNAL   Nucleic Acids Res. 2016 Jul 12. pii: gkw625.
  PUBMED    27407107
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jul 12. pii: gkw625."
COMMENT     Sequence Label: pRH2502
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gagcacgatc cgctcgaggc atgcgaagga gatatacata tggacaagaa gtacagcatc
       61 ggcctggcga tcggcacgaa ctcggtgggc tgggcggtca tcacggacga gtacaaggtc
      121 ccctccaaga agttcaaggt cctgggcaac accgaccggc acagcatcaa gaagaacctg
      181 atcggcgccc tgctgttcga ctccggcgag accgccgagg cgacgcgcct gaagcggacc
      241 gcgcggcgcc gatacacgcg gcgcaagaac cgcatctgct acctgcagga aatcttctcg
      301 aacgagatgg ccaaggtgga cgacagcttc ttccaccggc tggaggagtc cttcctggtg
      361 gaggaggaca agaagcacga gcgccacccg atcttcggca acatcgtgga cgaggtggcc
      421 taccacgaga agtaccccac gatctaccac ctgcgcaaga agctggtgga cagcaccgac
      481 aaggcggacc tgcggctgat ctacctggcc ctggcgcaca tgatcaagtt ccgcggccac
      541 ttcctgatcg agggcgacct gaacccggac aactcggacg tggacaagct gttcatccag
      601 ctggtgcaga cctacaacca gctgttcgag gagaacccca tcaacgcctc cggcgtggac
      661 gccaaggcga tcctgtcggc
//
