LOCUS       sequence-171165-         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of dcas9 D10A H840A from a TetR-regulated uvtetO 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Singh AK, Carette X, Potluri LP, Sharp JD, Xu R, Prisic S, Husson RN
  TITLE     Investigating essential gene function in Mycobacterium tuberculosis 
            using an efficient CRISPR interference system.
  JOURNAL   Nucleic Acids Res. 2016 Jul 12. pii: gkw625.
  PUBMED    27407107
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jul 12. pii: gkw625."
COMMENT     Sequence Label: pRH2502
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agccggcctt cctgtcgggc gagcagaaga aggcgatcgt ggacctgctg ttcaagacca
       61 accgcaaggt cacggtgaag cagctgaagg aggactactt caagaagatc gagtgtttcg
      121 actcggtcga gatcagcggc gtggaggacc gcttcaacgc cagcctgggc acctaccacg
      181 acctgctgaa gatcatcaag gacaaggact tcctggacaa cgaggagaac gaggacatcc
      241 tggaggacat cgtcctgacc ctgacgctgt tcgaggaccg cgagatgatc gaggagcggc
      301 tgaagacgta cgcccacctg ttcgacgaca aggtgatgaa gcagctgaag cggcgccgat
      361 acaccggctg gggccgcctg tcgcggaagc tgatcaacgg catccgggac aagcagtccg
      421 gcaagaccat cctggacttc ctgaagtcgg acggcttcgc gaaccgcaac ttcatgcagc
      481 tgatccacga cgacagcctg accttcaagg aggacatcca gaaggcccaa gtgagcggcc
      541 agggcgactc cctgcacgag cacatcgcca acctggcggg ctcgccggcg atcaagaagg
      601 gcatcctgca gaccgtcaag gtcgtggacg agctggtcaa ggtgatgggc cgccacaagc
      661 ccgagaacat cgtgatcgag atggcccggg agaaccagac cacgcagaa
//
