LOCUS       sequence-171185-       17365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Formate biosensor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17365)
  AUTHORS   Pini F, East AK, Appia-Ayme C, Tomek J, Karunakaran R, 
            Mendoza-Suarez M, Edwards A, Terpolilli JJ, Roworth J, Downie JA, 
            Poole PS
  TITLE     Bacterial Biosensors for in Vivo Spatiotemporal Mapping of Root 
            Secretion.
  JOURNAL   Plant Physiol. 2017 Jul;174(3):1289-1306. doi: 10.1104/pp.16.01302. 
            Epub 2017 May 11.
  PUBMED    28495892
REFERENCE   2  (bases 1 to 17365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.16.01302"; journalName: "Plant Physiol"; date: 
            "2017-07"; volume: "174"; issue: "3"; pages: "1289-1306"
COMMENT     Sequence Label: pLMB710
FEATURES             Location/Qualifiers
     source          1..17365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4433..4449)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4433..4449)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4446..4468)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4457..4473
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4481..4487,4488..4505,4506..4511))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4526..4547
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4672..4690)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     oriT            4735..4843
                     /note="incP origin of transfer"
     promoter        5020..5124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5125..5193,5194..5985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5343..5362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(6512..7711)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             7817..8467
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     rep_origin      8957..9668
                     /label=oriV
                     /note="incP origin of replication"
     CDS             10335..11483
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11535..12647)
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     CDS             complement(12826..13809)
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             complement(13824..14906)
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             complement(14955..15878)
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             complement(15891..17333)
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
ORIGIN
        1 gatccagatc ggcgacgatg gctcgggtgc gtgctgcaat atcgccttcg gcgatatgaa
       61 tggtcataag gcagccctcc cagccgcttg ctcgttgcga aatagcgggc ctcccccagc
      121 cggttgctat tccgcaacca ccatctcagg gaagcagccg gcgatcaata aagctatctc
      181 gttgcttgat aggttttttc tatcaaagtt cttcatcttg tacgagtact ctcgcttcat
      241 gcagcaaggc cgagacaagc ggcgtgaagg gctcgcgata aggcgcaaca aggccgacca
      301 gatgatgcgc ctccggctcg acgatcggga tcatgcggat ttccactgga aacccgaagg
      361 atttggcgac gttgcgcggc atgatgctcg cccagcgtcc agtcctgaca tgcgagaaca
      421 gcacgatcat cgagttggat tcgagcgtcg gatgcgctgt ggcgcctgct tccgtcaagt
      481 ggcggttgat gatgcggcgg ttctgcatgt cggcagtcaa tagacaaagc cgaaggtcgc
      541 cgatctcccg ccaggtcacg ttgtcgcgat cagatagcgg gctgccggca gccgtgatca
      601 gattatagcg ctcggcataa aggggaacgg tcgtgacgcg gccgagcggc tcgttttcga
      661 gataggtgat gccggcatcg atttcgaggt tttcgagcat gctcagcacc tgcagcgaat
      721 tccgcgagac gatcgagaag gtcacgccgg gatgccgctc ctgaaatggc gtggtgatgc
      781 gcgacagcat ggcaagcgcg gtcggaatgg cggcgaggcg gatgtggccg gaaagaccgc
      841 ggcgcgcggc gcgcatctcc tcgcgcatgg tgcgggcatc gccgacgatg cgtcgggccc
      901 attcgagcac gcgctgcccc tcaggcgtca gcccctggaa ccgcgaaccg cgctgcacca
      961 gcatgacgcc aagctgatcc tcgagctgcc ggatggcagc cgaaagcgtt ggctgcgaga
     1021 tcccgcactc ctccgcggcg cggccaaaat gcttttcatt ggcaagggcg atgaagaatt
     1081 ccagcttgtc gatcatccgg tctcccgatc cggcatgaca tcatccgggc caggtacccc
     1141 caccgcggac tctgactaca accgtgcgca aggcgcatta gggaggatgt atgtatgtaa
     1201 tcgcctgcgg catcgttgcc ggcttggcgg ctgcggtggc cctgttgggc ttcacgccga
     1261 tgatggaggc gcttgccgcc ggcgaacgcc gcaaggcact cgcgcaatgg acgcggacga
     1321 tgttcctggt gctgctgcct gtcgtgctga tgtgcgcgcc catcgggtcc agcatttacg
     1381 acgccgtgca agcggacgct ggcaagccca tcgctttcca caacggccgg atcacggtcg
     1441 tcatggccct ggtcggcagc ttggccgttg tcctggtcgc ggctgcgcgt gcggtggtca
     1501 accgcaagca tgccagcttc tggttcgtcg gctgggtgat ggcgtcggtt ttggccggcg
     1561 gcgtcggcgc gatcgccagc gcgaagcaac tggcgttcct cggcgaacat agcggcatgg
     1621 tggccttcgg cttcttccgc gaccaggtga aggacatgca ctgcgatgcg gacgtgatcc
     1681 tggcccggtg ggatgaaaag gcgaactcgc cggtggtcta ccgctgcccg aaggcgtacc
     1741 tgctcaacag gttcgcatcc gcgcccttcg tgccctggcc ggactacacc gagggggaaa
     1801 gcgaggatct aggtagggcg ctcgcagcgg ccctgcggga cgcgaaaagg tgagaaaagc
     1861 cgggcactgc ccggctttat ttttgctgct gcgcgttcca ggccgcccac actcgtttga
     1921 cctggctcgg gctgcatccg accagcttgg ccgtcttggc aatgctcgat ccgccggagc
     1981 gaagcgtgat gatgcggtcg tgcatgccgg cgtcacgttt gcggccggtg tagcggccgg
     2041 cggccttcgc caactggaca ccctgacgtt gacgctcgcg ccgatcctcg tagtcgtcgc
     2101 gggccatctg caaggcgagc ttcaaaagca tgtcctggac ggattccaga acgattttcg
     2161 ccactccgtt cgcctcggcg gccagctccg acaggtccac cacgccaggc acggccagct
     2221 tggccccttt ggcccggatc gacgcaacca ggcgctcggc ctcggccaac ggcaagcggc
     2281 tgatgcggtc gatcttctcc gcaacgacga cttcaccagg ttgcaggtcc gcgatcatgc
     2341 gcagcagctc gggccggtcg gcgcgtgcgc cggacgcctt ctcgcggtag atgccggcga
     2401 cgtagtaccc ggcggcccgc gtggccgcta caaggctctc ctggcgttca agattctgct
     2461 cgtccgtact ggcgcgcagg tagatgcggg cgaccttcaa ccttcgtccc tccggttgtt
     2521 gctctcgcgt cgccatttcc acggctcgac ggcgtgcgga tcggaccaga ggccgacgcg
     2581 cttgcctcgc gcctcctgtt cgagccgcag catttcaggg tcggccgcgc ggccgtggaa
     2641 gcgataggcc cacgccatgc cctggtgaac catcgcggcg ttgacgttgc gcggctgcgg
     2701 cggccggctg gccagctcca tgttgaccca cacggtgccc agcgtgcggc cgtaacggtc
     2761 ggtgtccttc tcgtcgacca ggacgtgccg gcggaacacc atgccggcca gcgcctggcg
     2821 cgcacgttcg ccgaaggctt gccgcttttc cggcgcgtca atgtccacca ggcgcacgcg
     2881 caccggctgc ttgtctacca gcacgtcgat ggtgtcgccg tcgatgatgc gcacgacctc
     2941 gccgcgcagc tcggcccatg ccggcgaggc aacgaccagg acggccagcg cggcagcggc
     3001 gcgcagcatg gcgtagcttc ggcgcttcat gcgtggcccc attgctgatg atcggggtac
     3061 gccaggtgca gcactgcatc gaaattggcc ttgcagtagc cgtccagcgc cacccgcgag
     3121 ccgaacgccg gcgaaaggta ctcgaccagg ccgggccggt cgcggacctc gcgccccagg
     3181 acgtggatgc gccggccgcg tgtgccgtcg ggtccaggca cgaaggccag cgcctcgatg
     3241 ttgaagtcga tggatagaag ttgtcggtag tgcttggccg ccctcatcgc gtcccccttg
     3301 gtcaaattgg gtatacccat ttgggcctag tctagccggc atggcgcatt acagcaatac
     3361 gcaatttaaa tgcgcctagc gcattttccc gaccttaatg cgcctcgcgc tgtagcctca
     3421 cgcccacata tgtgctaatg tggttacgtg tattttatgg aggttatcca atgagccgcc
     3481 tgacaatcga catgacggac cagcagcacc agagcctgaa agccctggcc gccttgcagg
     3541 gcaagaccat taagcaatac gccctcgaac gtctgttccc cggtgacgct gatgccgatc
     3601 aggcatggca ggaactgaaa accatgctgg ggaaccgcat caacgatggg cttgccggca
     3661 aggtgtccac caagagcgtc ggcgaaattc ttgatgaaga actcagcggg gatcgcgctt
     3721 gacggcctac atcctcacgg ctgaggccga agccgatcta cgcggcatca tccgctacac
     3781 gcgccgggag tggggcgcgg cgcaggtgcg ccgctatatc gctaagctgg aacagggcat
     3841 agccaggctt gccgccggcg aaggcccgtt taaggacatg agcgaactct ttcccgcgct
     3901 gcggatggcc cgctgcgaac accactacgt tttttgcctg ccgcgtgcgg gcgaacccgc
     3961 gttggtcgtg gcgatcctgc atgagcgcat ggacctcatg acgcgacttg ccgacaggct
     4021 caagggctga tttcagccgc taaaaatcgc gccactcaca acgtcctgat ggcgtactta
     4081 cccaaagaac agctaggaga atcatttatg ctcagcacac ttccacaagc tcatgcaact
     4141 ttcttgaacc gcatccgcga tgcggtcgct tccgatgttc gcttccgcgc tcttctgatc
     4201 ggcggctctt acgttcacgg aggactcgat gagcactccg atttggattt cgacatcgtt
     4261 gttgaggaca actgctacgc agatgtcttg tctacacgca aggattttgc cgaggcactg
     4321 cccggcttcc tcaacgcgtt caccggcgaa catgtaggag aaccgcgcct tctgatctgc
     4381 ctatatggtc cgccactgct acacatcggg ggatcagctt ggcgtaatca tggtcatagc
     4441 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     4501 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     4561 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     4621 gcgcggggag aggcggtttg cgtattggaa ttaattcttg aagacgaaag ggcctcgtga
     4681 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagagc ttatcggcca
     4741 gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa
     4801 ggaacacccg ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat
     4861 acaccaagga aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga
     4921 tggatatacc gaaaaaatcg ctataatgac cccgaagcag ggttatgcag cggaaaagat
     4981 ccgtcgatcg acccaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5041 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5101 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5161 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5221 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5281 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5341 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     5401 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5461 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5521 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5581 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5641 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     5701 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5761 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5821 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5881 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5941 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6001 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6061 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6121 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcggggg
     6181 atcaggaccg ctgccggagc gcaacccact cactacagca gagccatgta gacaacatcc
     6241 cctccccctt tccaccgcgt cagacgcccg tagcagcccg ctacgggctt tttcatgccc
     6301 tgccctagcg tccaagcctc acggccgcgc tcggcctctc tggcggcctt ctggcgctcc
     6361 tgctgcggcg tccgctcgtg ggccgtggcg cgggtccgcg cgccggcctc gtgcgcctgg
     6421 cgctcgcggg cgaggtccag ggcggccgtc ttcacgttct gccttgcgca gatgagatag
     6481 atccgtcaat tccggcctat cgtttccacg atcagcgatc ggctcgttgc cctgcgccgc
     6541 tccaaagccc gcgacgcagc gccggcaggc agagcaagta gagggcagcg cctgcaatcc
     6601 atgcccaccc gttccacgtt gttatagaag ccgcatagat cgccgtgaag aggaggggtc
     6661 cgacgatcga ggtcaggctg gtgagcgccg ccagtgagcc ttgcagctgc ccctgacgtt
     6721 cctcatccac ctgcctggac aacattgctt gcagcgccgg cattccgatg ccacccgaag
     6781 caagcaggac catgatcggg aacgccatcc atccccgtgt cgcgaaggca agcaggatgt
     6841 agcctgtgcc gtcggcaatc attccgagca tgagtgcccg cctttcgccg agccgggcgg
     6901 ctacagggcc ggtgatcatt gcctgggcga gtgaatgcag aatgccaaat gcggcaagcg
     6961 aaatgccgat cgtggtcgcg tcccagtgaa agcgatcctc gccgaaaatg acccaaagcg
     7021 cggccggcac ctgtccgaca agttgcatga tgaagaagac cgccatcagg gcggcgacga
     7081 cggtcatgcc ccgggcccac cggaacgaag cgagcgggtt gagagcctcc cggcgtaacg
     7141 gccggcgttc gcctttgtgc gactccggca aaaggaaaca gcccgtcagg aaattgaggc
     7201 cgttcaaggc tgccgcggcg aagaacggag cgtgggggga gaaaccgccc atcagcccac
     7261 cgagcacagg tcccgcgacc atcccgaacc cgaaacaggc gctcatgaag ccgaagtgcc
     7321 gcgcgcgctc atcgccatca gtgatatcgg caatataagc gccggctacc gccccagtcg
     7381 ccccggtgat gccggccacg atccgcccga tatagagaac ccaaaggaaa ggcgctgtcg
     7441 ccatgatggc gtagtcgaca gtggcgccgg ccagcgagac gagcaagatt ggccgccgcc
     7501 cgaaacgatc cgacagcgcg cccagcacag gtgcgcaggc aaattgcacc aacgcataca
     7561 gcgccagcag aatgccatag tgggcggtga cgtcgttcga gtgaaccaga tcgcgcagga
     7621 ggcccggcag caccggcata atcaggccga tgccgacagc gtcgagcgcg acagtgctca
     7681 gaattacgat caggggtatg ttgggtttca cgtctggcct ccggaccagc ctccgctggt
     7741 ccgattgaac gcgcggattc tttatcactg ataagttggt ggacatatta tgtttatcag
     7801 tgataaagtg tcaagcatga caaagttgca gccgaataca gtgatccgtg ccgccctgga
     7861 cctgttgaac gaggtcggcg tagacggtct gacgacacgc aaactggcgg aacggttggg
     7921 ggttcagcag ccggcgcttt actggcactt caggaacaag cgggcgctgc tcgacgcact
     7981 ggccgaagcc atgctggcgg agaatcatac gcattcggtg ccgagagccg acgacgactg
     8041 gcgctcattt ctgatcggga atgcccgcag cttcaggcag gcgctgctcg cctaccgcga
     8101 tggcgcgcgc atccatgccg gcacgcgacc gggcgcaccg cagatggaaa cggccgacgc
     8161 gcagcttcgc ttcctctgcg aggcgggttt ttcggccggg gacgccgtca atgcgctgat
     8221 gacaatcagc tacttcactg ttggggccgt gcttgaggag caggccggcg acagcgatgc
     8281 cggcgagcgc ggcggcaccg ttgaacaggc tccgctctcg ccgctgttgc gggccgcgat
     8341 agacgccttc gacgaagccg gtccggacgc agcgttcgag cagggactcg cggtgattgt
     8401 cgatggattg gcgaaaagga ggctcgttgt caggaacgtt gaaggaccga gaaagggtga
     8461 cgattgatca ggaccgctgc cggagcgcaa cccactcact acagcagagc catgtagaca
     8521 acatcccctc cccctttcca ccgcgtcaga cgcccgtagc agcccgctac gggctttttc
     8581 atgccctgcc ctagcgtcca agcctcacgg ccgcgctcgg cctctctggc ggccttctgg
     8641 cgctcctgct gcggcgtccg ctcgtgggcc gtggcgcggg tccgcgcgcc ggcctcgtgc
     8701 gcctggcgct cgcgggcgag gtccagggcg gccgtcttca cgttctgcct tgcgcagatg
     8761 agatagatcc cggaattgac ggatctagcg tggactcaag gctctcgcga atggctcgcg
     8821 ttggaaactt tcattgacac ttgaggggca ccgcagggaa attctcgtcc ttgcgagaac
     8881 cggctatgtc gtgctgcgca tcgagcctgc gcccttggct tgtctcgccc ctctccgcgt
     8941 cgctacgggg cttccagcgc ctttccgacg ctcaccgggc tggttgccct cgccgctggg
     9001 ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc cgtcgaagcc gtgtgcgaga
     9061 caccgcggcc gccggcgttg tggatacctc gcggaaaact tggccctcac tgacagatga
     9121 ggggcggacg ttgacacttg aggggccgac tcacccggcg cggcgttgac agatgagggg
     9181 caggctcgat ttcggccggc gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc
     9241 tgattttacg cgagtttccc acagatgatg tggacaagcc tggggataag tgccctgcgg
     9301 tattgacact tgaggggcgc gactactgac agatgagggg cgcgatcctt gacacttgag
     9361 gggcagagtg ctgacagatg aggggcgcac ctattgacat ttgaggggct gtccacaggc
     9421 agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta acctgtcttt
     9481 taacctgctt ttaaaccaat atttataaac cttgttttta accagggctg cgccctgtgc
     9541 gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggcccgctaa
     9601 cgcgggcctc ccatcccccc aggggctgcg cccctcggcc gcgaacggcc tcaccccaaa
     9661 aatggcagcg ctggcagtcc ttgccattgc cgggatcggg gcagtaacgg gatgggcgat
     9721 cagcccgagc gcgacgcccg gaagcattga cgtgccgcag gtgctggcat cgacattcag
     9781 cgaccaggtg ccgggcagtg agggcggcgg cctgggtggc ggcctgccct tcacttcggc
     9841 cgtcggggca ttcacggact tcatggcggg gccggcaatt tttaccttgg gcattcttgg
     9901 catagtggtc gcgggtgccg tgctcgtgtt cgggggtgaa ttaattcccc ggatcgatcc
     9961 gtcagcttca cgctgccgca agcactcagg gcgcaagggc tgctaaagga agcggaacac
    10021 gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat
    10081 ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg
    10141 gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc
    10201 gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag
    10261 gatctgatgg cgcaggggat caagatcgac ggatcgatcc ggggaattcc ggggcaatcc
    10321 cgcaaggagg gtgaatgaat cggacgtttg accggaaggc atacaggcaa gaactgatcg
    10381 acgcggggtt ttccgccgag gatgccgaaa ccatcgcaag ccgcaccgtc atgcgtgcgc
    10441 cccgcgaaac cttccagtcc gtcggctcga tggtccagca agctacggcc aagatcgagc
    10501 gcgacagcgt gcaactggct ccccctgccc tgcccgcgcc atcggccgcc gtggagcgtt
    10561 cgcgtcgtct cgaacaggag gcggcaggtt tggcgaagtc gatgaccatc gacacgcgag
    10621 gaactatgac gaccaagaag cgaaaaaccg ccggcgagga cctggcaaaa caggtcagcg
    10681 aggccaagca ggccgcgttg ctgaaacaca cgaagcagca gatcaaggaa atgcagcttt
    10741 ccttgttcga tattgcgccg tggccggaca cgatgcgagc gatgccaaac gacacggccc
    10801 gctctgccct gttcaccacg cgcaacaaga aaatcccgcg cgaggcgctg caaaacaagg
    10861 tcattttcca cgtcaacaag gacgtgaaga tcacctacac cggcgtcgag ctgcgggccg
    10921 acgatgacga actggtgtgg cagcaggtgt tggagtacgc gaagcgcacc cctatcggcg
    10981 agccgatcac cttcacgttc tacgagcttt gccaggacct gggctggtcg atcaatggcc
    11041 ggtattacac gaaggccgag gaatgcctgt cgcgcctaca ggcgacggcg atgggcttca
    11101 cgtccgaccg cgttgggcac ctggaatcgg tgtcgctgct gcaccgcttc cgcgtcctgg
    11161 accgtggcaa gaaaacgtcc cgttgccagg tcctgatcga cgaggaaatc gtcgtgctgt
    11221 ttgctggcga ccactacacg aaattcatat gggagaagta ccgcaagctg tcgccgacgg
    11281 cccgacggat gttcgactat ttcagctcgc accgggagcc gtacccgctc aagctggaaa
    11341 ccttccgcct catgtgcgga tcggattcca cccgcgtgaa gaagtggcgc gagcaggtcg
    11401 gcgaagcctg cgaagagttg cgaggcagcg gcctggtgga acacgcctgg gtcaatgatg
    11461 acctggtgca ttgcaaacgc tagggccttg tggggtcagt tccggctggg ggttcagcag
    11521 ccacctgcag gtcatcaact atcaaacgct tcggttaagc ttaaagcaca ccctttctgc
    11581 gtcctcgtat tgacgcgacg taaaatttca acgagcacgc cgggatactt accatattct
    11641 ctgctaatta tcccgacatc atcggtaaca ataaatgctg gataactggt tgctgacgca
    11701 tccatataac tcatcaaccc cggcgttcca tcaggtacag gtttcaacgt ttcaggatca
    11761 agcgctcgcg catataccca cggcggaaca tgtttacgct gcatttcatc ctcaaagaaa
    11821 caagtgttga gttcaacttg attaaatata tctcggatct gactaatatc actgagattg
    11881 aaagtatcaa ataaaagatg attgaaatca tcacgtttca gagattcttt ttcgtaactt
    11941 ttccagccgc ctccggttat gatataaagg cttttatctc cagaaaatga gattttttta
    12001 tctttcatat aatggcagag taaataaata aagtatggcg aaccaataag acaaagatct
    12061 ttcccttgat tttttattcg ttcaagacta ttcaatgttt taacaaaatc tattcgttct
    12121 tctgttacgg taaatgtcgt aggatataac aattccacca aactcataac atatttaaac
    12181 caaatattat gagcattaaa tctatctggt cccaaattga ctaattctat ttgatgatca
    12241 aaccaactac caacatattt catgccataa ctcacagagc ctaagagtct ctcaatactt
    12301 aatctgtcac gcgccacctg actttttaaa ccattcgtgc cgctactggt aaaccaactt
    12361 tcaatctcgt tttcctgaga agttaataag cgagtaaact taaaaaccga tgttgggaat
    12421 acaggtatgt catcaatttc cgtaatattg tcatctactt tgtgtgcctg acagtagtga
    12481 cgatattctc gacaatgttt ataatgatta cgaaatgcat caagcacaag tttctttctg
    12541 attttttcct gctcgtcgta agaccacact aatggatcgc tcgaaaaaat caaatcatca
    12601 atttctgagc ttgctgtaat ttcttgttta tcaacatatg aagtcatacc tgttttactc
    12661 ctcaagataa tattagaaag tatggcagca ctgctgtcat actcttttat acccttcatc
    12721 tttcaagctg ctgctttgtt ggctgctttc actcacccca gtcacatagt tatctatgct
    12781 cctggggatt cgttcacttg ccgcctcgct gcaactcgaa atctattagg tatattccat
    12841 gtggtacttc ttaatattat catcaacaat attgattaca tttttttggc tcatcaaatc
    12901 attcattggt tcaaaggaca gcaatacact tttcgcacca cacttttcaa ttgccaactt
    12961 agccgcagtt atacactccg tataatttcc gacagcgttt tctgcaatta tttcttcaag
    13021 tttattttcg aaattttcat tagggtgcat ttcaagaaca taatcactaa taaatgcacg
    13081 cgtctcttgt ttagctttat tactatcttc gttatagtta actaatatca ttaactgatg
    13141 gtctatctct gataggtcaa cgtcatattt atccgcaacg gctttatatc tttcagcata
    13201 ttcatatcta acatcattag aatcatccca cttaaagatg agaggaatac cttttttggc
    13261 cgcccactca acaatatgat gactggttgc tgttacatat ttccgaggtc cgcctggcgt
    13321 ataagcatgg ggatttacag atattttagg gaagctataa aaatcgttat ctggattaca
    13381 atagcctgtt gttaaagcat cgttaatgat ttcataacac tcttcaaata gttgctgttg
    13441 atattcaacc gggcgattaa aaaaatgcat ttcatctttt ttttcgcaat cactaaaccc
    13501 taaaataaat ctcccttcac ttaactgatc caataagcaa gcttcctccg ctatgcggac
    13561 aggatgatga gttgtaatga tgtgatttaa tgaaccaatt ttaattttct ctgttaaacc
    13621 gagcagaaaa ccagaaacag tcagaggagc gccgacaaca ccattatctg aaaaatgatt
    13681 ttcatacact aaaatctgtt caaaattcaa cttatcaaca tactccgtta tttcctgcat
    13741 gcgaactata ctttgttctt gaacagttgt tgaattgatg aagttaagga agaacaatcc
    13801 aaatttcatt tctttctcct tagctaatat aatagcgaac gttgtttttc tttaagaaat
    13861 ggcatgacat cagactggaa gagcttcatg gaagcaataa tttcgtctac tgttccatta
    13921 gcttcaaatc cacaacaaat atttgatatt cctgtagcat caatgtcttt ttgaattatg
    13981 tcaatacatt cctgcggcgt tcccacggga ttgatttcgt aactgtaatc aatacggcga
    14041 ttagtatctt tatgtccttt taatacaaag tcacgccact gccctttatt gaaatcataa
    14101 cctcttgttt ggtctgaatc atcaaaaata gtcgtagcat tcacataaga atcataccaa
    14161 tgccccagaa atttccggca aatctctttc gctttaattg agtcatgatc tacagatgtt
    14221 atatatgata agcaatggtc gatattatga atatcgtgcc catattcttg agccacttca
    14281 ttataaagct caagttgtgc tttcttttcg ttagtattta taatccaact taatatcatc
    14341 ggtaggccaa attgagcagc ccactcagtc gtcgaagctg attcagccac cacataaacc
    14401 ggtgcgccac ctctgctata cgccgcgggg tttactttta ccttatggaa cttgatatgt
    14461 tcattatcag cttccatata tccctctgtc atgccattct ttatcagccc gtaccagcat
    14521 tccgctaagg cgcgactgtt attcatatct gtgccgaata cgcgaaagtc cttgttgtaa
    14581 agccctcggc aaataccaaa ccgaaatcgt ccttttgaca tttgatccaa taaattcaca
    14641 tcttcaagtt ggcgtactgg atgggctgtg ggaagaacaa tagcggcagt tcctacattc
    14701 aattttttag tcgcgccaag taaatatgca gcagcgacat aagggttacc aagcaaacca
    14761 aactccgtga aatgatgctc cagtaaccat acggtatcaa aaccacactc ctcagagatg
    14821 cgacctaatt taaccaaacg tttcattacc tctgtttgag aaaattgggg aggttggtat
    14881 gtaagcaaaa agtttccaaa tttcatagag agtccttata ttgctatttg agtgatagaa
    14941 tatctcaata gattttaaga cagagaaatt gcttgatttt caatctcaat tctcattcgg
    15001 cgttcattga ctgtcgcaat agttaaatgt tcaaatgacg gttcagtaat atcaacatca
    15061 atatccagat gatcattatc catcgcgata gcggctttcg taaccgattg ataaaaattg
    15121 cgcaggacca ctaaattttc actcaagtca tgcgaacttc ctaacaaaga atatatcttg
    15181 catcgattac tacgaatatt tgataacaat gtgataactt catcttgctt gacccaatta
    15241 tcgttatttg cagtaaaagc aataaacggt atatcaagat acatcatgtt attaattgta
    15301 gaagctaaat cttcccaacc aaaatcaaga caatctctcg caaagacttc agcacccaat
    15361 ttatggcctt caaaatctag attattcggc aattcattaa tgggtagact gagataatca
    15421 aaccctaaag ctctttcaag agaatatctt aagttaacaa caccgactgc ggtgattaaa
    15481 aacgaagcat tgatttcaga taggcttgca taagctatcc gcgcagataa gcttgaagcc
    15541 aacataccga agttatttat ttttcgtgta gttaaccaat caaccactgc taacaagctc
    15601 tgctttccta tagacattgt aaattcatca attgtccctg aactcaatcc aacgtggtga
    15661 agcgaatcat agcggatcac atgaaatcca ttccgcgata aatattccgc cagaccagca
    15721 aaatgatcca tcctgcgggc aaaaccagac gcaataataa tggcattctt tctctttggg
    15781 ctgttttctt ctggcagcgt ttcccaaaca tgaatttttt tatttccttc aacacaaata
    15841 acgtggtcga tggttttata ttttgattca ttttccatac tttttaccta ttatgggaca
    15901 aatacaagga acttatcttc ttccaggaat cgagtctgtt ctatttcaac cgcaacatcc
    15961 ttagccgtat agttagatgg cctttcatga gaaatatatg tcactaatcg ttgcaacggt
    16021 ctcattccgt catgagatcc accaactcga aatatgttat tcattcctgc ttctacaatc
    16081 ctttccgcac cttttaatgc taacgcatct cgatatttaa atgatgactc ccaaggaaaa
    16141 atagatatgg tttgcgtctt atttttttga acataaggca atatttgctc aatattatcg
    16201 acgtgatgaa ggtacacaca tctgccaagt ggttgattaa attccacacc tgcatttgac
    16261 tcaataatca tccaacgttg atgaatatcc acctctactt ttaatccagc aaacaagctt
    16321 tctttttgaa ctaaagaata ggccgccttt tcatcaaaat cttttttggc attcggtaat
    16381 atatgcgcat atagattaag tttttctatc aacgctaact taaattcctc ataatgattt
    16441 cccatgtaat atatgttttg ggcagaaaaa caagctcgct gatcgtaaaa acaaacatca
    16501 tgagccgcac ctgtcgctgc ggacgtcaaa tcaacaggat tatcgataat gcaaagactc
    16561 tttttagaac caaatttaat cacatcagca taagatggcg catgctctac cgcccaatta
    16621 atcgcatctg gccctcccca agcgacaata acatccgcat gttgcataat ttcttttgcg
    16681 agtgatgtat caccttggtg gggccaatat ataacagata aagagcgcgt tatcggatga
    16741 ttagggtcta catcaataaa acttaacgct aatgcattag cggtaaaagg atcggttgac
    16801 gatgttttta taatacactg attcttagtt aaaattgcgc gtaatataga catgatccca
    16861 gataatggaa cattacctgc caacagatgt acagatttac ctttcggaaa agcccgaaca
    16921 taactttcat cctgaggtag ccattcatcc atgatatggc gagaaccaag ttcattttct
    16981 acaacatcat aaaggccgcc tttagaacat aaaatcatag atatccaatt ggcctctagc
    17041 ttagccattt cttctgaata tcccatatat ttttttaagt cacgaatgta tgtcctgcgt
    17101 cttgagtatt cttcattttt ccatctttgc cctaccgtat agagaaaatt gacaatgtta
    17161 tgcaaccgta attcgttatt tccattacaa tcaataatgt tttttacatg agagtcattc
    17221 aatattggca ggtaaacact attatcacca aaattaatgg attgcactaa atcatcacct
    17281 tcgggaaaga tttcaacctg gccgttaata atgaatgaaa tttttttagt catacgtatc
    17341 ctccaagcct gaattcgttt aaacg
//
