LOCUS       sequence-171189-        4503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human ABCA1 promoter luciferase reporter (P1 fragment).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Lai SC, Phelps CA, Short AM, Dutta SM, Mu D
  TITLE     Thyroid transcription factor 1 enhances cellular statin sensitivity 
            via perturbing cholesterol metabolism.
  JOURNAL   Oncogene. 2018 Mar 19. pii: 10.1038/s41388-018-0174-7. doi: 
            10.1038/s41388-018-0174-7.
  PUBMED    29551766
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2018 Mar 19. pii: 10.1038/s41388-018-0174-7. doi: 
            10.1038/s41388-018-0174-7."
COMMENT     Sequence Label: ABCA1 promoter luciferase reporter (P1 fragment)
FEATURES             Location/Qualifiers
     source          1..4503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    861..982
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(872..891)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     926..945
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1230..1247)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1401..1989)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1481..1500)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2189..2980,2981..3049))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    3154..3202
                     /note="synthetic polyadenylation signal"
     misc_feature    3216..3307
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3256..3275
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      3662..3671
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3668..4503,1..817)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 ctataagatt caatctgccc tgctggtgcc cacactattt agcttcttcg ctaagagcac
       61 tctcatcgac aagtacgacc taagcaactt gcacgagatc gccagcggcg gggcgccgct
      121 cagcaaggag gtaggtgagg ccgtggccaa acgcttccac ctaccaggca tccgccaggg
      181 ctacggcctg acagaaacaa ccagcgccat tctgatcacc cccgaagggg acgacaagcc
      241 tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag gtggtggact tggacaccgg
      301 taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca tgatcatgag
      361 cggctacgtt aacaaccccg aggctacaaa cgctctcatc gacaaggacg gctggctgca
      421 cagcggcgac atcgcctact gggacgagga cgagcacttc ttcatcgtgg accggctgaa
      481 gagcctgatc aaatacaagg gctaccaggt agccccagcc gaactggaga gcatcctgct
      541 gcaacacccc aacatcttcg acgccggggt cgccggcctg cccgacgacg atgccggcga
      601 gctgcccgcc gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga aggagatcgt
      661 ggactatgtg gccagccagg ttacaaccgc caagaagctg cgcggtggtg ttgtgttcgt
      721 ggacgaggtg cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc gcgagattct
      781 cattaaggcc aagaagggcg gcaagatcgc cgtgtaataa ttctagagtc ggggcggccg
      841 gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat
      901 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
      961 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     1021 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga
     1081 taaggatccg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     1141 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
     1201 gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1261 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1321 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1381 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1441 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1501 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1561 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1621 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1681 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1741 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1801 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1861 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1921 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1981 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     2041 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     2101 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc
     2161 ggccgcaaat gctaaaccac tgcagtggtt accagtgctt gatcagtgag gcaccgatct
     2221 cagcgatctg cctatttcgt tcgtccatag tggcctgact ccccgtcgtg tagatcacta
     2281 cgattcgtga gggcttacca tcaggcccca gcgcagcaat gatgccgcga gagccgcgtt
     2341 caccggcccc cgatttgtca gcaatgaacc agccagcagg gagggccgag cgaagaagtg
     2401 gtcctgctac tttgtccgcc tccatccagt ctatgagctg ctgtcgtgat gctagagtaa
     2461 gaagttcgcc agtgagtagt ttccgaagag ttgtggccat tgctactggc atcgtggtat
     2521 cacgctcgtc gttcggtatg gcttcgttca actctggttc ccagcggtca agccgggtca
     2581 catgatcacc catattatga agaaatgcag tcagctcctt agggcctccg atcgttgtca
     2641 gaagtaagtt ggccgcggtg ttgtcgctca tggtaatggc agcactacac aattctctta
     2701 ccgtcatgcc atccgtaaga tgcttttccg tgaccggcga gtactcaacc aagtcgtttt
     2761 gtgagtagtg tatacggcga ccaagctgct cttgcccggc gtctatacgg gacaacaccg
     2821 cgccacatag cagtactttg aaagtgctca tcatcgggaa tcgttcttcg gggcggaaag
     2881 actcaaggat cttgccgcta ttgagatcca gttcgatata gcccactctt gcacccagtt
     2941 gatcttcagc atcttttact ttcaccagcg tttcggggtg tgcaaaaaca ggcaagcaaa
     3001 atgccgcaaa gaagggaatg agtgcgacac gaaaatgttg gatgctcata ctcgtccttt
     3061 ttcaatatta ttgaagcatt tatcagggtt actagtacgt ctctcaagga taagtaagta
     3121 atattaaggt acgggaggta ttggacaggc cgcaataaaa tatctttatt ttcattacat
     3181 ctgtgtgttg gttttttgtg tgaatcgata gtactaacat acgctctcca tcaaaacaaa
     3241 acgaaacaaa acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca
     3301 tttctctggc ctaactggcc ggtacctgag ctcgctagca tgttcctctc gggtcctctg
     3361 agggacctgg ggagctcagg ctgggaatct ccaaggcagt aggtcgccta tcaaaaatca
     3421 aagtccaggt ttgtgggggg aaaacaaaag cagcccatta cccagaggac tgtccgcctt
     3481 cccctcaccc cagcctaggc ctttgaaagg aaacaaaaga caagacaaaa tgattggcgt
     3541 cctgagggag attcagccta gagctctctc tcccccaatc cctccctccg gctgaggaaa
     3601 ctaacaaagg aaaagatctg gcctcggcgg ccaagcttgg caatccggta ctgttggtaa
     3661 agccaccatg gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga
     3721 agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg
     3781 caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga
     3841 gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat
     3901 cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat
     3961 cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat
     4021 gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa
     4081 cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta
     4141 ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga
     4201 gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag
     4261 tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg
     4321 attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct
     4381 cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg
     4441 cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca
     4501 aga
//
