LOCUS       sequence-171190-        6248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  VSV-G-expressing envelope plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Ou W, Marino MP, Suzuki A, Joshi B, Husain SR, Maisner A, Galanis E,
            Puri RK, Reiser J
  TITLE     Specific targeting of human interleukin (IL)-13 receptor 
            alpha2-positive cells with lentiviral vectors displaying IL-13.
  JOURNAL   Hum Gene Ther Methods. 2012 Apr;23(2):137-47. Epub 2012 May 21.
  PUBMED    22612657
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther Methods."; date: "2012-04"; volume: "23(2)"; pages: "137-47. 
            Epub 2012 May 21"
COMMENT     Sequence Label: pCEF-VSV-G
FEATURES             Location/Qualifiers
     source          1..6248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..456
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        466..1648
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          697..1639
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1596..1616
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(1679..1726,1727..3214)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     primer_bind     complement(3370..3389)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3435..3490
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3489..3508)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3583..3600)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3754..4342)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3834..3853)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4884..5699)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4884..5699)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5607..5626
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5691..5710
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     5977..5995
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6033..6055)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6155..6174
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tggccattgc atacgttgta tccatatcat aatatgtaca tttatattgg ctcatgtcca
       61 acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
      121 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      181 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      241 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      301 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      361 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      421 cagtacatct acgtattagt catcgctatt acccggcatg cgtgaggctc cggtgcccgt
      481 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
      541 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
      601 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
      661 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
      721 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacgcccctg
      781 gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag
      841 gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg
      901 gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc
      961 tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct
     1021 tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga
     1081 cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc
     1141 gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc
     1201 gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc
     1261 ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc
     1321 gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt
     1381 cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag
     1441 cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca
     1501 cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga
     1561 atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag
     1621 tttttttctt ccatttcagg tgtcgtgagg aattctgcag tcgaggaatt ctgacactat
     1681 gaagtgcctt ttgtacttag cctttttatt cattggggtg aattgcaagt tcaccatagt
     1741 ttttccacac aaccaaaaag gaaactggaa aaatgttcct tctaattacc attattgccc
     1801 gtcaagctca gatttaaatt ggcataatga cttaataggc acagccttac aagtcaaaat
     1861 gcccaagagt cacaaggcta ttcaagcaga cggttggatg tgtcatgctt ccaaatgggt
     1921 cactacttgt gatttccgct ggtatggacc gaagtatata acacattcca tccgatcctt
     1981 cactccatct gtagaacaat gcaaggaaag cattgaacaa acgaaacaag gaacttggct
     2041 gaatccaggc ttccctcctc aaagttgtgg atatgcaact gtgacggatg ccgaagcagt
     2101 gattgtccag gtgactcctc accatgtgct ggttgatgaa tacacaggag aatgggttga
     2161 ttcacagttc atcaacggaa aatgcagcaa ttacatatgc cccactgtcc ataactctac
     2221 aacctggcat tctgactata aggtcaaagg gctatgtgat tctaacctca tttccatgga
     2281 catcaccttc ttctcagagg acggagagct atcatccctg ggaaaggagg gcacagggtt
     2341 cagaagtaac tactttgctt atgaaactgg aggcaaggcc tgcaaaatgc aatactgcaa
     2401 gcattgggga gtcagactcc catcaggtgt ctggttcgag atggctgata aggatctctt
     2461 tgctgcagcc agattccctg aatgcccaga agggtcaagt atctctgctc catctcagac
     2521 ctcagtggat gtaagtctaa ttcaggacgt tgagaggatc ttggattatt ccctctgcca
     2581 agaaacctgg agcaaaatca gagcgggtct tccaatctct ccagtggatc tcagctatct
     2641 tgctcctaaa aacccaggaa ccggtcctgc tttcaccata atcaatggta ccctaaaata
     2701 ctttgagacc agatacatca gagtcgatat tgctgctcca atcctctcaa gaatggtcgg
     2761 aatgatcagt ggaactacca cagaaaggga actgtgggat gactgggcac catatgaaga
     2821 cgtggaaatt ggacccaatg gagttctgag gaccagttca ggatataagt ttcctttata
     2881 catgattgga catggtatgt tggactccga tcttcatctt agctcaaagg ctcaggtgtt
     2941 cgaacatcct cacattcaag acgctgcttc gcaacttcct gatgatgaga gtttattttt
     3001 tggtgatact gggctatcca aaaatccaat cgagcttgta gaaggttggt tcagtagttg
     3061 gaaaagctct attgcctctt ttttctttat catagggtta atcattggac tattcttggt
     3121 tctccgagtt ggtatccatc tttgcattaa attaaagcac accaagaaaa gacagattta
     3181 tacagacata gagatgaacc gacttggaaa gtaactcaaa tcctgcacaa cagattcttc
     3241 atgtttggac caaatcaact tgtgatacca tgctcaaaga ggcctcaatt atatttgagt
     3301 ttttaatttt tatgaaaaaa aaaaaaaaaa acggaattcc tcgacggtac cgcgggcccg
     3361 ggatccagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     3421 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     3481 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     3541 tttggtttag agtttggcaa catatgccca ttcttccgct tcctcgctca ctgactcgct
     3601 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     3661 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     3721 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     3781 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3841 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     3901 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3961 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4021 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4081 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4141 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4201 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4261 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4321 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4381 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4441 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     4501 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     4561 tcgttcatcc atagttgcct gactcggggg gggggggcgc tgaggtctgc ctcgtgaaga
     4621 aggtgttgct gactcatacc aggcctgaat cgccccatca tccagccaga aagtgaggga
     4681 gccacggttg atgagagctt tgttgtaggt ggaccagttg gtgattttga acttttgctt
     4741 tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca actcagcaaa
     4801 agttcgattt attcaacaaa gccgccgtcc cgtcaagtca gcgtaatgct ctgccagtgt
     4861 tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat
     4921 ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga
     4981 gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg
     5041 actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt
     5101 gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct
     5161 ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc
     5221 aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa
     5281 ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca
     5341 atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc
     5401 gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga
     5461 ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg
     5521 ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag
     5581 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca
     5641 tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat atggctcata
     5701 acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt
     5761 ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttc cccccccccc
     5821 cattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5881 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     5941 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     6001 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     6061 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     6121 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     6181 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcaga
     6241 ttggctat
//
