LOCUS       sequence-171191-        4577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human ABCA1 promoter luciferase reporter (P3 fragment).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Lai SC, Phelps CA, Short AM, Dutta SM, Mu D
  TITLE     Thyroid transcription factor 1 enhances cellular statin sensitivity 
            via perturbing cholesterol metabolism.
  JOURNAL   Oncogene. 2018 Mar 19. pii: 10.1038/s41388-018-0174-7. doi: 
            10.1038/s41388-018-0174-7.
  PUBMED    29551766
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2018 Mar 19. pii: 10.1038/s41388-018-0174-7. doi: 
            10.1038/s41388-018-0174-7."
COMMENT     Sequence Label: ABCA1 promoter luciferase reporter (P3 fragment)
FEATURES             Location/Qualifiers
     source          1..4577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    744..865
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(755..774)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     809..828
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1113..1130)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1284..1872)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1364..1383)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2072..2863,2864..2932))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    3037..3085
                     /note="synthetic polyadenylation signal"
     misc_feature    3099..3190
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3139..3158
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      3619..3628
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3625..4577,1..700)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca
       61 gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa
      121 gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac
      181 cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat
      241 gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct
      301 gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct
      361 gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct
      421 gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg
      481 cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat
      541 cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt
      601 cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat
      661 tctcattaag gccaagaagg gcggcaagat cgccgtgtaa taattctaga gtcggggcgg
      721 ccggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag
      781 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
      841 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
      901 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat
      961 cgataaggat ccgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     1021 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     1081 taggacaggt gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1141 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1201 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1261 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1321 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1381 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1441 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1501 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1561 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1621 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1681 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1741 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1801 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1861 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1921 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1981 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2041 agcggccgca aatgctaaac cactgcagtg gttaccagtg cttgatcagt gaggcaccga
     2101 tctcagcgat ctgcctattt cgttcgtcca tagtggcctg actccccgtc gtgtagatca
     2161 ctacgattcg tgagggctta ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc
     2221 gttcaccggc ccccgatttg tcagcaatga accagccagc agggagggcc gagcgaagaa
     2281 gtggtcctgc tactttgtcc gcctccatcc agtctatgag ctgctgtcgt gatgctagag
     2341 taagaagttc gccagtgagt agtttccgaa gagttgtggc cattgctact ggcatcgtgg
     2401 tatcacgctc gtcgttcggt atggcttcgt tcaactctgg ttcccagcgg tcaagccggg
     2461 tcacatgatc acccatatta tgaagaaatg cagtcagctc cttagggcct ccgatcgttg
     2521 tcagaagtaa gttggccgcg gtgttgtcgc tcatggtaat ggcagcacta cacaattctc
     2581 ttaccgtcat gccatccgta agatgctttt ccgtgaccgg cgagtactca accaagtcgt
     2641 tttgtgagta gtgtatacgg cgaccaagct gctcttgccc ggcgtctata cgggacaaca
     2701 ccgcgccaca tagcagtact ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga
     2761 aagactcaag gatcttgccg ctattgagat ccagttcgat atagcccact cttgcaccca
     2821 gttgatcttc agcatctttt actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc
     2881 aaaatgccgc aaagaaggga atgagtgcga cacgaaaatg ttggatgctc atactcgtcc
     2941 tttttcaata ttattgaagc atttatcagg gttactagta cgtctctcaa ggataagtaa
     3001 gtaatattaa ggtacgggag gtattggaca ggccgcaata aaatatcttt attttcatta
     3061 catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac
     3121 aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga
     3181 acatttctct ggcctaactg gccggtacct gagctcgcta gcttgaccga tagtaacctc
     3241 tgcgctcggt gcagccgaat ctataaaagg aactagtccc ggcaaaaacc ccgtaattgc
     3301 gagcgagagt gagtggggcc gggacccgca gagccgagcc gacccttctc tcccgggctg
     3361 cggcagggca gggcggggag ctccgcgcac caacagagcc ggttctcagg gcgctttgct
     3421 ccttgttttt tccccggttc tgttttctcc ccttctccgg aaggcttgtc aaggggtagg
     3481 agaaagagac gcaaacacaa aagtggaaaa caggtaagag gctctccagt gacttacttg
     3541 ggcgttattg ttttgtttcg aggccaagga agatctggcc tcggcggcca agcttggcaa
     3601 tccggtactg ttggtaaagc caccatggaa gatgccaaaa acattaagaa gggcccagcg
     3661 ccattctacc cactcgaaga cgggaccgcc ggcgagcagc tgcacaaagc catgaagcgc
     3721 tacgccctgg tgcccggcac catcgccttt accgacgcac atatcgaggt ggacattacc
     3781 tacgccgagt acttcgagat gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg
     3841 aatacaaacc atcggatcgt ggtgtgcagc gagaatagct tgcagttctt catgcccgtg
     3901 ttgggtgccc tgttcatcgg tgtggctgtg gccccagcta acgacatcta caacgagcgc
     3961 gagctgctga acagcatggg catcagccag cccaccgtcg tattcgtgag caagaaaggg
     4021 ctgcaaaaga tcctcaacgt gcaaaagaag ctaccgatca tacaaaagat catcatcatg
     4081 gatagcaaga ccgactacca gggcttccaa agcatgtaca ccttcgtgac ttcccatttg
     4141 ccacccggct tcaacgagta cgacttcgtg cccgagagct tcgaccggga caaaaccatc
     4201 gccctgatca tgaacagtag tggcagtacc ggattgccca agggcgtagc cctaccgcac
     4261 cgcaccgctt gtgtccgatt cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc
     4321 cccgacaccg ctatcctcag cgtggtgcca tttcaccacg gcttcggcat gttcaccacg
     4381 ctgggctact tgatctgcgg ctttcgggtc gtgctcatgt accgcttcga ggaggagcta
     4441 ttcttgcgca gcttgcaaga ctataagatt caatctgccc tgctggtgcc cacactattt
     4501 agcttcttcg ctaagagcac tctcatcgac aagtacgacc taagcaactt gcacgagatc
     4561 gccagcggcg gggcgcc
//
