LOCUS       sequence-171197-        4623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human ABCA1 promoter luciferase reporter (P2 fragment).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Lai SC, Phelps CA, Short AM, Dutta SM, Mu D
  TITLE     Thyroid transcription factor 1 enhances cellular statin sensitivity 
            via perturbing cholesterol metabolism.
  JOURNAL   Oncogene. 2018 Mar 19. pii: 10.1038/s41388-018-0174-7. doi: 
            10.1038/s41388-018-0174-7.
  PUBMED    29551766
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2018 Mar 19. pii: 10.1038/s41388-018-0174-7. doi: 
            10.1038/s41388-018-0174-7."
COMMENT     Sequence Label: ABCA1 promoter luciferase reporter (P2 fragment)
FEATURES             Location/Qualifiers
     source          1..4623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1640..1761
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1651..1670)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1705..1724
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2009..2026)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2180..2768)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2260..2279)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2968..3759,3760..3828))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    3933..3981
                     /note="synthetic polyadenylation signal"
     misc_feature    3995..4086
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4035..4054
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      4561..4570
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4567..4623,1..1596)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 gggaccgccg gcgagcagct gcacaaagcc atgaagcgct acgccctggt gcccggcacc
       61 atcgccttta ccgacgcaca tatcgaggtg gacattacct acgccgagta cttcgagatg
      121 agcgttcggc tggcagaagc tatgaagcgc tatgggctga atacaaacca tcggatcgtg
      181 gtgtgcagcg agaatagctt gcagttcttc atgcccgtgt tgggtgccct gttcatcggt
      241 gtggctgtgg ccccagctaa cgacatctac aacgagcgcg agctgctgaa cagcatgggc
      301 atcagccagc ccaccgtcgt attcgtgagc aagaaagggc tgcaaaagat cctcaacgtg
      361 caaaagaagc taccgatcat acaaaagatc atcatcatgg atagcaagac cgactaccag
      421 ggcttccaaa gcatgtacac cttcgtgact tcccatttgc cacccggctt caacgagtac
      481 gacttcgtgc ccgagagctt cgaccgggac aaaaccatcg ccctgatcat gaacagtagt
      541 ggcagtaccg gattgcccaa gggcgtagcc ctaccgcacc gcaccgcttg tgtccgattc
      601 agtcatgccc gcgaccccat cttcggcaac cagatcatcc ccgacaccgc tatcctcagc
      661 gtggtgccat ttcaccacgg cttcggcatg ttcaccacgc tgggctactt gatctgcggc
      721 tttcgggtcg tgctcatgta ccgcttcgag gaggagctat tcttgcgcag cttgcaagac
      781 tataagattc aatctgccct gctggtgccc acactattta gcttcttcgc taagagcact
      841 ctcatcgaca agtacgacct aagcaacttg cacgagatcg ccagcggcgg ggcgccgctc
      901 agcaaggagg taggtgaggc cgtggccaaa cgcttccacc taccaggcat ccgccagggc
      961 tacggcctga cagaaacaac cagcgccatt ctgatcaccc ccgaagggga cgacaagcct
     1021 ggcgcagtag gcaaggtggt gcccttcttc gaggctaagg tggtggactt ggacaccggt
     1081 aagacactgg gtgtgaacca gcgcggcgag ctgtgcgtcc gtggccccat gatcatgagc
     1141 ggctacgtta acaaccccga ggctacaaac gctctcatcg acaaggacgg ctggctgcac
     1201 agcggcgaca tcgcctactg ggacgaggac gagcacttct tcatcgtgga ccggctgaag
     1261 agcctgatca aatacaaggg ctaccaggta gccccagccg aactggagag catcctgctg
     1321 caacacccca acatcttcga cgccggggtc gccggcctgc ccgacgacga tgccggcgag
     1381 ctgcccgccg cagtcgtcgt gctggaacac ggtaaaacca tgaccgagaa ggagatcgtg
     1441 gactatgtgg ccagccaggt tacaaccgcc aagaagctgc gcggtggtgt tgtgttcgtg
     1501 gacgaggtgc ctaaaggact gaccggcaag ttggacgccc gcaagatccg cgagattctc
     1561 attaaggcca agaagggcgg caagatcgcc gtgtaataat tctagagtcg gggcggccgg
     1621 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1681 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1741 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     1801 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat
     1861 aaggatccgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     1921 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     1981 acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2041 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2101 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2161 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2221 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2281 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2341 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2401 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2461 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2521 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2581 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2641 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2701 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2761 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2821 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2881 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagcg
     2941 gccgcaaatg ctaaaccact gcagtggtta ccagtgcttg atcagtgagg caccgatctc
     3001 agcgatctgc ctatttcgtt cgtccatagt ggcctgactc cccgtcgtgt agatcactac
     3061 gattcgtgag ggcttaccat caggccccag cgcagcaatg atgccgcgag agccgcgttc
     3121 accggccccc gatttgtcag caatgaacca gccagcaggg agggccgagc gaagaagtgg
     3181 tcctgctact ttgtccgcct ccatccagtc tatgagctgc tgtcgtgatg ctagagtaag
     3241 aagttcgcca gtgagtagtt tccgaagagt tgtggccatt gctactggca tcgtggtatc
     3301 acgctcgtcg ttcggtatgg cttcgttcaa ctctggttcc cagcggtcaa gccgggtcac
     3361 atgatcaccc atattatgaa gaaatgcagt cagctcctta gggcctccga tcgttgtcag
     3421 aagtaagttg gccgcggtgt tgtcgctcat ggtaatggca gcactacaca attctcttac
     3481 cgtcatgcca tccgtaagat gcttttccgt gaccggcgag tactcaacca agtcgttttg
     3541 tgagtagtgt atacggcgac caagctgctc ttgcccggcg tctatacggg acaacaccgc
     3601 gccacatagc agtactttga aagtgctcat catcgggaat cgttcttcgg ggcggaaaga
     3661 ctcaaggatc ttgccgctat tgagatccag ttcgatatag cccactcttg cacccagttg
     3721 atcttcagca tcttttactt tcaccagcgt ttcggggtgt gcaaaaacag gcaagcaaaa
     3781 tgccgcaaag aagggaatga gtgcgacacg aaaatgttgg atgctcatac tcgtcctttt
     3841 tcaatattat tgaagcattt atcagggtta ctagtacgtc tctcaaggat aagtaagtaa
     3901 tattaaggta cgggaggtat tggacaggcc gcaataaaat atctttattt tcattacatc
     3961 tgtgtgttgg ttttttgtgt gaatcgatag tactaacata cgctctccat caaaacaaaa
     4021 cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat
     4081 ttctctggcc taactggccg gtacctgagc tcgctagcgc tgaggaaact aacaaaggaa
     4141 aaaaaaattg cggaaagcag gatttagagg aagcaaattc cactggtgcc cttggctgcc
     4201 gggaacgtgg actagagagt ctgcggcgca gccccgagcc cagcgcttcc cgcgcgtctt
     4261 aggccggcgg gcccgggcgg gggaagggga cgcagaccgc ggaccctaag acacctgctg
     4321 taccctccac ccccacccca cccacctccc cccaactccc tagatgtgtc gtgggcggct
     4381 gaacgtcgcc cgtttaaggg gcgggccccg gctccacgtg ctttctgctg agtgactgaa
     4441 ctacataaac agaggccggg aacggggcgg ggaggaggga gagcacaggc tttgaccgat
     4501 agtaacctct gcagatctgg cctcggcggc caagcttggc aatccggtac tgttggtaaa
     4561 gccaccatgg aagatgccaa aaacattaag aagggcccag cgccattcta cccactcgaa
     4621 gac
//
