LOCUS       sequence-171199-        5202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human ABCA1 promoter luciferase reporter (Full-length).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Lai SC, Phelps CA, Short AM, Dutta SM, Mu D
  TITLE     Thyroid transcription factor 1 enhances cellular statin sensitivity 
            via perturbing cholesterol metabolism.
  JOURNAL   Oncogene. 2018 Mar 19. pii: 10.1038/s41388-018-0174-7. doi: 
            10.1038/s41388-018-0174-7.
  PUBMED    29551766
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2018 Mar 19. pii: 10.1038/s41388-018-0174-7. doi: 
            10.1038/s41388-018-0174-7."
COMMENT     Sequence Label: ABCA1 promoter luciferase reporter (Full-length)
FEATURES             Location/Qualifiers
     source          1..5202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      237..246
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             243..1895
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    1939..2060
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1950..1969)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2004..2023
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2308..2325)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2479..3067)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2559..2578)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3267..4058,4059..4127))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4232..4280
                     /note="synthetic polyadenylation signal"
     misc_feature    4294..4385
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4334..4353
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 agccggttct cagggcgctt tgctccttgt tttttccccg gttctgtttt ctccccttct
       61 ccggaaggct tgtcaagggg taggagaaag agacgcaaac acaaaagtgg aaaacaggta
      121 agaggctctc cagtgactta cttgggcgtt attgttttgt ttcgaggcca aggaggcttc
      181 gggaagtgag atctggcctc ggcggccaag cttggcaatc cggtactgtt ggtaaagcca
      241 ccatggaaga tgccaaaaac attaagaagg gcccagcgcc attctaccca ctcgaagacg
      301 ggaccgccgg cgagcagctg cacaaagcca tgaagcgcta cgccctggtg cccggcacca
      361 tcgcctttac cgacgcacat atcgaggtgg acattaccta cgccgagtac ttcgagatga
      421 gcgttcggct ggcagaagct atgaagcgct atgggctgaa tacaaaccat cggatcgtgg
      481 tgtgcagcga gaatagcttg cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg
      541 tggctgtggc cccagctaac gacatctaca acgagcgcga gctgctgaac agcatgggca
      601 tcagccagcc caccgtcgta ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc
      661 aaaagaagct accgatcata caaaagatca tcatcatgga tagcaagacc gactaccagg
      721 gcttccaaag catgtacacc ttcgtgactt cccatttgcc acccggcttc aacgagtacg
      781 acttcgtgcc cgagagcttc gaccgggaca aaaccatcgc cctgatcatg aacagtagtg
      841 gcagtaccgg attgcccaag ggcgtagccc taccgcaccg caccgcttgt gtccgattca
      901 gtcatgcccg cgaccccatc ttcggcaacc agatcatccc cgacaccgct atcctcagcg
      961 tggtgccatt tcaccacggc ttcggcatgt tcaccacgct gggctacttg atctgcggct
     1021 ttcgggtcgt gctcatgtac cgcttcgagg aggagctatt cttgcgcagc ttgcaagact
     1081 ataagattca atctgccctg ctggtgccca cactatttag cttcttcgct aagagcactc
     1141 tcatcgacaa gtacgaccta agcaacttgc acgagatcgc cagcggcggg gcgccgctca
     1201 gcaaggaggt aggtgaggcc gtggccaaac gcttccacct accaggcatc cgccagggct
     1261 acggcctgac agaaacaacc agcgccattc tgatcacccc cgaaggggac gacaagcctg
     1321 gcgcagtagg caaggtggtg cccttcttcg aggctaaggt ggtggacttg gacaccggta
     1381 agacactggg tgtgaaccag cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg
     1441 gctacgttaa caaccccgag gctacaaacg ctctcatcga caaggacggc tggctgcaca
     1501 gcggcgacat cgcctactgg gacgaggacg agcacttctt catcgtggac cggctgaaga
     1561 gcctgatcaa atacaagggc taccaggtag ccccagccga actggagagc atcctgctgc
     1621 aacaccccaa catcttcgac gccggggtcg ccggcctgcc cgacgacgat gccggcgagc
     1681 tgcccgccgc agtcgtcgtg ctggaacacg gtaaaaccat gaccgagaag gagatcgtgg
     1741 actatgtggc cagccaggtt acaaccgcca agaagctgcg cggtggtgtt gtgttcgtgg
     1801 acgaggtgcc taaaggactg accggcaagt tggacgcccg caagatccgc gagattctca
     1861 ttaaggccaa gaagggcggc aagatcgccg tgtaataatt ctagagtcgg ggcggccggc
     1921 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     1981 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     2041 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     2101 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata
     2161 aggatccgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
     2221 cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga
     2281 caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     2341 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2401 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2461 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2521 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2581 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2641 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2701 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2761 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2821 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2881 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2941 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3001 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3061 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3121 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3181 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagcgg
     3241 ccgcaaatgc taaaccactg cagtggttac cagtgcttga tcagtgaggc accgatctca
     3301 gcgatctgcc tatttcgttc gtccatagtg gcctgactcc ccgtcgtgta gatcactacg
     3361 attcgtgagg gcttaccatc aggccccagc gcagcaatga tgccgcgaga gccgcgttca
     3421 ccggcccccg atttgtcagc aatgaaccag ccagcaggga gggccgagcg aagaagtggt
     3481 cctgctactt tgtccgcctc catccagtct atgagctgct gtcgtgatgc tagagtaaga
     3541 agttcgccag tgagtagttt ccgaagagtt gtggccattg ctactggcat cgtggtatca
     3601 cgctcgtcgt tcggtatggc ttcgttcaac tctggttccc agcggtcaag ccgggtcaca
     3661 tgatcaccca tattatgaag aaatgcagtc agctccttag ggcctccgat cgttgtcaga
     3721 agtaagttgg ccgcggtgtt gtcgctcatg gtaatggcag cactacacaa ttctcttacc
     3781 gtcatgccat ccgtaagatg cttttccgtg accggcgagt actcaaccaa gtcgttttgt
     3841 gagtagtgta tacggcgacc aagctgctct tgcccggcgt ctatacggga caacaccgcg
     3901 ccacatagca gtactttgaa agtgctcatc atcgggaatc gttcttcggg gcggaaagac
     3961 tcaaggatct tgccgctatt gagatccagt tcgatatagc ccactcttgc acccagttga
     4021 tcttcagcat cttttacttt caccagcgtt tcggggtgtg caaaaacagg caagcaaaat
     4081 gccgcaaaga agggaatgag tgcgacacga aaatgttgga tgctcatact cgtccttttt
     4141 caatattatt gaagcattta tcagggttac tagtacgtct ctcaaggata agtaagtaat
     4201 attaaggtac gggaggtatt ggacaggccg caataaaata tctttatttt cattacatct
     4261 gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc aaaacaaaac
     4321 gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg ccagaacatt
     4381 tctctggcct aactggccgg taccatgttc ctctcgggtc ctctgaggga cctggggagc
     4441 tcaggctggg aatctccaag gcagtaggtc gcctatcaaa aatcaaagtc caggtttgtg
     4501 gggggaaaac aaaagcagcc cattacccag aggactgtcc gccttcccct caccccagcc
     4561 taggcctttg aaaggaaaca aaagacaaga caaaatgatt ggcgtcctga gggagattca
     4621 gcctagagct ctctctcccc caatccctcc ctccggctga ggaaactaac aaaggaaaaa
     4681 aaaattgcgg aaagcaggat ttagaggaag caaattccac tggtgccctt ggctgccggg
     4741 aacgtggact agagagtctg cggcgcagcc ccgagcccag cgcttcccgc gcgtcttagg
     4801 ccggcgggcc cgggcggggg aaggggacgc agaccgcgga ccctaagaca cctgctgtac
     4861 cctccacccc caccccaccc acctcccccc aactccctag atgtgtcgtg ggcggctgaa
     4921 cgtcgcccgt ttaaggggcg ggccccggct ccacgtgctt tctgctgagt gactgaacta
     4981 cataaacaga ggccgggaac ggggcgggga ggagggagag cacaggcttt gaccgatagt
     5041 aacctctgcg ctcggtgcag ccgaatctat aaaaggaact agtcccggca aaaaccccgt
     5101 aattgcgagc gagagtgagt ggggccggga cccgcagagc cgagccgacc cttctctccc
     5161 gggctgcggc agggcagggc ggggagctcc gcgcaccaac ag
//
