LOCUS       sequence-171204-       17503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Malonate biosensor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17503)
  AUTHORS   Pini F, East AK, Appia-Ayme C, Tomek J, Karunakaran R, 
            Mendoza-Suarez M, Edwards A, Terpolilli JJ, Roworth J, Downie JA, 
            Poole PS
  TITLE     Bacterial Biosensors for in Vivo Spatiotemporal Mapping of Root 
            Secretion.
  JOURNAL   Plant Physiol. 2017 Jul;174(3):1289-1306. doi: 10.1104/pp.16.01302. 
            Epub 2017 May 11.
  PUBMED    28495892
REFERENCE   2  (bases 1 to 17503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.16.01302"; journalName: "Plant Physiol"; date: 
            "2017-07"; volume: "174"; issue: "3"; pages: "1289-1306"
COMMENT     Sequence Label: pLMB737
FEATURES             Location/Qualifiers
     source          1..17503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4540..4556)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4540..4556)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4553..4575)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4564..4580
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4588..4594,4595..4612,4613..4618))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4633..4654
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4779..4797)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     oriT            4842..4950
                     /note="incP origin of transfer"
     promoter        5127..5231
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5232..5300,5301..6092)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5450..5469)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(6619..7818)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             7924..8574
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     rep_origin      9064..9775
                     /label=oriV
                     /note="incP origin of replication"
     CDS             10442..11590
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11679..12785)
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFD"
     CDS             complement(12964..13947)
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             complement(13962..15044)
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             complement(15093..16016)
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             complement(16029..17471)
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
ORIGIN
        1 gatccatcgc ctggtcgagc ttggccgtgt cggggccgaa cttttcatgc agcatatcga
       61 ggaatgcctg cgcgccctcg ctctcgagcg cgccccagcg atcgagtatc tcggcagcaa
      121 gcgccatgcc cgaggcttcg ccccggctcg acagcagcgt ttcacagagt gtctggagat
      181 cggccttggc tgcgacctgc gtggcgcgcg agccggaaaa cagaagctgg cgtccgcgat
      241 ccgtgatgct ctgcagcatg tcggtgaaga aggaagcctc agacatgcca ttctcctctc
      301 ttctgctggt attttctgtt gggtcggctg ggtgcggacc gtttttgacg gctgcttccc
      361 atcgtcgggt ttcaccggta gtatgtagaa tgccactctt cgtacacgag gtcaatgatc
      421 gtgtatacaa gaagtgcttt tatgtatgag ggaattgaaa cgaatgtccg agaatgtcgg
      481 ccgatggctt cgcgatgaga ttgaaaattc aattctttcc aacgagttct cccccggtga
      541 gcggctcgac gaaacagtgc ttgcgacgcg tttcggggtc tcccgcacgc ctgtccgtga
      601 ggcgctgatg cagctcgatg cgatcggcct catcgaaatt cgaccgcgca gaggcgcaat
      661 cgtcatcgat ccggggccgc accgcgtcta cgagatgttc gaggtgatgg ccgaattgga
      721 gggcctggcc gggtcgctcg ccgcacgacg gctggacaag gcctcccggg aagcgatcac
      781 ggccacccac agccgctgcg ggcaatcagc ctctgccggt gacagcgatg cctattatta
      841 cgacaatgag gaattccata aggccatcta tgcggccggc cgaagcgaat tcctcgagga
      901 gcaatgtgtg cagttgcacc ggcgcctgcg gccttatcgc cgcctccagc tgcgcgtgcg
      961 caaccgtctg tcgacgtcct tctcagaaca ttgcgccatc gtcgacgcga tctttgccgg
     1021 agacggagat gaagcccgcc gcctgctgcg gacgcatgtc ggcattcagg gagagaggtt
     1081 cagcgatctg gtcgcaagca tggcggtcag atgattggac tgctggagta atctcaagcg
     1141 gcaggaaagc cccacacccg cgcgatggaa ctcgtcatcc gatgcgcgcg ggtctccgtc
     1201 aggaaaaatt ctccagcacg gccaggaatg tgtgccggtg gtacccccac cgcggactct
     1261 gactacaacc gtgcgcaagg cgcattaggg aggatgtatg tatgtaatcg cctgcggcat
     1321 cgttgccggc ttggcggctg cggtggccct gttgggcttc acgccgatga tggaggcgct
     1381 tgccgccggc gaacgccgca aggcactcgc gcaatggacg cggacgatgt tcctggtgct
     1441 gctgcctgtc gtgctgatgt gcgcgcccat cgggtccagc atttacgacg ccgtgcaagc
     1501 ggacgctggc aagcccatcg ctttccacaa cggccggatc acggtcgtca tggccctggt
     1561 cggcagcttg gccgttgtcc tggtcgcggc tgcgcgtgcg gtggtcaacc gcaagcatgc
     1621 cagcttctgg ttcgtcggct gggtgatggc gtcggttttg gccggcggcg tcggcgcgat
     1681 cgccagcgcg aagcaactgg cgttcctcgg cgaacatagc ggcatggtgg ccttcggctt
     1741 cttccgcgac caggtgaagg acatgcactg cgatgcggac gtgatcctgg cccggtggga
     1801 tgaaaaggcg aactcgccgg tggtctaccg ctgcccgaag gcgtacctgc tcaacaggtt
     1861 cgcatccgcg cccttcgtgc cctggccgga ctacaccgag ggggaaagcg aggatctagg
     1921 tagggcgctc gcagcggccc tgcgggacgc gaaaaggtga gaaaagccgg gcactgcccg
     1981 gctttatttt tgctgctgcg cgttccaggc cgcccacact cgtttgacct ggctcgggct
     2041 gcatccgacc agcttggccg tcttggcaat gctcgatccg ccggagcgaa gcgtgatgat
     2101 gcggtcgtgc atgccggcgt cacgtttgcg gccggtgtag cggccggcgg ccttcgccaa
     2161 ctggacaccc tgacgttgac gctcgcgccg atcctcgtag tcgtcgcggg ccatctgcaa
     2221 ggcgagcttc aaaagcatgt cctggacgga ttccagaacg attttcgcca ctccgttcgc
     2281 ctcggcggcc agctccgaca ggtccaccac gccaggcacg gccagcttgg cccctttggc
     2341 ccggatcgac gcaaccaggc gctcggcctc ggccaacggc aagcggctga tgcggtcgat
     2401 cttctccgca acgacgactt caccaggttg caggtccgcg atcatgcgca gcagctcggg
     2461 ccggtcggcg cgtgcgccgg acgccttctc gcggtagatg ccggcgacgt agtacccggc
     2521 ggcccgcgtg gccgctacaa ggctctcctg gcgttcaaga ttctgctcgt ccgtactggc
     2581 gcgcaggtag atgcgggcga ccttcaacct tcgtccctcc ggttgttgct ctcgcgtcgc
     2641 catttccacg gctcgacggc gtgcggatcg gaccagaggc cgacgcgctt gcctcgcgcc
     2701 tcctgttcga gccgcagcat ttcagggtcg gccgcgcggc cgtggaagcg ataggcccac
     2761 gccatgccct ggtgaaccat cgcggcgttg acgttgcgcg gctgcggcgg ccggctggcc
     2821 agctccatgt tgacccacac ggtgcccagc gtgcggccgt aacggtcggt gtccttctcg
     2881 tcgaccagga cgtgccggcg gaacaccatg ccggccagcg cctggcgcgc acgttcgccg
     2941 aaggcttgcc gcttttccgg cgcgtcaatg tccaccaggc gcacgcgcac cggctgcttg
     3001 tctaccagca cgtcgatggt gtcgccgtcg atgatgcgca cgacctcgcc gcgcagctcg
     3061 gcccatgccg gcgaggcaac gaccaggacg gccagcgcgg cagcggcgcg cagcatggcg
     3121 tagcttcggc gcttcatgcg tggccccatt gctgatgatc ggggtacgcc aggtgcagca
     3181 ctgcatcgaa attggccttg cagtagccgt ccagcgccac ccgcgagccg aacgccggcg
     3241 aaaggtactc gaccaggccg ggccggtcgc ggacctcgcg ccccaggacg tggatgcgcc
     3301 ggccgcgtgt gccgtcgggt ccaggcacga aggccagcgc ctcgatgttg aagtcgatgg
     3361 atagaagttg tcggtagtgc ttggccgccc tcatcgcgtc ccccttggtc aaattgggta
     3421 tacccatttg ggcctagtct agccggcatg gcgcattaca gcaatacgca atttaaatgc
     3481 gcctagcgca ttttcccgac cttaatgcgc ctcgcgctgt agcctcacgc ccacatatgt
     3541 gctaatgtgg ttacgtgtat tttatggagg ttatccaatg agccgcctga caatcgacat
     3601 gacggaccag cagcaccaga gcctgaaagc cctggccgcc ttgcagggca agaccattaa
     3661 gcaatacgcc ctcgaacgtc tgttccccgg tgacgctgat gccgatcagg catggcagga
     3721 actgaaaacc atgctgggga accgcatcaa cgatgggctt gccggcaagg tgtccaccaa
     3781 gagcgtcggc gaaattcttg atgaagaact cagcggggat cgcgcttgac ggcctacatc
     3841 ctcacggctg aggccgaagc cgatctacgc ggcatcatcc gctacacgcg ccgggagtgg
     3901 ggcgcggcgc aggtgcgccg ctatatcgct aagctggaac agggcatagc caggcttgcc
     3961 gccggcgaag gcccgtttaa ggacatgagc gaactctttc ccgcgctgcg gatggcccgc
     4021 tgcgaacacc actacgtttt ttgcctgccg cgtgcgggcg aacccgcgtt ggtcgtggcg
     4081 atcctgcatg agcgcatgga cctcatgacg cgacttgccg acaggctcaa gggctgattt
     4141 cagccgctaa aaatcgcgcc actcacaacg tcctgatggc gtacttaccc aaagaacagc
     4201 taggagaatc atttatgctc agcacacttc cacaagctca tgcaactttc ttgaaccgca
     4261 tccgcgatgc ggtcgcttcc gatgttcgct tccgcgctct tctgatcggc ggctcttacg
     4321 ttcacggagg actcgatgag cactccgatt tggatttcga catcgttgtt gaggacaact
     4381 gctacgcaga tgtcttgtct acacgcaagg attttgccga ggcactgccc ggcttcctca
     4441 acgcgttcac cggcgaacat gtaggagaac cgcgccttct gatctgccta tatggtccgc
     4501 cactgctaca catcggggga tcagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     4561 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     4621 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     4681 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     4741 cggtttgcgt attggaatta attcttgaag acgaaagggc ctcgtgatac gcctattttt
     4801 ataggttaat gtcatgataa taatggtttc ttagagctta tcggccagcc tcgcagagca
     4861 ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc
     4921 gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag
     4981 tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa
     5041 aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagatccg tcgatcgacc
     5101 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5161 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5221 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5281 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5341 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5401 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5461 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5521 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5581 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5641 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5701 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5761 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5821 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5881 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5941 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6001 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6061 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6121 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6181 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6241 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgggggatc aggaccgctg
     6301 ccggagcgca acccactcac tacagcagag ccatgtagac aacatcccct ccccctttcc
     6361 accgcgtcag acgcccgtag cagcccgcta cgggcttttt catgccctgc cctagcgtcc
     6421 aagcctcacg gccgcgctcg gcctctctgg cggccttctg gcgctcctgc tgcggcgtcc
     6481 gctcgtgggc cgtggcgcgg gtccgcgcgc cggcctcgtg cgcctggcgc tcgcgggcga
     6541 ggtccagggc ggccgtcttc acgttctgcc ttgcgcagat gagatagatc cgtcaattcc
     6601 ggcctatcgt ttccacgatc agcgatcggc tcgttgccct gcgccgctcc aaagcccgcg
     6661 acgcagcgcc ggcaggcaga gcaagtagag ggcagcgcct gcaatccatg cccacccgtt
     6721 ccacgttgtt atagaagccg catagatcgc cgtgaagagg aggggtccga cgatcgaggt
     6781 caggctggtg agcgccgcca gtgagccttg cagctgcccc tgacgttcct catccacctg
     6841 cctggacaac attgcttgca gcgccggcat tccgatgcca cccgaagcaa gcaggaccat
     6901 gatcgggaac gccatccatc cccgtgtcgc gaaggcaagc aggatgtagc ctgtgccgtc
     6961 ggcaatcatt ccgagcatga gtgcccgcct ttcgccgagc cgggcggcta cagggccggt
     7021 gatcattgcc tgggcgagtg aatgcagaat gccaaatgcg gcaagcgaaa tgccgatcgt
     7081 ggtcgcgtcc cagtgaaagc gatcctcgcc gaaaatgacc caaagcgcgg ccggcacctg
     7141 tccgacaagt tgcatgatga agaagaccgc catcagggcg gcgacgacgg tcatgccccg
     7201 ggcccaccgg aacgaagcga gcgggttgag agcctcccgg cgtaacggcc ggcgttcgcc
     7261 tttgtgcgac tccggcaaaa ggaaacagcc cgtcaggaaa ttgaggccgt tcaaggctgc
     7321 cgcggcgaag aacggagcgt ggggggagaa accgcccatc agcccaccga gcacaggtcc
     7381 cgcgaccatc ccgaacccga aacaggcgct catgaagccg aagtgccgcg cgcgctcatc
     7441 gccatcagtg atatcggcaa tataagcgcc ggctaccgcc ccagtcgccc cggtgatgcc
     7501 ggccacgatc cgcccgatat agagaaccca aaggaaaggc gctgtcgcca tgatggcgta
     7561 gtcgacagtg gcgccggcca gcgagacgag caagattggc cgccgcccga aacgatccga
     7621 cagcgcgccc agcacaggtg cgcaggcaaa ttgcaccaac gcatacagcg ccagcagaat
     7681 gccatagtgg gcggtgacgt cgttcgagtg aaccagatcg cgcaggaggc ccggcagcac
     7741 cggcataatc aggccgatgc cgacagcgtc gagcgcgaca gtgctcagaa ttacgatcag
     7801 gggtatgttg ggtttcacgt ctggcctccg gaccagcctc cgctggtccg attgaacgcg
     7861 cggattcttt atcactgata agttggtgga catattatgt ttatcagtga taaagtgtca
     7921 agcatgacaa agttgcagcc gaatacagtg atccgtgccg ccctggacct gttgaacgag
     7981 gtcggcgtag acggtctgac gacacgcaaa ctggcggaac ggttgggggt tcagcagccg
     8041 gcgctttact ggcacttcag gaacaagcgg gcgctgctcg acgcactggc cgaagccatg
     8101 ctggcggaga atcatacgca ttcggtgccg agagccgacg acgactggcg ctcatttctg
     8161 atcgggaatg cccgcagctt caggcaggcg ctgctcgcct accgcgatgg cgcgcgcatc
     8221 catgccggca cgcgaccggg cgcaccgcag atggaaacgg ccgacgcgca gcttcgcttc
     8281 ctctgcgagg cgggtttttc ggccggggac gccgtcaatg cgctgatgac aatcagctac
     8341 ttcactgttg gggccgtgct tgaggagcag gccggcgaca gcgatgccgg cgagcgcggc
     8401 ggcaccgttg aacaggctcc gctctcgccg ctgttgcggg ccgcgataga cgccttcgac
     8461 gaagccggtc cggacgcagc gttcgagcag ggactcgcgg tgattgtcga tggattggcg
     8521 aaaaggaggc tcgttgtcag gaacgttgaa ggaccgagaa agggtgacga ttgatcagga
     8581 ccgctgccgg agcgcaaccc actcactaca gcagagccat gtagacaaca tcccctcccc
     8641 ctttccaccg cgtcagacgc ccgtagcagc ccgctacggg ctttttcatg ccctgcccta
     8701 gcgtccaagc ctcacggccg cgctcggcct ctctggcggc cttctggcgc tcctgctgcg
     8761 gcgtccgctc gtgggccgtg gcgcgggtcc gcgcgccggc ctcgtgcgcc tggcgctcgc
     8821 gggcgaggtc cagggcggcc gtcttcacgt tctgccttgc gcagatgaga tagatcccgg
     8881 aattgacgga tctagcgtgg actcaaggct ctcgcgaatg gctcgcgttg gaaactttca
     8941 ttgacacttg aggggcaccg cagggaaatt ctcgtccttg cgagaaccgg ctatgtcgtg
     9001 ctgcgcatcg agcctgcgcc cttggcttgt ctcgcccctc tccgcgtcgc tacggggctt
     9061 ccagcgcctt tccgacgctc accgggctgg ttgccctcgc cgctgggctg gcggccgtct
     9121 atggccctgc aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac cgcggccgcc
     9181 ggcgttgtgg atacctcgcg gaaaacttgg ccctcactga cagatgaggg gcggacgttg
     9241 acacttgagg ggccgactca cccggcgcgg cgttgacaga tgaggggcag gctcgatttc
     9301 ggccggcgac gtggagctgg ccagcctcgc aaatcggcga aaacgcctga ttttacgcga
     9361 gtttcccaca gatgatgtgg acaagcctgg ggataagtgc cctgcggtat tgacacttga
     9421 ggggcgcgac tactgacaga tgaggggcgc gatccttgac acttgagggg cagagtgctg
     9481 acagatgagg ggcgcaccta ttgacatttg aggggctgtc cacaggcaga aaatccagca
     9541 tttgcaaggg tttccgcccg tttttcggcc accgctaacc tgtcttttaa cctgctttta
     9601 aaccaatatt tataaacctt gtttttaacc agggctgcgc cctgtgcgcg tgaccgcgca
     9661 cgccgaaggg gggtgccccc ccttctcgaa ccctcccggc ccgctaacgc gggcctccca
     9721 tccccccagg ggctgcgccc ctcggccgcg aacggcctca ccccaaaaat ggcagcgctg
     9781 gcagtccttg ccattgccgg gatcggggca gtaacgggat gggcgatcag cccgagcgcg
     9841 acgcccggaa gcattgacgt gccgcaggtg ctggcatcga cattcagcga ccaggtgccg
     9901 ggcagtgagg gcggcggcct gggtggcggc ctgcccttca cttcggccgt cggggcattc
     9961 acggacttca tggcggggcc ggcaattttt accttgggca ttcttggcat agtggtcgcg
    10021 ggtgccgtgc tcgtgttcgg gggtgaatta attccccgga tcgatccgtc agcttcacgc
    10081 tgccgcaagc actcagggcg caagggctgc taaaggaagc ggaacacgta gaaagccagt
    10141 ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa
    10201 aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac
    10261 tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag
    10321 gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc
    10381 aggggatcaa gatcgacgga tcgatccggg gaattccggg gcaatcccgc aaggagggtg
    10441 aatgaatcgg acgtttgacc ggaaggcata caggcaagaa ctgatcgacg cggggttttc
    10501 cgccgaggat gccgaaacca tcgcaagccg caccgtcatg cgtgcgcccc gcgaaacctt
    10561 ccagtccgtc ggctcgatgg tccagcaagc tacggccaag atcgagcgcg acagcgtgca
    10621 actggctccc cctgccctgc ccgcgccatc ggccgccgtg gagcgttcgc gtcgtctcga
    10681 acaggaggcg gcaggtttgg cgaagtcgat gaccatcgac acgcgaggaa ctatgacgac
    10741 caagaagcga aaaaccgccg gcgaggacct ggcaaaacag gtcagcgagg ccaagcaggc
    10801 cgcgttgctg aaacacacga agcagcagat caaggaaatg cagctttcct tgttcgatat
    10861 tgcgccgtgg ccggacacga tgcgagcgat gccaaacgac acggcccgct ctgccctgtt
    10921 caccacgcgc aacaagaaaa tcccgcgcga ggcgctgcaa aacaaggtca ttttccacgt
    10981 caacaaggac gtgaagatca cctacaccgg cgtcgagctg cgggccgacg atgacgaact
    11041 ggtgtggcag caggtgttgg agtacgcgaa gcgcacccct atcggcgagc cgatcacctt
    11101 cacgttctac gagctttgcc aggacctggg ctggtcgatc aatggccggt attacacgaa
    11161 ggccgaggaa tgcctgtcgc gcctacaggc gacggcgatg ggcttcacgt ccgaccgcgt
    11221 tgggcacctg gaatcggtgt cgctgctgca ccgcttccgc gtcctggacc gtggcaagaa
    11281 aacgtcccgt tgccaggtcc tgatcgacga ggaaatcgtc gtgctgtttg ctggcgacca
    11341 ctacacgaaa ttcatatggg agaagtaccg caagctgtcg ccgacggccc gacggatgtt
    11401 cgactatttc agctcgcacc gggagccgta cccgctcaag ctggaaacct tccgcctcat
    11461 gtgcggatcg gattccaccc gcgtgaagaa gtggcgcgag caggtcggcg aagcctgcga
    11521 agagttgcga ggcagcggcc tggtggaaca cgcctgggtc aatgatgacc tggtgcattg
    11581 caaacgctag ggccttgtgg ggtcagttcc ggctgggggt tcagcagcca cctgcaggtc
    11641 atcaactatc aaacgcttcg gttaagctta aagcacacat caaacgcttc ggttaagctt
    11701 aaagcacacc ctttctgcgt cctcgtattg acgcgacgta aaatttcaac gagcacgccg
    11761 ggatacttac catattctct gctaattatc ccgacatcat cggtaacaat aaatgctgga
    11821 taactggttg ctgacgcatc catataactc atcaaccccg gcgttccatc aggtacaggt
    11881 ttcaacgttt caggatcaag cgctcgcgca tatacccacg gcggaacatg tttacgctgc
    11941 atttcatcct caaagaaaca agtgttgagt tcaacttgat taaatatatc tcggatctga
    12001 ctaatatcac tgagattgaa agtatcaaat aaaagatgat tgaaatcatc acgtttcaga
    12061 gattcttttt cgtaactttt ccagccgcct ccggttatga tataaaggct tttatctcca
    12121 gaaaatgaga tttttttatc tttcatataa tggcagagta aataaataaa gtatggcgaa
    12181 ccaataagac aaagatcttt cccttgattt tttattcgtt caagactatt caatgtttta
    12241 acaaaatcta ttcgttcttc tgttacggta aatgtcgtag gatataacaa ttccaccaaa
    12301 ctcataacat atttaaacca aatattatga gcattaaatc tatctggtcc caaattgact
    12361 aattctattt gatgatcaaa ccaactacca acatatttca tgccataact cacagagcct
    12421 aagagtctct caatacttaa tctgtcacgc gccacctgac tttttaaacc attcgtgccg
    12481 ctactggtaa accaactttc aatctcgttt tcctgagaag ttaataagcg agtaaactta
    12541 aaaaccgatg ttgggaatac aggtatgtca tcaatttccg taatattgtc atctactttg
    12601 tgtgcctgac agtagtgacg atattctcga caatgtttat aatgattacg aaatgcatca
    12661 agcacaagtt tctttctgat tttttcctgc tcgtcgtaag accacactaa tggatcgctc
    12721 gaaaaaatca aatcatcaat ttctgagctt gctgtaattt cttgtttatc aacatatgaa
    12781 gtcatacctg ttttactcct caagataata ttagaaagta tggcagcact gctgtcatac
    12841 tcttttatac ccttcatctt tcaagctgct gctttgttgg ctgctttcac tcaccccagt
    12901 cacatagtta tctatgctcc tggggattcg ttcacttgcc gcctcgctgc aactcgaaat
    12961 ctattaggta tattccatgt ggtacttctt aatattatca tcaacaatat tgattacatt
    13021 tttttggctc atcaaatcat tcattggttc aaaggacagc aatacacttt tcgcaccaca
    13081 cttttcaatt gccaacttag ccgcagttat acactccgta taatttccga cagcgttttc
    13141 tgcaattatt tcttcaagtt tattttcgaa attttcatta gggtgcattt caagaacata
    13201 atcactaata aatgcacgcg tctcttgttt agctttatta ctatcttcgt tatagttaac
    13261 taatatcatt aactgatggt ctatctctga taggtcaacg tcatatttat ccgcaacggc
    13321 tttatatctt tcagcatatt catatctaac atcattagaa tcatcccact taaagatgag
    13381 aggaatacct tttttggccg cccactcaac aatatgatga ctggttgctg ttacatattt
    13441 ccgaggtccg cctggcgtat aagcatgggg atttacagat attttaggga agctataaaa
    13501 atcgttatct ggattacaat agcctgttgt taaagcatcg ttaatgattt cataacactc
    13561 ttcaaatagt tgctgttgat attcaaccgg gcgattaaaa aaatgcattt catctttttt
    13621 ttcgcaatca ctaaacccta aaataaatct cccttcactt aactgatcca ataagcaagc
    13681 ttcctccgct atgcggacag gatgatgagt tgtaatgatg tgatttaatg aaccaatttt
    13741 aattttctct gttaaaccga gcagaaaacc agaaacagtc agaggagcgc cgacaacacc
    13801 attatctgaa aaatgatttt catacactaa aatctgttca aaattcaact tatcaacata
    13861 ctccgttatt tcctgcatgc gaactatact ttgttcttga acagttgttg aattgatgaa
    13921 gttaaggaag aacaatccaa atttcatttc tttctcctta gctaatataa tagcgaacgt
    13981 tgtttttctt taagaaatgg catgacatca gactggaaga gcttcatgga agcaataatt
    14041 tcgtctactg ttccattagc ttcaaatcca caacaaatat ttgatattcc tgtagcatca
    14101 atgtcttttt gaattatgtc aatacattcc tgcggcgttc ccacgggatt gatttcgtaa
    14161 ctgtaatcaa tacggcgatt agtatcttta tgtcctttta atacaaagtc acgccactgc
    14221 cctttattga aatcataacc tcttgtttgg tctgaatcat caaaaatagt cgtagcattc
    14281 acataagaat cataccaatg ccccagaaat ttccggcaaa tctctttcgc tttaattgag
    14341 tcatgatcta cagatgttat atatgataag caatggtcga tattatgaat atcgtgccca
    14401 tattcttgag ccacttcatt ataaagctca agttgtgctt tcttttcgtt agtatttata
    14461 atccaactta atatcatcgg taggccaaat tgagcagccc actcagtcgt cgaagctgat
    14521 tcagccacca cataaaccgg tgcgccacct ctgctatacg ccgcggggtt tacttttacc
    14581 ttatggaact tgatatgttc attatcagct tccatatatc cctctgtcat gccattcttt
    14641 atcagcccgt accagcattc cgctaaggcg cgactgttat tcatatctgt gccgaatacg
    14701 cgaaagtcct tgttgtaaag ccctcggcaa ataccaaacc gaaatcgtcc ttttgacatt
    14761 tgatccaata aattcacatc ttcaagttgg cgtactggat gggctgtggg aagaacaata
    14821 gcggcagttc ctacattcaa ttttttagtc gcgccaagta aatatgcagc agcgacataa
    14881 gggttaccaa gcaaaccaaa ctccgtgaaa tgatgctcca gtaaccatac ggtatcaaaa
    14941 ccacactcct cagagatgcg acctaattta accaaacgtt tcattacctc tgtttgagaa
    15001 aattggggag gttggtatgt aagcaaaaag tttccaaatt tcatagagag tccttatatt
    15061 gctatttgag tgatagaata tctcaataga ttttaagaca gagaaattgc ttgattttca
    15121 atctcaattc tcattcggcg ttcattgact gtcgcaatag ttaaatgttc aaatgacggt
    15181 tcagtaatat caacatcaat atccagatga tcattatcca tcgcgatagc ggctttcgta
    15241 accgattgat aaaaattgcg caggaccact aaattttcac tcaagtcatg cgaacttcct
    15301 aacaaagaat atatcttgca tcgattacta cgaatatttg ataacaatgt gataacttca
    15361 tcttgcttga cccaattatc gttatttgca gtaaaagcaa taaacggtat atcaagatac
    15421 atcatgttat taattgtaga agctaaatct tcccaaccaa aatcaagaca atctctcgca
    15481 aagacttcag cacccaattt atggccttca aaatctagat tattcggcaa ttcattaatg
    15541 ggtagactga gataatcaaa ccctaaagct ctttcaagag aatatcttaa gttaacaaca
    15601 ccgactgcgg tgattaaaaa cgaagcattg atttcagata ggcttgcata agctatccgc
    15661 gcagataagc ttgaagccaa cataccgaag ttatttattt ttcgtgtagt taaccaatca
    15721 accactgcta acaagctctg ctttcctata gacattgtaa attcatcaat tgtccctgaa
    15781 ctcaatccaa cgtggtgaag cgaatcatag cggatcacat gaaatccatt ccgcgataaa
    15841 tattccgcca gaccagcaaa atgatccatc ctgcgggcaa aaccagacgc aataataatg
    15901 gcattctttc tctttgggct gttttcttct ggcagcgttt cccaaacatg aattttttta
    15961 tttccttcaa cacaaataac gtggtcgatg gttttatatt ttgattcatt ttccatactt
    16021 tttacctatt atgggacaaa tacaaggaac ttatcttctt ccaggaatcg agtctgttct
    16081 atttcaaccg caacatcctt agccgtatag ttagatggcc tttcatgaga aatatatgtc
    16141 actaatcgtt gcaacggtct cattccgtca tgagatccac caactcgaaa tatgttattc
    16201 attcctgctt ctacaatcct ttccgcacct tttaatgcta acgcatctcg atatttaaat
    16261 gatgactccc aaggaaaaat agatatggtt tgcgtcttat ttttttgaac ataaggcaat
    16321 atttgctcaa tattatcgac gtgatgaagg tacacacatc tgccaagtgg ttgattaaat
    16381 tccacacctg catttgactc aataatcatc caacgttgat gaatatccac ctctactttt
    16441 aatccagcaa acaagctttc tttttgaact aaagaatagg ccgccttttc atcaaaatct
    16501 tttttggcat tcggtaatat atgcgcatat agattaagtt tttctatcaa cgctaactta
    16561 aattcctcat aatgatttcc catgtaatat atgttttggg cagaaaaaca agctcgctga
    16621 tcgtaaaaac aaacatcatg agccgcacct gtcgctgcgg acgtcaaatc aacaggatta
    16681 tcgataatgc aaagactctt tttagaacca aatttaatca catcagcata agatggcgca
    16741 tgctctaccg cccaattaat cgcatctggc cctccccaag cgacaataac atccgcatgt
    16801 tgcataattt cttttgcgag tgatgtatca ccttggtggg gccaatatat aacagataaa
    16861 gagcgcgtta tcggatgatt agggtctaca tcaataaaac ttaacgctaa tgcattagcg
    16921 gtaaaaggat cggttgacga tgtttttata atacactgat tcttagttaa aattgcgcgt
    16981 aatatagaca tgatcccaga taatggaaca ttacctgcca acagatgtac agatttacct
    17041 ttcggaaaag cccgaacata actttcatcc tgaggtagcc attcatccat gatatggcga
    17101 gaaccaagtt cattttctac aacatcataa aggccgcctt tagaacataa aatcatagat
    17161 atccaattgg cctctagctt agccatttct tctgaatatc ccatatattt ttttaagtca
    17221 cgaatgtatg tcctgcgtct tgagtattct tcatttttcc atctttgccc taccgtatag
    17281 agaaaattga caatgttatg caaccgtaat tcgttatttc cattacaatc aataatgttt
    17341 tttacatgag agtcattcaa tattggcagg taaacactat tatcaccaaa attaatggat
    17401 tgcactaaat catcaccttc gggaaagatt tcaacctggc cgttaataat gaatgaaatt
    17461 tttttagtca tacgtatcct ccaagcctga attcgtttaa acg
//
