LOCUS       sequence-171207-        6270 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid encodes blinded measles virus H glycoprotein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Ou W, Marino MP, Suzuki A, Joshi B, Husain SR, Maisner A, Galanis E,
            Puri RK, Reiser J
  TITLE     Specific targeting of human interleukin (IL)-13 receptor 
            alpha2-positive cells with lentiviral vectors displaying IL-13.
  JOURNAL   Hum Gene Ther Methods. 2012 Apr;23(2):137-47. Epub 2012 May 21.
  PUBMED    22612657
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther Methods."; date: "2012-04"; volume: "23(2)"; pages: "137-47. 
            Epub 2012 May 21"
COMMENT     Sequence Label: pCG-Hc∆18-AA
FEATURES             Location/Qualifiers
     source          1..6270
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      73..208
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     544..563
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    562..617
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     663..682
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(881..898)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(2751..2769)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2797..2816)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          2824..3397
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(3497..3521)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(3518..3721)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3547..3567)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3722..4025
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(4049..4067)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4186..4205)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4305..4327
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4365..4383)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4451..4555
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4556..4624,4625..5416)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4774..4793)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5587..6175
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6076..6095
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctccccgcgc gttggccgat tcattaatgc agcaattagt cagcaaccat agtcccgccc
       61 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
      121 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
      181 aagtagtgaa gaggcttttt tggaggccta ggcttttgca aaaagctcct cgagggatct
      241 ccataagaga agagggacag ctatgactgg gagtagtcag gagaggagga aaaatctggc
      301 tagtaaaaca tgtaaggaaa attttaggga tgttaaagaa aaaaataaca caaaacaaaa
      361 tataaaaaaa atctaacctc aagtcaaggc ttttctatgg aataaggaat ggacagcagg
      421 gggctgtttc atatactgat gacctcttta tagccaacct ttgttcatgg cagccagcat
      481 atgggcatat gttgccaaac tctaaaccaa atactcattc tgatgtttta aatgatttgc
      541 cctcccatat gtccttccga gtgagagaca caaaaaattc caacacacta ttgcaatgaa
      601 aataaatttc ctttattagc cagaagtcag atgctcaagg ggcttcatga tgtccccata
      661 atttttggca gagggaaaaa gatcagcttg catgcctgca ggtttaaaca gtcgagcata
      721 ctccaggatg gctactatct tattggtaac tatcgggcta tctaggtgaa cttcagggta
      781 taagatctgg ttgacagata gcgagtccat aacggggaac cacttggacc ctacgttttt
      841 cttaattctg atgtctattt cacactagta caaactatca gtgatggtga tggtgatgcg
      901 atcctcttgc ggccgcttcc cgggtgactg tgcagctgac tcccatgccc accatcccag
      961 agtgagtgat atgtccacca gattctgagt ccgcaagcac acagaagtga cggcaccaga
     1021 gtttttggtc ccatgtgaag cattccactt gtaattcgat ggggaccccc tttataggca
     1081 acctaaaagg ataaaagtaa gaaaatgagc ggcttgggct gtaaacgtaa taaaccacag
     1141 catgttcaac cgcggaagta tcgtaggttg ccaaaacata ttggagatct tgaccaggta
     1201 gaatcaccag attggaactg agtttgacat caccatccac ctccgcaggt aggtatgttg
     1261 gggcatggca gtctccgcct gcttccttaa ttgggacatt gaagagcgcg ggactaacct
     1321 tgaatctcgg tatccactcc aatgtgttga ttacacctaa ggctaggttc ttcattggcg
     1381 ggatagtcag ccaatacaca ttgttgtggt tggatttgta taggtccatc cctgaaccgt
     1441 gtgtgatcaa tggcccgaat cccgaagcaa ttttgatttt aagctcaact gtcagactca
     1501 gatcaacaga caagaccccg tatgaaggaa tcctgttatc cttcaatggt gcccactcgg
     1561 gattctcgca gagtgcttgg attttaccct tacacgcctg ttggaagcat gtctccattc
     1621 gcaacttgtc atctgttcgt gttgtcggga cagcccattt tgcttggttg tcagcgataa
     1681 cacctctgtg agatgagagg taaagcctgt ctatcactgg atcatccgtt gataagggga
     1741 cccaggattg catgtcggtt ggggatttcc agacacctag cttgacgagc tggaagctga
     1801 cacctttccc tgatccctga tagggaattg tgatagaatc ttccccgtga caaagggctg
     1861 cgagtttgag ctcccccaaa gccaccatac agttgctgag atcattactg actggttgct
     1921 caagatagtt tgtcatatgg aacaccggag cccccaaacc cggatttctg ataacaccta
     1981 cttcaaacac tcggtacatg ctcagttgtg acaactctga ccttttgctg ctcagattag
     2041 gcttttccac taggtaagtt cccccataca ttccctggga tgtcatagtg actatagatg
     2101 acacattgta acctcgacct aaatacaagt ctaacaggga cagcgacatg tttgagaatt
     2161 gacctctgat tgtagtgggc cctgagcagt ttccctttga gacagctagg aactgattgg
     2221 ttgttctggt ctccagtaga gttgagttca ccaatgcatt catcagttcc tcggcggcca
     2281 catcggcgca gtactggtcg tagtccagct tgatccgctc gggggggttg atgcaccagg
     2341 tcaggtcccg gaagtcgtac tcccggtcgg ggttcaggaa cttgatcttg tcgctgatga
     2401 acttgaccag gtcggtgaat ctctgggggg tccgcaggcc cacttcgtcg ccgatgatct
     2461 taaacagggg ggtcagcacg tccttgacct gatgctcgat gctgttggtc acgtccaggt
     2521 tggtgctcag gctcttgtgg atctcggcgg tgtagatggc ggctctgtgc agccggattc
     2581 cagcaatggc tagcaagccg atcaggctca ggaacatcac gaacagcacg gccagcagca
     2641 cgtagggccg gtcgatcatc aggtgctccc ggttgatcac gatccggctg cccattatcg
     2701 atgatcttgc accctaattt ttaattaact accgggggat ccgaattcgc cctatagtga
     2761 gtcgtattac aattctttgc caaaatgatg agacagcaca ataaccagca cgttgcccag
     2821 gagctgtagg aaaaagaaga aggcatgaac atggttagca gaggggcccg gtttggactc
     2881 agagtatttt atcctcatct caaacagtgt atatcattgt aaccataaag agaaaggcag
     2941 gatgatgacc aggatgtagt tgtttctacc aataagaata tttccacgcc agccagaatt
     3001 tatatgcaga aatattctac cttatcattt aattataaca attgttctct aaaactgtgc
     3061 tgaagtacaa tataatatac cctgattgcc ttgaaaaaaa aagtgattag agaaagtact
     3121 tacaatctga caaataaaca aaagtgaatt taaaaattcg ttacaaatgc aagctaaagt
     3181 ttaacgaaaa agttacagaa aatgaaaaga aaataagagg agacaatggt tgtcaacaga
     3241 gtagaaagtg aaagaaacaa aattatcatg agggtccatg gtgatacaag ggacatcttc
     3301 ccattctaaa caacaccctg aaaactttgc cccctccata taacatgaat tttacaatag
     3361 cgaaaaagaa agaacaatca agggtcccca aactcaccct gaagttctca ggatcggggg
     3421 gatcgatccc ccggaggctg gatcggtccc ggtgtcttct atggaggtca aaacagcgtg
     3481 gatggcgtct ccaggcgatc tgacggttca ctaaacgagc tctgcttata tagacctccc
     3541 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
     3601 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
     3661 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
     3721 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     3781 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     3841 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     3901 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     3961 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     4021 taacggggcg agctcgaatt ccggtctccc tatagtgagt cgtattaatt tcgataagcc
     4081 agaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
     4141 atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctgctctga
     4201 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     4261 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     4321 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     4381 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     4441 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     4501 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     4561 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     4621 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     4681 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     4741 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     4801 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     4861 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     4921 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     4981 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5041 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5101 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5161 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     5221 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     5281 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     5341 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     5401 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     5461 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     5521 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     5581 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     5641 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     5701 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     5761 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     5821 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     5881 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     5941 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6001 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6061 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6121 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6181 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     6241 tcctgcgtta tcccctgatt ctgtggataa
//
