LOCUS       sequence-171210-       17168 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phenylalanine biosensor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17168)
  AUTHORS   Pini F, East AK, Appia-Ayme C, Tomek J, Karunakaran R, 
            Mendoza-Suarez M, Edwards A, Terpolilli JJ, Roworth J, Downie JA, 
            Poole PS
  TITLE     Bacterial Biosensors for in Vivo Spatiotemporal Mapping of Root 
            Secretion.
  JOURNAL   Plant Physiol. 2017 Jul;174(3):1289-1306. doi: 10.1104/pp.16.01302. 
            Epub 2017 May 11.
  PUBMED    28495892
REFERENCE   2  (bases 1 to 17168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.16.01302"; journalName: "Plant Physiol"; date: 
            "2017-07"; volume: "174"; issue: "3"; pages: "1289-1306"
COMMENT     Sequence Label: pLMB577
FEATURES             Location/Qualifiers
     source          1..17168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3296..3312)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3296..3312)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3309..3331)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3320..3336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3344..3350,3351..3368,3369..3374))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3389..3410
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3535..3553)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     oriT            3598..3706
                     /note="incP origin of transfer"
     promoter        3883..3987
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3988..4056,4057..4848)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4206..4225)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(5375..6574)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             6680..7330
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     rep_origin      7799..8510
                     /label=oriV
                     /note="incP origin of replication"
     CDS             9177..10325
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10408..11520)
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     CDS             complement(11699..12682)
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             complement(12697..13779)
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             complement(13828..14751)
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             complement(14764..16206)
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
ORIGIN
        1 ccccaccgcg gactctgact acaaccgtgc gcaaggcgca ttagggagga tgtatgtatg
       61 taatcgcctg cggcatcgtt gccggcttgg cggctgcggt ggccctgttg ggcttcacgc
      121 cgatgatgga ggcgcttgcc gccggcgaac gccgcaaggc actcgcgcaa tggacgcgga
      181 cgatgttcct ggtgctgctg cctgtcgtgc tgatgtgcgc gcccatcggg tccagcattt
      241 acgacgccgt gcaagcggac gctggcaagc ccatcgcttt ccacaacggc cggatcacgg
      301 tcgtcatggc cctggtcggc agcttggccg ttgtcctggt cgcggctgcg cgtgcggtgg
      361 tcaaccgcaa gcatgccagc ttctggttcg tcggctgggt gatggcgtcg gttttggccg
      421 gcggcgtcgg cgcgatcgcc agcgcgaagc aactggcgtt cctcggcgaa catagcggca
      481 tggtggcctt cggcttcttc cgcgaccagg tgaaggacat gcactgcgat gcggacgtga
      541 tcctggcccg gtgggatgaa aaggcgaact cgccggtggt ctaccgctgc ccgaaggcgt
      601 acctgctcaa caggttcgca tccgcgccct tcgtgccctg gccggactac accgaggggg
      661 aaagcgagga tctaggtagg gcgctcgcag cggccctgcg ggacgcgaaa aggtgagaaa
      721 agccgggcac tgcccggctt tatttttgct gctgcgcgtt ccaggccgcc cacactcgtt
      781 tgacctggct cgggctgcat ccgaccagct tggccgtctt ggcaatgctc gatccgccgg
      841 agcgaagcgt gatgatgcgg tcgtgcatgc cggcgtcacg tttgcggccg gtgtagcggc
      901 cggcggcctt cgccaactgg acaccctgac gttgacgctc gcgccgatcc tcgtagtcgt
      961 cgcgggccat ctgcaaggcg agcttcaaaa gcatgtcctg gacggattcc agaacgattt
     1021 tcgccactcc gttcgcctcg gcggccagct ccgacaggtc caccacgcca ggcacggcca
     1081 gcttggcccc tttggcccgg atcgacgcaa ccaggcgctc ggcctcggcc aacggcaagc
     1141 ggctgatgcg gtcgatcttc tccgcaacga cgacttcacc aggttgcagg tccgcgatca
     1201 tgcgcagcag ctcgggccgg tcggcgcgtg cgccggacgc cttctcgcgg tagatgccgg
     1261 cgacgtagta cccggcggcc cgcgtggccg ctacaaggct ctcctggcgt tcaagattct
     1321 gctcgtccgt actggcgcgc aggtagatgc gggcgacctt caaccttcgt ccctccggtt
     1381 gttgctctcg cgtcgccatt tccacggctc gacggcgtgc ggatcggacc agaggccgac
     1441 gcgcttgcct cgcgcctcct gttcgagccg cagcatttca gggtcggccg cgcggccgtg
     1501 gaagcgatag gcccacgcca tgccctggtg aaccatcgcg gcgttgacgt tgcgcggctg
     1561 cggcggccgg ctggccagct ccatgttgac ccacacggtg cccagcgtgc ggccgtaacg
     1621 gtcggtgtcc ttctcgtcga ccaggacgtg ccggcggaac accatgccgg ccagcgcctg
     1681 gcgcgcacgt tcgccgaagg cttgccgctt ttccggcgcg tcaatgtcca ccaggcgcac
     1741 gcgcaccggc tgcttgtcta ccagcacgtc gatggtgtcg ccgtcgatga tgcgcacgac
     1801 ctcgccgcgc agctcggccc atgccggcga ggcaacgacc aggacggcca gcgcggcagc
     1861 ggcgcgcagc atggcgtagc ttcggcgctt catgcgtggc cccattgctg atgatcgggg
     1921 tacgccaggt gcagcactgc atcgaaattg gccttgcagt agccgtccag cgccacccgc
     1981 gagccgaacg ccggcgaaag gtactcgacc aggccgggcc ggtcgcggac ctcgcgcccc
     2041 aggacgtgga tgcgccggcc gcgtgtgccg tcgggtccag gcacgaaggc cagcgcctcg
     2101 atgttgaagt cgatggatag aagttgtcgg tagtgcttgg ccgccctcat cgcgtccccc
     2161 ttggtcaaat tgggtatacc catttgggcc tagtctagcc ggcatggcgc attacagcaa
     2221 tacgcaattt aaatgcgcct agcgcatttt cccgacctta atgcgcctcg cgctgtagcc
     2281 tcacgcccac atatgtgcta atgtggttac gtgtatttta tggaggttat ccaatgagcc
     2341 gcctgacaat cgacatgacg gaccagcagc accagagcct gaaagccctg gccgccttgc
     2401 agggcaagac cattaagcaa tacgccctcg aacgtctgtt ccccggtgac gctgatgccg
     2461 atcaggcatg gcaggaactg aaaaccatgc tggggaaccg catcaacgat gggcttgccg
     2521 gcaaggtgtc caccaagagc gtcggcgaaa ttcttgatga agaactcagc ggggatcgcg
     2581 cttgacggcc tacatcctca cggctgaggc cgaagccgat ctacgcggca tcatccgcta
     2641 cacgcgccgg gagtggggcg cggcgcaggt gcgccgctat atcgctaagc tggaacaggg
     2701 catagccagg cttgccgccg gcgaaggccc gtttaaggac atgagcgaac tctttcccgc
     2761 gctgcggatg gcccgctgcg aacaccacta cgttttttgc ctgccgcgtg cgggcgaacc
     2821 cgcgttggtc gtggcgatcc tgcatgagcg catggacctc atgacgcgac ttgccgacag
     2881 gctcaagggc tgatttcagc cgctaaaaat cgcgccactc acaacgtcct gatggcgtac
     2941 ttacccaaag aacagctagg agaatcattt atgctcagca cacttccaca agctcatgca
     3001 actttcttga accgcatccg cgatgcggtc gcttccgatg ttcgcttccg cgctcttctg
     3061 atcggcggct cttacgttca cggaggactc gatgagcact ccgatttgga tttcgacatc
     3121 gttgttgagg acaactgcta cgcagatgtc ttgtctacac gcaaggattt tgccgaggca
     3181 ctgcccggct tcctcaacgc gttcaccggc gaacatgtag gagaaccgcg ccttctgatc
     3241 tgcctatatg gtccgccact gctacacatc gggggatcag cttggcgtaa tcatggtcat
     3301 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     3361 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     3421 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     3481 aacgcgcggg gagaggcggt ttgcgtattg gaattaattc ttgaagacga aagggcctcg
     3541 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag agcttatcgg
     3601 ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa
     3661 gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg
     3721 gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa
     3781 ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa
     3841 gatccgtcga tcgacccagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     3901 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3961 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4021 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4081 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4141 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4201 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     4261 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     4321 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     4381 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     4441 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     4501 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     4561 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     4621 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     4681 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4741 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     4801 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     4861 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4921 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4981 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgg
     5041 gggatcagga ccgctgccgg agcgcaaccc actcactaca gcagagccat gtagacaaca
     5101 tcccctcccc ctttccaccg cgtcagacgc ccgtagcagc ccgctacggg ctttttcatg
     5161 ccctgcccta gcgtccaagc ctcacggccg cgctcggcct ctctggcggc cttctggcgc
     5221 tcctgctgcg gcgtccgctc gtgggccgtg gcgcgggtcc gcgcgccggc ctcgtgcgcc
     5281 tggcgctcgc gggcgaggtc cagggcggcc gtcttcacgt tctgccttgc gcagatgaga
     5341 tagatccgtc aattccggcc tatcgtttcc acgatcagcg atcggctcgt tgccctgcgc
     5401 cgctccaaag cccgcgacgc agcgccggca ggcagagcaa gtagagggca gcgcctgcaa
     5461 tccatgccca cccgttccac gttgttatag aagccgcata gatcgccgtg aagaggaggg
     5521 gtccgacgat cgaggtcagg ctggtgagcg ccgccagtga gccttgcagc tgcccctgac
     5581 gttcctcatc cacctgcctg gacaacattg cttgcagcgc cggcattccg atgccacccg
     5641 aagcaagcag gaccatgatc gggaacgcca tccatccccg tgtcgcgaag gcaagcagga
     5701 tgtagcctgt gccgtcggca atcattccga gcatgagtgc ccgcctttcg ccgagccggg
     5761 cggctacagg gccggtgatc attgcctggg cgagtgaatg cagaatgcca aatgcggcaa
     5821 gcgaaatgcc gatcgtggtc gcgtcccagt gaaagcgatc ctcgccgaaa atgacccaaa
     5881 gcgcggccgg cacctgtccg acaagttgca tgatgaagaa gaccgccatc agggcggcga
     5941 cgacggtcat gccccgggcc caccggaacg aagcgagcgg gttgagagcc tcccggcgta
     6001 acggccggcg ttcgcctttg tgcgactccg gcaaaaggaa acagcccgtc aggaaattga
     6061 ggccgttcaa ggctgccgcg gcgaagaacg gagcgtgggg ggagaaaccg cccatcagcc
     6121 caccgagcac aggtcccgcg accatcccga acccgaaaca ggcgctcatg aagccgaagt
     6181 gccgcgcgcg ctcatcgcca tcagtgatat cggcaatata agcgccggct accgccccag
     6241 tcgccccggt gatgccggcc acgatccgcc cgatatagag aacccaaagg aaaggcgctg
     6301 tcgccatgat ggcgtagtcg acagtggcgc cggccagcga gacgagcaag attggccgcc
     6361 gcccgaaacg atccgacagc gcgcccagca caggtgcgca ggcaaattgc accaacgcat
     6421 acagcgccag cagaatgcca tagtgggcgg tgacgtcgtt cgagtgaacc agatcgcgca
     6481 ggaggcccgg cagcaccggc ataatcaggc cgatgccgac agcgtcgagc gcgacagtgc
     6541 tcagaattac gatcaggggt atgttgggtt tcacgtctgg cctccggacc agcctccgct
     6601 ggtccgattg aacgcgcgga ttctttatca ctgataagtt ggtggacata ttatgtttat
     6661 cagtgataaa gtgtcaagca tgacaaagtt gcagccgaat acagtgatcc gtgccgccct
     6721 ggacctgttg aacgaggtcg gcgtagacgg tctgacgaca cgcaaactgg cggaacggtt
     6781 gggggttcag cagccggcgc tttactggca cttcaggaac aagcgggcgc tgctcgacgc
     6841 actggccgaa gccatgctgg cggagaatca tacgcattcg gtgccgagag ccgacgacga
     6901 ctggcgctca tttctgatcg ggaatgcccg cagcttcagg caggcgctgc tcgcctaccg
     6961 cgatggcgcg cgcatccatg ccggcacgcg accgggcgca ccgcagatgg aaacggccga
     7021 cgcgcagctt cgcttcctct gcgaggcggg tttttcggcc ggggacgccg tcaatgcgct
     7081 gatgacaatc agctacttca ctgttggggc cgtgcttgag gagcaggccg gcgacagcga
     7141 tgccggcgag cgcggcggca ccgttgaaca ggctccgctc tcgccgctgt tgcgggccgc
     7201 gatagacgcc ttcgacgaag ccggtccgga cgcagcgttc gagcagggac tcgcggtgat
     7261 tgtcgatgga ttggcgaaaa ggaggctcgt tgtcaggaac gttgaaggac cgagaaaggg
     7321 tgacgattga tcaggaccgc tgccggagcg caacccactc actacagcag agccatgtag
     7381 acaacatccc ctcccccttt ccaccgcgtc agacgcccgt agcagcccgc tacgggcttt
     7441 ttcatgccct gccctagcgt ccaagcctca cggccgcgct cggcctctcc tgtctcttat
     7501 acctcgtggg ccgtggcgcg ggtccgcgcg ccggcctcgt gcgcctggcg ctcgcgggcg
     7561 aggtccaggg cggccgtctt cacgttctgc cttgcgcaga tgagatagat cccggaattg
     7621 acggatctag cgtggactca aggctctcgc gaatggctcg cgttggaaac tttcattgac
     7681 acttgagggg caccgcaggg aaattctcgt ccttgcgaga accggctatg tcgtgctgcg
     7741 catcgagcct gcgcccttgg cttgtctcgc ccctctccgc gtcgctacgg ggcttccagc
     7801 gcctttccga cgctcaccgg gctggttgcc ctcgccgctg ggctggcggc cgtctatggc
     7861 cctgcaaacg cgccagaaac gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt
     7921 tgtggatacc tcgcggaaaa cttggccctc actgacagat gaggggcgga cgttgacact
     7981 tgaggggccg actcacccgg cgcggcgttg acagatgagg ggcaggctcg atttcggccg
     8041 gcgacgtgga gctggccagc ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc
     8101 ccacagatga tgtggacaag cctggggata agtgccctgc ggtattgaca cttgaggggc
     8161 gcgactactg acagatgagg ggcgcgatcc ttgacacttg aggggcagag tgctgacaga
     8221 tgaggggcgc acctattgac atttgagggg ctgtccacag gcagaaaatc cagcatttgc
     8281 aagggtttcc gcccgttttt cggccaccgc taacctgtct tttaacctgc ttttaaacca
     8341 atatttataa accttgtttt taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg
     8401 aaggggggtg cccccccttc tcgaaccctc ccggcccgct aacgcgggcc tcccatcccc
     8461 ccaggggctg cgcccctcgg ccgcgaacgg cctcacccca aaaatggcag cgctggcagt
     8521 ccttgccatt gccgggatcg gggcagtaac gggatgggcg atcagcccga gcgcgacgcc
     8581 cggaagcatt gacgtgccgc aggtgctggc atcgacattc agcgaccagg tgccgggcag
     8641 tgagggcggc ggcctgggtg gcggcctgcc cttcacttcg gccgtcgggg cattcacgga
     8701 cttcatggcg gggccggcaa tttttacctt gggcattctt ggcatagtgg tcgcgggtgc
     8761 cgtgctcgtg ttcgggggtg aattaattcc ccggatcgat ccgtcagctt cacgctgccg
     8821 caagcactca gggcgcaagg gctgctaaag gaagcggaac acgtagaaag ccagtccgca
     8881 gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc
     8941 aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc
     9001 ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg
     9061 gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg
     9121 atcaagatcg acggatcgat ccggggaatt ccggggcaat cccgcaagga gggtgaatga
     9181 atcggacgtt tgaccggaag gcatacaggc aagaactgat cgacgcgggg ttttccgccg
     9241 aggatgccga aaccatcgca agccgcaccg tcatgcgtgc gccccgcgaa accttccagt
     9301 ccgtcggctc gatggtccag caagctacgg ccaagatcga gcgcgacagc gtgcaactgg
     9361 ctccccctgc cctgcccgcg ccatcggccg ccgtggagcg ttcgcgtcgt ctcgaacagg
     9421 aggcggcagg tttggcgaag tcgatgacca tcgacacgcg aggaactatg acgaccaaga
     9481 agcgaaaaac cgccggcgag gacctggcaa aacaggtcag cgaggccaag caggccgcgt
     9541 tgctgaaaca cacgaagcag cagatcaagg aaatgcagct ttccttgttc gatattgcgc
     9601 cgtggccgga cacgatgcga gcgatgccaa acgacacggc ccgctctgcc ctgttcacca
     9661 cgcgcaacaa gaaaatcccg cgcgaggcgc tgcaaaacaa ggtcattttc cacgtcaaca
     9721 aggacgtgaa gatcacctac accggcgtcg agctgcgggc cgacgatgac gaactggtgt
     9781 ggcagcaggt gttggagtac gcgaagcgca cccctatcgg cgagccgatc accttcacgt
     9841 tctacgagct ttgccaggac ctgggctggt cgatcaatgg ccggtattac acgaaggccg
     9901 aggaatgcct gtcgcgccta caggcgacgg cgatgggctt cacgtccgac cgcgttgggc
     9961 acctggaatc ggtgtcgctg ctgcaccgct tccgcgtcct ggaccgtggc aagaaaacgt
    10021 cccgttgcca ggtcctgatc gacgaggaaa tcgtcgtgct gtttgctggc gaccactaca
    10081 cgaaattcat atgggagaag taccgcaagc tgtcgccgac ggcccgacgg atgttcgact
    10141 atttcagctc gcaccgggag ccgtacccgc tcaagctgga aaccttccgc ctcatgtgcg
    10201 gatcggattc cacccgcgtg aagaagtggc gcgagcaggt cggcgaagcc tgcgaagagt
    10261 tgcgaggcag cggcctggtg gaacacgcct gggtcaatga tgacctggtg cattgcaaac
    10321 gctagggcct tggrsskksw gywyygsmwr sgskwkggcc ttgtggggtc agttccggct
    10381 gggggttcag cagccacctg caggtcatca actatcaaac gcttcggtta agcttaaagc
    10441 acaccctttc tgcgtcctcg tattgacgcg acgtaaaatt tcaacgagca cgccgggata
    10501 cttaccatat tctctgctaa ttatcccgac atcatcggta acaataaatg ctggataact
    10561 ggttgctgac gcatccatat aactcatcaa ccccggcgtt ccatcaggta caggtttcaa
    10621 cgtttcagga tcaagcgctc gcgcatatac ccacggcgga acatgtttac gctgcatttc
    10681 atcctcaaag aaacaagtgt tgagttcaac ttgattaaat atatctcgga tctgactaat
    10741 atcactgaga ttgaaagtat caaataaaag atgattgaaa tcatcacgtt tcagagattc
    10801 tttttcgtaa cttttccagc cgcctccggt tatgatataa aggcttttat ctccagaaaa
    10861 tgagattttt ttatctttca tataatggca gagtaaataa ataaagtatg gcgaaccaat
    10921 aagacaaaga tctttccctt gattttttat tcgttcaaga ctattcaatg ttttaacaaa
    10981 atctattcgt tcttctgtta cggtaaatgt cgtaggatat aacaattcca ccaaactcat
    11041 aacatattta aaccaaatat tatgagcatt aaatctatct ggtcccaaat tgactaattc
    11101 tatttgatga tcaaaccaac taccaacata tttcatgcca taactcacag agcctaagag
    11161 tctctcaata cttaatctgt cacgcgccac ctgacttttt aaaccattcg tgccgctact
    11221 ggtaaaccaa ctttcaatct cgttttcctg agaagttaat aagcgagtaa acttaaaaac
    11281 cgatgttggg aatacaggta tgtcatcaat ttccgtaata ttgtcatcta ctttgtgtgc
    11341 ctgacagtag tgacgatatt ctcgacaatg tttataatga ttacgaaatg catcaagcac
    11401 aagtttcttt ctgatttttt cctgctcgtc gtaagaccac actaatggat cgctcgaaaa
    11461 aatcaaatca tcaatttctg agcttgctgt aatttcttgt ttatcaacat atgaagtcat
    11521 acctgtttta ctcctcaaga taatattaga aagtatggca gcactgctgt catactcttt
    11581 tatacccttc atctttcaag ctgctgcttt gttggctgct ttcactcacc ccagtcacat
    11641 agttatctat gctcctgggg attcgttcac ttgccgcctc gctgcaactc gaaatctatt
    11701 aggtatattc catgtggtac ttcttaatat tatcatcaac aatattgatt acattttttt
    11761 ggctcatcaa atcattcatt ggttcaaagg acagcaatac acttttcgca ccacactttt
    11821 caattgccaa cttagccgca gttatacact ccgtataatt tccgacagcg ttttctgcaa
    11881 ttatttcttc aagtttattt tcgaaatttt cattagggtg catttcaaga acataatcac
    11941 taataaatgc acgcgtctct tgtttagctt tattactatc ttcgttatag ttaactaata
    12001 tcattaactg atggtctatc tctgataggt caacgtcata tttatccgca acggctttat
    12061 atctttcagc atattcatat ctaacatcat tagaatcatc ccacttaaag atgagaggaa
    12121 tacctttttt ggccgcccac tcaacaatat gatgactggt tgctgttaca tatttccgag
    12181 gtccgcctgg cgtataagca tggggattta cagatatttt agggaagcta taaaaatcgt
    12241 tatctggatt acaatagcct gttgttaaag catcgttaat gatttcataa cactcttcaa
    12301 atagttgctg ttgatattca accgggcgat taaaaaaatg catttcatct tttttttcgc
    12361 aatcactaaa ccctaaaata aatctccctt cacttaactg atccaataag caagcttcct
    12421 ccgctatgcg gacaggatga tgagttgtaa tgatgtgatt taatgaacca attttaattt
    12481 tctctgttaa accgagcaga aaaccagaaa cagtcagagg agcgccgaca acaccattat
    12541 ctgaaaaatg attttcatac actaaaatct gttcaaaatt caacttatca acatactccg
    12601 ttatttcctg catgcgaact atactttgtt cttgaacagt tgttgaattg atgaagttaa
    12661 ggaagaacaa tccaaatttc atttctttct ccttagctaa tataatagcg aacgttgttt
    12721 ttctttaaga aatggcatga catcagactg gaagagcttc atggaagcaa taatttcgtc
    12781 tactgttcca ttagcttcaa atccacaaca aatatttgat attcctgtag catcaatgtc
    12841 tttttgaatt atgtcaatac attcctgcgg cgttcccacg ggattgattt cgtaactgta
    12901 atcaatacgg cgattagtat ctttatgtcc ttttaataca aagtcacgcc actgcccttt
    12961 attgaaatca taacctcttg tttggtctga atcatcaaaa atagtcgtag cattcacata
    13021 agaatcatac caatgcccca gaaatttccg gcaaatctct ttcgctttaa ttgagtcatg
    13081 atctacagat gttatatatg ataagcaatg gtcgatatta tgaatatcgt gcccatattc
    13141 ttgagccact tcattataaa gctcaagttg tgctttcttt tcgttagtat ttataatcca
    13201 acttaatatc atcggtaggc caaattgagc agcccactca gtcgtcgaag ctgattcagc
    13261 caccacataa accggtgcgc cacctctgct atacgccgcg gggtttactt ttaccttatg
    13321 gaacttgata tgttcattat cagcttccat atatccctct gtcatgccat tctttatcag
    13381 cccgtaccag cattccgcta aggcgcgact gttattcata tctgtgccga atacgcgaaa
    13441 gtccttgttg taaagccctc ggcaaatacc aaaccgaaat cgtccttttg acatttgatc
    13501 caataaattc acatcttcaa gttggcgtac tggatgggct gtgggaagaa caatagcggc
    13561 agttcctaca ttcaattttt tagtcgcgcc aagtaaatat gcagcagcga cataagggtt
    13621 accaagcaaa ccaaactccg tgaaatgatg ctccagtaac catacggtat caaaaccaca
    13681 ctcctcagag atgcgaccta atttaaccaa acgtttcatt acctctgttt gagaaaattg
    13741 gggaggttgg tatgtaagca aaaagtttcc aaatttcata gagagtcctt atattgctat
    13801 ttgagtgata gaatatctca atagatttta agacagagaa attgcttgat tttcaatctc
    13861 aattctcatt cggcgttcat tgactgtcgc aatagttaaa tgttcaaatg acggttcagt
    13921 aatatcaaca tcaatatcca gatgatcatt atccatcgcg atagcggctt tcgtaaccga
    13981 ttgataaaaa ttgcgcagga ccactaaatt ttcactcaag tcatgcgaac ttcctaacaa
    14041 agaatatatc ttgcatcgat tactacgaat atttgataac aatgtgataa cttcatcttg
    14101 cttgacccaa ttatcgttat ttgcagtaaa agcaataaac ggtatatcaa gatacatcat
    14161 gttattaatt gtagaagcta aatcttccca accaaaatca agacaatctc tcgcaaagac
    14221 ttcagcaccc aatttatggc cttcaaaatc tagattattc ggcaattcat taatgggtag
    14281 actgagataa tcaaacccta aagctctttc aagagaatat cttaagttaa caacaccgac
    14341 tgcggtgatt aaaaacgaag cattgatttc agataggctt gcataagcta tccgcgcaga
    14401 taagcttgaa gccaacatac cgaagttatt tatttttcgt gtagttaacc aatcaaccac
    14461 tgctaacaag ctctgctttc ctatagacat tgtaaattca tcaattgtcc ctgaactcaa
    14521 tccaacgtgg tgaagcgaat catagcggat cacatgaaat ccattccgcg ataaatattc
    14581 cgccagacca gcaaaatgat ccatcctgcg ggcaaaacca gacgcaataa taatggcatt
    14641 ctttctcttt gggctgtttt cttctggcag cgtttcccaa acatgaattt ttttatttcc
    14701 ttcaacacaa ataacgtggt cgatggtttt atattttgat tcattttcca tactttttac
    14761 ctattatggg acaaatacaa ggaacttatc ttcttccagg aatcgagtct gttctatttc
    14821 aaccgcaaca tccttagccg tatagttaga tggcctttca tgagaaatat atgtcactaa
    14881 tcgttgcaac ggtctcattc cgtcatgaga tccaccaact cgaaatatgt tattcattcc
    14941 tgcttctaca atcctttccg caccttttaa tgctaacgca tctcgatatt taaatgatga
    15001 ctcccaagga aaaatagata tggtttgcgt cttatttttt tgaacataag gcaatatttg
    15061 ctcaatatta tcgacgtgat gaaggtacac acatctgcca agtggttgat taaattccac
    15121 acctgcattt gactcaataa tcatccaacg ttgatgaata tccacctcta cttttaatcc
    15181 agcaaacaag ctttcttttt gaactaaaga ataggccgcc ttttcatcaa aatctttttt
    15241 ggcattcggt aatatatgcg catatagatt aagtttttct atcaacgcta acttaaattc
    15301 ctcataatga tttcccatgt aatatatgtt ttgggcagaa aaacaagctc gctgatcgta
    15361 aaaacaaaca tcatgagccg cacctgtcgc tgcggacgtc aaatcaacag gattatcgat
    15421 aatgcaaaga ctctttttag aaccaaattt aatcacatca gcataagatg gcgcatgctc
    15481 taccgcccaa ttaatcgcat ctggccctcc ccaagcgaca ataacatccg catgttgcat
    15541 aatttctttt gcgagtgatg tatcaccttg gtggggccaa tatataacag ataaagagcg
    15601 cgttatcgga tgattagggt ctacatcaat aaaacttaac gctaatgcat tagcggtaaa
    15661 aggatcggtt gacgatgttt ttataataca ctgattctta gttaaaattg cgcgtaatat
    15721 agacatgatc ccagataatg gaacattacc tgccaacaga tgtacagatt tacctttcgg
    15781 aaaagcccga acataacttt catcctgagg tagccattca tccatgatat ggcgagaacc
    15841 aagttcattt tctacaacat cataaaggcc gcctttagaa cataaaatca tagatatcca
    15901 attggcctct agcttagcca tttcttctga atatcccata tattttttta agtcacgaat
    15961 gtatgtcctg cgtcttgagt attcttcatt tttccatctt tgccctaccg tatagagaaa
    16021 attgacaatg ttatgcaacc gtaattcgtt atttccatta caatcaataa tgttttttac
    16081 atgagagtca ttcaatattg gcaggtaaac actattatca ccaaaattaa tggattgcac
    16141 taaatcatca ccttcgggaa agatttcaac ctggccgtta ataatgaatg aaattttttt
    16201 agtcatacgt atcctccaag cctgaattcg tttaaacgga tcccttatcg ggcttcagcc
    16261 ccagcatttt gacgccatcc agatattcgc ggcaggcggt atcggaaagc aatttcatct
    16321 gacgccgata gagttcaccc cagatcgcat cttcatccgg ggtgtagtca tagagaccat
    16381 ccgggccggg caatttggcg gtgtagctgc tttccttggt catgtgctga tcctcccagg
    16441 cacggcgcta ttcctgccat tatgatccga gtttggagga ttttcttttc tttcatgctg
    16501 gctatcgcca taatggtggc aaatattccc gaaagtgacg ccaaaaatga aagaacatgg
    16561 gaatttccgc cgtaagcttc tgcagctcgt ccaggccgac ggcagtctgt cgttggccga
    16621 cctagcggaa aaggccggca tgtcgcagag ctcggcatgg cggaagatcc aggaactgga
    16681 agccgacggc gtcatccgta agcgggtgac gctgctcgat cctggaaaac tcgatctcaa
    16741 gctctgcgtt attgcgcatg tcacgctgga ggatcaccac gaagaggcgg tcgcatcttt
    16801 cgcttcgatg gtgctggagc ggccggagat catggagtgc tacgccctat caggcgcctt
    16861 cgattacatg ctgaagatcc gggcaagcga tgtggaaagc tacgaagcct tcatgacccg
    16921 ctatctcatg cgcaacccgc atgtgcgtac cgtcgtctcg agcttcgtgc tgcgcgagct
    16981 gaaattctcg actgaactac cgctctaatg cctgtccccg aacgaggcag cggaatcatc
    17041 gcgttctgtc gccatgtcag tgagccggca tcggcaccgg aaagccttcg aatgtcttca
    17101 gcgtgtcgag gacgatcgac gtcttcacgt gctgcaccga ctcgtggggc aacagaacgt
    17161 cgtggtac
//
