LOCUS       sequence-171212-       17341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tartrate biosensor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17341)
  AUTHORS   Pini F, East AK, Appia-Ayme C, Tomek J, Karunakaran R, 
            Mendoza-Suarez M, Edwards A, Terpolilli JJ, Roworth J, Downie JA, 
            Poole PS
  TITLE     Bacterial Biosensors for in Vivo Spatiotemporal Mapping of Root 
            Secretion.
  JOURNAL   Plant Physiol. 2017 Jul;174(3):1289-1306. doi: 10.1104/pp.16.01302. 
            Epub 2017 May 11.
  PUBMED    28495892
REFERENCE   2  (bases 1 to 17341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.16.01302"; journalName: "Plant Physiol"; date: 
            "2017-07"; volume: "174"; issue: "3"; pages: "1289-1306"
COMMENT     Sequence Label: pLMB708
FEATURES             Location/Qualifiers
     source          1..17341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4378..4394)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4378..4394)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4391..4413)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4402..4418
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4426..4432,4433..4450,4451..4456))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4471..4492
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4617..4635)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     oriT            4680..4788
                     /note="incP origin of transfer"
     promoter        4965..5069
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5070..5138,5139..5930)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5288..5307)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(6457..7656)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             7762..8412
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     rep_origin      8902..9613
                     /label=oriV
                     /note="incP origin of replication"
     CDS             10280..11428
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11514..12623)
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     CDS             complement(12802..13785)
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             complement(13800..14882)
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             complement(14931..15854)
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             complement(15867..17309)
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
ORIGIN
        1 gatcctaaac gaagcgcacc tcctgcgctt gcgaatatct atgaccaccg ccggcttgat
       61 cctcaagcga cacaggaata tataaaaatt tgatataatc gaattctgga gcagaacttc
      121 gtggacctga accagcttcg atgtttcgtg tcggtagcgg atgagcttca tttcgggcgg
      181 gccgctcaaa agcttggcct cctgccggcg tcgctatcga ggcacatacg cctgcttgaa
      241 gaatcccttg ggacgcggct gatggcgcga acgacacgca gcgtgtcgct gacggaggac
      301 ggcctcgtct tcctggaggg cgcgcgggag atcctgacca agaccgaagc tcttcagcag
      361 cgcttccaca cttcccgcca ggcaagagcg gatgtgctgc ggatcggggc gatcgacagc
      421 gcggcagcag ggctcgtccc catgctgctg cacgatttcc gccagcagtg tcccgacgtc
      481 tcgacgcaat tgtcggaaga caagacgatc aggctgatcc cgaaacttct gtcgggttca
      541 ttggatcttg cgttcatccg cgcccccgat atcgtcgatc ccaagctgaa gacgatgttt
      601 ctcttccatg agaccgcggt cgtcgctatg tccgagaacc atcgactggc ttcgagagcc
      661 agcgtgagaa tcgaagacct cgccgacgaa ccgttgatcg tcccggagcg ccggtcgcgc
      721 ccgcatagcc atgacctgac gatgaagctg ttcatcgagg cgggactgca ggcacgggtg
      781 gcccagcttg ccgaagaaaa gcagacgatt gtcaacctcg tggctgcgga gatcgggctg
      841 gcgatcgtac cccaatggac atcacggctc gccgtcagcg gcgtccgcta catcgctctg
      901 gatatgggag gaacggcggc gatgaacaag cttcccctat cggtcgcgtg gttgaagggc
      961 gtccgcgacc cgggacgaga tcgcattctc gccatgcttc aagacaagct ggcccactat
     1021 gccgagcttg cctgacacga gggcatccaa tgcgtttcac ctttgaatgt gcttagaggt
     1081 acccccaccg cggactctga ctacaaccgt gcgcaaggcg cattagggag gatgtatgta
     1141 tgtaatcgcc tgcggcatcg ttgccggctt ggcggctgcg gtggccctgt tgggcttcac
     1201 gccgatgatg gaggcgcttg ccgccggcga acgccgcaag gcactcgcgc aatggacgcg
     1261 gacgatgttc ctggtgctgc tgcctgtcgt gctgatgtgc gcgcccatcg ggtccagcat
     1321 ttacgacgcc gtgcaagcgg acgctggcaa gcccatcgct ttccacaacg gccggatcac
     1381 ggtcgtcatg gccctggtcg gcagcttggc cgttgtcctg gtcgcggctg cgcgtgcggt
     1441 ggtcaaccgc aagcatgcca gcttctggtt cgtcggctgg gtgatggcgt cggttttggc
     1501 cggcggcgtc ggcgcgatcg ccagcgcgaa gcaactggcg ttcctcggcg aacatagcgg
     1561 catggtggcc ttcggcttct tccgcgacca ggtgaaggac atgcactgcg atgcggacgt
     1621 gatcctggcc cggtgggatg aaaaggcgaa ctcgccggtg gtctaccgct gcccgaaggc
     1681 gtacctgctc aacaggttcg catccgcgcc cttcgtgccc tggccggact acaccgaggg
     1741 ggaaagcgag gatctaggta gggcgctcgc agcggccctg cgggacgcga aaaggtgaga
     1801 aaagccgggc actgcccggc tttatttttg ctgctgcgcg ttccaggccg cccacactcg
     1861 tttgacctgg ctcgggctgc atccgaccag cttggccgtc ttggcaatgc tcgatccgcc
     1921 ggagcgaagc gtgatgatgc ggtcgtgcat gccggcgtca cgtttgcggc cggtgtagcg
     1981 gccggcggcc ttcgccaact ggacaccctg acgttgacgc tcgcgccgat cctcgtagtc
     2041 gtcgcgggcc atctgcaagg cgagcttcaa aagcatgtcc tggacggatt ccagaacgat
     2101 tttcgccact ccgttcgcct cggcggccag ctccgacagg tccaccacgc caggcacggc
     2161 cagcttggcc cctttggccc ggatcgacgc aaccaggcgc tcggcctcgg ccaacggcaa
     2221 gcggctgatg cggtcgatct tctccgcaac gacgacttca ccaggttgca ggtccgcgat
     2281 catgcgcagc agctcgggcc ggtcggcgcg tgcgccggac gccttctcgc ggtagatgcc
     2341 ggcgacgtag tacccggcgg cccgcgtggc cgctacaagg ctctcctggc gttcaagatt
     2401 ctgctcgtcc gtactggcgc gcaggtagat gcgggcgacc ttcaaccttc gtccctccgg
     2461 ttgttgctct cgcgtcgcca tttccacggc tcgacggcgt gcggatcgga ccagaggccg
     2521 acgcgcttgc ctcgcgcctc ctgttcgagc cgcagcattt cagggtcggc cgcgcggccg
     2581 tggaagcgat aggcccacgc catgccctgg tgaaccatcg cggcgttgac gttgcgcggc
     2641 tgcggcggcc ggctggccag ctccatgttg acccacacgg tgcccagcgt gcggccgtaa
     2701 cggtcggtgt ccttctcgtc gaccaggacg tgccggcgga acaccatgcc ggccagcgcc
     2761 tggcgcgcac gttcgccgaa ggcttgccgc ttttccggcg cgtcaatgtc caccaggcgc
     2821 acgcgcaccg gctgcttgtc taccagcacg tcgatggtgt cgccgtcgat gatgcgcacg
     2881 acctcgccgc gcagctcggc ccatgccggc gaggcaacga ccaggacggc cagcgcggca
     2941 gcggcgcgca gcatggcgta gcttcggcgc ttcatgcgtg gccccattgc tgatgatcgg
     3001 ggtacgccag gtgcagcact gcatcgaaat tggccttgca gtagccgtcc agcgccaccc
     3061 gcgagccgaa cgccggcgaa aggtactcga ccaggccggg ccggtcgcgg acctcgcgcc
     3121 ccaggacgtg gatgcgccgg ccgcgtgtgc cgtcgggtcc aggcacgaag gccagcgcct
     3181 cgatgttgaa gtcgatggat agaagttgtc ggtagtgctt ggccgccctc atcgcgtccc
     3241 ccttggtcaa attgggtata cccatttggg cctagtctag ccggcatggc gcattacagc
     3301 aatacgcaat ttaaatgcgc ctagcgcatt ttcccgacct taatgcgcct cgcgctgtag
     3361 cctcacgccc acatatgtgc taatgtggtt acgtgtattt tatggaggtt atccaatgag
     3421 ccgcctgaca atcgacatga cggaccagca gcaccagagc ctgaaagccc tggccgcctt
     3481 gcagggcaag accattaagc aatacgccct cgaacgtctg ttccccggtg acgctgatgc
     3541 cgatcaggca tggcaggaac tgaaaaccat gctggggaac cgcatcaacg atgggcttgc
     3601 cggcaaggtg tccaccaaga gcgtcggcga aattcttgat gaagaactca gcggggatcg
     3661 cgcttgacgg cctacatcct cacggctgag gccgaagccg atctacgcgg catcatccgc
     3721 tacacgcgcc gggagtgggg cgcggcgcag gtgcgccgct atatcgctaa gctggaacag
     3781 ggcatagcca ggcttgccgc cggcgaaggc ccgtttaagg acatgagcga actctttccc
     3841 gcgctgcgga tggcccgctg cgaacaccac tacgtttttt gcctgccgcg tgcgggcgaa
     3901 cccgcgttgg tcgtggcgat cctgcatgag cgcatggacc tcatgacgcg acttgccgac
     3961 aggctcaagg gctgatttca gccgctaaaa atcgcgccac tcacaacgtc ctgatggcgt
     4021 acttacccaa agaacagcta ggagaatcat ttatgctcag cacacttcca caagctcatg
     4081 caactttctt gaaccgcatc cgcgatgcgg tcgcttccga tgttcgcttc cgcgctcttc
     4141 tgatcggcgg ctcttacgtt cacggaggac tcgatgagca ctccgatttg gatttcgaca
     4201 tcgttgttga ggacaactgc tacgcagatg tcttgtctac acgcaaggat tttgccgagg
     4261 cactgcccgg cttcctcaac gcgttcaccg gcgaacatgt aggagaaccg cgccttctga
     4321 tctgcctata tggtccgcca ctgctacaca tcgggggatc agcttggcgt aatcatggtc
     4381 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     4441 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     4501 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     4561 ccaacgcgcg gggagaggcg gtttgcgtat tggaattaat tcttgaagac gaaagggcct
     4621 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agagcttatc
     4681 ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata agggacagtg
     4741 aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg ctgacgccgt
     4801 tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa
     4861 aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta tgcagcggaa
     4921 aagatccgtc gatcgaccca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4981 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5041 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5101 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5161 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5221 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5281 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     5341 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5401 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5461 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5521 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5581 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5641 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5701 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5761 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5821 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5881 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5941 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6001 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6061 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6121 gggggatcag gaccgctgcc ggagcgcaac ccactcacta cagcagagcc atgtagacaa
     6181 catcccctcc ccctttccac cgcgtcagac gcccgtagca gcccgctacg ggctttttca
     6241 tgccctgccc tagcgtccaa gcctcacggc cgcgctcggc ctctctggcg gccttctggc
     6301 gctcctgctg cggcgtccgc tcgtgggccg tggcgcgggt ccgcgcgccg gcctcgtgcg
     6361 cctggcgctc gcgggcgagg tccagggcgg ccgtcttcac gttctgcctt gcgcagatga
     6421 gatagatccg tcaattccgg cctatcgttt ccacgatcag cgatcggctc gttgccctgc
     6481 gccgctccaa agcccgcgac gcagcgccgg caggcagagc aagtagaggg cagcgcctgc
     6541 aatccatgcc cacccgttcc acgttgttat agaagccgca tagatcgccg tgaagaggag
     6601 gggtccgacg atcgaggtca ggctggtgag cgccgccagt gagccttgca gctgcccctg
     6661 acgttcctca tccacctgcc tggacaacat tgcttgcagc gccggcattc cgatgccacc
     6721 cgaagcaagc aggaccatga tcgggaacgc catccatccc cgtgtcgcga aggcaagcag
     6781 gatgtagcct gtgccgtcgg caatcattcc gagcatgagt gcccgccttt cgccgagccg
     6841 ggcggctaca gggccggtga tcattgcctg ggcgagtgaa tgcagaatgc caaatgcggc
     6901 aagcgaaatg ccgatcgtgg tcgcgtccca gtgaaagcga tcctcgccga aaatgaccca
     6961 aagcgcggcc ggcacctgtc cgacaagttg catgatgaag aagaccgcca tcagggcggc
     7021 gacgacggtc atgccccggg cccaccggaa cgaagcgagc gggttgagag cctcccggcg
     7081 taacggccgg cgttcgcctt tgtgcgactc cggcaaaagg aaacagcccg tcaggaaatt
     7141 gaggccgttc aaggctgccg cggcgaagaa cggagcgtgg ggggagaaac cgcccatcag
     7201 cccaccgagc acaggtcccg cgaccatccc gaacccgaaa caggcgctca tgaagccgaa
     7261 gtgccgcgcg cgctcatcgc catcagtgat atcggcaata taagcgccgg ctaccgcccc
     7321 agtcgccccg gtgatgccgg ccacgatccg cccgatatag agaacccaaa ggaaaggcgc
     7381 tgtcgccatg atggcgtagt cgacagtggc gccggccagc gagacgagca agattggccg
     7441 ccgcccgaaa cgatccgaca gcgcgcccag cacaggtgcg caggcaaatt gcaccaacgc
     7501 atacagcgcc agcagaatgc catagtgggc ggtgacgtcg ttcgagtgaa ccagatcgcg
     7561 caggaggccc ggcagcaccg gcataatcag gccgatgccg acagcgtcga gcgcgacagt
     7621 gctcagaatt acgatcaggg gtatgttggg tttcacgtct ggcctccgga ccagcctccg
     7681 ctggtccgat tgaacgcgcg gattctttat cactgataag ttggtggaca tattatgttt
     7741 atcagtgata aagtgtcaag catgacaaag ttgcagccga atacagtgat ccgtgccgcc
     7801 ctggacctgt tgaacgaggt cggcgtagac ggtctgacga cacgcaaact ggcggaacgg
     7861 ttgggggttc agcagccggc gctttactgg cacttcagga acaagcgggc gctgctcgac
     7921 gcactggccg aagccatgct ggcggagaat catacgcatt cggtgccgag agccgacgac
     7981 gactggcgct catttctgat cgggaatgcc cgcagcttca ggcaggcgct gctcgcctac
     8041 cgcgatggcg cgcgcatcca tgccggcacg cgaccgggcg caccgcagat ggaaacggcc
     8101 gacgcgcagc ttcgcttcct ctgcgaggcg ggtttttcgg ccggggacgc cgtcaatgcg
     8161 ctgatgacaa tcagctactt cactgttggg gccgtgcttg aggagcaggc cggcgacagc
     8221 gatgccggcg agcgcggcgg caccgttgaa caggctccgc tctcgccgct gttgcgggcc
     8281 gcgatagacg ccttcgacga agccggtccg gacgcagcgt tcgagcaggg actcgcggtg
     8341 attgtcgatg gattggcgaa aaggaggctc gttgtcagga acgttgaagg accgagaaag
     8401 ggtgacgatt gatcaggacc gctgccggag cgcaacccac tcactacagc agagccatgt
     8461 agacaacatc ccctccccct ttccaccgcg tcagacgccc gtagcagccc gctacgggct
     8521 ttttcatgcc ctgccctagc gtccaagcct cacggccgcg ctcggcctct ctggcggcct
     8581 tctggcgctc ctgctgcggc gtccgctcgt gggccgtggc gcgggtccgc gcgccggcct
     8641 cgtgcgcctg gcgctcgcgg gcgaggtcca gggcggccgt cttcacgttc tgccttgcgc
     8701 agatgagata gatcccggaa ttgacggatc tagcgtggac tcaaggctct cgcgaatggc
     8761 tcgcgttgga aactttcatt gacacttgag gggcaccgca gggaaattct cgtccttgcg
     8821 agaaccggct atgtcgtgct gcgcatcgag cctgcgccct tggcttgtct cgcccctctc
     8881 cgcgtcgcta cggggcttcc agcgcctttc cgacgctcac cgggctggtt gccctcgccg
     8941 ctgggctggc ggccgtctat ggccctgcaa acgcgccaga aacgccgtcg aagccgtgtg
     9001 cgagacaccg cggccgccgg cgttgtggat acctcgcgga aaacttggcc ctcactgaca
     9061 gatgaggggc ggacgttgac acttgagggg ccgactcacc cggcgcggcg ttgacagatg
     9121 aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa
     9181 acgcctgatt ttacgcgagt ttcccacaga tgatgtggac aagcctgggg ataagtgccc
     9241 tgcggtattg acacttgagg ggcgcgacta ctgacagatg aggggcgcga tccttgacac
     9301 ttgaggggca gagtgctgac agatgagggg cgcacctatt gacatttgag gggctgtcca
     9361 caggcagaaa atccagcatt tgcaagggtt tccgcccgtt tttcggccac cgctaacctg
     9421 tcttttaacc tgcttttaaa ccaatattta taaaccttgt ttttaaccag ggctgcgccc
     9481 tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc
     9541 gctaacgcgg gcctcccatc cccccagggg ctgcgcccct cggccgcgaa cggcctcacc
     9601 ccaaaaatgg cagcgctggc agtccttgcc attgccggga tcggggcagt aacgggatgg
     9661 gcgatcagcc cgagcgcgac gcccggaagc attgacgtgc cgcaggtgct ggcatcgaca
     9721 ttcagcgacc aggtgccggg cagtgagggc ggcggcctgg gtggcggcct gcccttcact
     9781 tcggccgtcg gggcattcac ggacttcatg gcggggccgg caatttttac cttgggcatt
     9841 cttggcatag tggtcgcggg tgccgtgctc gtgttcgggg gtgaattaat tccccggatc
     9901 gatccgtcag cttcacgctg ccgcaagcac tcagggcgca agggctgcta aaggaagcgg
     9961 aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg
    10021 gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt
    10081 acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct
    10141 ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg
    10201 ccaaggatct gatggcgcag gggatcaaga tcgacggatc gatccgggga attccggggc
    10261 aatcccgcaa ggagggtgaa tgaatcggac gtttgaccgg aaggcataca ggcaagaact
    10321 gatcgacgcg gggttttccg ccgaggatgc cgaaaccatc gcaagccgca ccgtcatgcg
    10381 tgcgccccgc gaaaccttcc agtccgtcgg ctcgatggtc cagcaagcta cggccaagat
    10441 cgagcgcgac agcgtgcaac tggctccccc tgccctgccc gcgccatcgg ccgccgtgga
    10501 gcgttcgcgt cgtctcgaac aggaggcggc aggtttggcg aagtcgatga ccatcgacac
    10561 gcgaggaact atgacgacca agaagcgaaa aaccgccggc gaggacctgg caaaacaggt
    10621 cagcgaggcc aagcaggccg cgttgctgaa acacacgaag cagcagatca aggaaatgca
    10681 gctttccttg ttcgatattg cgccgtggcc ggacacgatg cgagcgatgc caaacgacac
    10741 ggcccgctct gccctgttca ccacgcgcaa caagaaaatc ccgcgcgagg cgctgcaaaa
    10801 caaggtcatt ttccacgtca acaaggacgt gaagatcacc tacaccggcg tcgagctgcg
    10861 ggccgacgat gacgaactgg tgtggcagca ggtgttggag tacgcgaagc gcacccctat
    10921 cggcgagccg atcaccttca cgttctacga gctttgccag gacctgggct ggtcgatcaa
    10981 tggccggtat tacacgaagg ccgaggaatg cctgtcgcgc ctacaggcga cggcgatggg
    11041 cttcacgtcc gaccgcgttg ggcacctgga atcggtgtcg ctgctgcacc gcttccgcgt
    11101 cctggaccgt ggcaagaaaa cgtcccgttg ccaggtcctg atcgacgagg aaatcgtcgt
    11161 gctgtttgct ggcgaccact acacgaaatt catatgggag aagtaccgca agctgtcgcc
    11221 gacggcccga cggatgttcg actatttcag ctcgcaccgg gagccgtacc cgctcaagct
    11281 ggaaaccttc cgcctcatgt gcggatcgga ttccacccgc gtgaagaagt ggcgcgagca
    11341 ggtcggcgaa gcctgcgaag agttgcgagg cagcggcctg gtggaacacg cctgggtcaa
    11401 tgatgacctg gtgcattgca aacgctaggg ccttgtgggg tcagttccgg ctgggggttc
    11461 agcagccacc tgcaggtcat caactatcaa acgcttcggt taagcttaaa gcaactatca
    11521 aacgcttcgg ttaagcttaa agcacaccct ttctgcgtcc tcgtattgac gcgacgtaaa
    11581 atttcaacga gcacgccggg atacttacca tattctctgc taattatccc gacatcatcg
    11641 gtaacaataa atgctggata actggttgct gacgcatcca tataactcat caaccccggc
    11701 gttccatcag gtacaggttt caacgtttca ggatcaagcg ctcgcgcata tacccacggc
    11761 ggaacatgtt tacgctgcat ttcatcctca aagaaacaag tgttgagttc aacttgatta
    11821 aatatatctc ggatctgact aatatcactg agattgaaag tatcaaataa aagatgattg
    11881 aaatcatcac gtttcagaga ttctttttcg taacttttcc agccgcctcc ggttatgata
    11941 taaaggcttt tatctccaga aaatgagatt tttttatctt tcatataatg gcagagtaaa
    12001 taaataaagt atggcgaacc aataagacaa agatctttcc cttgattttt tattcgttca
    12061 agactattca atgttttaac aaaatctatt cgttcttctg ttacggtaaa tgtcgtagga
    12121 tataacaatt ccaccaaact cataacatat ttaaaccaaa tattatgagc attaaatcta
    12181 tctggtccca aattgactaa ttctatttga tgatcaaacc aactaccaac atatttcatg
    12241 ccataactca cagagcctaa gagtctctca atacttaatc tgtcacgcgc cacctgactt
    12301 tttaaaccat tcgtgccgct actggtaaac caactttcaa tctcgttttc ctgagaagtt
    12361 aataagcgag taaacttaaa aaccgatgtt gggaatacag gtatgtcatc aatttccgta
    12421 atattgtcat ctactttgtg tgcctgacag tagtgacgat attctcgaca atgtttataa
    12481 tgattacgaa atgcatcaag cacaagtttc tttctgattt tttcctgctc gtcgtaagac
    12541 cacactaatg gatcgctcga aaaaatcaaa tcatcaattt ctgagcttgc tgtaatttct
    12601 tgtttatcaa catatgaagt catacctgtt ttactcctca agataatatt agaaagtatg
    12661 gcagcactgc tgtcatactc ttttataccc ttcatctttc aagctgctgc tttgttggct
    12721 gctttcactc accccagtca catagttatc tatgctcctg gggattcgtt cacttgccgc
    12781 ctcgctgcaa ctcgaaatct attaggtata ttccatgtgg tacttcttaa tattatcatc
    12841 aacaatattg attacatttt tttggctcat caaatcattc attggttcaa aggacagcaa
    12901 tacacttttc gcaccacact tttcaattgc caacttagcc gcagttatac actccgtata
    12961 atttccgaca gcgttttctg caattatttc ttcaagttta ttttcgaaat tttcattagg
    13021 gtgcatttca agaacataat cactaataaa tgcacgcgtc tcttgtttag ctttattact
    13081 atcttcgtta tagttaacta atatcattaa ctgatggtct atctctgata ggtcaacgtc
    13141 atatttatcc gcaacggctt tatatctttc agcatattca tatctaacat cattagaatc
    13201 atcccactta aagatgagag gaataccttt tttggccgcc cactcaacaa tatgatgact
    13261 ggttgctgtt acatatttcc gaggtccgcc tggcgtataa gcatggggat ttacagatat
    13321 tttagggaag ctataaaaat cgttatctgg attacaatag cctgttgtta aagcatcgtt
    13381 aatgatttca taacactctt caaatagttg ctgttgatat tcaaccgggc gattaaaaaa
    13441 atgcatttca tctttttttt cgcaatcact aaaccctaaa ataaatctcc cttcacttaa
    13501 ctgatccaat aagcaagctt cctccgctat gcggacagga tgatgagttg taatgatgtg
    13561 atttaatgaa ccaattttaa ttttctctgt taaaccgagc agaaaaccag aaacagtcag
    13621 aggagcgccg acaacaccat tatctgaaaa atgattttca tacactaaaa tctgttcaaa
    13681 attcaactta tcaacatact ccgttatttc ctgcatgcga actatacttt gttcttgaac
    13741 agttgttgaa ttgatgaagt taaggaagaa caatccaaat ttcatttctt tctccttagc
    13801 taatataata gcgaacgttg tttttcttta agaaatggca tgacatcaga ctggaagagc
    13861 ttcatggaag caataatttc gtctactgtt ccattagctt caaatccaca acaaatattt
    13921 gatattcctg tagcatcaat gtctttttga attatgtcaa tacattcctg cggcgttccc
    13981 acgggattga tttcgtaact gtaatcaata cggcgattag tatctttatg tccttttaat
    14041 acaaagtcac gccactgccc tttattgaaa tcataacctc ttgtttggtc tgaatcatca
    14101 aaaatagtcg tagcattcac ataagaatca taccaatgcc ccagaaattt ccggcaaatc
    14161 tctttcgctt taattgagtc atgatctaca gatgttatat atgataagca atggtcgata
    14221 ttatgaatat cgtgcccata ttcttgagcc acttcattat aaagctcaag ttgtgctttc
    14281 ttttcgttag tatttataat ccaacttaat atcatcggta ggccaaattg agcagcccac
    14341 tcagtcgtcg aagctgattc agccaccaca taaaccggtg cgccacctct gctatacgcc
    14401 gcggggttta cttttacctt atggaacttg atatgttcat tatcagcttc catatatccc
    14461 tctgtcatgc cattctttat cagcccgtac cagcattccg ctaaggcgcg actgttattc
    14521 atatctgtgc cgaatacgcg aaagtccttg ttgtaaagcc ctcggcaaat accaaaccga
    14581 aatcgtcctt ttgacatttg atccaataaa ttcacatctt caagttggcg tactggatgg
    14641 gctgtgggaa gaacaatagc ggcagttcct acattcaatt ttttagtcgc gccaagtaaa
    14701 tatgcagcag cgacataagg gttaccaagc aaaccaaact ccgtgaaatg atgctccagt
    14761 aaccatacgg tatcaaaacc acactcctca gagatgcgac ctaatttaac caaacgtttc
    14821 attacctctg tttgagaaaa ttggggaggt tggtatgtaa gcaaaaagtt tccaaatttc
    14881 atagagagtc cttatattgc tatttgagtg atagaatatc tcaatagatt ttaagacaga
    14941 gaaattgctt gattttcaat ctcaattctc attcggcgtt cattgactgt cgcaatagtt
    15001 aaatgttcaa atgacggttc agtaatatca acatcaatat ccagatgatc attatccatc
    15061 gcgatagcgg ctttcgtaac cgattgataa aaattgcgca ggaccactaa attttcactc
    15121 aagtcatgcg aacttcctaa caaagaatat atcttgcatc gattactacg aatatttgat
    15181 aacaatgtga taacttcatc ttgcttgacc caattatcgt tatttgcagt aaaagcaata
    15241 aacggtatat caagatacat catgttatta attgtagaag ctaaatcttc ccaaccaaaa
    15301 tcaagacaat ctctcgcaaa gacttcagca cccaatttat ggccttcaaa atctagatta
    15361 ttcggcaatt cattaatggg tagactgaga taatcaaacc ctaaagctct ttcaagagaa
    15421 tatcttaagt taacaacacc gactgcggtg attaaaaacg aagcattgat ttcagatagg
    15481 cttgcataag ctatccgcgc agataagctt gaagccaaca taccgaagtt atttattttt
    15541 cgtgtagtta accaatcaac cactgctaac aagctctgct ttcctataga cattgtaaat
    15601 tcatcaattg tccctgaact caatccaacg tggtgaagcg aatcatagcg gatcacatga
    15661 aatccattcc gcgataaata ttccgccaga ccagcaaaat gatccatcct gcgggcaaaa
    15721 ccagacgcaa taataatggc attctttctc tttgggctgt tttcttctgg cagcgtttcc
    15781 caaacatgaa tttttttatt tccttcaaca caaataacgt ggtcgatggt tttatatttt
    15841 gattcatttt ccatactttt tacctattat gggacaaata caaggaactt atcttcttcc
    15901 aggaatcgag tctgttctat ttcaaccgca acatccttag ccgtatagtt agatggcctt
    15961 tcatgagaaa tatatgtcac taatcgttgc aacggtctca ttccgtcatg agatccacca
    16021 actcgaaata tgttattcat tcctgcttct acaatccttt ccgcaccttt taatgctaac
    16081 gcatctcgat atttaaatga tgactcccaa ggaaaaatag atatggtttg cgtcttattt
    16141 ttttgaacat aaggcaatat ttgctcaata ttatcgacgt gatgaaggta cacacatctg
    16201 ccaagtggtt gattaaattc cacacctgca tttgactcaa taatcatcca acgttgatga
    16261 atatccacct ctacttttaa tccagcaaac aagctttctt tttgaactaa agaataggcc
    16321 gccttttcat caaaatcttt tttggcattc ggtaatatat gcgcatatag attaagtttt
    16381 tctatcaacg ctaacttaaa ttcctcataa tgatttccca tgtaatatat gttttgggca
    16441 gaaaaacaag ctcgctgatc gtaaaaacaa acatcatgag ccgcacctgt cgctgcggac
    16501 gtcaaatcaa caggattatc gataatgcaa agactctttt tagaaccaaa tttaatcaca
    16561 tcagcataag atggcgcatg ctctaccgcc caattaatcg catctggccc tccccaagcg
    16621 acaataacat ccgcatgttg cataatttct tttgcgagtg atgtatcacc ttggtggggc
    16681 caatatataa cagataaaga gcgcgttatc ggatgattag ggtctacatc aataaaactt
    16741 aacgctaatg cattagcggt aaaaggatcg gttgacgatg tttttataat acactgattc
    16801 ttagttaaaa ttgcgcgtaa tatagacatg atcccagata atggaacatt acctgccaac
    16861 agatgtacag atttaccttt cggaaaagcc cgaacataac tttcatcctg aggtagccat
    16921 tcatccatga tatggcgaga accaagttca ttttctacaa catcataaag gccgccttta
    16981 gaacataaaa tcatagatat ccaattggcc tctagcttag ccatttcttc tgaatatccc
    17041 atatattttt ttaagtcacg aatgtatgtc ctgcgtcttg agtattcttc atttttccat
    17101 ctttgcccta ccgtatagag aaaattgaca atgttatgca accgtaattc gttatttcca
    17161 ttacaatcaa taatgttttt tacatgagag tcattcaata ttggcaggta aacactatta
    17221 tcaccaaaat taatggattg cactaaatca tcaccttcgg gaaagatttc aacctggccg
    17281 ttaataatga atgaaatttt tttagtcata cgtatcctcc aagcctgaat tcgtttaaac
    17341 g
//
