LOCUS       sequence-171213-        5529 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivirus vector. Expresses YFP-Ezh2 fusion proteins in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5529)
  TITLE     pTRIPZ-MSC1-Halotag-Cbx2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTRIPZ (M)-YFP-Ezh2
FEATURES             Location/Qualifiers
     source          1..5529
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        2997..4208
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4045..4064
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             4227..4934
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     misc_feature    4957..5529
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5124..5141)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5351..5370
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggcg
      721 aattcgatac gtatcgatgg cgcgcccatg ggccagactg ggaagaaatc tgagaaggga
      781 ccagtttgtt ggcggaagcg tgtaaaatca gagtacatgc gactgagaca gctcaagagg
      841 ttcagacgag ctgatgaagt aaagagtatg tttagttcca atcgtcagaa aattttggaa
      901 agaacggaaa tcttaaacca agaatggaaa cagcgaagga tacagcctgt gcacatcctg
      961 acttctgtga gctcattgcg cgggactagg gagtgttcgg tgaccagtga cttggatttt
     1021 ccaacacaag tcatcccatt aaagactctg aatgcagttg cttcagtacc cataatgtat
     1081 tcttggtctc ccctacagca gaattttatg gtggaagatg aaactgtttt acataacatt
     1141 ccttatatgg gagatgaagt tttagatcag gatggtactt tcattgaaga actaataaaa
     1201 aattatgatg ggaaagtaca cggggataga gaatgtgggt ttataaatga tgaaattttt
     1261 gtggagttgg tgaatgccct tggtcaatat aatgatgatg acgatgatga tgatggagac
     1321 gatcctgaag aaagagaaga aaagcagaaa gatctggagg atcaccgaga tgataaagaa
     1381 agccgcccac ctcggaaatt tccttctgat aaaatttttg aagccatttc ctcaatgttt
     1441 ccagataagg gcacagcaga agaactaaag gaaaaatata aagaactcac cgaacagcag
     1501 ctcccaggcg cacttcctcc tgaatgtacc cccaacatag atggaccaaa tgctaaatct
     1561 gttcagagag agcaaagctt acactccttt catacgcttt tctgtaggcg atgttttaaa
     1621 tatgactgct tcctacatcc ttttcatgca acacccaaca cttataagcg gaagaacaca
     1681 gaaacagctc tagacaacaa accttgtgga ccacagtgtt accagcattt ggagggagca
     1741 aaggagtttg ctgctgctct caccgctgag cggataaaga ccccaccaaa acgtccagga
     1801 ggccgcagaa gaggacggct tcccaataac agtagcaggc ccagcacccc caccattaat
     1861 gtgctggaat caaaggatac agacagtgat agggaagcag ggactgaaac ggggggagag
     1921 aacaatgata aagaagaaga agagaagaaa gatgaaactt cgagctcctc tgaagcaaat
     1981 tctcggtgtc aaacaccaat aaagatgaag ccaaatattg aacctcctga gaatgtggag
     2041 tggagtggtg ctgaagcctc aatgtttaga gtcctcattg gcacttacta tgacaatttc
     2101 tgtgccattg ctaggttaat tgggaccaaa acatgtagac aggtgtatga gtttagagtc
     2161 aaagaatcta gcatcatagc tccagctccc gctgaggatg tggatactcc tccaaggaaa
     2221 aagaagagga aacaccggtt gtgggctgca cactgcagaa agatacagct gaaaaaggac
     2281 ggctcctcta accatgttta caactatcaa ccctgtgatc atccacggca gccttgtgac
     2341 agttcgtgcc cttgtgtgat agcacaaaat ttttgtgaaa agttttgtca atgtagttca
     2401 gagtgtcaaa accgctttcc gggatgccgc tgcaaagcac agtgcaacac caagcagtgc
     2461 ccgtgctacc tggctgtccg agagtgtgac cctgacctct gtcttacttg tggagccgct
     2521 gaccattggg acagtaaaaa tgtgtcctgc aagaactgca gtattcagcg gggctccaaa
     2581 aagcatctat tgctggcacc atctgacgtg gcaggctggg ggatttttat caaagatcct
     2641 gtgcagaaaa atgaattcat ctcagaatac tgtggagaga ttatttctca agatgaagct
     2701 gacagaagag ggaaagtgta tgataaatac atgtgcagct ttctgttcaa cttgaacaat
     2761 gattttgtgg tggatgcaac ccgcaagggt aacaaaattc gttttgcaaa tcattcggta
     2821 aatccaaact gctatgcaaa agttatgatg gttaacggtg atcacaggat aggtattttt
     2881 gccaagagag ccatccagac tggcgaagag ctgttttttg attacagata cagccaggct
     2941 gatgccctga agtatgtcgg catcgaaaga gaaatggaaa tcccttgacg acgcgtggcc
     3001 tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc ctcacggcga gcgctgccac
     3061 gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc cggacgctca ggacagcggc
     3121 ccgctgctca taagactcgg ccttagaacc ccagtatcag cagaaggaca ttttaggacg
     3181 ggacttgggt gactctaggg cactggtttt ctttccagag agcggaacag gcgaggaaaa
     3241 gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt
     3301 atataaggac gcgccgggtg tggcacagct agttccgtcg cagccgggat ttgggtcgcg
     3361 gttcttgttt gtggatcgct gtgatcgtca cttggtgagt agcgggctgc tgggctggcc
     3421 ggggctttcg tggccgccgg gccgctcggt gggacggaag cgtgtggaga gaccgccaag
     3481 ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg ggggttgggg ggagcgcagc
     3541 aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc
     3601 gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc aaggtcttga ggccttcgct
     3661 aatgcgggaa agctcttatt cgggtgagat gggctggggc accatctggg gaccctgacg
     3721 tgaagtttgt cactgactgg agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg
     3781 gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga
     3841 cgtcacccgt tctgttggct tataatgcag ggtggggcca cctgccggta ggtgtgcggt
     3901 aggcttttct ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc ctgaatcgac
     3961 aggcgccgga cctctggtga ggggagggat aagtgaggcg tcagtttctt tggtcggttt
     4021 tatgtaccta tcttcttaag tagctgaagc tccggttttg aactatgcgc tcggggttgg
     4081 cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt gggtcaatat
     4141 gtaattttca gtgttagact agtaaattgt ccgctaaatt ctggccgttt ttggcttttt
     4201 tgttagacgc tagcggatcc gccaccatgt ctaggctgga caagagcaaa gtcataaacg
     4261 gagctctgga attactcaat ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc
     4321 aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc
     4381 tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag
     4441 gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc ataccgctgt gctctcctct
     4501 cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa
     4561 ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt
     4621 acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc
     4681 aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac
     4741 aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac
     4801 taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg accgacgccc
     4861 ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgatttt gaccttgaca
     4921 tgctccccgg gtaaggatcc gcggccgcaa attccgcccc tctccctccc ccccccctaa
     4981 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     5041 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     5101 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     5161 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     5221 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     5281 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     5341 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     5401 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     5461 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     5521 acgataata
//
