LOCUS       sequence-171217-       17119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Xylose biosensor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17119)
  AUTHORS   Pini F, East AK, Appia-Ayme C, Tomek J, Karunakaran R, 
            Mendoza-Suarez M, Edwards A, Terpolilli JJ, Roworth J, Downie JA, 
            Poole PS
  TITLE     Bacterial Biosensors for in Vivo Spatiotemporal Mapping of Root 
            Secretion.
  JOURNAL   Plant Physiol. 2017 Jul;174(3):1289-1306. doi: 10.1104/pp.16.01302. 
            Epub 2017 May 11.
  PUBMED    28495892
REFERENCE   2  (bases 1 to 17119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.16.01302"; journalName: "Plant Physiol"; date: 
            "2017-07"; volume: "174"; issue: "3"; pages: "1289-1306"
COMMENT     Sequence Label: pLMB684
FEATURES             Location/Qualifiers
     source          1..17119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(297..947)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             1053..2252
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(join(2779..3570,3571..3639))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3402..3421
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3640..3744)
                     /gene="bla"
                     /label=AmpR promoter
     oriT            3921..4029
                     /note="incP origin of transfer"
     primer_bind     4074..4092
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    4217..4238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4253..4258,4259..4276,4277..4283)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4291..4307
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4296..4318
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4315..4331
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4315..4331
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             8550..9992
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             10005..10928
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             10977..12059
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             12074..13057
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             13236..14348
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     CDS             complement(14400..15548)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      16215..16926
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tctcatctgc gcaaggcaga acgtgaagac ggccgccctg gacctcgccc gcgagcgcca
       61 ggcgcacgag gccggcgcgc ggacccgcgc cacggcccac gagcggacgc cgcagcagga
      121 gcgccagaag gccgccagag aggccgagcg cggccgtgag gcttggacgc tagggcaggg
      181 catgaaaaag cccgtagcgg gctgctacgg gcgtctgacg cggtggaaag ggggagggga
      241 tgttgtctac atggctctgc tgtagtgagt gggttgcgct ccggcagcgg tcctgatcaa
      301 tcgtcaccct ttctcggtcc ttcaacgttc ctgacaacga gcctcctttt cgccaatcca
      361 tcgacaatca ccgcgagtcc ctgctcgaac gctgcgtccg gaccggcttc gtcgaaggcg
      421 tctatcgcgg cccgcaacag cggcgagagc ggagcctgtt caacggtgcc gccgcgctcg
      481 ccggcatcgc tgtcgccggc ctgctcctca agcacggccc caacagtgaa gtagctgatt
      541 gtcatcagcg cattgacggc gtccccggcc gaaaaacccg cctcgcagag gaagcgaagc
      601 tgcgcgtcgg ccgtttccat ctgcggtgcg cccggtcgcg tgccggcatg gatgcgcgcg
      661 ccatcgcggt aggcgagcag cgcctgcctg aagctgcggg cattcccgat cagaaatgag
      721 cgccagtcgt cgtcggctct cggcaccgaa tgcgtatgat tctccgccag catggcttcg
      781 gccagtgcgt cgagcagcgc ccgcttgttc ctgaagtgcc agtaaagcgc cggctgctga
      841 acccccaacc gttccgccag tttgcgtgtc gtcagaccgt ctacgccgac ctcgttcaac
      901 aggtccaggg cggcacggat cactgtattc ggctgcaact ttgtcatgct tgacacttta
      961 tcactgataa acataatatg tccaccaact tatcagtgat aaagaatccg cgcgttcaat
     1021 cggaccagcg gaggctggtc cggaggccag acgtgaaacc caacataccc ctgatcgtaa
     1081 ttctgagcac tgtcgcgctc gacgctgtcg gcatcggcct gattatgccg gtgctgccgg
     1141 gcctcctgcg cgatctggtt cactcgaacg acgtcaccgc ccactatggc attctgctgg
     1201 cgctgtatgc gttggtgcaa tttgcctgcg cacctgtgct gggcgcgctg tcggatcgtt
     1261 tcgggcggcg gccaatcttg ctcgtctcgc tggccggcgc cactgtcgac tacgccatca
     1321 tggcgacagc gcctttcctt tgggttctct atatcgggcg gatcgtggcc ggcatcaccg
     1381 gggcgactgg ggcggtagcc ggcgcttata ttgccgatat cactgatggc gatgagcgcg
     1441 cgcggcactt cggcttcatg agcgcctgtt tcgggttcgg gatggtcgcg ggacctgtgc
     1501 tcggtgggct gatgggcggt ttctcccccc acgctccgtt cttcgccgcg gcagccttga
     1561 acggcctcaa tttcctgacg ggctgtttcc ttttgccgga gtcgcacaaa ggcgaacgcc
     1621 ggccgttacg ccgggaggct ctcaacccgc tcgcttcgtt ccggtgggcc cggggcatga
     1681 ccgtcgtcgc cgccctgatg gcggtcttct tcatcatgca acttgtcgga caggtgccgg
     1741 ccgcgctttg ggtcattttc ggcgaggatc gctttcactg ggacgcgacc acgatcggca
     1801 tttcgcttgc cgcatttggc attctgcatt cactcgccca ggcaatgatc accggccctg
     1861 tagccgcccg gctcggcgaa aggcgggcac tcatgctcgg aatgattgcc gacggcacag
     1921 gctacatcct gcttgccttc gcgacacggg gatggatggc gttcccgatc atggtcctgc
     1981 ttgcttcggg tggcatcgga atgccggcgc tgcaagcaat gttgtccagg caggtggatg
     2041 aggaacgtca ggggcagctg caaggctcac tggcggcgct caccagcctg acctcgatcg
     2101 tcggacccct cctcttcacg gcgatctatg cggcttctat aacaacgtgg aacgggtggg
     2161 catggattgc aggcgctgcc ctctacttgc tctgcctgcc ggcgctgcgt cgcgggcttt
     2221 ggagcggcgc agggcaacga gccgatcgct gatcgtggaa acgataggcc ggaattgacg
     2281 gatctatctc atctgcgcaa ggcagaacgt gaagacggcc gccctggacc tcgcccgcga
     2341 gcgccaggcg cacgaggccg gcgcgcggac ccgcgccacg gcccacgagc ggacgccgca
     2401 gcaggagcgc cagaaggccg ccagagaggc cgagcgcggc cgtgaggctt ggacgctagg
     2461 gcagggcatg aaaaagcccg tagcgggctg ctacgggcgt ctgacgcggt ggaaaggggg
     2521 aggggatgtt gtctacatgg ctctgctgta gtgagtgggt tgcgctccgg cagcggtcct
     2581 gatcccccgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2641 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     2701 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     2761 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2821 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     2881 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     2941 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3001 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3061 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3121 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     3181 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     3241 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     3301 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     3361 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
     3421 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     3481 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     3541 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     3601 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     3661 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     3721 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtcgatcga
     3781 cggatctttt ccgctgcata accctgcttc ggggtcatta tagcgatttt ttcggtatat
     3841 ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg
     3901 tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt
     3961 tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga
     4021 ggctggccga taagctctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     4081 gtatcacgag gccctttcgt cttcaagaat taattccaat acgcaaaccg cctctccccg
     4141 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     4201 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     4261 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     4321 acagctatga ccatgattac gccaagctga tcccccgatg tgtagcagtg gcggaccata
     4381 taggcagatc agaaggcgcg gttctcctac atgttcgccg gtgaacgcgt tgaggaagcc
     4441 gggcagtgcc tcggcaaaat ccttgcgtgt agacaagaca tctgcgtagc agttgtcctc
     4501 aacaacgatg tcgaaatcca aatcggagtg ctcatcgagt cctccgtgaa cgtaagagcc
     4561 gccgatcaga agagcgcgga agcgaacatc ggaagcgacc gcatcgcgga tgcggttcaa
     4621 gaaagttgca tgagcttgtg gaagtgtgct gagcataaat gattctccta gctgttcttt
     4681 gggtaagtac gccatcagga cgttgtgagt ggcgcgattt ttagcggctg aaatcagccc
     4741 ttgagcctgt cggcaagtcg cgtcatgagg tccatgcgct catgcaggat cgccacgacc
     4801 aacgcgggtt cgcccgcacg cggcaggcaa aaaacgtagt ggtgttcgca gcgggccatc
     4861 cgcagcgcgg gaaagagttc gctcatgtcc ttaaacgggc cttcgccggc ggcaagcctg
     4921 gctatgccct gttccagctt agcgatatag cggcgcacct gcgccgcgcc ccactcccgg
     4981 cgcgtgtagc ggatgatgcc gcgtagatcg gcttcggcct cagccgtgag gatgtaggcc
     5041 gtcaagcgcg atccccgctg agttcttcat caagaatttc gccgacgctc ttggtggaca
     5101 ccttgccggc aagcccatcg ttgatgcggt tccccagcat ggttttcagt tcctgccatg
     5161 cctgatcggc atcagcgtca ccggggaaca gacgttcgag ggcgtattgc ttaatggtct
     5221 tgccctgcaa ggcggccagg gctttcaggc tctggtgctg ctggtccgtc atgtcgattg
     5281 tcaggcggct cattggataa cctccataaa atacacgtaa ccacattagc acatatgtgg
     5341 gcgtgaggct acagcgcgag gcgcattaag gtcgggaaaa tgcgctaggc gcatttaaat
     5401 tgcgtattgc tgtaatgcgc catgccggct agactaggcc caaatgggta tacccaattt
     5461 gaccaagggg gacgcgatga gggcggccaa gcactaccga caacttctat ccatcgactt
     5521 caacatcgag gcgctggcct tcgtgcctgg acccgacggc acacgcggcc ggcgcatcca
     5581 cgtcctgggg cgcgaggtcc gcgaccggcc cggcctggtc gagtaccttt cgccggcgtt
     5641 cggctcgcgg gtggcgctgg acggctactg caaggccaat ttcgatgcag tgctgcacct
     5701 ggcgtacccc gatcatcagc aatggggcca cgcatgaagc gccgaagcta cgccatgctg
     5761 cgcgccgctg ccgcgctggc cgtcctggtc gttgcctcgc cggcatgggc cgagctgcgc
     5821 ggcgaggtcg tgcgcatcat cgacggcgac accatcgacg tgctggtaga caagcagccg
     5881 gtgcgcgtgc gcctggtgga cattgacgcg ccggaaaagc ggcaagcctt cggcgaacgt
     5941 gcgcgccagg cgctggccgg catggtgttc cgccggcacg tcctggtcga cgagaaggac
     6001 accgaccgtt acggccgcac gctgggcacc gtgtgggtca acatggagct ggccagccgg
     6061 ccgccgcagc cgcgcaacgt caacgccgcg atggttcacc agggcatggc gtgggcctat
     6121 cgcttccacg gccgcgcggc cgaccctgaa atgctgcggc tcgaacagga ggcgcgaggc
     6181 aagcgcgtcg gcctctggtc cgatccgcac gccgtcgagc cgtggaaatg gcgacgcgag
     6241 agcaacaacc ggagggacga aggttgaagg tcgcccgcat ctacctgcgc gccagtacgg
     6301 acgagcagaa tcttgaacgc caggagagcc ttgtagcggc cacgcgggcc gccgggtact
     6361 acgtcgccgg catctaccgc gagaaggcgt ccggcgcacg cgccgaccgg cccgagctgc
     6421 tgcgcatgat cgcggacctg caacctggtg aagtcgtcgt tgcggagaag atcgaccgca
     6481 tcagccgctt gccgttggcc gaggccgagc gcctggttgc gtcgatccgg gccaaagggg
     6541 ccaagctggc cgtgcctggc gtggtggacc tgtcggagct ggccgccgag gcgaacggag
     6601 tggcgaaaat cgttctggaa tccgtccagg acatgctttt gaagctcgcc ttgcagatgg
     6661 cccgcgacga ctacgaggat cggcgcgagc gtcaacgtca gggtgtccag ttggcgaagg
     6721 ccgccggccg ctacaccggc cgcaaacgtg acgccggcat gcacgaccgc atcatcacgc
     6781 ttcgctccgg cggatcgagc attgccaaga cggccaagct ggtcggatgc agcccgagcc
     6841 aggtcaaacg agtgtgggcg gcctggaacg cgcagcagca aaaataaagc cgggcagtgc
     6901 ccggcttttc tcaccttttc gcgtcccgca gggccgctgc gagcgcccta cctagatcct
     6961 cgctttcccc ctcggtgtag tccggccagg gcacgaaggg cgcggatgcg aacctgttga
     7021 gcaggtacgc cttcgggcag cggtagacca ccggcgagtt cgccttttca tcccaccggg
     7081 ccaggatcac gtccgcatcg cagtgcatgt ccttcacctg gtcgcggaag aagccgaagg
     7141 ccaccatgcc gctatgttcg ccgaggaacg ccagttgctt cgcgctggcg atcgcgccga
     7201 cgccgccggc caaaaccgac gccatcaccc agccgacgaa ccagaagctg gcatgcttgc
     7261 ggttgaccac cgcacgcgca gccgcgacca ggacaacggc caagctgccg accagggcca
     7321 tgacgaccgt gatccggccg ttgtggaaag cgatgggctt gccagcgtcc gcttgcacgg
     7381 cgtcgtaaat gctggacccg atgggcgcgc acatcagcac gacaggcagc agcaccagga
     7441 acatcgtccg cgtccattgc gcgagtgcct tgcggcgttc gccggcggca agcgcctcca
     7501 tcatcggcgt gaagcccaac agggccaccg cagccgccaa gccggcaacg atgccgcagg
     7561 cgattacata catacatcct ccctaatgcg ccttgcgcac ggttgtagtc agagtccgcg
     7621 gtgggggtac cctgcaaaaa ggtggaaatc tcttcctccg ggatgaagcc gcgttttgcg
     7681 agcgtcacat acagcgcgca tgcagtatgt cccttcgaaa gaacgaacct gtcgcggtcg
     7741 gggtgcttcg gttgatcggg ccaaacattc agcacacgaa aatagagggc agtcaaaatg
     7801 tcgatgaccg acatttcccc gccaatatgg ccggcgcccg cttcaaagac cgcctggaga
     7861 tcgcgcaatc ggatctcgcg agcgacccgc tcgagttctg tcgtattcat ggattcctct
     7921 catgcataaa tattcagtta tcaatatttt tgcacaagac accgattagt caagcccttt
     7981 aagtgctaca ccaacagttg caagtctaga aatcctgttg acagccggaa agcaacttgt
     8041 taatgaattt tccagcatcg gtgaatattt attcgcagaa tgaattagca gtcagcctag
     8101 gcatagggag gaacaatggt ggcgctcgat atcaatgaac acgggctttc gtccggcgcc
     8161 tggttgagca aactcaaggg agccaccggc ccgctcgtgg ggctgctcgc gctttgcgtt
     8221 ttcctgagcc tcagcaccga cacgtttctt tcggttcgga acggtctcaa catcctcgat
     8281 cagatcactg tcctcggcat catggccgtc ggaatgacct ttgtcattct gatcggcggc
     8341 atcgatcttt ccgtcggctc ggcactcgct cttgcgatga tggtcatggg ctggactgcg
     8401 aatgtcgccg gcctaccgtt gccggtcggg atcgtttttg ctctggtcgc atcgggcgtt
     8461 tcgggcctga tcgtcggact tctggtgacg cagttcaggg ttccagcgtt taggatccgt
     8521 ttaaacgaat tcaggcttgg aggatacgta tgactaaaaa aatttcattc attattaacg
     8581 gccaggttga aatctttccc gaaggtgatg atttagtgca atccattaat tttggtgata
     8641 atagtgttta cctgccaata ttgaatgact ctcatgtaaa aaacattatt gattgtaatg
     8701 gaaataacga attacggttg cataacattg tcaattttct ctatacggta gggcaaagat
     8761 ggaaaaatga agaatactca agacgcagga catacattcg tgacttaaaa aaatatatgg
     8821 gatattcaga agaaatggct aagctagagg ccaattggat atctatgatt ttatgttcta
     8881 aaggcggcct ttatgatgtt gtagaaaatg aacttggttc tcgccatatc atggatgaat
     8941 ggctacctca ggatgaaagt tatgttcggg cttttccgaa aggtaaatct gtacatctgt
     9001 tggcaggtaa tgttccatta tctgggatca tgtctatatt acgcgcaatt ttaactaaga
     9061 atcagtgtat tataaaaaca tcgtcaaccg atccttttac cgctaatgca ttagcgttaa
     9121 gttttattga tgtagaccct aatcatccga taacgcgctc tttatctgtt atatattggc
     9181 cccaccaagg tgatacatca ctcgcaaaag aaattatgca acatgcggat gttattgtcg
     9241 cttggggagg gccagatgcg attaattggg cggtagagca tgcgccatct tatgctgatg
     9301 tgattaaatt tggttctaaa aagagtcttt gcattatcga taatcctgtt gatttgacgt
     9361 ccgcagcgac aggtgcggct catgatgttt gtttttacga tcagcgagct tgtttttctg
     9421 cccaaaacat atattacatg ggaaatcatt atgaggaatt taagttagcg ttgatagaaa
     9481 aacttaatct atatgcgcat atattaccga atgccaaaaa agattttgat gaaaaggcgg
     9541 cctattcttt agttcaaaaa gaaagcttgt ttgctggatt aaaagtagag gtggatattc
     9601 atcaacgttg gatgattatt gagtcaaatg caggtgtgga atttaatcaa ccacttggca
     9661 gatgtgtgta ccttcatcac gtcgataata ttgagcaaat attgccttat gttcaaaaaa
     9721 ataagacgca aaccatatct atttttcctt gggagtcatc atttaaatat cgagatgcgt
     9781 tagcattaaa aggtgcggaa aggattgtag aagcaggaat gaataacata tttcgagttg
     9841 gtggatctca tgacggaatg agaccgttgc aacgattagt gacatatatt tctcatgaaa
     9901 ggccatctaa ctatacggct aaggatgttg cggttgaaat agaacagact cgattcctgg
     9961 aagaagataa gttccttgta tttgtcccat aataggtaaa aagtatggaa aatgaatcaa
    10021 aatataaaac catcgaccac gttatttgtg ttgaaggaaa taaaaaaatt catgtttggg
    10081 aaacgctgcc agaagaaaac agcccaaaga gaaagaatgc cattattatt gcgtctggtt
    10141 ttgcccgcag gatggatcat tttgctggtc tggcggaata tttatcgcgg aatggatttc
    10201 atgtgatccg ctatgattcg cttcaccacg ttggattgag ttcagggaca attgatgaat
    10261 ttacaatgtc tataggaaag cagagcttgt tagcagtggt tgattggtta actacacgaa
    10321 aaataaataa cttcggtatg ttggcttcaa gcttatctgc gcggatagct tatgcaagcc
    10381 tatctgaaat caatgcttcg tttttaatca ccgcagtcgg tgttgttaac ttaagatatt
    10441 ctcttgaaag agctttaggg tttgattatc tcagtctacc cattaatgaa ttgccgaata
    10501 atctagattt tgaaggccat aaattgggtg ctgaagtctt tgcgagagat tgtcttgatt
    10561 ttggttggga agatttagct tctacaatta ataacatgat gtatcttgat ataccgttta
    10621 ttgcttttac tgcaaataac gataattggg tcaagcaaga tgaagttatc acattgttat
    10681 caaatattcg tagtaatcga tgcaagatat attctttgtt aggaagttcg catgacttga
    10741 gtgaaaattt agtggtcctg cgcaattttt atcaatcggt tacgaaagcc gctatcgcga
    10801 tggataatga tcatctggat attgatgttg atattactga accgtcattt gaacatttaa
    10861 ctattgcgac agtcaatgaa cgccgaatga gaattgagat tgaaaatcaa gcaatttctc
    10921 tgtcttaaaa tctattgaga tattctatca ctcaaatagc aatataagga ctctctatga
    10981 aatttggaaa ctttttgctt acataccaac ctccccaatt ttctcaaaca gaggtaatga
    11041 aacgtttggt taaattaggt cgcatctctg aggagtgtgg ttttgatacc gtatggttac
    11101 tggagcatca tttcacggag tttggtttgc ttggtaaccc ttatgtcgct gctgcatatt
    11161 tacttggcgc gactaaaaaa ttgaatgtag gaactgccgc tattgttctt cccacagccc
    11221 atccagtacg ccaacttgaa gatgtgaatt tattggatca aatgtcaaaa ggacgatttc
    11281 ggtttggtat ttgccgaggg ctttacaaca aggactttcg cgtattcggc acagatatga
    11341 ataacagtcg cgccttagcg gaatgctggt acgggctgat aaagaatggc atgacagagg
    11401 gatatatgga agctgataat gaacatatca agttccataa ggtaaaagta aaccccgcgg
    11461 cgtatagcag aggtggcgca ccggtttatg tggtggctga atcagcttcg acgactgagt
    11521 gggctgctca atttggccta ccgatgatat taagttggat tataaatact aacgaaaaga
    11581 aagcacaact tgagctttat aatgaagtgg ctcaagaata tgggcacgat attcataata
    11641 tcgaccattg cttatcatat ataacatctg tagatcatga ctcaattaaa gcgaaagaga
    11701 tttgccggaa atttctgggg cattggtatg attcttatgt gaatgctacg actatttttg
    11761 atgattcaga ccaaacaaga ggttatgatt tcaataaagg gcagtggcgt gactttgtat
    11821 taaaaggaca taaagatact aatcgccgta ttgattacag ttacgaaatc aatcccgtgg
    11881 gaacgccgca ggaatgtatt gacataattc aaaaagacat tgatgctaca ggaatatcaa
    11941 atatttgttg tggatttgaa gctaatggaa cagtagacga aattattgct tccatgaagc
    12001 tcttccagtc tgatgtcatg ccatttctta aagaaaaaca acgttcgcta ttatattagc
    12061 taaggagaaa gaaatgaaat ttggattgtt cttccttaac ttcatcaatt caacaactgt
    12121 tcaagaacaa agtatagttc gcatgcagga aataacggag tatgttgata agttgaattt
    12181 tgaacagatt ttagtgtatg aaaatcattt ttcagataat ggtgttgtcg gcgctcctct
    12241 gactgtttct ggttttctgc tcggtttaac agagaaaatt aaaattggtt cattaaatca
    12301 catcattaca actcatcatc ctgtccgcat agcggaggaa gcttgcttat tggatcagtt
    12361 aagtgaaggg agatttattt tagggtttag tgattgcgaa aaaaaagatg aaatgcattt
    12421 ttttaatcgc ccggttgaat atcaacagca actatttgaa gagtgttatg aaatcattaa
    12481 cgatgcttta acaacaggct attgtaatcc agataacgat ttttatagct tccctaaaat
    12541 atctgtaaat ccccatgctt atacgccagg cggacctcgg aaatatgtaa cagcaaccag
    12601 tcatcatatt gttgagtggg cggccaaaaa aggtattcct ctcatcttta agtgggatga
    12661 ttctaatgat gttagatatg aatatgctga aagatataaa gccgttgcgg ataaatatga
    12721 cgttgaccta tcagagatag accatcagtt aatgatatta gttaactata acgaagatag
    12781 taataaagct aaacaagaga cgcgtgcatt tattagtgat tatgttcttg aaatgcaccc
    12841 taatgaaaat ttcgaaaata aacttgaaga aataattgca gaaaacgctg tcggaaatta
    12901 tacggagtgt ataactgcgg ctaagttggc aattgaaaag tgtggtgcga aaagtgtatt
    12961 gctgtccttt gaaccaatga atgatttgat gagccaaaaa aatgtaatca atattgttga
    13021 tgataatatt aagaagtacc acatggaata tacctaatag atttcgagtt gcagcgaggc
    13081 ggcaagtgaa cgaatcccca ggagcataga taactatgtg actggggtga gtgaaagcag
    13141 ccaacaaagc agcagcttga aagatgaagg gtataaaaga gtatgacagc agtgctgcca
    13201 tactttctaa tattatcttg aggagtaaaa caggtatgac ttcatatgtt gataaacaag
    13261 aaattacagc aagctcagaa attgatgatt tgattttttc gagcgatcca ttagtgtggt
    13321 cttacgacga gcaggaaaaa atcagaaaga aacttgtgct tgatgcattt cgtaatcatt
    13381 ataaacattg tcgagaatat cgtcactact gtcaggcaca caaagtagat gacaatatta
    13441 cggaaattga tgacatacct gtattcccaa catcggtttt taagtttact cgcttattaa
    13501 cttctcagga aaacgagatt gaaagttggt ttaccagtag cggcacgaat ggtttaaaaa
    13561 gtcaggtggc gcgtgacaga ttaagtattg agagactctt aggctctgtg agttatggca
    13621 tgaaatatgt tggtagttgg tttgatcatc aaatagaatt agtcaatttg ggaccagata
    13681 gatttaatgc tcataatatt tggtttaaat atgttatgag tttggtggaa ttgttatatc
    13741 ctacgacatt taccgtaaca gaagaacgaa tagattttgt taaaacattg aatagtcttg
    13801 aacgaataaa aaatcaaggg aaagatcttt gtcttattgg ttcgccatac tttatttatt
    13861 tactctgcca ttatatgaaa gataaaaaaa tctcattttc tggagataaa agcctttata
    13921 tcataaccgg aggcggctgg aaaagttacg aaaaagaatc tctgaaacgt gatgatttca
    13981 atcatctttt atttgatact ttcaatctca gtgatattag tcagatccga gatatattta
    14041 atcaagttga actcaacact tgtttctttg aggatgaaat gcagcgtaaa catgttccgc
    14101 cgtgggtata tgcgcgagcg cttgatcctg aaacgttgaa acctgtacct gatggaacgc
    14161 cggggttgat gagttatatg gatgcgtcag caaccagtta tccagcattt attgttaccg
    14221 atgatgtcgg gataattagc agagaatatg gtaagtatcc cggcgtgctc gttgaaattt
    14281 tacgtcgcgt caatacgagg acgcagaaag ggtgtgcttt aagcttaacc gaagcgtttg
    14341 atagttgatg acctgcaggt ggctgctgaa cccccagccg gaactgaccc cacaaggccc
    14401 tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag gccgctgcct
    14461 cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc
    14521 gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg gtgcgagctg
    14581 aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc ccatatgaat
    14641 ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag gacctggcaa
    14701 cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga caccgattcc
    14761 aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat
    14821 tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg gcaaagctcg
    14881 tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc caacacctgc
    14941 tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg
    15001 tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg
    15061 cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc
    15121 cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt cgtgtgtttc
    15181 agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc ggcggttttt
    15241 cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc
    15301 gcctcctgtt cgagacgacg cgaacgctcc acggcggccg atggcgcggg cagggcaggg
    15361 ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctggac catcgagccg
    15421 acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca
    15481 tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc
    15541 cgattcattc accctccttg cgggattgcc ccggaattcc ccggatcgat ccgtcgatct
    15601 tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg
    15661 cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa
    15721 aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc
    15781 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg
    15841 tttctgcgga ctggctttct acgtgttccg cttcctttag cagcccttgc gccctgagtg
    15901 cttgcggcag cgtgaagctg acggatcgat ccggggaatt aattcacccc cgaacacgag
    15961 cacggcaccc gcgaccacta tgccaagaat gcccaaggta aaaattgccg gccccgccat
    16021 gaagtccgtg aatgccccga cggccgaagt gaagggcagg ccgccaccca ggccgccgcc
    16081 ctcactgccc ggcacctggt cgctgaatgt cgatgccagc acctgcggca cgtcaatgct
    16141 tccgggcgtc gcgctcgggc tgatcgccca tcccgttact gccccgatcc cggcaatggc
    16201 aaggactgcc agcgctgcca tttttggggt gaggccgttc gcggccgagg ggcgcagccc
    16261 ctggggggat gggaggcccg cgttagcggg ccgggagggt tcgagaaggg ggggcacccc
    16321 ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca aggtttataa
    16381 atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac gggcggaaac
    16441 ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc
    16501 ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag
    16561 tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc cacatcatct
    16621 gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc cagctccacg
    16681 tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc
    16741 ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc gcgaggtatc
    16801 cacaacgccg gcggccgcgg tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg
    16861 cagggccata gacggccgcc agcccagcgg cgagggcaac cagcccggtg agcgtcggaa
    16921 aggcgctgga agccccgtag cgacgcggag aggggcgaga caagccaagg gcgcaggctc
    16981 gatgcgcagc acgacatagc cggttctcgc aaggacgaga atttccctgc ggtgcccctc
    17041 aagtgtcaat gaaagtttcc aacgcgagcc attcgcgaga gccttgagtc cacgctagat
    17101 ccgtcaattc cgggatcta
//
