LOCUS       sequence-171218-        9493 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivirus vector. Expresses YFP-Eed fusion proteins in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9493)
  TITLE     pTRIPZ-MSC1-Halotag-Cbx2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTRIPZ (M)-YFP-Eed
FEATURES             Location/Qualifiers
     source          1..9493
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      152..740
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     641..660
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     894..911
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1028..1049
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1064..1069,1070..1087,1088..1094)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1102..1118
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1107..1129
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1126..1142
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1126..1142
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1191..1312
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1202..1221)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1256..1275
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(2520..2849)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2534..2669
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2588..2607)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2834..2854
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2863..3291)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2973..2994)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3185..3204
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3337..3561
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3550..3567
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             3668..3901
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4108..4696
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4214..4225
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4623..4643
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4712..5314)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4795..4815)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5317..5889
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5476..5495)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     5705..5722
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(5912..6619)
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     promoter        complement(6638..7849)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(6782..6801)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             complement(9094..9345)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(9136..9157)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
       61 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
      121 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
      181 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
      241 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
      301 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
      361 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
      421 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
      481 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
      541 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      601 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      661 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      721 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
      781 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      841 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
      901 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
      961 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     1021 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     1081 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     1141 accatgatta cgccaagctc tagctagagg tcgacggtat acagacatga taagatacat
     1201 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     1261 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttggggtggg
     1321 cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa
     1381 cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtagcacgt
     1441 gctattcctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt
     1501 ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag
     1561 tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga
     1621 aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc
     1681 ggagccgcgg cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct
     1741 ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc
     1801 cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat
     1861 tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa
     1921 gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt
     1981 gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt
     2041 gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag
     2101 cgatcgcatc catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca
     2161 ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga
     2221 attaattccc caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga
     2281 taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca
     2341 cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg
     2401 tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca
     2461 ggctttttca tcacgtgctg atcagatccg aaaatggata tacaagctcc cgggagcttt
     2521 ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggca
     2581 gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg
     2641 gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct
     2701 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
     2761 cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct
     2821 ggggactttc cacaccctaa ctgacacaca ttccacagaa ttaattcgcg ttaaattttt
     2881 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     2941 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     3001 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     3061 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     3121 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     3181 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     3241 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tggggatacc
     3301 ccctagagcc ccagctggtt ctttccgcct cagaagccat agagcccacc gcatccccag
     3361 catgcctgct attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca
     3421 gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga
     3481 cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg
     3541 gctggcaact agaaggcaca gtcgaggctg atcagcgggt ttaaacaatg tcaaggcctc
     3601 tcactctctg atattcattt ctttgcaagt tataaatact gaataataag atgacatgaa
     3661 ctactactgc tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca
     3721 gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc
     3781 ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag
     3841 agagacccag tacaagcaaa aagcagatct tgtcttcgtt gggagtgaat tagcccttcc
     3901 agtcccctct tttcttttaa aaagtggcta agatctacag ctgccttgta agtcattggt
     3961 cttaaaggta cctgaggtgt gactggaaaa cccacctcct cctcctcttg tgcttctagc
     4021 caggcacaat cagcattggt agctgctgta ttgctacttg tgattgctcc atgtttttct
     4081 aggtctcgac tgcagaatta attccaggcg gggaggcggc ccaaagggag atccgactcg
     4141 tctgagggcg aaggcgaaga cgcggaagag gccgcagagc cggcagcagg ccgcgggaag
     4201 gaaggtccgc tggattgagg gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc
     4261 aggtggcaac acaggcgagc agccatggaa aggacgtcag cttccccgac aacaccacgg
     4321 aattgtcagt gcccaacagc cgagcccctg tccagcagcg ggcaaggcag gcggcgatga
     4381 gttccgccgt ggcaataggg agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg
     4441 tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc acgccacgtt
     4501 gcctgacaac gggccacaac tcctcataaa gagacagcaa ccaggattta tacaaggagg
     4561 agaaaatgaa agccatacgg gaagcaatag catgatacaa aggcattaaa gcagcgtatc
     4621 cacatagcgt aaaaggagca acatagttaa gaataccagt caatctttca caaattttgt
     4681 aatccagagg ttgattgttc cagacgcgaa ctcaggcacc gggcttgcgg gtcatgcacc
     4741 aggtgcgcgg tccttcgggc acctcgacgt cggcggtgac ggtgaagccg agccgctcgt
     4801 agaaggggag gttgcggggc gcggaggtct ccaggaaggc gggcacccca gcgcgctcgg
     4861 ccgcctccac tccggggagc acgacggcgc tgcccagacc cttgccctgg tggtcgggcg
     4921 agacgccgac ggtggccagg aaccacgcgg gctccttggg ccggtgcggc gccaggaggc
     4981 cttccatctg ttgctgcgcg gccagccggg aaccgctcaa ctcggccatg cgcgagccga
     5041 tctcggcgaa caccgccccc gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg
     5101 cgccgtcgtc cgcgacccac accttgccga tgtcgagccc gacgcgcgtg aggaagagtt
     5161 cttgcagctc ggtgacccgc tcgatgtggc ggtccgggtc gacggtgtgg cgcgtggcgg
     5221 ggtagtcggc gaacgcggcg gcgagggtgc gtacggcccg ggggacgtcg tcgcgggtgg
     5281 cgaggcgcac cgtgggcttg tactcggtgg ccatggtatt atcgtgtttt tcaaaggaaa
     5341 accacgtccc cgtggttcgg ggggcctaga cgttttttta acctcgacta aacacatgta
     5401 aagcatgtgc accgaggccc cagatcagat cccatacaat ggggtacctt ctgggcatcc
     5461 ttcagcccct tgttgaatac gcttgaggag agccatttga ctctttccac aactatccaa
     5521 ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg tatcttatac acgtggcttt
     5581 tggccgcaga ggcacctgtc gccaggtggg gggttccgct gcctgcaaag ggtcgctaca
     5641 gacgttgttt gtcttcaaga agcttccaga ggaactgctt ccttcacgac attcaacaga
     5701 ccttgcattc ctttggcgag aggggaaaga cccctaggaa tgctcgtcaa gaagacaggg
     5761 ccaggtttcc gggccctcac attgccaaaa gacggcaata tggtggaaaa taacatatag
     5821 acaaacgcac accggcctta ttccaagcgg cttcggccag taacgttagg ggggggggag
     5881 ggagaggggc ggaatttgcg gccgcggatc cttacccggg gagcatgtca aggtcaaaat
     5941 cgtcaagggc atcggctggg agcatgtcta agtcaaaatc gtcaagggcg tcggtcggcc
     6001 cgccgctttc gcactttagc tgtttctcca ggccacatat gattagttcc aggccgaaaa
     6061 ggaaggcagg ttcggctccc tgccggtcga acagctcaat tgcttgtctc agaagtgggg
     6121 gcatagaatc ggtggtaggt gtctctcttt cctcttttgc tacttgatgc tcctgttcct
     6181 ccaatacgca gcccagtgta aagtggccca cggcggacag agcgtacagt gcgttctcca
     6241 gggagaagcc ttgctgacac aggaacgcga gctgattttc cagggtttcg tactgtttct
     6301 ctgttgggcg ggtgccgaga tgcactttag ccccgtcgcg atgtgagagg agagcacagc
     6361 ggtatgactt ggcgttgttc cgcagaaagt cttgccatga ctcgccttcc agggggcaga
     6421 agtgggtatg atgcctgtcc agcatctcga ttggcagggc atcgagcagg gcccgcttgt
     6481 tcttcacgtg ccagtacagg gtaggctgct caactcccag cttttgagcg agtttccttg
     6541 tcgtcaggcc ttcgataccg acaccattga gtaattccag agctccgttt atgactttgc
     6601 tcttgtccag cctagacatg gtggcggatc cgctagcgtc taacaaaaaa gccaaaaacg
     6661 gccagaattt agcggacaat ttactagtct aacactgaaa attacatatt gacccaaatg
     6721 attacatttc aaaaggtgcc taaaaaactt cacaaaacac actcgccaac cccgagcgca
     6781 tagttcaaaa ccggagcttc agctacttaa gaagataggt acataaaacc gaccaaagaa
     6841 actgacgcct cacttatccc tcccctcacc agaggtccgg cgcctgtcga ttcaggagag
     6901 cctaccctag gcccgaaccc tgcgtcctgc gacggagaaa agcctaccgc acacctaccg
     6961 gcaggtggcc ccaccctgca ttataagcca acagaacggg tgacgtcacg acacgacgag
     7021 ggcgcgcgct cccaaaggta cgggtgcact gcccaacggc accgccataa ctgccgcccc
     7081 cgcaacagac gacaaaccga gttctccagt cagtgacaaa cttcacgtca gggtccccag
     7141 atggtgcccc agcccatctc acccgaataa gagctttccc gcattagcga aggcctcaag
     7201 accttgggtt cttgccgccc accatgcccc ccaccttgtt tcaacgacct cacagcccgc
     7261 ctcacaagcg tcttccattc aagactcggg aacagccgcc attttgctgc gctcccccca
     7321 acccccagtt cagggcaacc ttgctcgcgg acccagacta cagcccttgg cggtctctcc
     7381 acacgcttcc gtcccaccga gcggcccggc ggccacgaaa gccccggcca gcccagcagc
     7441 ccgctactca ccaagtgacg atcacagcga tccacaaaca agaaccgcga cccaaatccc
     7501 ggctgcgacg gaactagctg tgccacaccc ggcgcgtcct tatataatca tcggcgttca
     7561 ccgccccacg gagatccctc cgcagaatcg ccgagaaggg actacttttc ctcgcctgtt
     7621 ccgctctctg gaaagaaaac cagtgcccta gagtcaccca agtcccgtcc taaaatgtcc
     7681 ttctgctgat actggggttc taaggccgag tcttatgagc agcgggccgc tgtcctgagc
     7741 gtccgggcgg aaggatcagg acgctcgctg cgcccttcgt ctgacgtggc agcgctcgcc
     7801 gtgaggaggg gggcgcccgc gggaggcgcc aaaacccggc gcggaggcca cgcgtcgtta
     7861 tcgaagtcga tcccatcgcc aaatgctggc atcatcgcag acagctatga ggatgctgct
     7921 atccctactg aaactggttt gtcgaatagc cgcgccacat ttatgatggg tcagtgttgt
     7981 gcatttggct ttatgaggat cttctacttc taaatcccaa acatacagtt tgccaacctg
     8041 attgcccaat gcaagcatct tttgccagaa atccatagaa aacctcatgt accaaatgtc
     8101 acactggctg taatcaaatc gcccaagaat agtcacatta gactcactag gtttaatttt
     8161 atctatatca tcctccattt tgccaggttt ccagcataca atggcatttt cacaagactt
     8221 ggaaagtatc aaatcgccta accatcgcac acaatcaaca taattcctat gtatgtctct
     8281 ggtagaaaag tcaggaaagt ggattttctg ggaaataaat ggcctgttag ttttgtttgg
     8341 gttataatca taagactcct taattgcatt catcatcctc tttgagttga ttctccacag
     8401 tttaagagag tgatccatac cacaggacat tattttttca cccaaaagat cataatcagc
     8461 actcagaact tcatctctgt gcccttccac acctccgaat attgccacaa gagtgtctgt
     8521 ttggatattc cataaccgta aagcatgatc tttacttact gacaggagaa ggtttgggtc
     8581 tcgtgggtgg aatttcagct cattgatagc atttccatgg ccaacatagt gctttataca
     8641 ctgcattgtt ataggattaa ttattcttat aatgcctcta gatccagcaa ctgctaatag
     8701 agggtggctg gtgttgctat cataggtcca tgcacaagtg taaaagtttt catctgcatc
     8761 agcatctaca taggactgca ataaccgtat ctccccctgt gaatggcatt cgtataaggt
     8821 tactctgttg cttcccacag ttgcaaacac cagagggtct ccttctttac tgtgccagtt
     8881 aaactgaact ccaaacaatg gctggttatg atcttccttg aggctgttca cacatttgaa
     8941 agaatatttg catttctttg acttccattt tccctttccc cagcttttcc ttcctggtgc
     9001 atttggcgta tttgtgggcg tgtccgggcg ttctgtgttt gtgccactct caatactgac
     9061 agcatcgtcc tcgagcatag caagtgaatt cgccttgtac agctcgtcca tgccgagagt
     9121 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
     9181 tttgctcagg gcggactggt agctcaggta gtggttgtcg ggcagcagca cggggccgtc
     9241 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt
     9301 gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac
     9361 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
     9421 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc
     9481 gcgggtcttg tag
//
