LOCUS       sequence-171221-        5306 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivirus vector. Expresses YFP-Eed fusion proteins in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5306)
  TITLE     pTRIPZ-MSC1-Halotag-Cbx2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTRIPZ (M)-YFP-Eed
FEATURES             Location/Qualifiers
     source          1..5306
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     139..158
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     398..419
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      461..470
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(489..513)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(510..548)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    560..578
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    596..614
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    631..649
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    667..685
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    703..721
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    738..756
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        792..839
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        831..849
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      853..862
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             859..1233
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    1298..1415
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1713..1757)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1942..2175
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2672..2797
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2844..3477
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    3645..3779
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3669..3688)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3723..3742
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        3827..3931
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3932..4000,4001..4792)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4150..4169)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg
       61 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
      121 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
      181 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
      241 tagcgggcga agcactgcag gccgtagccg aaggtggtca cgagggtggg ccagggcacg
      301 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
      361 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
      421 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtggc cctcgagcgg
      481 accggtgcga tctgacggtt cactaaacga gctctgctta tataggcctc ccaccgtaca
      541 cgcctacctc gacatacgtt ctctatcact gatagggagt aaactcgaca tacgttctct
      601 atcactgata gggataaact cgacatacgt tctctatcac tgatagggag taaactcgac
      661 atacgttctc tatcactgat agggagtaaa ctcgacatac gttctctatc actgataggg
      721 agtaaactcg acatcgttct ctatcactga tagggagtaa actcgtctag aacctcggac
      781 ctgcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgact
      841 cactatagga gggccaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
      901 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
      961 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     1021 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     1081 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     1141 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     1201 tgcgtgcact tcgtggccga ggagcaggac tgattccgga ctagagcggg cccggtacta
     1261 accaaactgg atctctgctg tccctgtaat aaacccgaaa attttgaatt tttgtaattt
     1321 gtttttgtaa ttctttagtt tgtatgtctg ttgctattat gtctactatt ctttcccctg
     1381 cactgtaccc cccaatcccc ccttttcttt taaaattgtg gatgaatact gccatttgtc
     1441 tgcagaattg gcgcacgcag tgccgatccg ttcactaatc gaatggatct gtctctgtct
     1501 ctctctccac cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg ggttgggagg
     1561 tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat agaaagtaca
     1621 gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa ttttatatac
     1681 cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc taattccaat
     1741 aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga gtgtattaag
     1801 cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt
     1861 tccagagatt tattactcca actagcattc caaggcacag cagtggtgca aatgagtttt
     1921 ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc cacagccagg
     1981 attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg ctgttgcgcc
     2041 tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa ttgtctggcc
     2101 tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc caagaaccca
     2161 aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct tctctttgcc
     2221 ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata taattcactt
     2281 ctccaattgt ccctcatatc tcctcctcca ggtctgaaga tcagcggccg gccgcttgct
     2341 gtgcggtggt cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt
     2401 cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc
     2461 taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc
     2521 cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc
     2581 atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc
     2641 atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct
     2701 agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg
     2761 tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt
     2821 tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag
     2881 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
     2941 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
     3001 gatctggtct aaccagagag acccagtaca ggcaaaaagc agctgcttat atgcaggatc
     3061 tgagggctcg ccactcccca gtcccgccca ggccacgcct ccctggaaag tccccagcgg
     3121 aaagtccctt gtagcaagct cgatatcagc agttcttgaa gtactccgga tgcagctctc
     3181 gggccacgtg atgaaatgct aggcggctgt caaacctcca ctctaacact tctctctccg
     3241 ggtcatccat cccatgcagg ctcacagggt gtaacaagct ggtgttctct cctttattgg
     3301 cctcttctac cttatctggc tcaactggta ctagcttgta gcaccatcca aaggtcagtg
     3361 gatatctgac ccctggccct ggtgtgtagt tctgctaatc agggaagtag ccttgtgtgt
     3421 ggtagatcca cagatcaagg atatcttgtc ttctttggga gtgaattagc ccttccaact
     3481 actaagtttg tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag
     3541 gaatcacagg tttagagtaa atgaaaccac aggtaattgg cagtggtaat agggtatggg
     3601 gtgggaagtt tgggatgatt ttggttagct tgagttatcc agttgatcca gacatgataa
     3661 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     3721 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagttg
     3781 atctcccgat ccgtcgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3841 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3901 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     3961 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4021 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4081 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4141 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4201 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4261 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4321 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4381 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4441 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     4501 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4561 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4621 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4681 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4741 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     4801 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4861 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4921 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4981 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5041 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5101 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5161 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5221 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5281 aacggggggt tcgtgcacac agccca
//
